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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Dengue Virus Subtype 4 Envelope 45 kDa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was filtered through an 0.45 µm filter and loaded onto a pre-equilibrated 5 ml His-Trap HP column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... 4–12% Bis-Tris Protein Gels (Thermo Fisher Scientific) under reducing conditions ...
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bioRxiv - Cancer Biology 2021Quote: ... or 4-12% gradient NuPAGE Protein Gel (Thermo Fisher). Proteins were transferred to Newblot PVDF membranes (Mandel Scientific) ...
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bioRxiv - Pathology 2021Quote: ... and 10 µg/mL fluorescently labeled 40 kDa and 150 kDa dextrans (Invitrogen) were added on top ...
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bioRxiv - Immunology 2024Quote: ... The lentiviral envelope vector pLP/VSVG was purchased from Invitrogen (Invitrogen/ life technologies) and the packaging vector psPAX2 was a kind gift from Didier Trono (Addgene plasmid # 12260).
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bioRxiv - Cell Biology 2021Quote: ... anti-PDHA (1:1000, Invitrogen #45-6600), anti-p-Akt (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... BAPTA75 (30μM; Invitrogen, #B1204, 45 min incubation) directly dissolved in culture media and TTX76 (18μM ...
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bioRxiv - Immunology 2022Quote: ... anti-mouse CD8a (45-0081-82, Invitrogen), For intracellular cytokine staining ...
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bioRxiv - Cancer Biology 2020Quote: ... Thy1.1 PerCP-Cy5.5 (HIS51, ThermoFisher 45-090082), CD45 PE (30-F11 ...
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bioRxiv - Developmental Biology 2021Quote: ... + 45% F12 + GlutaMAX (Thermo Fisher, 31765-092) + 10% KnockOut serum replacement (Thermo Fisher ...
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bioRxiv - Pathology 2023Quote: ... for 45 minutes and tyramide-Alexa488 (Invitrogen) for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 45% F-12 (ThermoFisher Scientific, Waltham, MA), 10% CTS Knockout SR Xenofree Medium (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and 45 U/mL penicillin-streptomycin (Gibco). All cell lines were screened for mycoplasma at regular intervals by screening fixed cells for irregular DAPI stain ...
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bioRxiv - Neuroscience 2021Quote: ... The molecular weights were determined by using an appropriate pre-stained protein standard (HiMark 31-460 kDa, Invitrogen) for high molecular weight proteins.
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bioRxiv - Neuroscience 2021Quote: ... astrocyte conditioned media samples were concentrated 20x in 10 kDa cut-off centrifuge protein concentrators (Thermo Fisher 88517) to enrich for WT GPC4 before mixing with gel loading buffer ...
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bioRxiv - Genetics 2020Quote: ... we used PageRuler Plus Prestained Protein Ladder covering a range from 10 to 250 kDa (Thermo Fisher Scientific) and 2.5 nM pure pol ε purified as described [98] ...
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bioRxiv - Physiology 2022Quote: ... We loaded 10 μL of the PageRuler Plus Prestained Protein Ladder (10–250 kDa, Thermo Fisher, Waltham, MA) alongside the samples.
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bioRxiv - Biophysics 2023Quote: ... The protein was then buffer-exchanged into HBS using a 7 kDa Zeba desalting column (Thermo Fisher, 89882) and stored at -80 ºC until use.
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was measured spectrophotometrically (ε280 21430 M-1cm-1, MW 24.79 kDa) using NanoDrop One (Thermo Scientific). Dialysed MSP1D1 was concentrated to 5 mg/ml using a 10 kDa MWCO Amicon Ultra centrifugal concentrator (Millipore) ...
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bioRxiv - Cancer Biology 2023Quote: ... Vesicular stomatitis virus glycoprotein-pseudotyped virus was produced by co-transfecting 293T cells using Lipofectamine 2000 (Invitrogen) with an shRNA transducing vector and 2 packaging vectors ...
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bioRxiv - Biophysics 2020Quote: ... 20 mM MES pH 6.0) at 4 °C for 12 h using 3.5 kDa MWCO Slide-A-Lyzer MINI Dialysis Devices (Thermo Scientific) to remove imidazole and CHAPS ...
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bioRxiv - Physiology 2022Quote: ... Lungs were inflated with room air through the tracheal cannula and perfused through the pulmonary arterial and left atrial cannulas at 0.4-0.6 mL/min with autologous blood diluted in a solution of 4% dextran (70 kDa; Molecular Probes), 1% FBS (Gemini Bio-Products) ...
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bioRxiv - Immunology 2021Quote: ... 25 μl of virus was immediately mixed with 25 μl of serially diluted (2×) protein A/G purified IgG (ThermoFisher) from mouse sera (starting at 500 μg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... the proteins were separated with the NuPAGE system (ThermoFisher, 4-12% Bis-Tris protein gel), and transferred to PVDF membrane (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... 40μg of protein was run on NuPAGE 4-12% Bis-Tris protein gel (ThermoFisher Scientific) and transferred to PVDF membrane using the iBlot 2 system ...
