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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
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bioRxiv - Microbiology 2024Quote: ... NS1 proteins were detected using DENV2 NS1 polyclonal antibody (1:1,000 ThermoFisher Scientific) with overnight incubation at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Antibodies used: anti-Influenza A virus NS1 (PA5-32243, ThermoFisher), anti-acetyl-α-tubulin (Ly640 ...
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bioRxiv - Biochemistry 2019Quote: ... Recombinant virus was made by co-transfection into SF9 insect cells (Invitrogen) of the plasmid and BacVector3000 baculovirus DNA (Novagen ...
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bioRxiv - Immunology 2021Quote: ... NS1 (Invitrogen Cat #PIPA532243, 1:1000), NP (BioRad Cat#MCA400 ...
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bioRxiv - Plant Biology 2019Quote: ... The protein was expressed in sf21 insect cells using the vector pFastBac 1 (Invitrogen). One litre of cells (2.5×106 cells ml−1 ...
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bioRxiv - Cell Biology 2023Quote: High titer virus expressing Flag-WRN was used to infect Hi5 insect cells (Thermo Fisher Scientific) at an MOI of approximately 10 ...
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bioRxiv - Molecular Biology 2019Quote: ... the proteins were overexpressed in Hi5 insect cells (Thermofisher Scientific), harvested by centrifugation at 3000 rpm ...
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bioRxiv - Biophysics 2019Quote: ... protein-free insect cell medium with L-glutamine (Thermo Fisher Scientific) at 27°C ...
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bioRxiv - Immunology 2021Quote: ... Sf9 insect cells (Gibco) were transduced with each transfer plasmid ...
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bioRxiv - Microbiology 2020Quote: ... Sf9 insect cells (Invitrogen) were then transfected with the bacmids to produce the initial stock of baculovirus ...
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bioRxiv - Biochemistry 2023Quote: ... PTH1R and G proteins were co-expressed in Sf9 insect cells (Invitrogen). The Sf9 cells grew to a density of 3.5 × 106 cells/mL in ESF 921 cell culture medium (Expression Systems ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant P0 virus for each gene were generated in ExpiSf9 insect cells following the manufacturer’s recommendations (Thermo Fisher). The amount of each virus needed for infection has been optimized for maximal production of the two proteins ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight with either Dengue 2 fixed virus antigen (Microbix, Cat# EL-22-02) or polyvalent total immunoglobulin (Invitrogen, Cat# H17000). Before adding PBMC’s ...
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bioRxiv - Microbiology 2023Quote: ... anti-NS1 and anti-NS2 (all 1:2000, Thermo Fisher). Membranes were washed in PBS-T and incubated with fluorescent secondary antibody (anti-mouse or anti-rabbit conjugated to AlexaFluor488 or 647 ...
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bioRxiv - Microbiology 2022Quote: ... was used as starting material for protein expression in insect cells (Sf9, Invitrogen). We employed the MultiBac (Geneva Biotech ...
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bioRxiv - Biochemistry 2022Quote: The PTH1R and G proteins were co-expressed in Sf9 insect cells (Invitrogen). When the cells grew to a density of 3.5 × 106 cells/mL in ESF 921 cell culture medium (Expression Systems) ...
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bioRxiv - Microbiology 2020Quote: ... The dengue 2 E protein antibody PA5-32246 was used at 1:10,000 for western blotting (Thermo-Fisher Scientific, Waltham, MA). Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL ...
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bioRxiv - Microbiology 2022Quote: ... 4a3A cells were maintained at 27°C without CO2 in Insect-XPRESS™ Protein-free Insect Cell Medium complemented with heat-inactivated Fetal Calf Serum (FCS) (#A3840002 ThermoFisher Scientific). C6/36 cell line (ATCC ...
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bioRxiv - Biochemistry 2021Quote: ... Sf9 insect cells (Gibco™) were transduced with the recombinant purified plasmids ...
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bioRxiv - Microbiology 2021Quote: High Five insect cells (Invitrogen) maintained in Express Five serum free medium (Life Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... D.mel-2 insect cells (ThermoFisher) were co-transfected with the expression construct and blasticidin resistance marker pCoBlast (ThermoFisher ...
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bioRxiv - Plant Biology 2022Quote: ... of Sf21 insect cells (Invitrogen). Baculovirus was generally amplified to the P2 generation before protein expression ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA from either wild type dengue plasmid or mutated dengue plasmid were quantified using a High Sensitivity Range Qubit (Thermo Fisher). Library preparation was carried out with the Nextera XT DNA Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... Sf9 insect cells were grown at 27°C in Grace’s Insect Cell Culture Medium (Gibco) supplemented with 4.175mM sodium bicarbonate ...
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bioRxiv - Biochemistry 2019Quote: ... MePCE and HEXIM1 protein was produced by infection of High Five insect cells (Invitrogen) at high multiplicity of infection ...
