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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Dengue Virus serotype 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight with either Dengue 2 fixed virus antigen (Microbix, Cat# EL-22-02) or polyvalent total immunoglobulin (Invitrogen, Cat# H17000). Before adding PBMC’s ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA from either wild type dengue plasmid or mutated dengue plasmid were quantified using a High Sensitivity Range Qubit (Thermo Fisher). Library preparation was carried out with the Nextera XT DNA Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... The dengue 2 E protein antibody PA5-32246 was used at 1:10,000 for western blotting (Thermo-Fisher Scientific, Waltham, MA). Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL ...
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bioRxiv - Microbiology 2023Quote: ... Virus pellets and cell lysates were dissolved in radioimmunoprecipitation assay (RIPA) buffer (Thermo Scientific) containing protease inhibitors ...
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bioRxiv - Microbiology 2022Quote: ... pneumoniae serotype 2 strain D39 [17] was plated on brain heart infusion (BHI, Thermo Scientific, MA, USA) agar plates supplemented with 3% v/v defibrinated horse blood (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: RNA was isolated from virus-containing medium or cell lysates using TRIzol LS (ThermoFisher Scientific, #10296010) according to the manufacturer’s instructions and stored at −80°C for RT-qPCR ...
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bioRxiv - Immunology 2021Quote: ... Dengue viruses were grown in C6/36 cells cultured in RPMI 1640 (Gibco 11875-093), L-Glutamine (Gibco 25030-081) ...
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bioRxiv - Immunology 2021Quote: ... Virus particles in 2% FBS/DMEM/1mM Pen/Strep (Thermofisher, 15140122) were incubated with cells for 2 hours at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The cell and virus lysates were Western blotted and probed with a goat polyclonal anti-gp120 antibody (Invitrogen), the 4E10 anti-gp41 antibody (NIH HIV Reagent Program ...
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription and quantitative PCR (RT-qPCR) was performed using 0.25 µl of lysate and Taqman Fast Virus 1-step Master Mix (Applied Biosystems) on a CFX384 thermal cycler (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... Lysates were placed on a DynaMag-2 magnet (ThermoFisher Scientific) for 1 minute after which supernatant was discarded ...
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bioRxiv - Microbiology 2023Quote: ... Strep-tag (NBP2-41073; Novusbio) or influenza virus hemagglutinin tag (2-2.2.14; Thermo Scientific). After washing with PBS with 1% BSA ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were treated with 2 U/μl of RNase I (Ambion) for 45 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was incubated with 2 mL glutathione-sepharose (Fisher Scientific) for 1 hr and washed with 50 mL (25 CV ...
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bioRxiv - Microbiology 2020Quote: ... 24 h later the dish was washed with PBS and treated with PR8 virus (MOI =2) in the infection medium [F-12K with 2% BSA fraction V (Thermo Fisher), 1% antibiotic-antimycotic (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: Protein (2 µg total lysate, 5 µg soluble lysate) was loaded onto 4-12% Bis-Tris NuPAGE minigels (Invitrogen, NP0322BOX). 10 µL of precision plus protein kaleidoscope prestained standard (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Concentrated virus stocks in reduced-serum DMEM (2% FBS) supplemented with 50mM HEPES buffer (Gibco #15630080) were stored at -80°C ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were treated with 2 units of Turbo DNase (Life Technologies) in 10 mM MgCl2 for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... approximately 2 mg of lysate and 25uL of Protein G Dynabeads (ThermoFisher) were incubated with 1 μg of the indicated antibody or control IgG overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were treated with 2 units of Turbo DNase (Life Technologies) and 10 mM MgCl2 for 30 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... cells or virus pellet were incubated with 2 mM of EZ-Link Psoralen-PEG3-Biotin (ThermoFisher Scientific) at 37°C for 10 min in PBS containing 0.01% digitonin ...
