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Citations for Thermo Fisher :
651 - 700 of 6914 citations for CD28 Human Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: 6x His tag monoclonal antibody (Invitrogen) or streptavidin (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... + 10% HI-Horse Serum (Gibco, 26050088) + 0.8% Pen/Strep (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... (hi-FCS, Gibco, Life Technologies, UK), were incubated (37°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... (hi-FCS, Gibco, Life Technologies, UK), were incubated (37°C ...
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bioRxiv - Microbiology 2021Quote: Plasmid based on pBAD/His (Invitrogen) or pHT01 (MoBiTech ...
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bioRxiv - Microbiology 2020Quote: ... the pIB/V5-His vector (Invitrogen) and insert egfp fragment were amplified by PCR ...
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bioRxiv - Biochemistry 2020Quote: ... Anti‐His tag monoclonal antibody (Invitrogen) was used in western blot analysis.
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bioRxiv - Biochemistry 2021Quote: Modified pBAD/His B vector (Invitrogen) containing native dnrK and tamK31,39 were used as templates for chimeragenesis ...
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bioRxiv - Biochemistry 2021Quote: ... His (1:1000, Invitrogen, mouse monoclonal), Flag M2(1:1000 ...
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bioRxiv - Immunology 2022Quote: ... and Hi-5 cells cells (Invitrogen), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... + 10% HI-FBS (Thermo Fisher, 16140071) prior to adding indicated concentration of human IFN-alpha 2b (pbl Assay Science ...
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bioRxiv - Microbiology 2020Quote: ... His tagged proteins were isolated from the supernatant using Dynabeads™ His-Tag Isolation and Pulldown (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The His-tagged proteins were blotted using anti-6x His tag HRP-conjugated monoclonal antibody (Invitrogen, USA) and detected using Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... purified memory T cells were stimulated with anti-CD3/CD28 Dynabeads (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated with anti-CD3/CD28 magnetic dynabeads (Thermo Fisher) at a bead to cell concentration of 1:1 ...
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bioRxiv - Cancer Biology 2019Quote: Naïve T cells were activated with CD3/CD28 beads from Thermo Fisher Scientific (Catalog no ...
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bioRxiv - Bioengineering 2022Quote: CD8+ T cells were activated using anti-CD3/CD28 coated beads (Invitrogen) at a 1:2 ratio (beads:cells ...
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bioRxiv - Immunology 2020Quote: ... or 0.5μg/mL immobilised anti-CD3 and 5μg/mL anti-CD28 (ThermoFisher) (centre B) ...
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bioRxiv - Cancer Biology 2021Quote: CD8+T cells were stimulated using anti-CD3/CD28 Dynabeads (Life Technologies) in complete T cell media and under normal growth conditions for 48-72 hours prior to electroporation ...
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bioRxiv - Immunology 2020Quote: ... 2×106 cells were activated anti-CD3/CD28 beads (Thermo Fisher Scientific) in a 4:1 ratio of cells:beads for 72 hrs at 37°C ...
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bioRxiv - Immunology 2020Quote: ... and 1μg/mL soluble anti-CD28 (eBioscience™, ThermoFisher Scientific, Clone: 37.51) antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μg/mL anti-CD28 antibody (ThermoFisher Scientific, Cat#16-0289-85), 100 ng IL-2 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Cells were activated using Dynabeads Mouse T-Activator CD3/CD28 (Gibco #11452D) at a ratio of 1:1 cells to beads in culture for 72 hours prior to assessment.
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bioRxiv - Synthetic Biology 2022Quote: ... For cells stimulated with anti-CD3/anti-CD28 antibody coated DynaBeads (Gibco), beads were added to cells in a 3:1 ratio ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells were activated using anti-CD3/CD28 coated beads (Invitrogen) at a 1:2 ratio (beads:cells ...
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bioRxiv - Immunology 2022Quote: ... T cells were stimulated with Dynabeads CD3/CD28 CTS (Thermo Fisher Scientific) at a 1:1 bead ...
