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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat ...
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bioRxiv - Immunology 2023Quote: ... TaqMan Fast Virus 1-step Master Mix (Thermo Fisher Scientific), primers ...
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bioRxiv - Neuroscience 2023Quote: ... and Moloney murine leukemia virus (M-MLV) Reverse Transcriptase (Invitrogen). One microgram of total RNA was retrotranscribed and the cDNA was diluted 1:10 to the quantitative PCR (qPCR) ...
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bioRxiv - Immunology 2024Quote: ... Virus inoculum was prepared in DMEM (Thermo Fisher Scientific, 119905040) supplemented with 2% FBS and allowed for 1 hour at 37°C for virus adsorption onto cells ...
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bioRxiv - Immunology 2024Quote: ... Antibodies used: anti-Influenza A virus NS1 (PA5-32243, ThermoFisher), anti-acetyl-α-tubulin (Ly640 ...
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bioRxiv - Microbiology 2022Quote: ... envelope plasmid (0.9 µg pMD2.G) and pLX304 carrying eGFP-LC3B (9 µg) using Opti-MEM (Fisher Scientific) and FuGENE HD transfection reagent (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... 6ug of psPAX2 viral packaging vector and 3ug pMD2.G viral envelope vector overnight with Lipofectamine 3000 (Invitrogen) according to manufacturer protocol ...
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bioRxiv - Genetics 2022Quote: ... The Sf-9 cell line was obtained from Sigma and maintained as adherent culture in the insect serum free SF900 II SFM (Thermo Fisher Scientific) medium supplemented with 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2019Quote: ... tissues were incubated in 10 µM EdU diluted in insect media (HyClone SFX; Thermo Fisher Scientific) for the indicated amount of time ...
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bioRxiv - Biochemistry 2022Quote: The midguts dissected from the insects for microscopy were placed in L-15 culture media (Invitrogen) supplemented with 5% (v/v ...
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bioRxiv - Immunology 2024Quote: ... and SB28 RRV-IRF8 (100% transduced) cells were plated at 1x105 cells (n=3 per time point) and counted (Thermofisher Countess 3)at 24 and 48 hours post-seeding ...
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bioRxiv - Cell Biology 2020Quote: ... the plate was checked for confluence and the cells were infected with serial dilution of virus in OptiPro serum free media (Thermofisher) with 1% L-Glutamin (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times with PBS to remove residual input virus and overlaid with DMEM with glutamine (Life Technologies) with 0.7% IgG-free BSA (Sigma) ...
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bioRxiv - Bioengineering 2021Quote: ... Untransduced cells and cells treated with the serially diluted virus were both cultured in the presence of 2 µg/ml puromycin (Invitrogen). The lentiviral titer was quantified by counting the number of living cells at the time when all of the untransduced cells died as previously described26.
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bioRxiv - Neuroscience 2021Quote: ... Cell reprogramming was performed using the Sendai virus system with SOX2/OCT4/KLF4/MYC (CytoTune-iPS Reprogramming Kit; Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... VeroE6 cells were used for live virus titration of biological samples and were maintained in DMEM (#11965092, Thermo Scientific, USA) with 10% FBS.
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bioRxiv - Cell Biology 2022Quote: ... 4T1 cells were transduced with cell-free virus-containing supernatants for 48 hours and selected with 100 µg/mL of neomycin (Gibco) for 96 hours.
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bioRxiv - Immunology 2021Quote: ... dilutions of IgG were co-incubated with the virus (1000-2000 TCID50) for 1 h at 37°C prior to addition of VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
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bioRxiv - Immunology 2020Quote: ... Cells and virus were incubated for 4 hours at 37°C/5% CO2 and washed with PBS (Gibco, Waltham, MA) to remove excess virus ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were validated free of virus mycoplasma contamination using the MycoSEQ assay (Thermo Fisher Scientific, Waltham, MA, USA) or STAT-Myco assay (IDEXX Bioanalytics) ...
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bioRxiv - Microbiology 2022Quote: Blood virus mixtures were titrated by ten-fold serial dilution in virus media (RPMI 1640 cell culture media supplemented with 5% fetal bovine serum [FBS] and 1% Penicillin-Streptomycin [Gibco]) in a 96-well plate before transferring dilutions to equivalent wells of a 96 well plate containing near confluent C6/36 cell monolayers ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Virus propagation and titration was performed in LLC-MK2 cells using Opti-MEM supplemented with 1.6% TrypLE™ Express (Gibco) and 100 μg/ml CaCl2 (OptiMEMinf ...
