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Citations for Thermo Fisher :
451 - 500 of 619 citations for Elabela 19 32 TFA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... RNA extraction and viral load analysis was performed as described previously [32] using the KingFisher Flex (Thermo Fisher) and the TaqMan RNA-to-CT 1-Step Kit (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... the device was filled with the bacteria suspension introduced using 1/32” Tygon tubing (15137044, Fisher Scientific, Switzerland) connected to a 1mL syringe (300-35-986 ...
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bioRxiv - Cell Biology 2020Quote: ... acetylated α-tubulin (Cell Signaling, #5335T Rabbit at 1:2000 and Invitrogen, #32-2700 Mouse at 1:2000), TOM20 (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... A silicon probe (Neuronexus A1×32-Poly2-5mm-50s-177-A32) covered in DiI solution (1,1’-dioctadecyl-3,3,3’,3’-tetramethyl indocarbocyanine; Invitrogen; UK) was implanted by lowering it slowly into the brain ...
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bioRxiv - Cell Biology 2020Quote: ... anti- HA-tag for KRAB protein (1:500, 32-6700 NOVEX HA epitope Tag antibody 5B1D10, Invitrogen, USA) and anti-b-Catenin (1:400 ...
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bioRxiv - Immunology 2022Quote: ... PTBP1-RNA complexes were precipitated with 2 µg of anti-PTBP1 antibody (ThermoFisher clone 1 cat#:32-4800) and 20 µl of Protein A/G beads (Pierce cat#:88802 ...
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bioRxiv - Immunology 2022Quote: ... 002-13 and 034-32 were collected on a 300 kV Titan Krios transmission electron microscope (ThermoFisher Scientific) equipped with Gatan K3 direct electron detector behind 30 eV slit width energy filter ...
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bioRxiv - Biochemistry 2022Quote: ... Primary cilia were visualized by staining with anti-acetylated alpha tubulin (1:400, Invitrogen, catalog no. 32-2700) and imaged using a Zeiss Axioplan microcope equipped with a Leica DMC6200 camera ...
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bioRxiv - Cell Biology 2022Quote: ... followed by hybridisation with a 32-P labelled TAA(CCCTAA)4 probe using UltraHyb-Oligo solution (ThermoFisher #AM8663) at 42 °C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... 32 channels probe (Neuronexus) or a NN1620 optotrod (for optogenetic manipulation – Neuronexus) were covered with DiI (Molecular Probes) and lowered into the MEC (depth 1482.42±186.16µm n=33 mice).
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bioRxiv - Immunology 2023Quote: ... and stained for 30mins at 4°C with: Fc blocker CD16/32 (clone 93, Invitrogen #14-0161-85), Epcam-BV421 (clone G8.8 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with polyclonal rabbit anti-βENaC (32) and monoclonal mouse anti-acetylated α-tubulin (Thermo Fisher Scientific Cat# 32-2700 ...
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bioRxiv - Cancer Biology 2023Quote: ... dissociated PDTOs were incubated with 150 μg/mL of mouse anti-human HLA-ABC (clone W6/32, ThermoFisher) to block MHC-I and 30 μg/mL mouse anti-human HLA-DR/DP/DQ (clone Tu39 ...
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bioRxiv - Cell Biology 2019Quote: ... 3-19 antibody was detected with IgG1 (γ1)-specific secondary antibody conjugated to Alexa Fluor 488 (Thermo Fisher Scientific, A-21121;), and 9G3 was detected with IgG2a (γ2a ...
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bioRxiv - Cell Biology 2020Quote: ... The air-dried DNA was dissolved in 19 µL of sterile water and treated with Swa1 in two cycles (2×2 FastDigest units, Thermo Scientific), each cycle of 1 hour at 37°C (total volume 25 µl ...
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bioRxiv - Immunology 2022Quote: ... with accession ID GSE190001 and BioProject ID: PRJNA785113 PREDICT-19 Cohort: Total RNA was isolated from whole blood lysate using the Tempus Spin Isolation kit (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: iPSCs (DF19-19-9-11T) purchased from WiCell Technologies were maintained on vitronectin-coated plates in Essential 8 medium (E8, ThermoFisher Scientific), and passaged when the colonies reached ~75-80% confluence ...
