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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with virus containing scrambled control or targeting MTR and Polybrene (8 ug/mL, Invitrogen). Cells were split after 48 h into standard RPMI-media (10% FBS ...
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bioRxiv - Genomics 2021Quote: ... cells or virus pellet were incubated with 2 mM of EZ-Link Psoralen-PEG3-Biotin (ThermoFisher Scientific) at 37°C for 10 min in PBS containing 0.01% digitonin ...
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bioRxiv - Biochemistry 2021Quote: ... high titer virus generation (V1) was then used to infect 1200 ml High Five™ cells (Invitrogen) for large scale protein production ...
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bioRxiv - Immunology 2020Quote: ... Virus stocks used in vivo were produced by two passages on Vero E6 cells in DMEM (Gibco) without FBS ...
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bioRxiv - Microbiology 2021Quote: ... Wild-type mumps virus (MuVi/Utrecht.NLD/40.10; genotype G) was grown on Vero cells in DMEM (Gibco) with 2% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Plasmid transfection and virus infection: Expression constructs were transfected into cells using Lipofectamine 2000 Transfection Reagent (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293T cells (ATCC) were cultured for virus production in Dulbecco’s Modified Eagle Medium: Nutrient Mixture (DMEM) (GIBCO) supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Microbiology 2023Quote: ... Infectious virus was reconstituted by transfecting ARPE-19 cells with BAC DNA and Lipofectamine 3000 (ThermoFisher L3000001); high titer stocks generated after three passages in ARPE-19 cells were used for this project ...
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bioRxiv - Microbiology 2023Quote: ... infected cells were stained with primary antibody (Vaccinia Virus Polyclonal FITC Antibody ThermoFisher PA1-73191, 1:1000) and Hoechst 33342 (1:10,000) ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed twice with PBS and then incubated with virus suspended in infectious medium (DMEM; Invitrogen) supplemented with 35% bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2023Quote: ... This virus mixture was used to infect 1.8–2.4 liters of High Five cells (Thermo Fisher Scientific) at a cell density of 3 × 106 per ml for 60 h at 27°C ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293T cells used for the production of virus were cultured in DMEM supplemented with 2mM glutamine (Gibco), 10% Tetracycline-free FBS (Sigma-Aldrich ...
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... cells were infected with virus diluted in 10 mL of Opti-MEM (ThermoFisher Scientific, Waltham, MA, USA) and incubated for 1 h at 37°C to allow for virus adsorption ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: The wild-type copy of the xopX gene and its 14-3-3 protein binding motif mutants were cloned in the yeast two-hybrid vector pDEST32 (Invitrogen) using the Gateway cloning system (Invitrogen ...
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bioRxiv - Bioengineering 2019Quote: ... cells were fixed with 3% paraformaldehyde (ThermoFisher) in PBS for 20min at room temperature and subsequently washed three times with PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-3 L of Expi293F cells (ThermoFisher) were grown at 37°C to a cell density of 3.2×106 cells/mL in FreeStyle expression medium (ThermoFisher) ...
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bioRxiv - Neuroscience 2021Quote: ... protein was extracted from 3-week mature neurons using neuronal protein extraction reagent (N-PER) (Fisher Scientific, Waltham, MA) and protease inhibitor cocktail (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... 2010) as follows: protein samples were separated in NuPAGE™ 3-8% Tris-Acetate Protein Gels (Thermo Fisher EA03755) in Tris-Acetate-EDTA running buffer (Thermo Fisher BP13354 ...
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bioRxiv - Developmental Biology 2022Quote: ... 20μg of each respective sample was loaded onto a 1mm 3-8% Tris-Acetate Mini Protein gel (NuPAGE) along with 10μl of Spectra Multicolor High Range protein ladder (Thermofisher). The gel was run at 80V for 15 minutes and then increased to 120V until the dye front reached the bottom ...
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bioRxiv - Plant Biology 2019Quote: ... About 20 µg of total soluble proteins of each sample was loaded to 3-12% Bis-Tris 1.0 mm Invitrogen NativePAGETM protein gels from ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The lentiviruses were obtained by transient transfection of the psPAX2 packaging plasmid and pMD2.G envelope plasmid in HEK293T cells by using Lipofectamine 3000 Transfection Reagent (Thermo Fisher Scientific, Waltham, MA, USA). Transfection efficiency was validated by western blotting ...
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bioRxiv - Neuroscience 2019Quote: ... fly brains or imaginal discs were promptly dissected in Schneider’s insect medium (Life-Gibco), properly mounted and scanned ...
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bioRxiv - Molecular Biology 2019Quote: ... larval brains were dissected and placed in insect media (HyClone SFX; Thermo Fisher Scientific) supplemented with 0.1 mM colchicine for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... RNA extraction from individual insects included homogenization with 1 mL of Trizol reagent (Ambion) and further purification using RNeasy kits (QIAGEN ...
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bioRxiv - Developmental Biology 2022Quote: ... Flies were dissected in Grace’s Insect Medium (Cat. No. 11605045; Invitrogen, Carlsbad, California, USA), and then fixed in 4% formaldehyde (Cat ...
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bioRxiv - Microbiology 2023Quote: ... Rasgon (The Pennsylvania State University) and were grown in Schneiderʹs insect medium (Gibco 21720024) supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: ... braziliensis parasites (strain MHOM/BR/01/BA788) were grown in Schneider’s insect medium (GIBCO) supplemented with 20% heat-inactivated FBS ...
