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Citations for Thermo Fisher :
151 - 200 of 329 citations for Brucella Abortus Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Antigen-coupled microspheres were washed and incubated with plasma samples at an appropriate sample dilution (1:500 for IgG1 and all Fcγ-receptors ...
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bioRxiv - Molecular Biology 2020Quote: ... Antigen retrieval was done using 1X PT Module buffer solution (Thermo Scientific, Catalog#: TA-250-PM4x) and Decloaking Chamber Pro (Biocare Medical ...
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bioRxiv - Genomics 2021Quote: ... The sections were boiled in IHC Antigen Retrieval Solution (ThermoFisher Scientific; catalog number 00-4955-58) containing 0.05% Tween-20 for 10 minutes and then placed in PBS for 20 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Human embryonic kidney 293T cells containing SV40 large T-antigen (HEK293T) were maintained in DMEM (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). The antigen-coupled microspheres were washed and incubated with heat-inactivated serum samples at an appropriate sample dilution (1:100-1:400 for antibody isotyping and 1:1,000 for all low-affinity FcγRs ...
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bioRxiv - Biochemistry 2022Quote: ... A dilution series of the antigens was prepared in PEA buffer and 10% chicken serum (Invitrogen), and each dilution series included negative controls with no protein added to determine the assay background ...
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bioRxiv - Cancer Biology 2023Quote: ... Paraffin-embedded tumor sections were treated for antigen retrieval with sodium citrate buffer (Thermo Scientific Fisher). All tumor sections were blocked in blocking buffer containing PBS (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... the purified antigens were then biotinylated with the EZ-Link Sulfo-NHS-Biotin (Thermo Fisher Scientific) using a 50:1 biotin to protein molar ratio for calculations.
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bioRxiv - Biochemistry 2022Quote: ... and FL Spike trimers from hCoVs polypeptide antigens) were purified using HisPur™ Ni-NTA resin (ThermoFisher). Cell supernatants were diluted with 1/3rd volume wash buffer (20 mM imidazole ...
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bioRxiv - Biophysics 2022Quote: ... Antigen recognition was performed by incubating primary antibody in a humidified chamber for different times (Phalloidin, Invitrogen, A12381 ...
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bioRxiv - Biochemistry 2019Quote: ... a rabbit polyclonal antibody specific to soluble tachyzoite antigen (STAG) directly conjugated to FITC (PA1-7253, Invitrogen) was diluted 1:300 in TBS-T (0.1% Tween20 ...
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bioRxiv - Cell Biology 2020Quote: ... The antiserum was affinity purified using the antigen coupled to Dyna M-280 Streptavidin beads (Life Technologies).
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bioRxiv - Biochemistry 2020Quote: ... antigens or capture antibodies were coated in PBS overnight onto 384 microwell plates (Maxisorp, Thermo Fisher Scientific), washed 5 times with PBST (PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... the antigen was detected using chemiluminescence Western blotting detection reagents (Pierce ECL Western Blotting Substrate, Thermo Fisher Scientific Inc. ...
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bioRxiv - Pathology 2022Quote: ... Antigen antibody complexes were visualised with anti-mouse Alexa 488- or 594-conjugated antibody (Thermo Fisher Scientific) or Immpress anti-mouse/rabbit coupled with tyramide amplification kit (PerkinElmer ...
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bioRxiv - Plant Biology 2020Quote: ... The antibody-antigen complex was detected by using the SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Scientific) and the FujiFilm LAS-3000 or Amersham imager 600 analyzers.
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bioRxiv - Neuroscience 2019Quote: ... antibody/antigen mixture was incubated with Dynabeads M-280 sheep anti-rabbit IgG (Thermo Fisher Scientific, 11203D) or Dynabeads M-280 sheep anti-mouse IgG (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... the antigen was detected using chemiluminescence Western blotting detection reagents (Pierce ECL Western Blotting Substrate, Thermo Fisher Scientific Inc. ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by binding of antigen antibody complexes to protein A/G magnetic beads (Thermo scientific, Rockford, USA) for 1 h ...
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bioRxiv - Immunology 2020Quote: ... while non-Avi tagged antigens were biotinylated chemically using EZ-Link Sulfo-NHS-Biotin (Thermo Fisher, A39256). VH and VL sequences of SARS-CoV-2 control antibodies (COV2-2196 ...
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bioRxiv - Cancer Biology 2021Quote: ... epithelial membrane antigen (EMA; clone GP1.4, mouse monoclonal, dilution 1:1000, Thermo Fisher Scientific, Fremont, CA, USA), Sry-related HMG-BOX gene 10 (SOX10 ...
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bioRxiv - Cancer Biology 2020Quote: ... Antigen detection was accomplished with goat-ant-rabbit Cy3 secondary and chicken-anti-mouse Cy5 secondary (ThermoFisher, Waltham ...
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bioRxiv - Immunology 2020Quote: Antigen specific antibodies were depleted in a bead-based approach using Ni-NTA Magnetic beads (Thermo Scientific) as described (62) ...
