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Citations for Thermo Fisher :
301 - 350 of 829 citations for Ebola Virus Secreted Glycoprotein sGP Bundibugyo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific) and real-time PCR was performed using TaqMan assays (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Cellular RNA was mixed with TaqMan Fast Virus 1-step Master Mix (Applied Biosystems), 10 mM forward and reverse primers and 2 mM probe ...
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bioRxiv - Biochemistry 2022Quote: HIV-1 virus-like particles (VLPs) were produced by Lipofectamine 3000 (Thermo Fisher Scientific) transfection of 293FT cells (purchased from Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Strep-tag (NBP2-41073; Novusbio) or influenza virus hemagglutinin tag (2-2.2.14; Thermo Scientific). After washing with PBS with 1% BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... Yamanaka reprogramming factors were induced to iPSCs via Sendai virus using CytoTune2.0 (ThermoFisher, A16517). To establish nGFP+ iPSCs ...
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bioRxiv - Genetics 2023Quote: ... Virus particles were subsequently washed multiple times with PBS (pH 7.4, Thermo Fisher Scientific) and physical titers were measured using a Qubit 3.0 fluorometer and the Qubit dsDNA HS assay kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Virus pellets and cell lysates were dissolved in radioimmunoprecipitation assay (RIPA) buffer (Thermo Scientific) containing protease inhibitors ...
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bioRxiv - Microbiology 2023Quote: ... containing 5 µl of template and TaqMan Fast Virus 1-step mastermix (Applied Biosystems). Primer sequences and concentrations and thermal cycling conditions for SARS-CoV-2 nucleocapsid 1 gene were as previously described (13) ...
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bioRxiv - Immunology 2023Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region and gene-specific probes and reverse primers as follows ...
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bioRxiv - Developmental Biology 2023Quote: IPS cells were derived from skin fibroblasts using Cytotune Sendai virus kit (Thermofisher, France) and cultured on MEF in KO- DMEM supplemented with KO-SR (Thermofisher ...
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bioRxiv - Neuroscience 2023Quote: ... reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific). Real-time PCR was performed using TaqMan assays (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 or 3 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2023Quote: ... Abelson virus-transformed pre-B cells were maintained in DMEM (Thermo Fisher, 11960-077) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... along with psPAX2 and pMD2.G packaging and envelope virus using lipofecctamine 3000 (Invitrogen) in serum-free Opti-MEM media (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region and N gene-specific probe and reverse primer as previously described47:
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bioRxiv - Cell Biology 2024Quote: Elephas maximus endothelial and epithelial cells were transduced with Sendai virus (Thermofisher Scientific, A16517) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... of Iowa) was prepared by diluting virus stocks to 400 p.f.u./µL into OptiMEM (Gibco) followed by addition of CaCl2 to a final concentration of 10 µM ...
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bioRxiv - Microbiology 2020Quote: ... and transfected for virus reconstitution into BHK-21 cells with Lipofectamine 3000 (ThermoFisher #L3000-075).
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed using the TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems). Assays were conducted on a QuantStudio5 Q-PCR machine (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... The assay was performed using the TaqMan® Fast Virus 1-Step Kit (Life Technologies) and kit manual ...
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bioRxiv - Molecular Biology 2020Quote: ... a 20μL monoplex reaction contained 5μL of 4x TaqMan Fast Virus Master Mix (Thermo Fisher), 0.6µL of each primer (0.3μM final concentration each) ...
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bioRxiv - Immunology 2019Quote: ... two-fold virus stock dilutions were prepared in U-shaped 96-well plates (Thermo Scientific). The same volume of a 1% human O+ red blood cell suspension (Innovative Research ...
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bioRxiv - Microbiology 2019Quote: ... Other antibodies were obtained from commercial vendors as follows: Anti-coxsackie virus B3 antibodies (ThermoFisher), Anti-adenovirus A5 antibodies (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... RAW264.7 cells were then transduced with virus and selected using hygromycin at 10mg/ml (Invitrogen) to select for cells containing the shRNA plasmid.
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bioRxiv - Microbiology 2021Quote: ... and the number of virus particles was determined using a Nanodrop 2000 (Thermo Fisher Scientific). The adenovirus stocks were aliquoted and stored at -80°C.
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bioRxiv - Neuroscience 2021Quote: ... using TaqMan Fast Virus 1-Step Master Mix (PN4453800, Applied biosystems, Thermo Fisher Scientific, USA). PrimerTime primers and FAM-dye coupled detection probes were used for detecting Clstn3 mRNA level ...
