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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: His-CLASP1 protein was expressed in baculovirus-infected Sf9 insect cells using the Bac-to-Bac system (Invitrogen). After the first amplification ...
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bioRxiv - Cell Biology 2023Quote: ... commercial insect cell lines used this study include Sf9 cells (Invitrogen) and Tni (High-Five ...
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bioRxiv - Molecular Biology 2020Quote: ... into Sf9 insect cells (Thermo Fisher Scientific, MA, USA), according to the protocol provided by the manufacturer (Expression Systems) ...
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bioRxiv - Biophysics 2021Quote: Sf9 insect cells (Thermo Fisher Scientific, Waltham, MA, USA) were cultured in SIM SF (Sino Biological ...
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bioRxiv - Biophysics 2021Quote: ... were co-expressed in High Five insect cells (Invitrogen) using the Bac-to-Bac baculovirus expression system ...
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bioRxiv - Microbiology 2022Quote: S2 cells were cultured in Schneider’s insect medium (Gibco) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Biophysics 2019Quote: ... Spodoptera frugiperda (Sf21) insect cells were purchased from Invitrogen. Bacterial expression plasmid pProEx HTc was purchased from Invitrogen ...
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bioRxiv - Genomics 2022Quote: Sf9 insect cells were purchased from Invitrogen (Waltham, MA), and initial p0 culture was thawed and recovered as per manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Drosophila S2 cells grown in Schneider’s insect medium (Gibco) with 10% FBS were first 1:1 passaged using serum free sf-900 II SFM medium (Gibco ...
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bioRxiv - Biophysics 2023Quote: ... and transfected into Sf9 insect cells (Gibco; Cat. 11496015) with Cellfectin II Reagent (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... HCoV-19 S protein was expressed in Sf9 insect cells using the Bac-to-Bac system (Life Technologies Inc.) and harvested from cell culture medium followed by purification procedure using Ni-NTA column and Superdex 200 gel filtration column (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: The MBP-TOPOVIBLΔC25 protein was produced in Sf9 insect cells using the bac-to-bac baculovirus expression system (Invitrogen). Mouse Top6bl cDNA was first amplified from the pl159 plasmid (Table S4 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and High Five (Trichoplusia ni; Boyce Thompson Institute at Cornell University) (40) insect cells were grown in Grace’s insect medium (Invitrogen) with 10% FCS and Express Five insect serum-free medium (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Proteins from virus-infected cells were immunoprecipitated using protein G magnetic Dynabeads according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Briefly ...
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bioRxiv - Immunology 2019Quote: ... High Five insect cells were maintained at a cell density of 0.5–1 × 106 cells/mL in Express Five medium (Life Technologies) supplemented with 16mM L-glutamine ...
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bioRxiv - Immunology 2023Quote: Spodoptera frugiperda Sf9 insect cells (ATCC CRL-1711) were maintained as suspension cultures in Sf900-II insect serum free medium (ThermoFisher) at 28 ◦C ...
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bioRxiv - Developmental Biology 2022Quote: PLP F2-3 (aa 584 to 1811) protein was isolated from SF9 insect cells using Bac-to-Bac baculovirus expression system (Thermofisher). Coding sequence of PLP F2 -3 was cloned into pFastBac donor plasmid containing a Flag tag at the C-terminus of the insert site of PLP (Gift from Rong Liu ...
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bioRxiv - Cell Biology 2021Quote: Human ProtoArray® Microarray (v5.0) containing 9483 unique human proteins expressed as GST-fusion in insect cells were purchased from Invitrogen. Arrays were blocked in 4°C cold blocking buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: The human cDNA encoding zDHHC2 was codon optimised for protein expression in insect cells and synthesized by GeneArt technology (Invitrogen). It was inserted with N and C-terminal tags (His6 and StrepII ...
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bioRxiv - Microbiology 2020Quote: A baculovirus expression system was used for the expression of DUB2 recombinant protein in sf9 insect cells as described in the Bac-to-Bac Baculovirus Expression protocol (Invitrogen). The gene was first cloned into the PFastBacNKI-his-3C-LIC vector using Ligation Independent Cloning (LIC) ...
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bioRxiv - Biochemistry 2023Quote: ... and p300BRPHZT 4A- and 4E-substituted mutant proteins were expressed in baculovirus-infected High Five insect cells (Thermo Fisher Scientific). Baculoviruses were produced using the Bac-to-Bac baculovirus expression system (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... we used the Bac-to-Bac Baculovirus Expression System (Thermo 10359016) to grow baculoviruses and express proteins in High Five insect cells (Thermofisher). Sf21 cells were used for baculovirus propagation and Hi5 cells were used for protein expression ...
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bioRxiv - Biochemistry 2024Quote: ... Protein expression was done in Spodoptera frugiperda (Sf9) insect cells (ATCC) using the Bac-to-Bac Baculovirus Expression System (Invitrogen). The insect cells were infected at a density of 2-3 × 106 cells/ml with high titer viral stock multiplicity of infection (MOI ...
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bioRxiv - Biochemistry 2021Quote: Sf9 and High Five insect cells that purchased from Invitrogen were used for baculovirus propagation and protein expression ...