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bioRxiv - Biochemistry 2021Quote: ... R15A-mCherry wildtype or mutant expression vector and 950 ng of BFP marked empty or Actin (WT or E334R) expression vector (1:19 ratio of R15::Actin) using Lipofectamine LTX (Life Technologies) at a ratio of 3 μl Lipofectamine LTX and 1 μl of Plus reagent per ng of DNA in following complex formation in OPT-MEM according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cytotoxicity was measured by quantifying lactate dehydrogenase (LDH) from ARPE-19 (Pierce LDH Cytotoxicity Assay Kit, ThermoFisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions [12,20].
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bioRxiv - Microbiology 2021Quote: ... that had been previously labeled with Alexa-488 (anti-serotype 4-Alexa-488, to stain TIGR4) or Alexa-555 (anti-serogroup 19-Alexa-555, to stain GA47281) (Molecular Probes). Stained preparations were finally washed two times with PBS ...
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bioRxiv - Neuroscience 2022Quote: Cultured neurons at 19-21 DIV were incubated with 3 μM of the calcium dye Fluo-4 AM (Thermo Scientific, Sweden) in DMSO(Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: The cDNA coding for SARS CoV-2 Nsp1 1-180 Nsp2 1-19 8His was synthesised by GeneArt (Thermo Fisher Scientific) adding NcoI and NotI sites at the 5’ and 3’ ends ...
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bioRxiv - Cell Biology 2019Quote: Cytokine concentration of basal and apical supernatants of treated and untreated ARPE-19 cells were determined according to the protocol of a custom ProcartaPlex® multiplex immunoassay kit (ThermoFisher). Complement components in the cellular supernatant were quantified using the MILLIPLEX MAP Human Complement Panel (Merck) ...
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bioRxiv - Molecular Biology 2019Quote: ... 10ul RNA samples from lysate and from sucrose gradient fractions 1-19 were vacuum dried and dissolved in 10 ul RNA sample loading buffer (Invitrogen AM8552). RNAs were resolved by electrophoresis through 1.2%-agarose MOPS 6% formaldehyde gel for 4 h at 50 volts ...
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bioRxiv - Cell Biology 2021Quote: ... day 7 and day 19 samples all cells were adherent and collected using 2 ml/well accutase (Thermo Fisher Scientific, A1110501) and a cell scraper ...
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bioRxiv - Microbiology 2020Quote: ... and pcDNA3.1 SARS-CoV-2 spikeΔ19 plasmid (encoding SARS-CoV-2 spike protein, with 19 AA truncated in C terminal) using Lipofectamine 3000 (Invitrogen, L3000-015) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was quantified using real the Genesig® Real Time PCR Coronavirus COVID-19 (CE IVD) kit (Primer Design) using the manufacturer’s protocol and QuantStudio 5 Real-Time PCR System thermocycler (Thermo Fisher Scientific)
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bioRxiv - Immunology 2021Quote: ... MN908947) C-terminally truncated by 19 amino acids was cloned into a derivative of the pCDNA3.1 expression vector (Thermo Fisher Scientific). Substitutions and deletions in the Spike protein gene open reading frame (shown in Figure 1 ...
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bioRxiv - Bioengineering 2022Quote: ... combining 1 μL sample with 19 μL reaction mixture in a Nunc 384-well non-treated flat-bottom white microplate (Thermo Fisher). Luminescence spectra (413 – 563 nm ...
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bioRxiv - Microbiology 2022Quote: All samples were tested by quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) with either the TaqPath COVID-19 Combo Kit from Thermofisher (MHome) or CDC Influenza SARS-CoV-2 Multiplex Assay (HIVE) ...
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bioRxiv - Neuroscience 2022Quote: ... HPCs were seeded at passage 19 at a density of 13,200 cells/well in NUNC™ 96-well plates (ThermoFisher, #167008). Cells were treated with 1% participant serum 24 hours post-seeding for the proliferation assay ...
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bioRxiv - Microbiology 2022Quote: ... 12 and 19 post-hatch from Salmonella-infected chicks as described above and quantified using Qubit dsDNA fluorometric assay (Thermo Fisher). DNA quality was assessed by DNA ScreenTape assay using TapeStation 2200 (Agilent) ...
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bioRxiv - Cell Biology 2022Quote: ... reactions were supplemented with 50 mM MgCl2 and 500 µM GDP (Active Rac1/Cdc42 pull-down kit, ThermoFisher, Cat# 16118/19) and incubated for 15 min at 37°C with gentle agitation ...
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bioRxiv - Cell Biology 2022Quote: Levels of GTP-bound Rac1 and Cdc42 were determined using either active Rac1/Cdc42 pull-down and detection kit (Cat# 16118/19, ThermoFisher Scientific) following the of the manufacturer’s instructions and buffer composition or purified GST-Pak1-PBD fusion beads ...