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bioRxiv - Microbiology 2023Quote: BmN cells infected with WT virus at indicated time were harvested and total RNA was extracted by using TRIzol reagent (Invitrogen). 2 μg total RNA was prepared to synthesis the cDNA by TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen) ...
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bioRxiv - Microbiology 2023Quote: ... The following day we washed cells with DPBS twice and added half-log10 dilutions of the virus sample in infection media (MEM medium (Gibco) containing 0.3% BSA (fraction V ...
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bioRxiv - Microbiology 2023Quote: Influenza A virus (IAV) strain A/WSN/33 [H1N1 subtype] was propagated in Madin-Darby Canine Kidney (MDCK) cells (ThermoFisher). Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Genomics 2024Quote: ... the fibroblasts were thawed and plated at a density of 2.5×105 cells/well of 6-well plate and infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
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bioRxiv - Cell Biology 2024Quote: ... GH3 cells were transduced with supernatants containing virus carrying the shMeCP2 or shhnRNP L construct using the Lipofectamine 3000 reagent (Invitrogen). After 48 h ...
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bioRxiv - Cancer Biology 2023Quote: Cell surface proteins were isolated by using Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific, cat#89881), following the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and PC-3 cells were cultured in RPMI1640 (Gibco) with 10% FBS and 1% Antibiotic-Antimycotic ...
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bioRxiv - Biophysics 2021Quote: ... counted using a Countess 3 FL cell counter (Invitrogen), centrifuged for 2 min at 200 g ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with 3 mg/ml puromycin (Invitrogen) for an additional 3-5 days to select for cells that had integrated the viral DNA ...
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bioRxiv - Microbiology 2020Quote: ... Calu-3 cells were cultured in Advanced DMEM (Gibco) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... SK-OV-3 cells were cultured in McCoy’s (Gibco) medium supplemented with 100units/mL penicillin ...
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bioRxiv - Neuroscience 2019Quote: ... cells were treated with Rhod-3/AM (ThermoFisher; R10145) for 30 minutes at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... Calu-3 cells (ATCC) were maintained in MEM (Gibco) supplemented with 10% v/v foetal calf serum (FCS ...
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bioRxiv - Microbiology 2022Quote: Infected Calu-3 cells were harvested in Trizol (Thermofisher) 24h post infection and total RNA extracted according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... staining and the Countess 3 automatic cell counter (Invitrogen). BMDMs wells were combined and incubated in RIPA buffer (25mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... Cell viability was measured in triplicates using the CellTiter-GloR Luminescence assays from Promega or by counting live cells using Countess 3 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cell count was determined using a Countess 3 (Invitrogen). Neutrophils were seeded onto 12mm glass coverslips (Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... counted on a Countess 3 automated cell counter (Invitrogen) and reseeded at 200 000 cells or 80 000 cells per well when kept for two or three days respectively.
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bioRxiv - Molecular Biology 2023Quote: ... cell numbers were determined by Countless 3 (Thermo Fisher). Cell pellet was resuspended in 300-600 μL acidic extraction buffer (0.3 M HCl ...
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bioRxiv - Genomics 2023Quote: ... Viable cells were counted using a Countess 3 (Invitrogen) counter and trypan blue (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... UW228-3 cells were cultured in RPMI (ThermoFisher, 61870044) and D425 cells in DMEM/F12+Glutamax ...
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bioRxiv - Cancer Biology 2024Quote: ... HG-3 cells were electroporated using either Neon (Invitrogen) in the case of RNP or the Celetrix LE+ system (Celetrix ...
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bioRxiv - Genomics 2024Quote: The Invitrogen Countess Automated Cell Counter 3 (Thermo Fisher) was used to count cells ...
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bioRxiv - Microbiology 2024Quote: Calu-3 cells were grown in DMEM (Gibco, 41966029) supplemented with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... Western blots were performed with NuPAGE Novex 3– 8% Tris-Acetate Protein Gels (Life Technologies).
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bioRxiv - Biophysics 2022Quote: ... Protein was liquated to 3 replicate wells of a 384-well–plate (Thermo Scientific Nunc), and plates were incubated at 37 °C for 60 h with shaking ...
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bioRxiv - Biophysics 2022Quote: ... Protein was liquated to 3 replicate wells of a 384-well–plate (Thermo Scientific Nunc), and plates were incubated at 37 °C for 60 h with shaking ...
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bioRxiv - Molecular Biology 2021Quote: ... 10% glycerol) were loaded on 3-12% gradient Bis-Tris NativePAGETM precast protein gels (Invitrogen) and subjected to BN- PAGE electrophoresis as described previously (75 ...