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Citations for Thermo Fisher :
351 - 400 of 619 citations for Elabela 19 32 TFA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... peptides were resuspended in 0.1 % TFA and loaded on 50 cm Easy Spray PepMap column (75 µm inner diameter, 2 µm particle size, Thermo Fisher Scientific) equipped with an integrated electrospray emitter ...
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bioRxiv - Biochemistry 2023Quote: ... Dried samples were redissolved with 300 μL of 0.1% TFA in H2O and further fractionated using high-pH reversed-phase peptide fractionation kits (ThermoFisher, P/N 84868) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... and the residual TFA salts were removed using a 7,000 MWCO Slide-A-Lyzer MINI Dialysis Unit (Thermo Scientific, Cat. No. 69562). The proteins were adjusted to 0.4 mg/mL in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Genetics 2024Quote: ... The digested and derivatized histone peptides were diluted in 0.1% TFA and injected to nLC-QqQ MS (Dionex nanoLC and a ThermoFisher Scientific TSQ Quantum). Peptides were first loaded to a trapping column (2 cm × 150 μm ...
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bioRxiv - Biochemistry 2024Quote: ... Dried samples were redissolved with 300 μL of 0.1% TFA in H2O and further fractionated using high-pH reversed-phase peptide fractionation kits (ThermoFisher, P/N 84868) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2019Quote: ... The lysate was centrifuged (20 min, 19 000g) and incubated with 2 ml of Ni-IMAC beads (ThermoFisher) per 1 l of culture on a rotatory wheel (1 h ...
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bioRxiv - Biochemistry 2020Quote: ... 19-805) of human ACE2 (GenBank NM_021804.1) was cloned in to the NheI-XhoI sites of pCEP4 (Invitrogen) with a N-terminal HA leader (MKTIIALSYIFCLVFA) ...
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bioRxiv - Neuroscience 2020Quote: ... RNA from the DIV 19 neuronal cultures were collected by using PureLink RNA Mini Kit (Ambion, Cat. # 12183018A). Quality and purity of RNA were confirmed by NanoDrop2000 ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mL 50% erythrocytes were transferred to 4 mL glass autosampler tubes (Thermo Fisher Scientific, 03-391-19), closed with screw caps with septa (Thermo Fisher Scientific 03-391-21) ...
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bioRxiv - Biochemistry 2022Quote: ... low passage ARPE-19 cells were electroporated (1400 V, 20 ms, 2 pulses, Neon Transfection System, Life Technologies) with a CRISPR/Cas9 ribonucleoprotein (RNP ...
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bioRxiv - Developmental Biology 2022Quote: ARPE-19 (ATCC Cat# CRL-2302) cells were authenticated by STR analysis and cultured in DMEM:F12 medium (Gibco) containing 10% FBS in a humid CO2 incubator described previously12 ...
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bioRxiv - Biochemistry 2020Quote: cDNAs encoding residues 19-143 of WT and R47H TREM2 were ordered as linear DNA strings (Life Technologies) and inserted into pHLSec ...
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bioRxiv - Neuroscience 2021Quote: ... CD IGS rat hippocampus (embryonic day 18-19) were quickly dissected and digested in 0.25% trypsin-EDTA (Invitrogen) at 37°C for 8-10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... CD IGS rat hippocampus (embryonic day 18-19) were quickly dissected and digested in 0.25% trypsin-EDTA (Invitrogen) at 37°C for 8-10 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Remaining GDP-loaded Rac1 and Cdc42 were then incubated with 500 µM GTPγS (ThermoFisher kit Cat# 16118/19) in the presence or absence of 2 nM of recombinant ERK3 protein (aa 9-327 ...
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bioRxiv - Genetics 2021Quote: ... 884 Y-SNPs targeted by the amplicons of the Ralf et al [19] AmpliSeq panel (Thermo Fisher Scientific) were included in the assay ...