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bioRxiv - Molecular Biology 2023Quote: MCHRs and G proteins were co-expressed in High Five insect cells (Thermo Fisher) using Bac-to-Bac baculovirus system (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... S2 cells were incubated in insect medium (HyClone SFX-Insect Cell Culture Media; Fisher Scientific, SH3027802) supplemented with penicillin (50 units/mL ...
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bioRxiv - Biochemistry 2020Quote: ... Sf9 insect cells (Thermo Fisher Scientific) were cultured in SIM SF (Sino Biological ...
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bioRxiv - Biophysics 2020Quote: Sf9 insect cells (Thermo Fisher Scientific) were cultured in SIM SF (Sino Biological ...
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bioRxiv - Biochemistry 2024Quote: Sf9 insect cells (Thermo Fisher Scientific) were cultured in serum-free Ex-cell 420 medium (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... Spodoptera frugiperda Sf9 insect cells (Invitrogen) were grown in suspension at 27°C ...
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bioRxiv - Immunology 2019Quote: ... The protein was expressed in Sf9 insect cells using a recombinant baculovirus system (Life Technologies). The protein was purified on a cobalt resin column and further purified by gel filtration to ensure the removal of monomeric and aggregated protein.
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bioRxiv - Immunology 2021Quote: ... Dengue viruses were grown in C6/36 cells cultured in RPMI 1640 (Gibco 11875-093), L-Glutamine (Gibco 25030-081) ...
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bioRxiv - Cell Biology 2022Quote: Human proteins (LIS1-SNAP, or motor domains) were expressed and purified from insect cells (ExpiSf9 cells; Life Technologies) as previously described with minor modifications3,7,20,69 ...
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bioRxiv - Microbiology 2021Quote: ... were transfected with pcineo plasmids (0.4 μg) carrying the RSV codon-optimised NS1 or FLAG-NS1 WT or mutant constructs using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... High Five™ insect cells (Thermo Fisher) were used to produce DCL3 using baculovirus mediated protein expression (MOI = 1.5) ...
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bioRxiv - Immunology 2021Quote: ... ExpiSf9 insect cells were purchased from ThermoFisher Scientific;
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bioRxiv - Molecular Biology 2022Quote: ... in Spodoptera frugiperda (Sf9) insect cells (Invitrogen). P0 viral stock was produced by transfecting 5 μg recombinant bacmids into Sf9 cells (2.5 mL ...
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bioRxiv - Biophysics 2023Quote: High Five™ insect cells (Thermo Scientific) were grown at 27 °C and 100 rpm agitation in ESF921™ insect cell-culture medium (Expression Systems) ...
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bioRxiv - Cell Biology 2024Quote: Kif9 fused to GFP (eGFP-Kif9) protein was expressed and purified from SF9 insect cells (Thermofisher). MTs purified from bovine brain tubulin were used for all motility experiments ...
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bioRxiv - Biochemistry 2022Quote: ... A high-titer virus stock for hPolεCD(insect) was obtained by using the Bac-to-Bac Baculovirus Expression System from Invitrogen. 1.8×109 Sf21 cells in 1 L shaking culture were infected with the recombinant virus at a multiplicity of infection of 2 and cultivated at 27 ºC for 56 hr ...
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bioRxiv - Microbiology 2022Quote: ... Immunostaining of NS1 and TRIM25 was realized by using the following antibody combination: mouse anti-NS1/goat anti-mouse Alexa 488 (ThermoFisher Scientific) and rabbit anti-TRIM25 (abcam)/goat anti-rabbit Atto647N (sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... and High-Five™ insect cells (ThermoFisher Scientific) were cultured in ESF 921 serum-free medium (Expression Systems ...
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bioRxiv - Microbiology 2021Quote: ... High Five™ insect cells (Thermo Fisher Scientific) were maintained in rotating suspension cultures as recommended (Thermo).
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bioRxiv - Biochemistry 2024Quote: ... Spodoptera frugiperda Sf9 insect cells (Thermo Fisher Scientific) were infected with the virus at a cell density of 4.0 × 106 cells per milliliter in Sf900 II medium (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... High Five insect cells (B85502, ThermoFisher; 1.2–2.4 ×108 cells) were co-transfected with the recombinant viruses of appropriate combinations and were grown at 28°C for 60-72 hr.
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bioRxiv - Immunology 2023Quote: SF9 insect cells were maintained in HyClone SFX-Insect Cell Media (Cytiva) supplemented with 1X antibiotic-antimycotic (Thermo Scientific, Cat# 15240096) at 27°C with constant shaking at 100 RPM ...
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bioRxiv - Zoology 2024Quote: ... Insect cells medium was also supplemented with 1 % non-essential amino acid (Gibco, France). Mosquito cells were grown at 28°C and mammalian cells at 37°C ...
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bioRxiv - Cell Biology 2022Quote: His-CLASP1 protein was expressed in baculovirus-infected Sf9 insect cells using the Bac-to-Bac system (Invitrogen). After the first amplification ...