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bioRxiv - Microbiology 2023Quote: Flow cytometry was carried out according to standard methods as described in [19] using an Attune NxT flow cytometer (ThermoFisher Scientific). Cells were detached with non-enzymatic cell dissociation solution (Merck ...
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bioRxiv - Immunology 2023Quote: ... followed by C-terminal 8x His and 2x Strep tags for affinity purification.[19] The trimeric Spike protein was expressed as previously reported.[71–73] transiently expressed in suspension-adapted ExpiCHO cells (Thermo Fisher) in ProCHO5 medium (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation (19 000 rpm for 45 min at 4°C in a Fiberlite F21-8×50y (Thermo Scientific). Supernatant was incubated with 5 ml Glutathione Sepharose 4B beads slurry (Cytiva ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... with the adjustment that reactions were quenched with 200μl of ice-cold 100% methanol (n=3).19 Formation of TFV-DP was detected by mass spectrometry as previously described using a Dionex Ultimate 3000 uHPLC (Thermo Fisher) coupled to a TSQ Vantage triple quadrupole mass spectrometer (Thermo Fisher).19 Column temperature was maintained at 40°C ...
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bioRxiv - Neuroscience 2023Quote: ... repair template (Supplemental Table 1) were introduced into ARPE-19 cells using electroporation (1400 V, 20 ms, 2 pulses, Neon Transfection System, Life Technologies). After electroporation ...
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bioRxiv - Biochemistry 2023Quote: ... 24 hours later cells were transfected with 2 ug DNA (mCherry tagged PPP1R15A DNA and empty carrier DNA at a ratio of 1:19) using the lipofectamine LTX system (Life Technologies) at a ratio of 3 μl lipofectamine LTX to 1 μl Plus reagent for every nanogram of DNA in 200 μl of Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: Tilt series data of purified cores were acquired using SerialEM software [19] on a Titan Krios G4 transmission electron microscope (Thermo Scientific), operated at 300kV and equipped with e-CFEG ...
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bioRxiv - Microbiology 2020Quote: ... and cells were grown for six days at 32 °C according to the manufacturer’s High Titer protocol (Thermo Fisher). Supernatants were then passed through a 0.22 µm filter and batch-purified using IMAC resin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Intestinal samples were fixed in paraformaldehyde (PFA 32%, Fischer Scientific) 4% in phosphate buffer saline (PBS, Gibco, Waltham, MA) for 1 hour at 20°C ...
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bioRxiv - Neuroscience 2019Quote: ... IMR-32 neuroblastoma cells (ATCC Cat# CCL-127) were maintained in DMEM/Ham’s F12 (1:1) with 15mM HEPES (Invitrogen) supplemented with 10% FBS and 100 U/ml penicillin/ 100 µg/ml streptomycin ...
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bioRxiv - Microbiology 2019Quote: ... and baby hamster kidney 21 cells expressing the MHV receptor (BHK-R) (32) were maintained at 37°C in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% serum (HyClone FetalClone II ...
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bioRxiv - Biochemistry 2021Quote: The HegG2 hepatoma cell line (32) was maintained in Dulbecco’s Modified Eagle’s Medium (DMEM 25mM high glucose, 21969-035, Gibco, Life Technologies), 4mM glutamine ...
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bioRxiv - Microbiology 2021Quote: ... and 1 μl of a 1:100 dilution (corresponding to 4.7 aM of ERCC-0016) of RNA spike-in mix 1 (External RNA Control Consortium (32) (Ambion)) was added before amplification was performed to monitor the technical performance of the assay showing linear amplification of specific probes (Fig ...
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bioRxiv - Immunology 2022Quote: ... Axenic amastigotes were generated by culturing stationary-phase promastigotes at 32°C in Grace’s insect cell culture medium (Invitrogen), pH 5.2 ...
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bioRxiv - Microbiology 2020Quote: ... After 24 hours (24-32 hpi) parasite DNA was stained with SYBR Green DNA stain (1: 10,000) (Invitrogen #S7563) for 30 min at 37 °C and the fluorescence intensity was measured using a MACS Quant Analyzer 10 (at least 200,000 RBCs were measured per sample ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted as described [32] and the RNA concentration quantified using a Nanodrop 2000 spectrophotometer (Thermo Scientific). RNA quality was analyzed using an Agilent Bioanalyzer 2100 ...