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bioRxiv - Genomics 2021Quote: ... Human retinal pigment epithelial ARPE-19 cells (ATCC® CRL-2302™) were cultivated in DMEM/F12 (Gibco) with 10% fetal bovine serum (Lonza) ...
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bioRxiv - Immunology 2022Quote: ... Quantitative PCR was performed with the TaqPath™ COVID□19 CE□IVD RT□PCR Kit (Thermo Fisher Scientific) to obtain mean cycle threshold (Ct ...
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bioRxiv - Neuroscience 2023Quote: ... The antibody against TH phosphorylated at serine 19 (AB5935, used at 1:1,000) was obtained from Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... Amplification was performed on a 3×32 well Proflex PCR system (Applied Biosystems; Waltham, MA, USA). Reactions were amplified with a multi-step thermocycler protocol ...
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bioRxiv - Cell Biology 2020Quote: Ribosome footprinting was performed as described previously 32,with the use of RNase T1 (Thermo Scientific) instead of RNase I for the footprinting step ...
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bioRxiv - Systems Biology 2021Quote: ... Probes (10 pmol each) were labeled with [γ-32-P]-ATP using T4 PNK (Thermo Fisher) and hybridized to the membrane one by one in hybridization buffer (4× Denhardts solution ...
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bioRxiv - Systems Biology 2020Quote: ... PlatE cells (32) grown in 10 cm dish were transiently transfected using lipofectamine 2000 (Life Technologies) according to manufacturer’s instructions with retroviral vectors pMIG or pMIG-Sin1 ...
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bioRxiv - Neuroscience 2021Quote: ... and dopamine was detected at 32°C using an ECD-3000RS electrochemical detector (Thermo Fisher Scientific) set at a potential of 250 mV against an Ag/AgCl reference electrode ...
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bioRxiv - Immunology 2020Quote: ... All stains were carried out in media containing anti-CD16/32 blocking antibody (clone 93, ThermoFisher). For intracellular staining ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with anti-CD16/32 (Fc Block) antibodies and Fixable Live/Dead stain (Invitrogen) for 15 min before staining with antibodies for surface markers in 2% BSA/PBS buffer for 30 mins on ice ...
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bioRxiv - Physiology 2020Quote: ... USA) and connected to the 1/32” ID 3350 Tygon tubing (Cat: 025871A, Fisher Scientific, USA) with a 1/16” straight polypropylene barbed fitting (Cat ...
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bioRxiv - Cancer Biology 2019Quote: ... -HCC1937 and IMR-32 cell lines were cultured in RPMI (ATCC-modified vision, Thermo Fisher Scientific). MCF10A cells were cultured as described previously (Myant et al. ...
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bioRxiv - Biophysics 2023Quote: ... 32 μM each of dGTP/biotin-14-dATP/biotin-11-dUTP/biotin-14-dCTP (Thermo Fisher), and 5 U Klenow in 1× NEBuffer 2 (New England BioLabs ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in plating medium composed of DMEM/F:12 (ThermoFisher Scientific, Cat. No. 11330-32) supplemented with 10% FCS and penicillin/streptomycin.
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bioRxiv - Microbiology 2023Quote: ... Overnight cultures were grown in 18 × 150 mm glass culture tubes (Fisher Scientific 14-961-32). For overnight cultures of E ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were loaded with Fura-2 AM at 1 μg/mL (108964-32-5, Life Technologies) in HBSS or Fluo-4 AM at 10 μM in (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... xanthus cells were grown at 32°C in 1% CTT (1% (w/v) Bacto Casitone (Gibco) in TPM buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... and immunoblotted using primary antibody to Claudin-2 (1:500 dilution; Thermo Fisher Scientific, 32-5600) and α-Tubulin (1:1500 dilution ...
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bioRxiv - Immunology 2019Quote: ... Peptides dissolved in 0.1% TFA were pre–concentrated on a C18 trap column (Acclaim PepMap 100; 100 μm × 2 cm, 5 μm, 100 Å; Thermo Fisher Scientific) within 7 minutes at a flow rate of 30 μl/min with 0.1% TFA ...