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bioRxiv - Neuroscience 2019Quote: ... Patient KS1-1 was consented for stem cell derivation and cells were reprogrammed using non-integrating Sendai virus vectors (CytoTune-iPS 2.0, ThermoFisher Scientific, Waltham, MA). 5 days post-induction ...
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bioRxiv - Cell Biology 2021Quote: ... packaging plasmid psPAX2 (3μg) and envelope plasmid pMD2.G (3μg) using Lipofectamine 2000 Transfection Reagent (Invitrogen). Post 18 hr ...
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bioRxiv - Cancer Biology 2022Quote: ... pSPAX2 packaging vector and pVSVG envelope vector using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) as described (73) ...
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bioRxiv - Immunology 2021Quote: ... packaging plasmid (pCMV-dR8.2) and envelope plasmid (pCMV-VSV-G) using Lipofectamine-LTX reagent (Thermo Fisher Scientific India Pvt ...
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bioRxiv - Molecular Biology 2020Quote: ... at 1-3 mg/ml and Dynabeads® Protein G (Thermo Fisher Scientific) according to the manufacturer’s instruction.
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bioRxiv - Biochemistry 2021Quote: ... with 50ug of protein separated using 3-8% tris-acetate gels (Invitrogen EA0378) and transferred using an iBlot2 transfer system (Invitrogen IB21001) ...
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bioRxiv - Biophysics 2021Quote: ... and loaded into NativePAGE™ 3–12% Bis-Tris protein gels (ThermoFisher Scientific). BN-PAGE was performed using two different running buffers ...
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bioRxiv - Biochemistry 2022Quote: ... Protein complexes were separated on 3-12% NativePAGE Novex Bis-Tris gels (Invitrogen). Electrophoresis was performed for 30 min at 150 V followed by 2 hours at 250 V ...
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bioRxiv - Biochemistry 2023Quote: ... Native PAGE™ Novex® 3-12% Bis- Tris Protein Gels (Thermo Fisher) gels were loaded with 40 μg of mitochondrial proteins ...
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bioRxiv - Microbiology 2023Quote: ... for LapA or 3 ul Spectra Multicolor High Range Protein Ladder (Thermo Fisher) for MapA ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 × 10-4 M MTG and 5% Protein-Free Hybridoma Media II (Gibco).
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: The wild-type copy of xopX and its 14-3-3 protein binding motif mutants were cloned by Gateway cloning (Invitrogen, California) from the pENTR clones to the BiFC vector pDEST-VYCE(R)GW carrying the C-terminal region of the Venus Fluorescent Protein (VFP ...
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bioRxiv - Neuroscience 2023Quote: ... Lysates containing 60ug of total protein were electrophoresed for 3 hrs at 40C and 100 V using 3%-12% Bis-Tris polyacrylamide gels (ThermoFisher, BN1001BOX) and a running buffer containing 15mM Bis-Tris ...
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bioRxiv - Immunology 2019Quote: ... Ka539 cells transfected with a virus expressing luciferase reporter were cultured in DMEM/high glucose and IMDM (Gibco) supplemented with 10% FBS (Natocor) ...
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bioRxiv - Microbiology 2020Quote: ... The cell layers were fixed on-chip and subjected to immunofluorescence microscopic analysis for influenza virus NP (Invitrogen) and neutrophils (CD45 ...
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bioRxiv - Microbiology 2020Quote: ... The virus was propagated to passage three on Vero E6 cells in Opti-MEM I (1X) + GlutaMAX (Gibco), supplemented with penicillin (10,000 IU/mL ...
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bioRxiv - Microbiology 2019Quote: FVG-R infections were performed by inoculating A549 cells with virus diluted in Opti-MEM I (Invitrogen, 31985070) supplemented with 0.2% FBS ...
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bioRxiv - Microbiology 2023Quote: ... The cell and virus lysates were Western blotted and probed with a goat polyclonal anti-gp120 antibody (Invitrogen), the 4E10 anti-gp41 antibody (NIH HIV Reagent Program ...
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bioRxiv - Cancer Biology 2023Quote: ... Epstein–Barr virus-transformed lymphoblasts were obtained from Coriell Cell Repositories and were grown in RPMI 1640 (Gibco) supplemented with 10% FBS and penicillin/streptomycin (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... Stock virus was sub-passaged through Madin-Darby canine kidney (MDCK) cells in Dulbecco’s modified Eagle’s medium (DMEM; Gibco), harvested as tissue culture supernatant and viral titres determined by cytopathic effects on MDCK cells and expressed as the mean log10 tissue culture infective dose required to kill 50% of the cells (TCID50 ...
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bioRxiv - Neuroscience 2024Quote: Human fibroblast cells were reprogrammed using the Sendai virus-based CytoTune iPS 2.0 kit (CytoTune 2.0, Invitrogen, A16517). Fibroblasts were plated in six-well plates two days before transduction to achieve a density of 2 × 105 to 3 × 105 cells per well ...
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bioRxiv - Biochemistry 2023Quote: ... 8 ml P4 virus was used to infect 800 ml cultures of Expi293F GnTI-Cells (Thermo Fisher Scientific) cells at 2-3 × 106 cells supplemented with 1% Fetal Bovine Serum (FBS) ...
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bioRxiv - Genomics 2024Quote: ... Virus was produced by transfecting HEK293T cells with the plasmid and the Lipofectamine 3000 kit (Thermo Fisher Scientific). U87MG were transduced with the supernatant containing the NY-ESO-1 virus ...