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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: The Microtubule Associated Protein 2 (MAP2) (Thermo Fisher Scientific cat ...
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bioRxiv - Biochemistry 2024Quote: ... were infected with the virus at a cell density of 4.0 × 106 cells per milliliter in Sf900 II medium (Gibco), and grown for 48 h at 27 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... then transferred into Grace’s Insect Medium containing 0.25mg/ml BrdU (Invitrogen B23151) and incubated at 25°C for 90 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... larval brains were dissected in insect media (HyClone SFX; Thermo Fisher Scientific), incubated in 0.5% sodium citrate ...
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bioRxiv - Genomics 2023Quote: ... All cultures were grown in supplemented Grace’s Insect Media (ThermoFisher Scientific #11605094) with 5% fetal bovine serum added (ThermoFisher Scientific #10437010 ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... braziliensis (MHOM/BR/01/BA788) were grown in Schneider’s insect medium (GIBCO) supplemented with 20% heat-inactivated FBS (Sigma ...
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bioRxiv - Immunology 2021Quote: ... T2 target cells and Epstein-Barr Virus-transformed B lymphoblastoid cell lines (EBV-LCLs) cells were cultured in RPMI-1640 (Thermo Fisher Scientific) supplemented with 10% FCS and 1% penicillin/streptomycin (#P433 ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
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bioRxiv - Immunology 2024Quote: ... Infection was initiated by adding 100 µl of virus inoculum containing 100.000 PFU (MOI ∼0.5-1) in virus absorption medium (Gibco OptiPro Serum free medium + 1:100 Glutamax + 10 mM HEPES ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: HeLa cell pellets (∼2×107) or MCF7 cell pellets (∼4×107) were lysed with 3 mL Mammalian Protein Extraction Reagent (Thermo Scientific), supplemented with 2X HALT protease inhibitor (Thermo Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Copies of SARS-CoV-2 E gene were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA was detected with the CDC assay kit (IDT) and the TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on the QuantStudio3 System (Invitrogen).
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bioRxiv - Pathology 2021Quote: ... 10 µl of rSARS-CoV-2 NL virus stock was diluted in 10 ml of OptiMEM media (ThermoFisher Scientific, #51985091) and then added to cells ...
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bioRxiv - Microbiology 2020Quote: ... Vero FM cells seeded in 24-well plates were infected in duplicate with 100 μl 2-fold serial dilutions of patient material in a total volume of 200 μl using virus isolation medium [DMEM (Gibco) containing ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 until the following day. Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat. no. 61965026 supplemented with 2% FBS (Gibco, Cat ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 until the following day. Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat. no. 61965026 supplemented with 2% FBS (Gibco, Cat ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with each plasmid expressing SPΔC of SARS-CoV-2 Delta variant (1.2 µg DNA) or human influenza A virus M2 (5 µg DNA) using Lipofectamine 3000 (Invitrogen, USA) to produce 293TN-S target cells ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 S protein-expressing plasmids were transfected into HEK293T cells using Lipofectamine 3000 (Invitrogen, USA). 24 h after transfection ...
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bioRxiv - Immunology 2021Quote: ... or B.1.351 Spike protein (Table 2) were produced using HEK293T cells transfected with Lipofectamine 3000 (ThermoFisher) using IgE-SARS-CoV-2 S plasmid variants (Genscript ...
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bioRxiv - Cancer Biology 2021Quote: ... envelope plasmid pIP/VSV-G (5μg) (ViraPower™ Lentiviral Packaging Mix, Thermo Fisher Scientific) and 5μg lentiviral expression constructs shRNA (pLKO.1 Mission shRNA DNA clone ...
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bioRxiv - Cell Biology 2020Quote: ... 2.5µg psPAX2 packaging plasmid and 1µg pMD2.G envelope plasmid using Lipofectamine 2000 (Invitrogen). Lentiviral supernatant was collected ...
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bioRxiv - Molecular Biology 2020Quote: ... pMD2-VSVG (envelope vector) and pCDH plasmids co-transfection using Lipofectamine 2000 (#11668019, Invitrogen). Medium containing virus was harvested 48 h after transfection and filtered by 0.45 μm millipore filter ...
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bioRxiv - Genomics 2022Quote: ... psPAX2 packaging plasmids and pMD2.G envelope plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... envelope plasmid pIP/VSV-G (5 µg) (ViraPowerTM Lentiviral Packaging Mix, Thermo Fisher Scientific), 5 µg of lentiviral expression construct shRNA (pLKO.1 Mission shRNA DNA clone ...
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bioRxiv - Cancer Biology 2023Quote: ... and the envelope vector pVSV-G using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 600 ng VSV-G envelope plasmid and 6 μL of Lipofectamine 2000 (Thermo Scientific). Supernatants were collected at 48 and 72 h post-transfection and clarified using 0.45 μm filters ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... Pelleted virus was resolved in PBS (Gibco) and stored at −80°C until further usage ...
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bioRxiv - Immunology 2019Quote: ... murine leukemia virus reverse transcriptase (RT; Invitrogen), and random hexamers (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... Fibroblasts were reprogrammed using Sendai virus (Invitrogen) in feeder-free conditions on Matrigel (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... and EmGFP reporter Sendai Virus (A16519, Invitrogen) were part of the Cytotune -iPS 2.0 Sendai Reprogramming kit and EmGFP Sendai Fluorescence Reporter kit ...
