Labshake search
Citations for Thermo Fisher :
1 - 50 of 317 citations for Borrelia Afzelii Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Borrelia burgdorferi genomic equivalents were calculated using an Applied Biosystems 7500 Real-Time PCR system (ThermoFisher Scientific) in conjunction with PowerUp SYBR Green Master Mix (ThermoFisher Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... then stained with a Borrelia polyclonal antibody diluted to 1:50 in 1x PBS (ThermoFisher, Waltham, MA, USA; FITC-PA1-73005) according to a previously established protocol [31] ...
-
bioRxiv - Microbiology 2022Quote: ... Individual samples were then screened using a duplex quantitative PCR for Borrelia burgdorferi and Borrelia miyamotoi in duplicate on an ABI 7500 Fast Real-Time PCR System using TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific).
-
bioRxiv - Neuroscience 2023Quote: ... Antigen retrieval was performed with Low pH IHC Antigen Retrieval Solution (Invitrogen) in a pressure cooker set to 100°C for 10 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval was performed using IHC Antigen Retrieval Solution (ThermoFisher, 00-4955-58) in a steamer for 40Dminutes ...
-
bioRxiv - Immunology 2022Quote: ... native control peptides and control antigens using a custom-designed antigen microarray (ThermoFisher Scientific, Immunodiagnostics)9 ...
-
bioRxiv - Physiology 2022Quote: ... Targeted antigen retrieval was performed by boiling sections in 1x IHC antigen retrieval solution (Invitrogen) for 15-20 mins ...
-
bioRxiv - Immunology 2023Quote: ... or control antigen PE (Thermo Fisher) were mixed with adjuvants per manufacturer’s protocols and added to cells immediately after plating ...
-
bioRxiv - Immunology 2023Quote: ... Monomeric antigens were biotinylated (ThermoFisher; A39257) at an optimized ratio which resulted in <1 biotin per molecule ...
-
bioRxiv - Immunology 2020Quote: ELISA of SARS-CoV-2 antigens was performed by coating antigens on MaxiSorp 96-well plates (ThermoFisher) at 2 µg/mL in 1X PBS [pH 7.4] overnight at 4 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval techniques were applied human tissue sections using IHC Antigen Retrieval Solution (00-4956-58, Invitrogen). These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes ...
-
bioRxiv - Cancer Biology 2024Quote: ... brain slices underwent an additional antigen retrieval step in 1X IHC Antigen Retrieval Solution (Invitrogen, 00-4956-58) for 15 minutes at 95°C prior to blocking.
-
bioRxiv - Cancer Biology 2023Quote: ... after which heat-induced antigen retrieval was performed in 1x low pH antigen retrieval solution (pH6, Thermo Fisher Scientific). Subsequently ...
-
bioRxiv - Neuroscience 2023Quote: ... A mild antigen retrieval step was introduced by immersing the slides into boiled antigen retrieval solution (00-4955-58, Invitrogen) for 10 min ...
-
bioRxiv - Immunology 2020Quote: ... antigens were directly coated to Nunc-absorb (ThermoFisher) plates overnight at 4°C or captured using streptavidin ...
-
bioRxiv - Immunology 2023Quote: ... Antigen printed pads were blocked with SuperBlock (ThermoFisher) for 1 hour before probed with serum samples (diluted at 1:100 in SuperBlock) ...
-
bioRxiv - Physiology 2024Quote: ... heated in antigen retrieval solution (Thermo Fisher Scientific) at 100 °C for 20 min ...
-
bioRxiv - Microbiology 2019Quote: ... 4 × 105 mouse splenocytes/well were plated with either with 5 μg/ml YFV-17D NS3 ATLTYRML peptide antigen (57) or with 50 μg/ml of total Vero E6 cellular antigen in RPMI 1640 medium (Gibco, Belgium) supplemented with 10% fetal bovine serum ...
-
bioRxiv - Immunology 2023Quote: NY-BR-1 antigen positive cell line CAMA-1 A2B2M and antigen negative SKMEL28 A2B2M were stained with CellTracker™ Deep Red Dye (Invitrogen, C34565) and seeded at 15000 and 10000 cells/well ...
-
bioRxiv - Neuroscience 2024Quote: ... slices underwent an additional antigen retrieval step (10 minutes in a pressure cooker in IHC Antigen Retrieval Solution (Invitrogen: 00-4956-58), followed by cooling in ice for 30 minutes ...
