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Citations for Thermo Fisher :
501 - 550 of 6145 citations for GDF 11 BMP 11 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Ly6A/E(Sca-1)-PE (Clone D7, cat. # 12-5981-82), CD16/CD32-FITC (clone 93, cat. # 11-0161-82) were purchases from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The concentration of the Cas7-11–Csx29–crRNA complex was measured using Pierce 660-nm Protein Assay Reagent (Thermo Fisher Scientific). Csx30 was expressed in E ...
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bioRxiv - Molecular Biology 2022Quote: ... using sterile surgical blade (Size: 11) by immobilizing the insect on ice and collected antennae into 1.5mL Eppendorf tube containing RNAlater® solution (Thermofisher, USA). Fifty pairs of weevil antennae were used per replicate ...
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bioRxiv - Neuroscience 2022Quote: ... Peptides from each individual case and the GIS pooled standard or bridging sample (at least one per batch) were labeled using the TMT 11-plex kit (ThermoFisher 90406). Labeling was performed as described (18 ...
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bioRxiv - Genomics 2022Quote: ... DNA quantity was checked by fluorometry on the DS-11 FX instrument (DeNovix) with the Qubit dsDNA HS Assay Kit (Thermo Fisher) and sizes were examined on FEMTO Pulse (Agilent Technologies ...
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bioRxiv - Immunology 2022Quote: ... RNA was eluted from the magnetic beads with 11 μL of a solution containing 14.5 ng/μL of random primers (Invitrogen, Cat # 48190011), 0.5% of Igepal Ca-630 (type NP-40 ...
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bioRxiv - Microbiology 2022Quote: ... 1mM DTT, 300 mM KCl, 20µM biotin-11-ATP, -UTP, -GTP, -CTP, 0.4 U/ml RNase inhibitor [RNaseOUT ThermoFisher] and 1% Sarkosyl) was added to thawed nuclei ...
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bioRxiv - Molecular Biology 2022Quote: ... non-coated 35 mm dish and single fibers were gently released with serial trituration into 2 mL of DMEM (catalog number 11-965-092, Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... The 14.4.4 mAb and the I-Ak-reactive 11-5.2 mAb were randomly labeled via available lysine residues with Alexa Fluor 647 carboxylic acid succinimidyl ester (Thermo Fisher Scientific) according to the manufacturer’s instructions and purified via gel filtration (Superdex-200 10/300 GL ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides from each individual case and the GIS pooled standard or bridging sample were labeled using the TMT 11-plex kit (ThermoFisher 90406). For each batch ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with either scrambled (non-targeting #1) siRNA or Septin9 siRNA (J-048947-11-0050 ONTARGETplus) with Lipofectamine-iMAX (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested for up to 11 days and the cell number recorded using a Countess IIR automated cell counter (Life Technologies).
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bioRxiv - Immunology 2023Quote: SVG-A and 293T cells were infected with JCPyV M1-SVEΔ for 9-11 days and stained first with the LIVE/DEAD® Fixable Aqua Dead Cell Stain Kit (Invitrogen), then cells were split and stained in parallel with one of the following antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... we washed and rested islets overnight in islet medium (RPMI 1640 with 11 mM glucose, 10% FBS, and 1% Penicillin-Streptomycin (Gibco, #15140122)) ...
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bioRxiv - Microbiology 2023Quote: ... For FcγRII we stained with anti-CD32-FITC (Cat# 60012.FI, StemCell) and corresponding mouse IgG2b-FITC isotype control (Cat# 11-4732-81, ThermoFisher Scientific). For FcαRI we stained with anti-CD89/-PE (cat# 555686 ...
