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Citations for Thermo Fisher :
301 - 329 of 329 citations for Brucella Abortus Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and anti-TRIP4 antibodies were raised in rabbits against affinity purified N-terminally hexahistidine-tagged full-length proteins and purified with the respective antigens coupled to SulfoLink resin (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... Washed yeast libraries were stained in dark and at 4°C with biotinylated antigens pre-conjugated with SA-PE (Streptavidin, R-Phycoerythrin Conjugate, Thermo Fisher Scientific) or SA-APC (Streptavidin ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 antigen sequences were cloned into pcDNA3.4 plasmids and transfected into 293F cells utilizing the Expi293 Expression System (Life Technologies, Cat # A14635) per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight with either Dengue 2 fixed virus antigen (Microbix, Cat# EL-22-02) or polyvalent total immunoglobulin (Invitrogen, Cat# H17000). Before adding PBMC’s ...
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bioRxiv - Immunology 2020Quote: ... the RBD antigen (diluted to 2 μg/ml in PBS) was first adsorbed to 96-well clear Immulon 4 HBX (Thermo Scientific, #3855) plates in PBS overnight at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... A33 and B5 antigens were coupled via carboxyl chemistry to Magplex® fluorescently bar-coded beads (Luminex Corporation) with sulfo-NHS and EDC (Thermo Fisher) per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and masked epitopes were recovered with eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Invitrogen, Waltham, MA, USA, #00-4956-58). Slides were blocked for 1 hour at room temperature with blocking solution (1xPBS ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently stained for viability and surface antigens and then intracellularly stained using the eBioscience Intracellular Fixation and Permeabilization Kit (Thermo Fisher Scientific) using the antibodies and reagents listed in Supplemental Table 5.
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bioRxiv - Pathology 2021Quote: ... for 1 week and pepsin (1 mg/ml in 0.1N HCl) was used for antigen retrieval for immunostaining with human COMP (Thermofisher; MA1-20221, 1:100), CHOP (Santa Cruz ...
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bioRxiv - Molecular Biology 2020Quote: ... a female human embryonic kidney cell line transformed with the SV40 large T antigen was obtained from ATCC (#CRL-1573) and cultured in complete media consisting of DMEM (#11965092, GIBCO, Life Technologies; Carlsbad, CA) supplemented with 10% FBS (Seradigm Premium Grade HI FBS ...
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bioRxiv - Microbiology 2020Quote: ELISA was carried out to estimate the avidity and overall stability of antigen-antibody complexes using the chaotropic agent ammonium thiocyanate (NH4SCN) (Acros Organics Fisher Scientific). The plates were coated with 50 ng of PvRBP2b161-1454 in PBS and incubated overnight ...
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bioRxiv - Biophysics 2022Quote: ... We revealed the antigens coating with a Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor 647, Invitrogen, ref. A-21447) and analyzed the fluorescence intensity ...
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bioRxiv - Microbiology 2020Quote: Total RNA was extracted from cell pellets collected in QuantiFERON-TB gold assay tubes without antigen using the Ribopure Ambion RNA isolation kit (Life Technologies, California, USA), and eluted RNA treated with DNase for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... and adjusted tissue and plasma extracts were added with the antiserum against OT (1:1,000 dilution) to each antigen-coated well of a 96-well microplate (F96 maxisorp nunc-immuno plate;Thermo Fisher Scientific, Roskilde, Denmark) and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... for antigen retrieval in a pressure cooker for 10 minutes then sections were blocked in CAS-block histochemical reagent (Thermo Fisher Scientific, 008120). Permeabilization was performed with 0.5% Triton X-100 in PBS for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Both FFPE and OCT cell array samples were incubated for 5min at 100°C in 1x Antigen Retrieval Solution (ThermoFisher, 00-4956-58) using a pressure cooker ...
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bioRxiv - Pathology 2023Quote: Slides were incubated at 90°C for 20 minutes in High pH IHC Antigen Retrieval Solution (Thermo Fisher Scientific Catalog Number 00-4956) and allowed to cool to room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... by measuring the expression of pluripotency surface antigen (SSEA4 1:20, Invitrogen, 12-8843-42, and TRA1-60 1:20, Invitrogen, 12-8863-82) by flow cytometry ...
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bioRxiv - Molecular Biology 2020Quote: ... a female human embryonic kidney cell line transformed with the SV40 large T antigen was obtained from ATCC (#CRL-1573) and cultured in complete media consisting of DMEM (#11965092, GIBCO, Life Technologies; Carlsbad, CA) supplemented with 10% FBS (Seradigm Premium Grade HI FBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... rehydrated and underwent antigen unmasking by heat-induced epitope retrieval in 1× citrate buffer (pH 6.0; Thermo Scientific, Waltham, MA, USA #AP-9003-500). The sections were rinsed with PBS and blocked for 2 hours at room temperature using 10% normal donkey serum (NDS ...
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bioRxiv - Bioengineering 2020Quote: ... The specificity of the signals of antibodies against specific antigens was determined by performing a control experiment using compensation beads (Invitrogen, Cat# 01-1111-42).
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bioRxiv - Physiology 2020Quote: ... Antibodies to the antigen peptide were purified from the final bleed antiserum by affinity-purification using the AminoLink Plus Immobilization Kit (ThermoFisher Scientific, Waltham, MA, USA), with bound antibodies eluted using glycine elution buffer [6.3 ml of 100 mM glycine (VWR Chemicals ...
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bioRxiv - Immunology 2022Quote: ... Fluorescent antigen probes were used to detect VRC01gHL B cells with eOD-GT8 60-mer conjugated to Alexa Fluor 647 (Thermo Fisher Scientific, Catalog # A20173). Intracellular staining was performed with a fixation kit (BD Bioscience ...
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bioRxiv - Cell Biology 2023Quote: ... The solution was adjusted to 5 µg ml-1 in a total of 100 µl per well diluted antigen and were coated into Pierce™ Nickel Coated Plates (Thermo Fisher Scientific, #15142) at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... from singly sorted antigen-specific memory B cells were RT-PCR amplified using SuperScript III and AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Waltham, MA) under conditions previously described46.
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bioRxiv - Microbiology 2022Quote: Recombinant trimeric ectodomain of SARS-CoV-2 S protein with two proline mutations (rtS-ecto2P) as a vaccine antigen was produced using a Drosophila expression system (Thermo Fisher Scientific, Grand Island, NY). The protein sequence was modified to remove the furin cleavage site (RRAR to GSAG) ...
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bioRxiv - Immunology 2020Quote: ... then stained with surface and/or intracellular flow cytometry antibodies specific to antigens of interest using eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, Waltham, MA) according to manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: ... cells were stained following the ThermoFisher “BestProtocols: Staining Intracellular Antigens for Flow Cytometry – Protocol A” using the eBioscienceTM Permeabilization Buffer (ThermoFisher Scientific cat#: 00-8333-56) and eBioscienceTM IC Fixation Buffer (ThermoFisher Scientific cat# ...
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bioRxiv - Microbiology 2022Quote: ... Clones expressing different surface antigens were obtained by limiting dilution in 96-well culture plates placed in anaerobiosis chambers (Anaerogen® Compact, Thermo Scientific® Oxoid®, Cat. # AN0010C) at 37 °C during 5 days ...