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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Chikungunya Virus VLP E1 E2 C protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1:2 diluted whole cell extract in buffer C was added to Dynabeads Protein G (Thermo Fisher Scientific) pre-incubated with HEXIM1 antibody ...
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bioRxiv - Cell Biology 2023Quote: ... 4°C) and the protein concentration in cellular lysates was determined by absorbance on a nanodrop instrument (ThermoFisher). 1 mg of lysate in 1 mL of lysis buffer was incubated overnight at 4°C with 20 μL of anti-FLAG M2 magnetic bead slurry (MilliporeSigma ...
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bioRxiv - Cell Biology 2024Quote: ... were pre-bound overnight at 4°C to 60 μl of Dynabeads protein G (10003D, Thermo Fisher Scientific) diluted in 250 μl of blocking buffer (PBS ...
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bioRxiv - Microbiology 2024Quote: ... 4 °C) and protein concentration in the supernatant was determined by a commercially available BCA assay (ThermoFisher Scientific). The same protein amount was used for each measurement.
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bioRxiv - Plant Biology 2024Quote: ... The EIN2-C native antibody was prebound with the equilibrated protein-G coated magnetic beads (Dynabeads, Thermo Fisher) with 1% BSA for 3 h with gentle rotation at 4 °C and incubated at 4 °C after cleared cell fractionation addition with gentle rocking overnight for Co-IP ...
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bioRxiv - Molecular Biology 2024Quote: ... FLAG M2 or HA-tag antibody at 4 °C overnight before precipitated with magnetic Protein G beads (Thermofisher) for 2 hours at 4 °C followed by 3 washes with RIPA buffer.
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bioRxiv - Molecular Biology 2024Quote: ... Immune complexes were pulled down for 45 min at 4°C with 25 µL protein G dynabeads (Invitrogen), captured on a magnet and washed 4 times in 800 µL IP buffer ...
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bioRxiv - Cell Biology 2024Quote: ... protein samples were denatured at 70°C for 10 min in LDS Sample Buffer (Thermo Fisher Scientific, NP0007) with or without 100 mM Dithiothreitol (+DTT and –DTT ...
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bioRxiv - Cell Biology 2024Quote: LZTR1WT and LZTR1L580P were expressed as C-terminal His-tagged proteins in Expi-293F cells (Thermo Fisher Scientific). pcDNA3.1-LZTR1-Myc-6xHis plasmid was a gift from Jens Kroll (Heidelberg University and German Cancer Research Center (DKFZ-ZMBH Alliance)).56 The LZTR1L580Pvariant was introduced into the plasmid by site-directed mutagenesis as previously described.11 Cells were transfected using ExpiFectamine 293 Reagent (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... qPCR was performed using TaqMan Fast virus 1 Step PCR Master Mix (Life Technologies), standard curve was drawn with 2019_nCOV_N Positive control (IDT) ...
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bioRxiv - Immunology 2022Quote: ... amplified using the TaqMan Fast Virus 1-Step Master Mix qRT-PCR kit (Invitrogen) on a LightCycler 480 or LC96 instrument (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... and the virus was produced in HEK293A cells as described by the manufacturer (Invitrogen). Immature brown adipocytes were treated with the viruses on day 2.
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bioRxiv - Microbiology 2019Quote: ... probes and TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems, Foster City, CA). We used pre-combined probe and primers (500 nM primers and 250 nM probe ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed using Moloney murine leukemia virus reverse transcriptase (Ambion-ThermoFisher Scientific) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies) ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed using Moloney murine leukemia virus reverse transcriptase (Ambion-ThermoFisher Scientific) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 or 3 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... while 4x Taqman Fast Virus 1-Step Master Mix (Life Technologies, Carlsbad, CA, USA) was used for RNA enteric viruses ...
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bioRxiv - Immunology 2021Quote: ... then incubated with the appropriate amount of virus diluted in Fluorobrite DMEM (Life Technologies) medium supplemented with 2% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RNA was quantified using qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2021Quote: For Sendai virus iPSC reprogramming using CytoTune™-iPS 2.0 Sendai Reprogramming kit (Invitrogen), fibroblasts were reprogrammed as previously described (Milagre et al. ...
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bioRxiv - Immunology 2022Quote: ... RNA from virus stocks was purified using TRIzol Reagent (ThermoFisher Scientific, catalog no. 15596026). Brief ...