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bioRxiv - Molecular Biology 2023Quote: Differentiated EB cells were desegregated and stained with the anti-mouse CD71-APC antibody (eBioscience, 11-0711-80) (1:8000 in staining buffer) and Hoechst (Invitrogen, 33258) (1:10000 in staining buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA quantity was tested with fluorometry on a DS-11 FX instrument (DeNovix) with the Qubit dsDNA HS Assay Kit (Thermo Fisher). Sizes were analyzed on a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µL was loaded onto an IonPac AS-11 HC strong anion-exchange analytical column (2 mm × 250 mm, 4 µm particle diameter, Thermo Scientific) with AG-11 HC guard column (2 mm × 50 mm ...
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bioRxiv - Microbiology 2023Quote: ... DNA concentration of eluates was assessed using a DS-11 FX+ Spectrophotometer (Denovix) using a Qubit™ dsDNA High Sensitivity Assay Kit (Invitrogen). 200 ng of each individually extracted sample was used to pool by cage (4 samples sent for sequencing).
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bioRxiv - Systems Biology 2023Quote: We conducted mass spectrometry analysis of 120 CSF samples using 13 batches of 11-plex tandem mass tag (TMT, Thermo Scientific) experiments ...
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bioRxiv - Immunology 2024Quote: ... CD3 FITC (clone OKT3, catalog #: 11-0037-42) and CD66b PE-Cy7 (clone G10F5, catalog #: 25-0666-42) from Thermo Scientific; CD56 FITC (clone MEM-188 ...
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bioRxiv - Genomics 2024Quote: ... DNA quantity was checked with fluorometry on the DS-11 FX instrument (DeNovix) with the Qubit dsDNA HS Assay Kit (ThermoFisher Scientific) and sizes were examined on a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were collected by centrifugation and labelled using tandem mass tag (TMT) 11-plex isobaric mass tagging reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and reverse transfected with 1 pmol/well of a combination of 3 non-overlapping 11-mer siRNAs (Ambion Silencer® Select, Thermofisher) using Lipofectamine RNAiMax transfection reagent (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: Human embryonic kidney cells 293 (HEK293) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS ...
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An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: HMC3 human microglia or HEK293 cells were transfected with Lipofectamine 3000 with Plus reagent (Invitrogen L3000001) per manufacturer instructions with 0.8 μL of Lipofectamine 3000 ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmids were transfected into HEK293 human embryonic kidney cells using Lipofectamine2000 (Invitrogen, Carlsbad, CA, USA). The recombinant proteins were purified with anti-FLAG agarose affinity gel (A2220 ...
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bioRxiv - Biochemistry 2021Quote: Human HEK293S GnTI- cells were cultured as suspension at 37°C in FreeStyle293 expression medium (Invitrogen) supplemented with 2% (vol/vol ...
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bioRxiv - Neuroscience 2022Quote: Human embryonic kidney 293 cells (HEK293, ECACC) were cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher) with 10% fetal bovine serum (Hyclone) ...
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bioRxiv - Cell Biology 2022Quote: Human embryonic kidney (HEK293) cells from ATCC were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney cells (HEK293) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco #12430-054) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: Human embryonic kidney (HEK293, HEK293A) cell lines were grown in Dulbeco’s Modified Eagle medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... Rabbit anti-Myelin Basic Protein (BMP) (ThermoFisher; 1:300), Mouse anti-NeuN (Abcam ...
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bioRxiv - Cell Biology 2020Quote: Cells were treated with the siRNA pool targeting FCHSD2 (#J-021240-10, J-021240-11, J-021240-12, Dharmacon) or Rab7 (LU-010388-00, Dharmacon) using RNAiMAX (Thermo Fisher Scientific) to silence the endogenous protein ...
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bioRxiv - Genetics 2021Quote: ... Differentiation efficiency was quantified using flow cytometry as described previously 59 using mouse monoclonal antibody against cardiac troponin-T (clone 13-11, Thermo Fisher Scientific). All iPS-CM cultures used in this study were >85% cTnT+ ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... Pellet 1 was subjected to a second round of digestion in 1 mL of 1000 U/mL Collagenase type II and 1 mL of 11 U/mL Dispase II (Thermofisher, Cat# 17105041) along with the 8 mL of the remaining cell suspension ...