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bioRxiv - Microbiology 2022Quote: ... Protein extracts were separated on 4-12% Bis-Tris mini protein gels (NuPage®, Invitrogen) and blotted on polyvinylidene fluoride membranes (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins (25 µg) were separated on Bolt™ 4-16% Bis-Tris Protein Gels (Invitrogen) and transferred onto nitrocellulose membrane via iBlot gel transfer stacks (Invitrogen) ...
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bioRxiv - Physiology 2021Quote: ... Proteins were separated on 4-12% Bis-Tris Protein Gels (NuPage Novex, Thermo Fisher Scientific) and transferred to nitrocellulose membrane (GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2021Quote: Protein samples were separated in NuPAGE 4-12% Bis-Tris protein gels (#NP0336BOX; ThermoFisher Scientific) and transferred to PVDF membrane (#LC2002 ...
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bioRxiv - Physiology 2023Quote: ... Mitochondrial proteins were thereafter separated by SDS–PAGE (4–12% Bis-Tris Protein Gels; Invitrogen) and transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated using a 4-12% Bis-Tris Protein SDS-PAGE Gel (Life Technologies). Samples were transferred to a nitrocellulose membrane (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 μg protein was loaded onto a 4-12% bis-tris gradient protein gel (Invitrogen) under reducing conditions and transferred onto PVDF membrane ...
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bioRxiv - Physiology 2024Quote: ... The denatured proteins (20 µg/well) were separated by 4-12 % protein gel (Thermo Scientific) and transferred onto PVDF membranes (MiliporeSigma ...
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bioRxiv - Physiology 2024Quote: ... Proteins were separated on 4-12% Bis-Tris Protein Gels (NuPage Novex, Thermo Fisher Scientific) and transferred to nitrocellulose membrane (GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins separated by NuPAGE™ 4-12% Bis-Tris precast protein gels (Thermo Fisher Scientific), and then transferred to nitrocellulose membrane (GE Healthcare) ...
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bioRxiv - Systems Biology 2022Quote: All induced pluripotent stem cell (iPSC) lines were reprogrammed by the Sendai virus expressing 4 Yamanaka factors (CytoTune®-iPS Sendai Reprogramming Kit, Invitrogen) as previously described36 ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuged at approximately 250 × g for 5 min at 4 °C and resuspended in thawed Geltrex™ LDEV ([lactose dehydrogenase elevating virus]-Free Reduced Growth Factor Basement Membrane Matrix; Gibco). Two 15-μl drops of the matrix-cell suspension were pipetted into wells of a Nunc™ 24-well-Nuclon Delta-treated plate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: We diluted freshly purified virus samples in DPBS and combined three volumes of the virus with one volume of NuPAGE LDS Sample Buffer (4×; Thermo Fisher) containing 100 mM dithiothreitol ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were cleared by centrifugation at 20,000 rpm for 45 min and the His-SUMO fusion proteins were enriched on Ni-NTA beads (Thermo Fisher Scientific). The protein was eluted using a buffer containing 25 mM Tris (pH 8.0) ...
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bioRxiv - Cell Biology 2022Quote: ... The beads were again separated using a magnetic rack and the entire volume was then loaded onto a precast 10-well 4-12% Bis-/Tris NuPAGE gel to separate the proteins for 45 min and 180 V in 1x MES buffer (Thermo Scientific).
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bioRxiv - Neuroscience 2019Quote: ... Fluorescein (10 kDa) or tetramethylrhodamine (70 kDa) dextrans (100 μM; Thermo Fisher; Waltham, MA), approximating the molecular weights of α-synuclein and Hsc70 ...
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bioRxiv - Cell Biology 2021Quote: ... TβRII-Fc (124 kDa) and murine mAb 1D11 (150 kDa) (Thermo Fisher, Waltham, MA) were each captured on individual flow cells to 100-140 RU ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed 3 times in warm PBS and fixed for 45 min at room temperature in 4% (w/v) formaldehyde (Thermo Scientific; 28908) in PBS containing 0.1% Triton-X100 ...
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bioRxiv - Neuroscience 2020Quote: ... a 50 μg Fluo-4 stock solution was diluted by 45 μL Pluronic F-127 20% solution in DMSO (P3000MP, Thermo Fisher Scientific) and added to the HEK293T cells in a 48-well plate to the final concentration of 4 μM ...
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bioRxiv - Microbiology 2020Quote: ... They were then spun down at 15,000 g for 45 minutes at 4°C and the supernatant was passed over a nickel resin column (Thermo Fisher Scientific, # PI88222) and eluted using buffer B (30mM K2HPO4 ...
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bioRxiv - Physiology 2023Quote: ... followed by separation for 45 min at 200 V in MOPS buffer in pre-cast 4 – 12% gradient Bis-Tris precast gels (Nupage, Thermo Fisher Scientific). This was followed by transferring onto nitrocellulose membranes (0.45-μm pore size ...
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bioRxiv - Microbiology 2022Quote: ... and intracellular markers were stained with monoclonal antibodies for 45 minutes at 4°C with FoxP3 (FJK-16S) and IL-10 (JES5-16E3) (All antibodies were from Thermo Fisher Scientific). Cells were resuspended in PBS with 2% NCS and 2 mM EDTA ...