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Citations for Thermo Fisher :
301 - 317 of 317 citations for Borrelia Afzelii Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Total RNA was extracted from cell pellets collected in QuantiFERON-TB gold assay tubes without antigen using the Ribopure Ambion RNA isolation kit (Life Technologies, California, USA), and eluted RNA treated with DNase for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... and adjusted tissue and plasma extracts were added with the antiserum against OT (1:1,000 dilution) to each antigen-coated well of a 96-well microplate (F96 maxisorp nunc-immuno plate;Thermo Fisher Scientific, Roskilde, Denmark) and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... for antigen retrieval in a pressure cooker for 10 minutes then sections were blocked in CAS-block histochemical reagent (Thermo Fisher Scientific, 008120). Permeabilization was performed with 0.5% Triton X-100 in PBS for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... by measuring the expression of pluripotency surface antigen (SSEA4 1:20, Invitrogen, 12-8843-42, and TRA1-60 1:20, Invitrogen, 12-8863-82) by flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: ... Both FFPE and OCT cell array samples were incubated for 5min at 100°C in 1x Antigen Retrieval Solution (ThermoFisher, 00-4956-58) using a pressure cooker ...
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bioRxiv - Pathology 2023Quote: Slides were incubated at 90°C for 20 minutes in High pH IHC Antigen Retrieval Solution (Thermo Fisher Scientific Catalog Number 00-4956) and allowed to cool to room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... a female human embryonic kidney cell line transformed with the SV40 large T antigen was obtained from ATCC (#CRL-1573) and cultured in complete media consisting of DMEM (#11965092, GIBCO, Life Technologies; Carlsbad, CA) supplemented with 10% FBS (Seradigm Premium Grade HI FBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... rehydrated and underwent antigen unmasking by heat-induced epitope retrieval in 1× citrate buffer (pH 6.0; Thermo Scientific, Waltham, MA, USA #AP-9003-500). The sections were rinsed with PBS and blocked for 2 hours at room temperature using 10% normal donkey serum (NDS ...
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bioRxiv - Bioengineering 2020Quote: ... The specificity of the signals of antibodies against specific antigens was determined by performing a control experiment using compensation beads (Invitrogen, Cat# 01-1111-42).
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bioRxiv - Physiology 2020Quote: ... Antibodies to the antigen peptide were purified from the final bleed antiserum by affinity-purification using the AminoLink Plus Immobilization Kit (ThermoFisher Scientific, Waltham, MA, USA), with bound antibodies eluted using glycine elution buffer [6.3 ml of 100 mM glycine (VWR Chemicals ...
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bioRxiv - Immunology 2022Quote: ... Fluorescent antigen probes were used to detect VRC01gHL B cells with eOD-GT8 60-mer conjugated to Alexa Fluor 647 (Thermo Fisher Scientific, Catalog # A20173). Intracellular staining was performed with a fixation kit (BD Bioscience ...
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bioRxiv - Cell Biology 2023Quote: ... The solution was adjusted to 5 µg ml-1 in a total of 100 µl per well diluted antigen and were coated into Pierce™ Nickel Coated Plates (Thermo Fisher Scientific, #15142) at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... from singly sorted antigen-specific memory B cells were RT-PCR amplified using SuperScript III and AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Waltham, MA) under conditions previously described46.
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bioRxiv - Microbiology 2022Quote: Recombinant trimeric ectodomain of SARS-CoV-2 S protein with two proline mutations (rtS-ecto2P) as a vaccine antigen was produced using a Drosophila expression system (Thermo Fisher Scientific, Grand Island, NY). The protein sequence was modified to remove the furin cleavage site (RRAR to GSAG) ...
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bioRxiv - Immunology 2020Quote: ... then stained with surface and/or intracellular flow cytometry antibodies specific to antigens of interest using eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, Waltham, MA) according to manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: ... cells were stained following the ThermoFisher “BestProtocols: Staining Intracellular Antigens for Flow Cytometry – Protocol A” using the eBioscienceTM Permeabilization Buffer (ThermoFisher Scientific cat#: 00-8333-56) and eBioscienceTM IC Fixation Buffer (ThermoFisher Scientific cat# ...
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bioRxiv - Microbiology 2022Quote: ... Clones expressing different surface antigens were obtained by limiting dilution in 96-well culture plates placed in anaerobiosis chambers (Anaerogen® Compact, Thermo Scientific® Oxoid®, Cat. # AN0010C) at 37 °C during 5 days ...