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bioRxiv - Immunology 2019Quote: ... 11-7139-42 or 85BRD, cat.: 11-7136-42), IL-4-PE (8D4-8, cat.: 12-7049-42) (all from Thermo Fisher Scientific); TNFa-Pacific Blue (MAb11 ...
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Cell culture dimensionality influences mesenchymal stem cell fate through cadherin-2 and cadherin-11bioRxiv - Cell Biology 2019Quote: ... Primary antibodies used were rabbit polyclonal anti-cadherin-11 and mouse monoclonal anti-cadherin-2 (3B9) (both 1:100; Thermo Fisher Scientific). Secondary antibodies used were goat anti-mouse Alexa Fluor 488 and goat anti-rabbit Alexa Fluor 647 (both 1:500 ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted using a modified phenol-chloroform method (11, 75) and quantified with the dsDNA High Sensitivity Assay kit (Thermo Fisher Scientific) on a Qubit 3.0 Fluorometer ...
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bioRxiv - Cancer Biology 2020Quote: ... MV4-11 (acute monocytic leukemia M5) and OCI AML3 (acute myelomonocytic leukemia M4) were maintained in RPMI 1640 (Gibco, Thermo Fisher Scientific) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... MV4-11 (acute monocytic leukemia M5) and OCI AML3 (acute myelomonocytic leukemia M4) were maintained in RPMI 1640 (Gibco, Thermo Fisher Scientific) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were digested with the filter-aided sample preparation (FASP) method23 and peptides were labeled using TMT 11-plex isobaric reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Flow cytometry analysis to measure parasitemia at the cycle of drug exposure was measured using the nucleic acid stain SYTO 11 (0.016 μM) (Life Technologies no. S7573) and a BD FACSCalibur machine as previously described (Rovira-Graells et al ...
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bioRxiv - Cancer Biology 2021Quote: ... For DHT stimulation experiments cells were starved of hormone for 48 hours in phenol red-free RPMI media (Gibco, 11-835-030) with 10% charcoal stripped FBS (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... The homogenate was washed HBSS four times and islets were handpicked into islet media-G11 {RPMI-1640 media with 11 mM glucose (Gibco, Dublin, Ireland) plus 10% heat-inactivated Fetal Bovine serum [HI-FBS] (Atlanta Biologicals ...
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bioRxiv - Neuroscience 2020Quote: ... and then the stylet was detached at the proximal end using a scalpel (Feather disposable scalpel, No. 11, Fisher Scientific #FH/CX7281A). The severed end was immediately placed in the silicone lubricant with the stylet tip facing the center of the coverslip ...
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bioRxiv - Cell Biology 2020Quote: ... and HeLa FlpIn Cells (Etemad, Kuijt, & Kops, 2015) cells were grown in a high glucose DMEM (Thermo Fisher Scientific 11-965-118/Gibco 11965118) supplemented with anti/anti (Thermo Fisher Scientific 15240062 ...
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bioRxiv - Microbiology 2022Quote: ... 11 mL cultures were grown at 37°C without shaking (HeraTherm™General Protocol Microbiological Incubator, Thermo Fisher Scientific, Waltham, MA, USA) in Balch tubes with N2/CO2 (80/20 ...
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bioRxiv - Pathology 2022Quote: ... Northern blotting was conducted as our previously reported method [66] with biotin-labeled DNA probes using Biotin-11-dUPT (Thermo Scientific USA). High-molecular weight RNAs were separated in 1 % formaldehyde agarose gels and transferred to Hybond-N+ membranes (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: For one sample of selected RGCs two dissected retinas were dissociated as previously described 58,11 and stained with CD90.2(Thy-1.2)-FITC antibody (Thermo Fisher REF#11-0902-81). Briefly ...