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bioRxiv - Cancer Biology 2022Quote: ... Peptides dissolved in 0.1% TFA were pre-concentrated on a C18 trap column (Acclaim PepMap 100; 100 μm × 2 cm, 5 μm, 100 Å; Thermo Fisher Scientific) within 7 min at a flow rate of 30 μl/min with 0.1% TFA ...
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bioRxiv - Microbiology 2019Quote: ... HPLC far UV grade)/water (milliQ filtrated on 0.22 μm) + 0.05% trifluoroacetic acid (TFA, Sequencing grade; Thermo Fisher Scientific, San Jose, CA, USA), ratio (from 0 to 100% of acetonitrile during 80 min ...
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bioRxiv - Immunology 2020Quote: ... Peptide samples were loaded with 1% ACN/ 0.05% TFA on a 75 µm x 2 cm Acclaim™ PepMap™100 C18 Nanotrap column (Thermo Fisher Scientific) at a flow rate of 4 µl/min for 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Trapping was performed at 20 μL/min for 2 min in loading solvent A (0.1% TFA in water/ACN (99.5:0.5, [v/v]) on a 5 mm trapping column (Thermo Fisher Scientific, 300 μm internal diameter). After flushing from the trapping column ...
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bioRxiv - Systems Biology 2024Quote: ... 0.1% TFA in 3.5% ACN) was directly loaded onto a reversed-phase pre-column (Acclaim PepMap 100, Thermo Fisher Scientific, Waltham, United States) and eluted in backflush mode ...
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bioRxiv - Molecular Biology 2020Quote: ... HCoV-19 S protein was expressed in Sf9 insect cells using the Bac-to-Bac system (Life Technologies Inc.) and harvested from cell culture medium followed by purification procedure using Ni-NTA column and Superdex 200 gel filtration column (GE Healthcare ...
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bioRxiv - Bioengineering 2022Quote: ... the culture was moved to a pre-reduced 50 mL conical tube (Fisher Scientific, Cat. No. 06-443-19) on ice ...
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bioRxiv - Cell Biology 2022Quote: RNA was isolated from ARPE-19 cells by Trizol isolation and genomic DNA was subsequently removed with DNAseI (Invitrogen). cDNA was then generated from 1 μg of RNA using RevertAid First Strand cDNA Synthesis Kit (Thermofisher ...
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bioRxiv - Microbiology 2020Quote: We performed a multiplex RT-qPCR assay using the TaqPath RT-PCR COVID-19 kit (Thermo Fisher CN A47814) together with the TaqPath 1-step master mix – No ROX (Thermo Fisher CN A28523) ...
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bioRxiv - Cell Biology 2021Quote: ... ARPE-19 cells (ATCC) were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12) (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: FoxP3RBR-forkhead (19 μl, 0.25 mg/ml) was mixed with 20x Protein thermal shift dye (1 μl) (Thermo Scientific) in SEC buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genetics 2022Quote: ... ARPE-19 cells were transfected with these mRNAs using the Invitrogen Lipofectamine MessengerMAX Transfection Reagent (Fisher Scientific, Cat #: LMRNA001) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... RNAs from both treated and untreated arms of the experiment were separated on a 5% denaturing acrylamide:bisacrylamide (19:1) gel alongside a RiboRuler Low Range RNA Ladder (ThermoFisher) and the gel fixed ...
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bioRxiv - Bioengineering 2020Quote: ... gondii GT1 T NCBI Genome assembly version 7/19/2013 genome references with ERCC sequences (obtained from ThermoFisher website). The raw fastq files from sequencing are aligned to the concatenated genomes with STAR aligner (version 2.6.0c ...
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bioRxiv - Neuroscience 2021Quote: ... Coronal sections (15 μm) were cut with a cryostat at −19°C (Thermo Shandon Cryotome E, Thermo Scientific, USA) and collected on gelatin-coated slides for subsequent histochemical staining ...