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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Dengue Virus Subtype 4 Envelope 45 kDa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
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bioRxiv - Microbiology 2021Quote: ... A mouse monoclonal anti-HSV 45-kDa protein (scaffolding protein) antibody (Serotec) at 1:2000 dilution and Alexa Fluor 568–goat anti-mouse IgG (Invitrogen) at 1:1000 dilution were used as primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... along with psPAX2 and pMD2.G packaging and envelope virus using lipofecctamine 3000 (Invitrogen) in serum-free Opti-MEM media (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: Influenza A virus (IAV) strain A/WSN/33 [H1N1 subtype] was propagated in Madin-Darby Canine Kidney (MDCK) cells (ThermoFisher). Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Biophysics 2023Quote: ... and run in a Bio-Rad Mini-PROTEAN Tetra Vertical Electrophoresis Cell at 50 mA for approximately 45 minutes alongside a prestained protein ladder (PageRuler Prestained Protein Ladder, 10 to 180 kDa, Thermo Fisher Scientific). Gels were stained for 1 h in a Coomassie solution (0.1% Coomassie brilliant blue R-250 ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight with either Dengue 2 fixed virus antigen (Microbix, Cat# EL-22-02) or polyvalent total immunoglobulin (Invitrogen, Cat# H17000). Before adding PBMC’s ...
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bioRxiv - Neuroscience 2021Quote: ... and ovalbumin Alexa Fluor® 594 conjugate 45 kDa (O34783, Invitrogen) tracers were mixed at 1:1 ratio and infused at a concentration of 5 μg/μl ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA from either wild type dengue plasmid or mutated dengue plasmid were quantified using a High Sensitivity Range Qubit (Thermo Fisher). Library preparation was carried out with the Nextera XT DNA Library Prep kit (Illumina ...
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bioRxiv - Genetics 2022Quote: ... Standard curves for each antibody subtype (Invitrogen standards) were run with each plate for a specific subtype to determine antibody concentrations.
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... including Alexa 555-conjugated ovalbumin (45 kDa) and Fluorescein 3000 MW Anionic dextran (3 kDa) were purchased from Invitrogen (CA, USA). D-Amphetamine (14204 ...
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bioRxiv - Neuroscience 2023Quote: ... subtype H1N1 (A/California/04/2009) (A42579, Life Technologies), Coronavirus (COVID-19 Spike Protein ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein samples were resolved on 4-12% NuPAGE Bis-Tris gel with protein molecular weight standards (PageRuler Prestained Protein Ladder, 10 to 180 kDa, Thermofisher). The gels were transferred onto PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... virus was mixed with 4% fast green (Invitrogen) dissolved in saline and injected using a glass micropipette at a rate of 100 nl/min ...
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bioRxiv - Plant Biology 2021Quote: ... 4 ng GST or GST-VDAL purified protein were loaded onto 10 kDa microspin columns (Ambion). After washed twice with 1 mL HS buffer (50 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... Immune complexes were pulled down for 45 min at 4°C with 25 µL protein G dynabeads (Invitrogen), captured on a magnet and washed 4 times in 800 µL IP buffer ...
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bioRxiv - Physiology 2023Quote: ... Ovalbumin (OA) Alexa Fluor-488 conjugate (molecular weight 45 kDa) were obtained from Life Technologies Corporation ...
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bioRxiv - Neuroscience 2023Quote: ... or 45 kDa Texas Red-conjugated ovalbumin (Thermo Fisher Scientific, O23021; 1 mg ml-1) were diluted in saline and withdrawn via a connecting line into the cannula ...
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bioRxiv - Microbiology 2020Quote: ... The dengue 2 E protein antibody PA5-32246 was used at 1:10,000 for western blotting (Thermo-Fisher Scientific, Waltham, MA). Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were co-transfected with the pNL4.3.Luc.R-E-viral vector as well as a plasmid expressing the vesicular stomatitis virus glycoprotein (VSV-G) envelope (pMD2.G) using Lipofectamine 3000 (Life Technologies) according to basic manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Immobilised virus was fixed with 4% formaldehyde (Thermo Scientific) in phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... which were incubated overnight at 4°C with an anti-OxPhos antibody cocktail (Invitrogen, #45-8099, #45-7999), and were analyzed using the Western Breeze Chromogenic Western Blot Immunodetection Kit (Invitrogen).
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bioRxiv - Neuroscience 2019Quote: ... for 45 minutes or Fluo-4 AM (Invitrogen Cat# F14201) for 25 minutes at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... pEco envelope was used to produce virus to infect murine T cells) using lipofectamine 2000 (Cat. No. 11668019, Invitrogen, Carlsbad, California, USA) on a 100□mm2 poly-D-lysine–coated plate (Corning ...
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bioRxiv - Developmental Biology 2021Quote: ... at 4°C for 2 hours with gentle agitation and subsequently with 45 μL Dynabeads Protein G (Thermo Fisher Scientific) at 4°C overnight with gentle agitation ...
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bioRxiv - Developmental Biology 2022Quote: ... at 4°C for 2 hours with gentle agitation and subsequently with 45 μL Dynabeads Protein G (Thermo Fisher Scientific) at 4°C overnight with gentle agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by 45 min incubation with Protein G beads (Invitrogen). The complex was washed four times in ice cold PBS ...
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bioRxiv - Immunology 2021Quote: ... proteins with a molecular weight (MW) below 100 kDa were separated on 4-12% Bis/Tris gels (Life Technologies) in MES SDS Buffer (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Bacterial lysate was clarified by centrifugation at 40,000 xg for 45 min at 4°C and the clarified protein bound to glutathione agarose beads (Thermo Fisher Scientific) at 4 °C for 4 hours with rotation ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of the cell lysate protein and equal volume of the virus pellet protein were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris gel; Invitrogen/Thermo Scientific) and transferred onto nitrocellulose membrane by semidry transfer ...
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bioRxiv - Immunology 2022Quote: ... Rat anti mouse antibodies specific for IgG subtypes (IgG1, IgG2b, IgG2C& IgG3-Invitrogen) (Ref ...
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bioRxiv - Developmental Biology 2022Quote: ... Alexa Fluor-conjugated anti-mouse IgG subtypes/rabbit/chicken secondary antibodies (Molecular Probes) were diluted 1:200 in blocking solution before use ...
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bioRxiv - Developmental Biology 2019Quote: ... staining was performed with Alexa Fluor 488nm or 633nm subtype-specific secondaries (Invitrogen) at 1:200 ...
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bioRxiv - Immunology 2023Quote: ... plasma cell subtypes were cultured in RPMI1640 (Thermo Fisher Scientific, Waltham, MA, USA) supplemented 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of the cell lysate protein and equal volume of the virus pellet protein were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris gel; Invitrogen/Thermo Scientific) and transferred onto nitrocellulose membrane by semidry transfer ...
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bioRxiv - Immunology 2021Quote: ... Dengue viruses were grown in C6/36 cells cultured in RPMI 1640 (Gibco 11875-093), L-Glutamine (Gibco 25030-081) ...
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bioRxiv - Cell Biology 2020Quote: ... and pre-stained protein ladder 10-250 kDa (Thermo Scientific). CIZ1-contaning fractions were pooled and reduced glutathione removed by buffer-exchange (50 mM Tris-HCl pH 7.0 at 25°C ...
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bioRxiv - Neuroscience 2021Quote: ... PageRuler pre-stained protein ladder 10-180 kDa (ThermoFisher, #26616) was used as the molecular size marker.
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bioRxiv - Developmental Biology 2021Quote: ... The virus was spun at 500 g for 5 min and the supernatant filtered through a 45 μm polyethersulfone filter (Fisher Scientific) and subsequently concentrated ...
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bioRxiv - Developmental Biology 2020Quote: ... subtype-specific secondary antibodies coupled to either AlexaFluor 488 or 555 were used (Invitrogen). AlexaFluor 405 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 10 to 30 μg of total protein was loaded onto NuPAGE Bis-Tris 4-12% acrylamide gels (Thermo Fischer Scientific) along with prestained protein ladder PageRuler (10-180 kDa) (Thermo Fisher Scientific) and run at 100 V ...
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bioRxiv - Microbiology 2022Quote: ... then 7 µg of protein along with PageRuler Prestained ladder 10 kDa to 180 kDa (26616, Thermo Fisher Scientific) was loaded on a NuPage 4-12% gradient Bis-Tris pre-cast SDS-PAGE gel (NP0321 ...
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bioRxiv - Immunology 2021Quote: ... The lentiviral envelope vector pLP/VSVG (Invitrogen) and the packaging vector psPAX2 (Trono lab ...
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bioRxiv - Molecular Biology 2020Quote: ... and envelope plasmid in OptiMEM (Life Technologies) for 5 to 6 hours (PEI:total pDNA μg ratio 2:1) ...
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bioRxiv - Microbiology 2022Quote: ... and the concentration of total virus proteins were determined by a BCA protein assay kit (Thermo Fisher) (31 ...
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bioRxiv - Plant Biology 2021Quote: ... the PageRuler™ Prestained Protein Ladder (10 to 180 kDa, ThermoFisher) was used ...
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bioRxiv - Molecular Biology 2024Quote: ... PageRuler Plus™ Prestained Protein Ladder (Thermo Fisher, 10 – 250 kDa) was resolved as a molecular weight reference ...
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bioRxiv - Cell Biology 2024Quote: ... A molecular weight protein marker (10–460 kDa; HiMark #LC5699, Invitrogen) was used for MW analyzes.
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bioRxiv - Neuroscience 2021Quote: ... HEK293 cell expressed and binds ACE2) and ovalbumin Alexa Fluor-488 conjugate (Cat# 034781; molecular weight 45 kDa) were obtained from Life Technologies Corporation ...
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bioRxiv - Neuroscience 2023Quote: ... The specific primers for different T cell subtypes were designed Primer Express software (Applied Biosystems) at exon junctions to avoid genomic DNA amplification ...
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bioRxiv - Neuroscience 2024Quote: ... incubated overnight at 4 °C with the OxPhos Complexes antibody cocktail (Invitrogen #45-8099), diluted 1:1250 in blocking solution ...