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bioRxiv - Immunology 2022Quote: ... These cells are then stimulated with anti-CD3/CD28 beads (Gibco #11456D) and coculture with splenic MDSC isolated using Stemcell EasySep™ Mouse Isolation kit (Stemcell #19867 ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were activated with CD3/CD28 Dynabeads® (Thermo Fisher Scientific) and stimulated with 80 U/ml IL-2 (Peprotech® ...
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bioRxiv - Systems Biology 2023Quote: ... The naïve/memory T cells were stimulated with CD3/CD28 Dynabeads (ThermoFisher) at a 3:1 cell-to-bead ratio for one week ...
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bioRxiv - Immunology 2023Quote: ... and stimulated with anti-CD3/CD28 Dynabeads (Thermo Fisher Scientific, Cat#40203D). T-cells were cultured in X-VIVO medium with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... stimulated with CD3/CD28 T-cell activation Dynabeads (Life Technologies, Carlsbad, USA) at a 1:1 bead to cell ratio ...
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bioRxiv - Immunology 2023Quote: ... TCR stimulation was induced by adding anti-CD3/CD28 Dynabeads (Applied Biosystems) at the concentration of 2µL per 80,000 cells (1:1 ratio of cells:beads) ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 0.5 μg/mL soluble anti-CD28 (clone 37.51, Invitrogen, Waltham, MA, USA), 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 0.5 μg/mL soluble anti-CD28 (clone 37.51, Invitrogen, Waltham, MA, USA), 37°C and 5% CO2 for 48 hours in RPMI 1640 containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... and activated with anti-CD3/CD28 Dynabeads (Thermo Fisher Scientific, Waltham, MA) at a bead:cell ratio of 1:3 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 5 μg/ ml anti-CD28 (Thermo Fisher Scientific, 16-0281-82) antibodies were added to 2×106 T cells/ ml in RPMI medium ...
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bioRxiv - Genetics 2024Quote: ... Then isolated T cells were incubated by CD3/CD28 Dynabeads (Thermofisher # 11131D) for T Cell expansion and activation for 48 hours ...
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bioRxiv - Immunology 2024Quote: ... T cells were activated with anti-CD3/CD28 beads (Gibco, ThermoFisher Scientific) at a 1:1 ratio and recombinant human IL-2 (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... T cells were activated with anti-CD3/CD28 beads (Gibco, ThermoFisher Scientific) at a 1:1 ratio and recombinant human IL-2 (Peprotech ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were then stimulated with 25 ng/ml EGF (Gibco) for 5 min (other cell lines were not stimulated) ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) supplemented with 10% (v/v ...
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A nepenthesin insert allosterically controls catalysis in the malaria parasite protease plasmepsin VbioRxiv - Microbiology 2022Quote: ... HEK293-F cells were cultured in FreeStyle 293 Expression Medium (ThermoFisher). A days before transfection ...
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bioRxiv - Cell Biology 2019Quote: ... Transient transfection of HEK293 cells was carried out using Lipofectamine2000 (ThermoFisher) after cells reach a confluency of 70%.
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bioRxiv - Neuroscience 2020Quote: ... HEK293 cells were cultured with Dulbecco’s Modified Eagles Media (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: HEK293 and COS-7 cells were transfected with Lipofectamine 2000 (Invitrogen) according to manufacturer’s specifications ...
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bioRxiv - Biochemistry 2020Quote: HEK293T (ATCC) and HEK293 (ATCC) cells were cultured in DMEM (Invitrogen) plus 10% FBS (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... Transfections of HEK293 were performed with 4 μL Lipofectamine 2000 (Invitrogen) and 1-2.5 μg plasmid DNA per 35 mm2 ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 cells were maintained in Eagle’s Minimum Essential Medium (EMEM; Gibco) and SH-SY5Y cells in Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 (DMEM/F12 ...
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bioRxiv - Immunology 2021Quote: ... HEK293-hCD4 were detached using enzyme-free cell dissociation buffer (Gibco) and resuspended in FACS buffer (PBS containing 5% FBS) ...
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bioRxiv - Molecular Biology 2020Quote: HEK293 (HEK) cells were grown in DMEM Media (Thermo Fisher Scientific) supplemented with 1mM Glutamax (Thermo Fisher Scientific) ...