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bioRxiv - Microbiology 2021Quote: The spike of the SARS-CoV-2 virus (D614G variant) was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the codon-optimized sequence obtained by Ou et al ...
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bioRxiv - Microbiology 2023Quote: ... The BA.1 and BA.2 supernatants were additionally concentrated using the Intact Virus Precipitation Reagent (Invitrogen). All viral stocks were stored at -80°C until use ...
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bioRxiv - Cell Biology 2023Quote: ... BacMam 2.0 virus (Invitrogen, C10593).
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bioRxiv - Molecular Biology 2021Quote: ... 300 μg lysates were pre-cleared with 2 μl Protein A Dynabeads (Invitrogen) for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were mixed with 2×Native Tris-Glycine sample buffer (LC2673, Life Technologies) and loaded directly into a precast 10% polyacrylamide gel (4561034 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were collected in 2 ml of RIPA buffer (Thermo Scientific, 89900) containing a protease inhibitor cocktail (cOmplete™ ...
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bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated for 2 hours with the virus and then washed twice with Neurobasal-A medium (Gibco) containing 2% B-27 (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... 12 µL of SARS-CoV-2 stock virus (USA_WA1/2020; 8.3×104 pfu) in OptiMEM supplemented with 1x antibiotic/antimycotic (Gibco) was pipetted directly onto each surface being tested and spread with a pipette tip to facilitate efficient drying ...
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bioRxiv - Microbiology 2021Quote: ... The spike of the SARS-CoV-2.SctΔ19 B.1.617.2 (delta) virus was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the full protein sequence of the original SARS-CoV-2 isolate Wuhan-Hu-1 (WH1 ...
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bioRxiv - Microbiology 2023Quote: ... l ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for l h ...
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bioRxiv - Microbiology 2024Quote: ... and 2 U/µl of M-MulV-RT (Reverse transcriptase of Moloney Murine Leukemia Virus; Thermo Scientific, USA).
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bioRxiv - Immunology 2019Quote: ... Oligonucleotide primers and a dual labelled probe for DENV 1,2,3,4 serotypes were used (Life technologies, USA) based on published sequences ...
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bioRxiv - Immunology 2020Quote: ... Oligonucleotide primers and a dual labeled probe for DENV 1,2,3,4 serotypes were used (Life technologies, India) based on published sequences [19].
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bioRxiv - Molecular Biology 2020Quote: ... Lysate equivalent to 1-2 × 105 cells was resolved by SDS-PAGE (Life Technologies) and transferred to nitrocellulose membranes (Amersham) ...
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bioRxiv - Cancer Biology 2020Quote: ... Each lysate was pre-incubated with 2 μg IgG and 20 μl Dynabeads (Invitrogen) for 4 h at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Lysate was then incubated with 2 μL of α-V5 (IgG isotype control; Invitrogen) or 11 μL of α-Orc2 (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2023Quote: ... Sheared lysates were pre-cleared using 2 µg rabbit IgG antibody (Thermo Fisher Scientific) per sample for 1 hr at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... virus with Trypan Blue (Gibco, 1µl) was injected into the lateral ventricle using glass pipette (WPI) ...
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bioRxiv - Immunology 2022Quote: ... Virus was diluted in OptiMem (ThermoFisher) and applied apically at a MOI 0.1 for two hours at 34,5°C with 5% CO2.
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bioRxiv - Bioengineering 2023Quote: ... Varicella zoster virus solution (Fisher Scientific) was dropped onto a No.1 coverslip and dried on top ...
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bioRxiv - Cell Biology 2023Quote: Cytotune OSKM Sendai virus (A16517, Invitrogen) and EmGFP reporter Sendai Virus (A16519 ...
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bioRxiv - Microbiology 2021Quote: ... was propagated on Vero E6 cells at a multiplicity of infection (MOI) of 0.001 in virus diluent (DMEM supplemented with 2% FBS, 1X Penicillin/Streptomycin (Gibco), 1mM sodium pyruvate (Gibco ...