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bioRxiv - Neuroscience 2021Quote: ... The final buffer for the virus was: dPBS + 5% Glycerol + 0.001 Pluronic F-68 (Invitrogen). The AAV titre (1.71 × 1013 GC/ml ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqManTM Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... The qPCR for Sendai virus detection was performed using TaqMan Gene Expression Assays (Life Technologies). Quantitative RT-PCR was performed using SYBR® Green PCR Master Mix (Applied Biosystems™ ...
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bioRxiv - Cell Biology 2022Quote: Cell lines were generated using Sendai virus CytoTune-iPS 2.0 Sendai reprogramming kit (Thermo Fisher) for transfection of Yamanaka’s factors ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL of purified virus was analyzed with the Quant-it PicoGreen dsDNA Assay (Invitrogen).
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bioRxiv - Biochemistry 2022Quote: ... concentrated virus was run on a 4-20% Tris-Gly SDS-PAGE gel (Life Technologies), which was then analyzed using the Pierce Silver Stain Kit (Pierce ...
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bioRxiv - Immunology 2022Quote: ... For these assays we used TaqMan Fast Virus 1- Step Master Mix (ThermoFisher, catalog # 4444432) and custom primers/probes targeting the E gene sgRNA (forward primer ...
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bioRxiv - Molecular Biology 2022Quote: Viral RNA was extracted from amplified virus stock using Trizol reagent (Invitrogen; Cat no. #15596018). Reverse transcription was carried out using in-house produced Moloney murine leukemia virus (MMLV ...
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bioRxiv - Neuroscience 2021Quote: ... using TaqMan Fast Virus 1-Step Master Mix (PN4453800, Applied biosystems, Thermo Fisher Scientific, USA). PrimerTime primers and FAM-dye coupled detection probes were used for detecting Clstn3 mRNA level ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA synthesis was performed using Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV, Invitrogen). PCR was performed in an AriaMx thermal cycler (Agilent ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... UKEi1-A was reprogrammed with a Sendai Virus strategy (CytoTune iPS Sendai Reprogramming Kit, ThermoFisher). These studies were approved by the Ethical Committee of the University Medical Center Hamburg-Eppendorf (Az ...
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bioRxiv - Immunology 2022Quote: ... RNA from virus-infected cells was isolated using PureLink RNA Mini Kit (Thermo Fisher Scientific), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... genomic DNA was extracted from 1010 virus using the PureLinkTM Genomic DNA mini kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The virus was dialyzed with PBS supplemented with 0.05% of Pluronic F68 (Thermo Fisher Scientific) using 100-kDa filters (Millipore) ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus (MMLV) reverse transcriptase (#28025013, Ambion) and a mixture of anchored oligo-dT and random decamers (IDT) ...
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bioRxiv - Plant Biology 2019Quote: ... 10 IU of Moloney murine leukemia virus RNase H+ reverse transcriptase solution (Thermo Fisher Scientific), and appropriate buffer containing deoxynucleoside triphosphates (dNTPs ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated total RNA was reverse transcribed with Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen) and oligo-dT primers ...
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bioRxiv - Microbiology 2021Quote: ... MRC-University of Glasgow Centre for Virus Research) were maintained in Leibovitz’s L15 media (Gibco) containing 10% FBS (Gibco) ...
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bioRxiv - Microbiology 2020Quote: Compound dilutions for virus treatment were performed in Minimum Essential Media (MEM) purchased by Gibco.
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bioRxiv - Microbiology 2019Quote: ... and transfected for virus reconstitution into BHK-21 cells with Lipofectamine 3000 (ThermoFisher #L3000-075). HSV-1 expressing the FLAG-tagged N-terminal extension of UL54 virus were reconstituted and titrated on Vero 2-2 cells50 ...
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bioRxiv - Microbiology 2019Quote: Concentrated virus aliquots were mixed with DiI (final concentration of 1 μM, Thermo Fisher, USA), vortexed and incubated for 1.5 h at room temperature (RT) ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR reactions were performed using TaqMan® Fast Virus 1-Step Master Mix (ThermoFisher) and 5 µl of isolated RNA as a template to detect a 132 bp sequence in the ORF1b/NSP14 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... expanded PBMCs were transduced with non-integrating Sendai reprogramming virus (Cytotune 2.0, Thermo Fisher, A16517) according to manufacturer’s instructions added to the Expansion Media ...
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bioRxiv - Genomics 2020Quote: ... endometrium tissues fibroblast cells were transfected with a Sendai virus reprogramming kit (Life Technologies, A16517). The transfected cells were then plated onto Matrigel-coated culture dishes according to the manufacturer’s instructions ...