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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Dengue Virus serotype 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Recombinant virus was made by co-transfection into SF9 insect cells (Invitrogen) of the plasmid and BacVector3000 baculovirus DNA (Novagen ...
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bioRxiv - Systems Biology 2019Quote: ... and MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies), and the reaction was carried according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... and MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies), and the reaction was carried according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The virus samples were treated with 300 units of RNase I (Ambion) for 30 min followed by SUPERaseI RNase Inhibitor (Thermo Fisher ...
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bioRxiv - Cancer Biology 2019Quote: ... 2.5mL virus with 10μg/mL polybrene (Thermo Fisher Scientific TR-1003-G) was added to 5×105 cells plated in 6-well dishes ...
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bioRxiv - Microbiology 2020Quote: ... The virus was rescued and propagated in T-REx-293 cells (Invitrogen). Purification was by CsCl gradient ultracentrifugation ...
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bioRxiv - Cancer Biology 2020Quote: ... Concentrated virus was filtered using 0.45um syringe filters (ThermoFisher; Cat#: 725-2545) and immediately added to cultured OSE in 24-well plates ...
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bioRxiv - Microbiology 2020Quote: ... Stocks of virus were grown in Vero-E6 cells in DMEM (Gibco) supplemented with 10% fetal bovine serum (Atlas Biologicals ...
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bioRxiv - Microbiology 2020Quote: ... containing 7,5 μl 4xTaqMan Fast Virus 1-Step Master Mix (Life Technologies) and 1 μl Primer/Probe mixture29,30 ...
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bioRxiv - Microbiology 2020Quote: ... the virus particles were resuspended in CO2-independent media (ThermoFisher, Cat# 18045088), aliquoted ...
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bioRxiv - Immunology 2019Quote: ... purified virus was immobilized on 96-well maxisorp microtiter plates overnight (Nunc). Wells were blocked with 0.05% PBST [0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... c-MYC and KLF4 in CytoTune Sendai virus vectors (Thermo Fisher Scientific) (Sontag et al. ...
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bioRxiv - Microbiology 2021Quote: ... The virus was rescued and propagated in T-REx-293 cells (Invitrogen). Purification was by CsCl gradient ultracentrifugation ...
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bioRxiv - Immunology 2021Quote: ... Vero cells were used to propagate the virus in DMEM medium (Gibco) supplemented with 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... Virus was removed from cells and replaced with post-infection media (Gibco OptiMem supplemented with 0.01% FBS ...
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bioRxiv - Biophysics 2022Quote: ... X-31 influenza virus was diluted in Opti-MEM (Gibco, cat # 11058021) at a ratio of 1:10000 to add 250 µL into each well (25) ...
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bioRxiv - Microbiology 2022Quote: ... the reactions with Taqman Fast Virus 1-step master mix (ThermoFisher scientific) were half of the recommended 20 μl volume ...
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bioRxiv - Neuroscience 2023Quote: ... The virus-containing pellet was resuspended in 200 μL of DPBS (ThermoFisher) and stored at -80 °C ...
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bioRxiv - Microbiology 2023Quote: ... 10-fold dilutions of each virus in DMEM + 1% antibiotic/antimycotic (Gibco) were added to a monolayer Vero E6-TMPRSS2-T2A-ACE2 cells for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis was performed with Moloney murine leukemia virus reverse transcriptase (Invitrogen) at 38°C for 60 min ...
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bioRxiv - Microbiology 2023Quote: ... Each virus was mixed with PBS-washed defibrinated sheep blood (Fisher Scientific) containing 5 mM ATP to a concentration of 106 PFU/mL and fed to ∼50-60 female mosquitoes ...
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bioRxiv - Genomics 2022Quote: ... The precleared nuclear lysates (2 mg) were incubated with purified biotinylated RNA in NT2 buffer along with Yeast tRNA (20 µg/ml; Thermo Fisher Scientific #AM7119) with gentle rotation for 1.5 hours at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Lysate equivalent to 2×106 cells per well was run on a NuPAGE 4 to 12% Bis-Tris gel (Thermo Fisher Scientific, USA). Western blots were performed on samples and membranes were probed with anti-PTPN22 (clone D6D1H ...
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bioRxiv - Physiology 2020Quote: ... The supernatant was transferred to a chilled microcentrifuge tube and protein concentration was determined using 2 μl of lysate in a Bicinchoninic Acid (BCA) Assay according to manufacturer’s instructions (Thermo Scientific, Waltham, MA, USA). To standardize the amount of input material in downstream assays ...