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bioRxiv - Immunology 2024Quote: ... in virus growth medium (1X MEM [Gibco 11095080] ...
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bioRxiv - Cell Biology 2020Quote: ... pseudokinase domain of mouse and human MLKL were expressed in Sf21 insect cells using the Bac-to-Bac system (ThermoFisher Scientific) and purified using established procedures28 ...
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bioRxiv - Biophysics 2021Quote: ... MVIRRL/AAA and Xenopus calmodulin were expressed in Sf9 and Sf21 (Spodoptera frugiperda) insect cells using the Bac-to-Bac® Baculovirus Expression System (Invitrogen). Sf9 cells were cultured in Sf900 media (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... BmN cells was preserved in our laboratory and cultured in TC100 insect cell culture medium (Applichem, Darmstadt, Germany) with 10% fetal bovine serum (FBS, Gibco, USA) at 27°C ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant baculoviruses expressing Abl2 or Abl2 fragments in pFastBac were generated using the Bac-to-Bac expression system in Sf9 insect cells according to the manufacturer’s instructions (ThermoFisher, Waltham, MA). After 36-48 hr infection with baculoviruses ...
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bioRxiv - Immunology 2022Quote: The α−TD monoclonal antibody was produced in Sf9 insect cells by using a Bac-to-Bac recombinant baculovirus system (Invitrogen). The monoclonal antibody manufacturing process started with the revival ...
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bioRxiv - Biochemistry 2020Quote: ... The expression of HER2 domain IV in insect cells was carried out using a Bac-to-Bac® Baculovirus Expression System (Invitrogen). The resulting construct was expressed in Spodoptera frugiperda (Sf9 ...
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bioRxiv - Cell Biology 2020Quote: ... and Xenopus calmodulin were expressed in Sf9 and Sf21 (Spodoptera frugiperda) insect cells using the Bac-to-Bac® Baculovirus Expression System (Invitrogen). Sf9 cells were cultured in Sf900 media (Gibco) ...
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bioRxiv - Genomics 2021Quote: Full-length Sen1 was overexpressed from pFL vector in insect cells (Trichoplusia ni) using an optimized synthetic gene (GeneArt, Life Technologies) and the baculovirus expression system (Berger et al. ...
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bioRxiv - Cell Biology 2023Quote: ... was cloned as fusion protein with an N-terminal His6-rsEGFP2-tag and a C-terminal His6-tag in pFastBacDual between the KpnI and HindIII sites and expressed in Sf21 insect cells using the Bac-to-Bac expression system (Thermo Fisher). The protein was purified by affinity chromatography on HisTrap FF in 1x PBS + 880 mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and USP33c36–825 were overexpressed in Sf9 cells where cultures were grown in HyQ SFX Insect Serum Free Medium (Fisher Scientific) to a density of 4 x 106 cells/mL and infected with 10 mL of P3 viral stock per 1 L of cell culture ...
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bioRxiv - Cancer Biology 2023Quote: ... All USP18 variants were expressed in Spodoptera frugiperda (Sf9, ATCC CRL-1711) insect cell using the Bac-to-Bac Baculovirus Expression System (Invitrogen, 10359016). Sf9 cells were infected at a cell density of 4 × 106cells/mL with a multiplicity of infection of 5.0 ...
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bioRxiv - Biochemistry 2023Quote: ... human GRK2 S670A with a C-terminal 6xHIS tag was expressed using the Bac-to-Bac insect cell expression system (Life Technologies). The insect cells were harvested 48 hours post-infection and homogenized with buffer containing 20 mM HEPES (pH 8.0) ...
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bioRxiv - Microbiology 2023Quote: ... large scale suspension cultures expressing the polymerase fusion constructs of A/Victoria/3/1975(H3N2) was prepared using High Five insect cells grown in Express Five media (Life Technologies) at 0.5 × 106 cells/mL infected at 0.2% (V/V ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... For both fungi, first we obtained fresh blastospores (i.e., yeast-like single cells) suspended in Grace’s Insect Medium (Gibco, Thermo Fisher Scientific) supplemented with 2.5% FBS (Gibco ...
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bioRxiv - Biophysics 2023Quote: High-titer recombinant baculovirus (>109 viral particles per mL) for A2AAR expression in Sf9 insect cells was obtained following a modified Bac-to-Bac system protocol (Invitrogen, USA). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of the cell lysate protein and equal volume of the virus pellet protein were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris gel; Invitrogen/Thermo Scientific) and transferred onto nitrocellulose membrane by semidry transfer ...
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bioRxiv - Microbiology 2019Quote: ... The viral N-protein was labeled using a primary anti-Tula virus antibody(12) and a secondary anti-rabbit FITC conjugate (Invitrogen, USA). The viral Gc protein was stained using a mouse monoclonal antibody (#H1808-60B ...
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bioRxiv - Biochemistry 2021Quote: ... Individual reactions contained 2 μM protein and 5X SYPRO Orange Protein Gel Stain (Invitrogen). DP7/maltoheptaose (Elicityl) ...
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bioRxiv - Biochemistry 2019Quote: ... lentivector plasmids were transfected into HEK293T cells together with packaging and envelope plasmids (psPAX2 and pMD2.G) using Lipofectamine 3000 (Thermo Fisher Scientific, L3000008). Three days after transfection ...