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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Chikungunya Virus VLP E1 E2 C protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The virus was rescued and propagated in T-REx-293 cells (Invitrogen). Purification was by CsCl gradient ultracentrifugation ...
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bioRxiv - Cancer Biology 2020Quote: ... Concentrated virus was filtered using 0.45um syringe filters (ThermoFisher; Cat#: 725-2545) and immediately added to cultured OSE in 24-well plates ...
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bioRxiv - Microbiology 2020Quote: ... Stocks of virus were grown in Vero-E6 cells in DMEM (Gibco) supplemented with 10% fetal bovine serum (Atlas Biologicals ...
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bioRxiv - Microbiology 2020Quote: ... containing 7,5 μl 4xTaqMan Fast Virus 1-Step Master Mix (Life Technologies) and 1 μl Primer/Probe mixture29,30 ...
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bioRxiv - Microbiology 2020Quote: ... the virus particles were resuspended in CO2-independent media (ThermoFisher, Cat# 18045088), aliquoted ...
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bioRxiv - Immunology 2019Quote: ... purified virus was immobilized on 96-well maxisorp microtiter plates overnight (Nunc). Wells were blocked with 0.05% PBST [0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... The virus was rescued and propagated in T-REx-293 cells (Invitrogen). Purification was by CsCl gradient ultracentrifugation ...
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bioRxiv - Immunology 2021Quote: ... Vero cells were used to propagate the virus in DMEM medium (Gibco) supplemented with 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... Virus was removed from cells and replaced with post-infection media (Gibco OptiMem supplemented with 0.01% FBS ...
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bioRxiv - Biophysics 2022Quote: ... X-31 influenza virus was diluted in Opti-MEM (Gibco, cat # 11058021) at a ratio of 1:10000 to add 250 µL into each well (25) ...
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bioRxiv - Microbiology 2022Quote: ... the reactions with Taqman Fast Virus 1-step master mix (ThermoFisher scientific) were half of the recommended 20 μl volume ...
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bioRxiv - Neuroscience 2023Quote: ... The virus-containing pellet was resuspended in 200 μL of DPBS (ThermoFisher) and stored at -80 °C ...
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bioRxiv - Microbiology 2023Quote: ... 10-fold dilutions of each virus in DMEM + 1% antibiotic/antimycotic (Gibco) were added to a monolayer Vero E6-TMPRSS2-T2A-ACE2 cells for 1 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Each virus was mixed with PBS-washed defibrinated sheep blood (Fisher Scientific) containing 5 mM ATP to a concentration of 106 PFU/mL and fed to ∼50-60 female mosquitoes ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis was performed with Moloney murine leukemia virus reverse transcriptase (Invitrogen) at 38°C for 60 min ...
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bioRxiv - Genomics 2019Quote: ... Proteins (20µg) were heated at 95°C with β-mercaptoethanol and then separated by SDS PAGE (Invitrogen). Nitrocellulose membranes were blocked in 5% nonfat milk in PBST for 1 hr and then incubated in primary antibody overnight at 4ºC ...
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bioRxiv - Biochemistry 2019Quote: ... 4°C and lysed using 400 µL B-PER bacterial protein extraction reagent (Thermo Scientific, MA, USA) according to the instructions of the manufacturer ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated at 4°C for 30-60 minutes with Protein A Dynabeads (Thermo Fisher Scientific). The beads were washed five times in wash buffer (20 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... 4 °C) and the protein concentration of the supernatant was determined using a BCA assay (ThermoFisher 23225). Samples were denatured (70% sample ...
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bioRxiv - Microbiology 2022Quote: ... Protein lysates were boiled at 95 °C for 5 mins in 6x SDS sample buffer (Thermo Scientific). Proteins were separated on 4-12% Bis-Tris SDS-PAGE gels (Thermo Fisher ...
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bioRxiv - Developmental Biology 2022Quote: ... at 95°C for 5 min and separated using NuPAGE 4%–12% Bis-Tris Protein Gel (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was denatured at 70 °C for 10 min with LDS Sample Buffer (Invitrogen, Cat#: NP0007) and Sample Reducing Agent (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: The purified JEV structural proteins (C, M, E, ED III) from the S2 insect expression system (Invitrogen) were quantified by using the bicinchoninic acid (BCA ...
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bioRxiv - Biochemistry 2021Quote: ... 0.03% NP40) and proteins were eluted by incubation in 1x LDS-sample buffer (Thermofisher, c/n NP0007) at 65°C for 10 minutes ...
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bioRxiv - Microbiology 2021Quote: ... sheared chromatin was precleared for 20 min at 4’C using Protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitations were performed using fresh beads and anti-Histone H3 acetyl K27 (H3K27Ac ...
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bioRxiv - Molecular Biology 2022Quote: ... and stored at −30°C before separation on 4-12% NuPAGE Bis-Tris protein gels (ThermoFisher Scientific).