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bioRxiv - Microbiology 2023Quote: ... An equivalent of 2×107 parasites whole protein lysate was loaded on a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen, cat log # NP0321BOX) followed by transfer onto a PVDF membrane (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... lysates were partially digested with RNAseT1 (Ambion) (0.25U per mg of lysate 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... Lysate was treated with DNase (DNAfree, Ambion) and diluted with 400 μl of correction buffer (NP-40 ...
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bioRxiv - Neuroscience 2021Quote: ... Lysate was clarified by centrifugation (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... lysates were digested with RNaseI (Invitrogen, #AM2294), then layered onto a 1 M sucrose cushion in Footprinting Buffer plus 0.5 mM DTT and centrifuged in a Beckman Optima TLX Ultracentrifuge at 60,000 rpm for 1 hour at 4°C using a TLA100.3 rotor to isolate 80S ribosomes ...
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bioRxiv - Neuroscience 2020Quote: ... Lysate and sample buffer (Life Technologies, NP0008) were boiled ...
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bioRxiv - Cancer Biology 2023Quote: ... and lysates were treated with RNAseI (Ambion) before ultracentrifugation over a sucrose gradient using an SW-41Ti rotor at 222,200 x g for 2 hours at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... supernatant was harvested for virus titration and cells were lysed in Trizol (Invitrogen). MHV in the supernatant was quantified by standard plaque assay on L2 cells.
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bioRxiv - Cell Biology 2020Quote: ... HEK-293T cells used for virus production were cultured in IMDM (Life Technologies) supplemented with 20% IFS ...
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bioRxiv - Neuroscience 2020Quote: ... Virus-containing supernatant was harvested three days after transfection with Lipofectamine 2000 (Thermofisher). This supernatant was then cleared from cell debris by centrifugation at 3500 rpm for 15 min and filtered through a 0.45 µm filter (Millipore) ...
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bioRxiv - Neuroscience 2021Quote: ... to wash the virus in Opti-MEM™ Medium (ThermoFisher Scientific, Darmstadt, Germany) and concentrate to a final volume of 150 μl.
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bioRxiv - Neuroscience 2020Quote: ... They were reprogrammed into iPSCs using the replication incompetent Sendaï virus kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The virus pellet was then re-suspended in PBS (Thermo Fisher Scientific, 10010015) containing 25 mM HEPES buffer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... For this the virus was diluted in 3 mL of 199 medium (Gibco). After incubation for 2 h at 37°C the medium was aspirated and 3 mL of fresh DMEM (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... respectively) and 5 μl of 4X TaqMan Fast Virus 1-step mastermix (ThermoFisher). The qPCR plate was sealed and read in the FAM and HEX channels using a QuantStudio™ 5 Real-Time PCR system (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... using the Taqman Fast Virus 1-stepMaster Mix enzyme (Thermo Fisher, Waltham, MA). The limit of detection of this assay is estimated to be 200 genome equivalents/ml saliva or swab fluid ...
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bioRxiv - Neuroscience 2020Quote: ... Cellular reprogramming experiments were conducted using the Sendai virus (CytoTune, Gibco, Life Technologies) carrying the four factors Oct4 ...
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bioRxiv - Neuroscience 2020Quote: ... Cellular reprogramming experiments were conducted using the Sendai virus (CytoTune, Gibco, Life Technologies) carrying the four factors Oct4 ...
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bioRxiv - Microbiology 2021Quote: ... or Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 1X TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific; 4444434) for a total reaction volume of 20 μl in 96-well PCR plates (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion) and a mixture of anchored oligo-dT and random decamers (IDT) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) in a total reaction volume of 10 μl ...
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Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... ten-fold dilutions of virus were prepared in diluent medium (Opti-MEM (Invitrogen) with 2% FBS (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... The virus was rescued and propagated in T-Rex 293 HEK cells (Invitrogen) which repress antigen expression during virus propagation ...
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bioRxiv - Immunology 2021Quote: ... human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the SARS-VoV-2 S protein were co-transfected into 293T cells (ATCC) ...
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bioRxiv - Genomics 2021Quote: ... hiPSCs were generated from primary fibroblasts using Sendai virus reprogramming kit (ThermoFisher Scientific), yielding 2-3 different clonal lines for each individual ...