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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The RNA concentrations of 30 samples were normalized to approximately 15 ng µl-1 and TSV was detected and quantified from 2 µl of sample using a TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems™) following the OIE recommended protocol and triplicate standards (mean R2 = 0.93 ± 0.06 ...
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Evolutionary stasis of an RNA virus indicates arbovirus re-emergence triggered by accidental releasebioRxiv - Evolutionary Biology 2019Quote: ... and virus isolates using Trizol LS® (Invitrogen, USA) and purified using Direct-zol RNA MiniPrep (Zymo Research ...
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bioRxiv - Cell Biology 2022Quote: ... or Sendai virus system Cytotune 2.0 (Thermo Scientific #A16517). Resulting iPSCs were clonally picked and expanded in mTESR (Stemcell Technologies ...
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bioRxiv - Microbiology 2019Quote: ... The virus inoculum was prepared in OPTI-MEM (Gibco), and cells were incubated in SMEM at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Pelleted virus was resuspended in PBS and TRIzol (Invitrogen) extracted ...
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bioRxiv - Microbiology 2022Quote: ... and from virus in supernatant using Trizol LS (ThermoFisher). A one-step qRT-PCR kit with SYBR green from Agilent was used ...
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bioRxiv - Microbiology 2021Quote: ... virus stocks were diluted in OptiPRO SFM (ThermoFisher Scientific) serum-free medium according to the desired MOI ...
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bioRxiv - Microbiology 2020Quote: ... The virus pellet was washed in HBSS (Thermo Fisher), and centrifuged at 100,000 × g (Beckman SW 32 Ti rotor ...
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bioRxiv - Immunology 2022Quote: ... and Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen), followed by RT-PCR with Fast SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Immobilised virus was fixed with 4% formaldehyde (Thermo Scientific) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... TaqMan Fast Virus 1-step Master Mix (Applied Biosystems) and primer/probes targeting NS3 primers/probe (IDT)11 ...
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bioRxiv - Microbiology 2024Quote: ... and TaqMan Fast Virus 1-Step Master Mix (ThermoFisher). Reactions were set up according to the manufacturer’s protocols using a 10 µl reaction volume and 25 ng of total RNA template ...
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bioRxiv - Cancer Biology 2024Quote: ... virus was prepared in high-glucose DMEM (Gibco, #11965092) supplemented with 10% FBS and 1% penicillin–streptomycin ...
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bioRxiv - Biochemistry 2019Quote: ... the bacmids were transfected into the Sf9 insect cells using a Bac-to-Bac baculovirus expression system according to the manual (Thermo Fisher Scientific, Langenselbold, Germany). The titer of the recombinant virus was determined following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Spodoptera frugiperda ovarian epithelial cells (Sf9, ATCC CRL-1711) were cultured at 28 °C in Grace insect medium (Gibco, Thermo Fisher Scientific, Waltham, USA) supplemented with 10% fetal calf serum (FCS) ...
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bioRxiv - Microbiology 2021Quote: ... MHV-A59 was quantified by RT-qPCR by amplifying a fraction of its ORF5 gene for the structural protein M.15,16 The TaqMan™ Fast Virus 1-Step Master Mix Kit (Thermo Fisher Scientific Inc., 4444432) was used for RT-qPCR ...
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bioRxiv - Immunology 2021Quote: The RBDwt protein was conjugated to Cucumber mosaic virus - tetanus toxoid (CuMVTT) using the linker Succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, Waltham, USA). CuMVTT contains a powerful T cell epitope of the Tetanus toxin (TT ...
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bioRxiv - Microbiology 2021Quote: ... and S protein expressing pcDNA3.1-SARS CoV-2 S.dCT were co-transfected into HEK293T cells using lipofectamine 2000 (Thermo Fisher Scientific). After 48 hours ...
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bioRxiv - Bioengineering 2020Quote: ACE-Fc fusion proteins and the SARS-CoV-2 RBD were expressed in suspension-HEK293F cells grown in FreeStyle media (Gibco). Transfections were done using 40 kDa polyethyleneimine (PEI-MAX ...
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bioRxiv - Microbiology 2021Quote: ... and S protein expressing pcDNA3.1-SARS CoV-2 SΔCT were co-transfected into HEK293T cells by lipofectamine 2000 (Thermo Fisher Scientific). The supernatants containing the pseudotype viruses were collected 48 h after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... HEp-2 cells were transiently transfected with an expression vector of fluorescently labeled proteins using Lipofectamine LTX & Plus Reagent (Invitrogen) for 48 h before stress exposure ...
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bioRxiv - Microbiology 2021Quote: ... cells transfected with the SARS-CoV-2 S protein deletion mutants and controls were washed once with DPBS (Fisher Scientific), fixed with 4% (w/v ...
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bioRxiv - Immunology 2020Quote: The prefusion ectodomain of the SARS-CoV-2 Spike protein was transiently transfected into suspension-adapted ExpiCHO cells (Thermo Fisher) with PEI MAX (Polysciences ...
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bioRxiv - Immunology 2022Quote: IFNγ mRNA and protein were measured in IL-2 expanded MAIT cells (with or without stimulation with CD3/CD28 Dynabeads (Gibco)) and their culture supernatant by qPCR or ELISA respectively ...