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bioRxiv - Immunology 2022Quote: Recombinant tri-S protein was heat-denatured at 100°C for 3 min in loading buffer (Invitrogen) containing 1X sample reducing agent (Invitrogen) ...
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bioRxiv - Molecular Biology 2019Quote: ... Immunoprecipitation was performed at 4°C for 3 hr with 50 µl protein G dynabeads (Thermo Fisher). Beads were washed five times with ice-cold Wash buffer 1 (50 mM Hepes-KOH (pH7.6) ...
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bioRxiv - Microbiology 2020Quote: Proteins with C-terminal biotin tags were attached to streptavidin-coated dynabeads (Dynabead M-280 Streptavidin, Invitrogen) following standard procedures ...
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bioRxiv - Genetics 2020Quote: ... Proteins were denatured for 10 min at 70°C in NuPAGE LDS Sample Buffer (4X) (Invitrogen; NP0007) and NuPAGE Sample Reducing Agent (10X ...
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bioRxiv - Physiology 2021Quote: ... The total protein was inoculated with anti-HA magnetic beads at 4 C (Thermo Scientific, Rockford, IL) overnight ...
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bioRxiv - Immunology 2022Quote: ... STAT1 was immunoprecipitated by incubating for five hours at 4°C with Protein-G DynaBeads (Invitrogen #10004D). DNA bound to STAT1 complexes was extracted using 1% SDS and 0.6 mg/ml of Proteinase K (New England Biolabs #P81075 ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were denatured at 90 °C for 10 min with 10× reducing agent (Thermo Fisher Scientific) before gel electrophoresis ...
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bioRxiv - Immunology 2023Quote: ... sheared chromatin was precleared for 20 min at 4°C using Protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitations were performed using fresh beads and anti-Histone H3 acetyl K9 (H3K9Ac ...
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bioRxiv - Microbiology 2023Quote: ... before being incubated overnight at 4 degrees C with the antibodies bound to Protein A Dynabeads (Invitrogen). Samples were washed for 3 minutes each at 4 degrees C with low salt wash (0.01% SDS ...
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bioRxiv - Biochemistry 2022Quote: ... Target protein was coated overnight (O/N) at 4 °C on MaxiSorp™ 96-well plates (Nunc). ELISA-blocking buffer (PBS containing 0.5% (w/v ...
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bioRxiv - Developmental Biology 2023Quote: ... at 95°C for 5 min and separated using NuPAGE 4%–12% Bis-Tris Protein Gel (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen) ...
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bioRxiv - Plant Biology 2023Quote: ... The protein samples were denatured at 65 °C in the 1X LDS sample buffer (Invitrogen, Waltham, MA) with 2.5% β-mercaptoethanol for 5 minutes ...
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bioRxiv - Biophysics 2024Quote: ... precleared and immunoprecipitated overnight at 4 °C with 50 μL of GFP-conjugated Dynabeads Protein A (Invitrogen). Beads had previously been conjugated with 10 μL anti-GFP antibody (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... 4°C at 20817 x g and protein concentrations were determined by BCA assay (Thermo Fisher, 23225). Cell lysates (10 μg for HEK293E cells or 15 μg for MEFs ...
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bioRxiv - Biophysics 2024Quote: ... precleared and immunoprecipitated overnight at 4 °C with 50 μL of GFP-conjugated Dynabeads Protein A (Invitrogen). Beads had previously been conjugated with 5 μL anti-GFP antibody (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were stored at −80 °C or directly used for the quantification of total protein concentrations with the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, United States). Thereafter ...
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bioRxiv - Microbiology 2020Quote: ... supernatant was harvested for virus titration and cells were lysed in Trizol (Invitrogen). MHV in the supernatant was quantified by standard plaque assay on L2 cells.
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bioRxiv - Cell Biology 2020Quote: ... HEK-293T cells used for virus production were cultured in IMDM (Life Technologies) supplemented with 20% IFS ...
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bioRxiv - Neuroscience 2020Quote: ... Virus-containing supernatant was harvested three days after transfection with Lipofectamine 2000 (Thermofisher). This supernatant was then cleared from cell debris by centrifugation at 3500 rpm for 15 min and filtered through a 0.45 µm filter (Millipore) ...
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bioRxiv - Neuroscience 2021Quote: ... to wash the virus in Opti-MEM™ Medium (ThermoFisher Scientific, Darmstadt, Germany) and concentrate to a final volume of 150 μl.
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bioRxiv - Neuroscience 2020Quote: ... They were reprogrammed into iPSCs using the replication incompetent Sendaï virus kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The virus pellet was then re-suspended in PBS (Thermo Fisher Scientific, 10010015) containing 25 mM HEPES buffer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... For this the virus was diluted in 3 mL of 199 medium (Gibco). After incubation for 2 h at 37°C the medium was aspirated and 3 mL of fresh DMEM (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... respectively) and 5 μl of 4X TaqMan Fast Virus 1-step mastermix (ThermoFisher). The qPCR plate was sealed and read in the FAM and HEX channels using a QuantStudio™ 5 Real-Time PCR system (ThermoFisher ...