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bioRxiv - Immunology 2022Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region and gene-specific probes and reverse primers as follows:
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bioRxiv - Microbiology 2022Quote: ... using TaqMan Fast Virus 1-Step Master Mix (Cat. n° 4444434, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The cDNAs were synthesized using random primers and Moloney murine leukemia virus RT (Invitrogen). The RT-PCR products were separated on a 2% w/v agarose-TBE gel ...
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bioRxiv - Neuroscience 2020Quote: ... Fibroblasts were reprogrammed into iPSCs by using the replication-incompetent Sendai virus kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... were quantified by RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) and specific primers and probes (Supplementary Table 3) ...
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bioRxiv - Cancer Biology 2021Quote: ... Isolated RNA was reverse transcribed with Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen) and oligo(dT ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from mock or virus infected cells using Trizol reagent (Invitrogen) at 2 and 4 hours post infection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... UKEi001-A was reprogrammed by a Sendai Virus (CytoTune iPS Sendai Reprogramming Kit, ThermoFisher). EHT patch casting was performed by mixing CMs and a fibrinogen/thrombin mix3 ...
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bioRxiv - Immunology 2019Quote: ... and inoculated with virus at the optimal MOI of 100:1 in DMEM (Gibco). After 1.5 h ...
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bioRxiv - Microbiology 2020Quote: ... serial dilutions of virus stocks in Minimum Essential Media MEM medium (Gibco, #41500-018) supplemented with 2% FBS was added to Vero E6 monolayers on 24-well plates (Greiner bio-one ...
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bioRxiv - Zoology 2020Quote: ... 6.25μL of 4X RT-Buffer (TaqMan Fast Virus 1-Step Master Mix, ThermoFisher Scientific), 600nM of the forward primer (RdRp_SARSr-F) ...
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bioRxiv - Genetics 2021Quote: ... RNA was reverse transcribed using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific) for 1 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Eluted RNA was coupled with TaqMan Fast Virus 1-step Master Mix (Applied Biosystems) and nCOV_N1 primers/probe (IDT ...
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bioRxiv - Cell Biology 2020Quote: ... virus particles were then purified by gel filtration (7K molecular weight cut-off, Invitrogen).
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bioRxiv - Immunology 2021Quote: ... total RNA was reverse-transcribed with Moloney murine leukemia virus reverse transcriptase (Fisher Scientific) using random hexamers (Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... prior to addition of PE243 virus in complete DMEM media +10 mM HEPES (Gibco), at a multiplicity of infection (MOI ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: UKEi001-A was reprogrammed with a Sendai Virus (CytoTune iPS Sendai Reprogramming Kit, ThermoFisher). Expansion and cardiac differentiation of iPSC were performed as recently described1 ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-Influenza A Virus M1 (1:1,000; clone: GA2B; Cat#: MA1-80736; Invitrogen), mouse anti-Influenza A virus M2 (1:1,000 ...
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bioRxiv - Bioengineering 2022Quote: ... consisting of 5 μL TaqMan Fast Virus 1-Step Master Mix (cat# 4444432, Thermofisher), 1.2 μL forward primer (0.6 μm) ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-Influenza A virus M2 (1:1,000; clone: 14C2; Cat#: MA1-082; Invitrogen), mouse anti-GAPDH (1:1,000 ...
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bioRxiv - Neuroscience 2022Quote: ... Virus (undiluted) or FluoSpheres (580/605, 0.04 µm, diluted to 20% in saline, Invitrogen) were loaded into freshly pulled glass micropipettes and injected using a Nanoject III pressure injector (Drummond Scientific Company ...
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bioRxiv - Microbiology 2022Quote: ... RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 3 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2022Quote: ... Virus amplification was carried out in Spodoptera frugiperda Sf9 cells (Geneart, Thermo Fisher Scientific) and was used to infect Trichoplusia ni Tnao38 cells (103 ...
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bioRxiv - Immunology 2022Quote: ... Reverse transcription was performed using Murine Moloney Leukemia Virus Reverse Transcriptase (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... medium containing virus isolates was incubated in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 11995073) with 1:10000 Vybrant (V22887 ...
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bioRxiv - Immunology 2023Quote: ... Concentrated virus was then incubated using 1:2000 DiD-Cell labelling solution (ThermoFisher V22887) for 20 mins at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific) and real-time PCR was performed using TaqMan assays (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Cellular RNA was mixed with TaqMan Fast Virus 1-step Master Mix (Applied Biosystems), 10 mM forward and reverse primers and 2 mM probe ...