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bioRxiv - Immunology 2023Quote: ... intracellular staining was performed for 2 h at 4°C after stimulation for 2 h with PMA/Ionomycin Cell Stimulation Cocktail containing protein transport inhibitors (ThermoFisher). Data were acquired on an Aurora (Cytek Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... Purifications were carried out from 150 μl of the whole-cell extract (2 mg protein) per experiment using 37 µl of Dynabeads M-280 sheep anti-rabbit IgG (Invitrogen) and 2 µl of the anti-LytA antibody (Biomedal) ...
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bioRxiv - Cancer Biology 2022Quote: ... 266-6 CAS9 cells were infected with FUW-TetO-KRASG12D-BleoR virus and cells were selected with 5 mg/ml zeocin (Invitrogen, San Diego, CA) for 10 days ...
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bioRxiv - Neuroscience 2020Quote: Cell surface proteins were purified using the Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific) based on manufacturer instructions with a few modifications ...
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bioRxiv - Microbiology 2021Quote: ... 2×106 cells/ml EXPI293F cells (Life Technologies) were transfected with 1 μg DNA/ml cells in a 3:2 (LC:HC ...
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bioRxiv - Cell Biology 2020Quote: ... to label cell surface proteins (Invitrogen) (53) ...
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bioRxiv - Cell Biology 2019Quote: Whole ovaries were dissected in room temperature Grace’s insect media (Thermo Fisher Scientific, Waltham, MA). Active-MRLC staining was performed using a protocol provided by Jocelyn McDonald (Majumder et al. ...
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bioRxiv - Developmental Biology 2019Quote: For live imaging 3-5 day old flies were dissected in Schneider’s Insect Media (Thermofisher) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... Wing imaginal discs were dissected in either PBS or Grace’s Insect Media (Thermo Fisher Scientific) from wandering third instar larva ...
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bioRxiv - Microbiology 2020Quote: ... The hemocyte pellet was washed twice using 1 mL cold Grace’s insect medium (Gibco, USA) supplemented with 10% FBS (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were loaded with 2 μM Fura-2/AM (Invitrogen). Ratiometric Ca2+ imaging was performed at 340 and 380 nm in 0 or 2 mM Ca2+ Ringer’s solution using an IX81 microscope (Olympus ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated with 2 μM Fura-2 (F1221, Invitrogen) at 37℃ for 30 min dispersed in Margo solution with 0.2‰ w/v Pluronic F-127 ...
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bioRxiv - Microbiology 2021Quote: ... that had been previously labeled with Alexa-488 (anti-serotype 4-Alexa-488, to stain TIGR4) or Alexa-555 (anti-serogroup 19-Alexa-555, to stain GA47281) (Molecular Probes). Stained preparations were finally washed two times with PBS ...
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bioRxiv - Immunology 2023Quote: ... and H1N1/2009 influenza virus strains were prepared by overnight incubation of the virus strains with 0.1% v/v β propiolactone (Acros Organics, Geel, Belgium) under continuous rotation at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were eluted in 2× NuPAGE LDS Sample Buffer (Life Technologies) by boiling at 95 °C for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Mouse IFN-alpha 2 (Ifna2) recombinant protein was purchased from Affymetrix eBioscience (catalog #14-8312) ...
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bioRxiv - Molecular Biology 2021Quote: ... pre-cleared for 2 h with Protein A Dynabeads (Invitrogen, 10001D), followed by overnight incubation with 5 µg of antibody (anti-SKIV2L Proteintech group ...
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bioRxiv - Microbiology 2021Quote: ... or SARS-CoV-2 E protein (1-75 aa) (Thermo Fisher) in coating buffer [0.05 M sodium carbonate–sodium bicarbonate (pH 9.6)] ...
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bioRxiv - Cell Biology 2020Quote: ... Fura-2 BZiPAR and Protein G magnetic beads were from Invitrogen - Thermo Fisher Scientific (Waltham ...
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bioRxiv - Physiology 2021Quote: ... mouse-anti Microtubule-associated protein 2 (MAP2) (Invitrogen, Cat # 13-1500) (1:1000) ...
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bioRxiv - Neuroscience 2020Quote: ... lysosome-associated membrane protein 2 (LAMP2; 1:1000; Thermofisher, Waltham, MA), or phopsho-ribosomal S6 protein (PS6 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were revealed using ECL reagent (ECL 2, Thermo Scientific) with a Typhoon 9419 phosphorimager (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... supernatant proteins (2 mg/ml) were reduced with 25mM TCEP (ThermoFisher) for 1 h at 55°C ...
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bioRxiv - Microbiology 2019Quote: ... Initial virus was recovered from T7 transcribed RNA using reverse transfection of Huh7.5.1 cells by Lipofectamine® 2000 (Invitrogen) (for virus rescue ...
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were generated by transfecting the clone into Vero cells using Lipofectamine 2000 (Thermo Fisher, cat# 11668-019), and harvested at various times post-transfection.
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bioRxiv - Biophysics 2021Quote: ... the protein was expressed as a fusion construct with an N-terminal His6 tag followed by tobacco etch virus (TEV) protease cleavage site in E.coli BL21 DE3 Star cells (Invitrogen). The fusion construct was purified using immobilized-nickel affinity chromatography ...