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bioRxiv - Biochemistry 2020Quote: ... High Five (Hi5) insect cells (BTI-TN-5B1-4 Invitrogen) were co-infected with two or three baculoviruses 1/100 (v/v ...
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bioRxiv - Microbiology 2020Quote: ... Sf9 cells were adherently cultured in Grace’s Insect Medium (Gibco) supplemented with 10% FBS in plates at 28°C ...
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bioRxiv - Microbiology 2022Quote: ... and then maintained in Grace’s Insect Cell Culture Medium (Gibco) containing 50% fetal bovine serum (Biological Industries) ...
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bioRxiv - Biochemistry 2023Quote: ... Purified bacmid DNA was transfected into Sf9 insect cells (Invitrogen) to produce secreted recombinant baculovirus ...
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bioRxiv - Biophysics 2023Quote: ... and scFv16 co-infected Hight Five (Hi5) insect cells (Invitrogen) in logarithmic phase at a 1:1:1:1:1 ratio ...
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bioRxiv - Biochemistry 2024Quote: All constructs used for infection of SF9 Insect Cells (Gibco) were codon optimized and cloned into vector pVL1393 (MTI Bio) ...
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bioRxiv - Microbiology 2023Quote: ... PA (1:250), PB2 (1:250), NS1 (1:250, PA5-32243 and NS2 (1:250, PA5-32234) were all purchased from Thermo Scientific, Rockford ...
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bioRxiv - Molecular Biology 2022Quote: ... The baculovirus was produced by transfecting 1 ml of Sf9 insect cells (0.9 × 106 cells/ml in Grace medium, Invitrogen) with 45 µg of recombinant bacmid carrying the DNA-encoding sequences for Wuhan-Hu-1 ...
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bioRxiv - Neuroscience 2021Quote: ... cells and transfected into Sf9 insect cells using Cellfectin II reagent (ThermoFisher, 10362100). Baculovirus was generated according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... We employed the MultiBac (Geneva Biotech, Indianapolis, IN, USA) system for protein expression in insect cells (Sf9, Invitrogen, Burlington, ON, Canada) according to published protocols (38,39) ...
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bioRxiv - Microbiology 2023Quote: ... MCC virus-positive MKL-1 cells were cultured in 1x RPMI (ThermoFisher Scientific) supplemented similarly as stated above ...
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bioRxiv - Cell Biology 2019Quote: Stably expressing S2 insect cells were selected using puromycin (Life Technologies). Cells were cultured under sterile conditions at 28°C in Schneider’s Drosophila medium (Lonza) ...
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bioRxiv - Immunology 2022Quote: ... Each vector was individually transfected into SF9 insect cells (Thermo Fisher) with BestBac 2.0 linearized baculovirus DNA (Expression Systems ...
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bioRxiv - Developmental Biology 2022Quote: ... The infected Sf9 cells were incubated in Grace’s insect medium (Invitrogen) supplemented with 10% FBS at 27℃ for 3 d ...
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bioRxiv - Biochemistry 2021Quote: ... ACKR1 constructs were also expressed in Sf9 insect cells (Life Technologies) using the pFastBac baculovirus system (Thermofischer) ...
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bioRxiv - Molecular Biology 2022Quote: ScFv16 was expressed in High-Five™ insect cells (ThermoFisher Scientific) as a secreted protein purified by Ni-sepharose chromatography column as described previously44 ...
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bioRxiv - Biochemistry 2024Quote: The jsGiq heterotrimer was expressed in High Five insect cells (Invitrogen). Large-scale expression was conducted in High Five insect cells at a density of 4.0 x 106 cells/ml in SF900II SFM medium using 5 L Erlenmeyer flasks (Corning) ...
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bioRxiv - Molecular Biology 2023Quote: Full length (aa 1-691) and truncated (aa 1-321) RTA sequences were expressed and purified using SF9 insect cell expression system (ThermoFisher). Gene sequences were based on JSC-1 sequence (accession GQ994935) ...
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bioRxiv - Biophysics 2021Quote: ... Sf9 insect (Thermo Fisher Scientific) cells were cultured in Sf-900 III SFM medium (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Sf9 insect (Thermo Fisher Scientific) suspension cells were cultured in Sf-900 III SFM medium (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Concentrated virus was then incubated using 1:2000 DiD-Cell labelling solution (ThermoFisher V22887) for 20 mins at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... Baculoviruses were generated and amplified in Sf9 insect cells (Thermo Fisher Scientific) using the EMBacY baculoviral genome (79) ...
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bioRxiv - Biochemistry 2021Quote: ... Baculoviruses were generated and amplified in Sf9 insect cells (Thermo Fisher Scientific) using the EMBacY baculoviral genome [64] ...
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bioRxiv - Biophysics 2019Quote: Recombinant APC/C was expressed in High Five insect cells (Thermo Scientific) as described in30,42 ...
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bioRxiv - Biochemistry 2019Quote: Sf9 insect cells were grown in Sf-900 II medium (Thermo Fisher) at 27 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Trichoplusia ni High Five (Hi5) insect cells (BTI-TN-5B1-4 Invitrogen) were co-infected with two baculoviruses 1/100 each ...