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bioRxiv - Neuroscience 2022Quote: ... All sections were subjected to heat-mediated antigen retrieval in 10 mM sodium citrate (pH6.5; Fisher Scientific) in distilled water for 20 minutes at 80°C prior to washing 3 times in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibody/antigen complexes were recovered with Dynabeads M-280 sheep anti-rabbit IgG (Thermo Fisher Scientific, 11203D). Beads with DNA fragments were washed once with RIPA buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... Protein content of the antigen was determined with Coomassie (Bradford) Protein assay kit (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... and SV40 large T antigen (20 ng) suspended in Opti-MEM I Reduced-Serum Medium (ThermoFisher Scientific), mixed with 293fectin (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... Protein antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). OSP and LPS antigens were modified by 4-(4,6-dimethoxy[1,3,5]triazin-2-yl)-4-methyl-morpholinium and conjugated to Luminex Magplex carboxylated beads ...
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bioRxiv - Synthetic Biology 2023Quote: All RBD antigens were His-tagged and purified using HisPur Ni-NTA resin (Thermo Fisher Scientific, 88222). Cell supernatants were diluted with 1/3 volume of wash buffer (20 mM imidazole ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed by the use of Lab Vision PT Module (Thermo Fisher Scientific, Waltham, MA), in 10 mM citrate pH 6 ...
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bioRxiv - Cancer Biology 2024Quote: ... heat-induced (98°C) antigen retrieval was performed with a citrate buffer (pH 6.0) (Thermo Fisher Scientific), and slides were incubated with Triton X-100 0.25% in PBS for 5 minutes ...
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bioRxiv - Immunology 2020Quote: ... that does not express HLA DR and are Class II major histocompatibility (MHC) antigen negative was maintained in Iscove’s Modified Dulbecco’s Medium (IMDM, Invitrogen) supplemented with 20% FBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and any antigen–MS-Hu6 complex was captured by goat anti–human HRP–conjugated IgG (Invitrogen, Catalog # A18805).
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bioRxiv - Genetics 2020Quote: ... following de-paraffinization the slides were incubated in eBioscience IHC Antigen Retrieval Solution (ThermoFisher; Cat# 00-4955-58) and washed in 1x PBS three times for five minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The antibody-antigen complex was captured by incubating with lysis buffer prewashed proteinA/G beads (Thermo Fisher Scientific) for 2 h ...
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bioRxiv - Biochemistry 2020Quote: ... The antigen-antibody complexes were detected using the Pierce ECL chemi-luminescent detection system (Thermo Scientific, Rockford, IL) and visualized on the ChemiDoc XRS+ gel documentation system (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... The antigen/antibody complex was eluted from beads by boiling in Lane Marker Sample Buffer (#39001, Thermo Fisher) containing β-mercaptoethanol for SDS-PAGE and Western blotting.
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bioRxiv - Microbiology 2019Quote: ... SV40 T-antigens were detected using (pAb)108 and species-specific AlexaFluor 488 (Life Technologies, Thermo Fisher Scientific) antibodies ...
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bioRxiv - Microbiology 2020Quote: ... using antigens conjugated to NHS-activated agarose beads (Pierce™ NHS-Activated Agarose Spin Columns, Thermo Fisher Scientific). Antibodies were eluted in 0.1M glycine ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit or sheep antigens were raised in donkey and conjugated to Alexa 488 or Alexa 594 (Molecular Probes, ThermoFisher ...
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bioRxiv - Bioengineering 2021Quote: ... and subjected to heat- induced antigen retrieval using sodium citrate buffer(10 mM, pH 6, ThermoFisher, Waltham, MA) at >80°C ...
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bioRxiv - Microbiology 2022Quote: Purified F antigens were biotinylated using an EZ-link Sulfo-NHS -LC-Biotinylation kit (Thermo Fisher, cat#A39257) with a 1:1.3 molar ratio of biotin to F ...
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bioRxiv - Microbiology 2019Quote: ... SV40 T-antigens were detected using (pAb)108 and species-specific AlexaFluor 488 (Life Technologies, Thermo Fisher Scientific) antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... The antiserum was affinity-purified using the peptide antigen coupled to UltraLink™ Iodoacetyl gel (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2022Quote: ... after dewaxing and antigen retrieval non-specific antibody binding was minimised by incubating sections in CAS Block (Invitrogen). Primary antibodies were diluted in Dako antibody diluent (S0809 ...
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bioRxiv - Plant Biology 2022Quote: ... Chemiluminescence detection of antigen-antibody complexes was carried out with SuperSignal West Femto Western substrate (Thermo Fisher Scientific). As a loading control ...
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bioRxiv - Neuroscience 2023Quote: ... Antibody/antigen mixture was incubated with Dynabeads M-280 sheep anti-rabbit or mouse IgG (Thermo Fisher Scientific) for 6h at 4°C in the following day ...
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bioRxiv - Biophysics 2023Quote: ... 200 μL of the virus dilution containing 1µg-3µg p24 antigen was transferred to a poly-D-lysine coated glass coverslip (#1 chambered coverglass, Nunc™ Lab-Tek™ ...
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bioRxiv - Neuroscience 2023Quote: ... For antigen retrieval cells were incubated for ten minutes with 1 ug/ml proteinase K (Thermo Fisher Scientific) at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and coronavirus antigens were expressed in Expi293F cells by transient transfection in FreeStyle F17 expression media (Thermo Fisher) supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4 mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific ...