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Citations for Thermo Fisher :
451 - 500 of 6914 citations for CD28 Human Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Dynabeads™ Mouse T-Activator CD3/CD28 (ThermoFisher 11453D) were used to activate cells at a 1:4 bead-to-cell ratio for 24h before transduction ...
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bioRxiv - Immunology 2021Quote: ... 5 µL/mL of anti-CD3/CD28 Dynabeads (Invitrogen) were added to each well ...
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bioRxiv - Biochemistry 2022Quote: ... and activated with CD3/CD28 Dynabeads (Life Technologies 11132D) in supplemented CTS OpTmizer (Life Technologies A1048501 ...
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bioRxiv - Immunology 2022Quote: ... cells were activated with CD3/CD28 Activator Beads (Gibco). After 2 days beads were magnetically removed and cells re-plated without changing media or adjusting cell number ...
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bioRxiv - Immunology 2022Quote: ... and stimulated with anti-CD3/anti-CD28 Dynabeads (Invitrogen) at a bead:cell ratio of 1:10 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 250,000 CD3/CD28 Dynabeads (Thermo Fisher Scientific, Cat: #11132D) were added to each well ...
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bioRxiv - Cell Biology 2023Quote: ... CD3/CD28 Dynabeads (1 bead per cell, Thermofisher, Europe) or Phytohemagglutinin (10 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... activated with anti-CD3/CD28 beads (Thermo Fisher Scientific) in a ratio of 1:1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Stimulation was provided by anti-CD3/CD28 Dynabeads (Invitrogen), as well as anti-human PD-1 (clone pembrolizumab ...
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bioRxiv - Immunology 2023Quote: ... and Dynabeads Mouse T-activator CD3/CD28 (Thermo Fisher) (8.3 CD8 T:beads = 1:1 ...
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bioRxiv - Molecular Biology 2023Quote: ... and activated by CD3-CD28 Dynabeads (ThermoFisher Scientific, UK). After incubating at 37°C/ 5% CO2 for three days ...
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bioRxiv - Immunology 2023Quote: ... Dynabeads Mouse T-Activator CD3/CD28 (11452D, ThermoFisher Scientific) at a 1:1 cell:bead ratio ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated with anti-CD3ε/CD28 (ThermoFisher #11453D) beads at indicated beads to cell ratios for 16 hours ...
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bioRxiv - Immunology 2023Quote: ... Anti-CD28 (final concentration 2 ug/mL, Life technologies) and recombinant human IL-2 (final concentration 50 U/mL ...
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bioRxiv - Immunology 2024Quote: ... anti-CD3/CD28 T-cell activating DynaBeads (Thermo scientific) were magnetically washed in 1mL DPBS and added to T-cells at a 1:1 ratio ...
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bioRxiv - Immunology 2023Quote: Human-derived cell lines used in this study were initially obtained from accredited commercial suppliers: (1) HEK293 (human embryonic kidney cell line, FreeStyle® 293-F) was obtained from Invitrogen (San Diego, CA) for use as a recombinant protein expression vehicle ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). Prior to analyses ...
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bioRxiv - Immunology 2023Quote: ... were seeded in Ultraculture complete medium and partially activated with dynabeads human T-activator CD3/CD28 (final dilution 1:1000) (Thermo Fisher Scientific, Waltham, MA USA) or completely activated with dynabeads human T-activator CD3/CD28 and IL2 2.5 U/ml ...
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bioRxiv - Genomics 2019Quote: ... 500 ng of in situ Hi-C libraries were first mixed with 2.5 μg human Cot-1 DNA (Invitrogen #15279011), 2.5 μg salmon sperm DNA (Invitrogen #15632011) ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
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bioRxiv - Immunology 2021Quote: ... The expression vectors encoding mHVEM (Q39-Q206) fused with human IgG1 and a subsequent hexa-His tag sequences were transfected into Expi293 (Gibco) cells using ExpiFectamine 293 transfection kit (Gibco ...
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bioRxiv - Immunology 2021Quote: ... The DNA sequences encoding a protein biologic composed of mHVEM residues (Q39- Q206) followed by human IgG1 and a subsequent hexa-His tag sequences were cloned into pcDNA 3.3 vector (Life technologies) using In-fusion HD cloning enzyme premix (Clontech) ...
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Microbiology 2020Quote: ... Tsp-his was detected with the Anti-6X His tag antibody (ThermoFisher Scientific), which is already conjugated with HRP and does not require a secondary antibody for detection ...
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bioRxiv - Developmental Biology 2023Quote: ... For antibodies we used a His antibody (6x-His Tag Monoclonal Antibody, ThermoFisher), a MBP antibody (Anti-MBP Monoclonal Antibody ...
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bioRxiv - Biophysics 2020Quote: The DNA sequences of the human CE GTase and each sequence variant were synthesised and subcloned into the PGEX6p1-C-His plasmid vector by Thermo Fisher GeneArt ...
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bioRxiv - Neuroscience 2021Quote: His-tagged human dynamin 1 was expressed using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific, Waltham, MA, USA) and purified as described previously (66) ...
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bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
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bioRxiv - Physiology 2021Quote: ... The human full-length BACE1 or BACE2 cDNAs were expressed from the pcDNA3.1/myc-His expression vector (Invitrogen, Waltham, MA, USA).
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bioRxiv - Biophysics 2021Quote: ... HEK293S GnTl- cells were cultured in Freestyle 293 (GIBCO) supplemented with 2% FBS and 1% Antibiotic-Antimycotic ...
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bioRxiv - Evolutionary Biology 2021Quote: ... HEK293 cells were cultured in DMEM medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293 cells were cultured in DMEM medium (Gibco/BRL) supplemented with 10% (v/v ...
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bioRxiv - Genetics 2019Quote: HEK293 cells were maintained in DMEM plus Glutamax (Gibco) supplemented with 10% heat-inactivated FBS in a humidified 37°C ...
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bioRxiv - Immunology 2021Quote: ... Lentiviral particles were produced in HEK293-FT cells (Invitrogen). Then ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were expressed in HEK293 Freestyle cells (Invitrogen) in suspension culture using serum-free media (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... We cultured HEK293 cells in DMEM medium (Gibco, #11965092) with 10% fetal bovine serum and 1% penicillin– streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... transfected with 293fectin into FreeStyle HEK293-F cells (Invitrogen), and purified from culture supernatants by sequential HisTrap Ni2+-NTA and Superdex 200 columns (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: HeLa cells (ATCC CCL-2) and HEK293-FT (ThermoFisher) were cultured at 37 °C in 5% CO2 in medium composed of high glucose Dulbecco’s modified eagles medium (DMEM ...
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bioRxiv - Synthetic Biology 2021Quote: HEK293 cells were cultured in DMEM (Gibco; Life Technologies) with 10% FBS (Gibco ...
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bioRxiv - Synthetic Biology 2021Quote: HEK293 cells were cultured in DMEM (Gibco; Life Technologies) with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2019Quote: HEK293 Flp-In™ T-Rex™ cells (Invitrogen) or HEK293T cells (ATCC ...
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bioRxiv - Genomics 2021Quote: HEK293 cells (ATCC) were cultured in DMEM (ThermoFisher Scientific) with 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Cell Biology 2021Quote: ... Polyclonal HEK293 Flp-In T-REx cell lines (Invitrogen) were generated as described previously (Wyler et al. ...
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bioRxiv - Molecular Biology 2020Quote: HEK293 cells were dissociated with TrypLE (Thermo Fisher Scientific), washed ...
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bioRxiv - Biophysics 2020Quote: HEK293 cells were transfected using Lipofectamine 3000 (Thermo Fisher). SERCA2a constructs were expressed using the mammalian expression vector pcDNA3.1 with G418 resistance ...
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bioRxiv - Cell Biology 2020Quote: ... Flp-In T-Rex HEK293 (R78007, Thermo Fisher Scientific) and HaCaT (obtained from Joan Massague’s lab at Memorial Sloan Kettering Cancer Centre ...
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bioRxiv - Biophysics 2021Quote: ... HEK293S GnTI- cells cultured in Freestyle 293 medium (GIBCO) supplemented with 2% FBS at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... for HEK293 FT cells and Lipofectamine 2000 (Thermo Fisher) for fibroblast cell lines ...
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bioRxiv - Molecular Biology 2020Quote: HeLa S3 and HEK293 T-REx (Thermo Fisher Scientific) cells were maintained in DMEM supplemented with 10% FBS in a humidified incubator at 5% CO2 and 37°C ...
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bioRxiv - Genomics 2021Quote: HEK293 LTV cells were cultured in DMEM medium (Gibco), supplemented with 10% FBS at 37°C ...