-
bioRxiv - Immunology 2021Quote: ... Cells were stained for surface antigens in PBS (Gibco) or Brilliant Stain Buffer (BD Biosciences ...
-
bioRxiv - Immunology 2021Quote: ... using 20 μg/mouse of antigen in Alum (Invitrogen)7,8 ...
-
bioRxiv - Immunology 2021Quote: ... Antigens were purified on C-tag column (Thermo Fisher) and quality-controlled by SDS-PAGE and for their level of endotoxin ...
-
bioRxiv - Immunology 2020Quote: ... Antigens were retrieved using 2.5% trypsin (Thermo scientific, 15090046) in 0.1% HEPES buffer ...
-
bioRxiv - Neuroscience 2023Quote: ... and antigen retrieved in citrate buffer pH 6.0 (ThermoFisher) in water bath at 80°C for 5 mins ...
-
bioRxiv - Developmental Biology 2023Quote: ... for antigen retrieval and 3% H2O2 (H325, Fisher Scientific) to inactivate endogenous peroxidase ...
-
bioRxiv - Cancer Biology 2021Quote: ... Paraffin sections of all tumors and normal skin were deparaffinized and antigen retrieval was performed using heat induced epitope retrieval method (HIER) with 1X Antigen Retrieval solution (Invitrogen #00-4955-58) in a pressure cooker at 95 for 10 min ...
-
bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 antigens were biotinylated using EDC (Thermo Fisher) and Sulfo-NHS-LCLC biotin (Thermo Fisher ...
-
bioRxiv - Immunology 2022Quote: ... Biotinylated antigens were coupled to FluoSphere NeutrAvidin beads (Thermo Fisher) and incubated with 10 μl 1:100 diluted plasma for 2h at 37°C to form immune-complexes ...
-
bioRxiv - Immunology 2022Quote: ... biotinylated antigens were coupled to FluoSphere NeutrAvidin beads (Thermo Fisher) and incubated with 10 μl 1:100 diluted plasma for 2h at 37°C to form immune complexes ...
-
bioRxiv - Immunology 2022Quote: ... biotinylated antigens were coupled to FluoSphere NeutrAvidin beads (Thermo Fisher) and to form immune-complexes incubated with 10 μl 1:10 diluted plasma samples for 2h at 37°C ...
-
bioRxiv - Cancer Biology 2020Quote: ... Citraconic anhydride for antigen retrieval was obtained from Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: ... Antigen/antibody complexes were visualized by Enhanced Chemiluminescence (Life Technologies) and signals were digitally acquired on a G:Box Chemi-XX6 imager with GeneSys software (Syngene) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Antigen/antibody complexes were visualized by Enhanced Chemiluminescence (Life Technologies) and signals were digitally acquired on a G:Box Chemi-XX6 imager with GeneSys software (Syngene) ...
-
bioRxiv - Cell Biology 2022Quote: ... followed by antigen retrieval in a 1x Citrate buffer (ThermoFisher) in a pressure cooker for one hour ...
-
bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigens were biotinylated using EDC (Thermo Fisher) and Sulfo-NHS-LCLC biotin (Thermo Fisher ...
-
bioRxiv - Bioengineering 2019Quote: ... antigens retrieved by treatmetn with 0.05 M EDTA (Ambion; AM9261) in a pressure cooker (Proteogenix ...
-
bioRxiv - Physiology 2019Quote: ... Tissue nonspecific antigens were blocked using 0.5% NGS (#16210064, Invitrogen) dissolved in PBS for 1h at RT and using constant agitation ...
-
bioRxiv - Cell Biology 2020Quote: ... or fluorescence-labeled secondary antibodies (for abundant antigens) (Thermo Fisher). For immunoblotting with HRP-conjugated secondary antibodies ...
-
Stromal transdifferentiation drives lymph node lipomatosis and induces extensive vascular remodelingbioRxiv - Pathology 2022Quote: ... Antigens were retrieved in 1mM ethylenediaminetetraacetic acid (EDTA) (pH9) (Invitrogen) at 97°C for 20min ...
-
bioRxiv - Systems Biology 2023Quote: ... underwent antigen retrieval and treated with Proteinase K (Ambion, 2546) at 1 ug/mL concentration ...
-
bioRxiv - Cancer Biology 2023Quote: ... Sections were used for antigen retrieval with trypsin digestion (Invitrogen) followed by blocking of nonspecific binding sites using normal goat serum (10% ...
-
bioRxiv - Immunology 2023Quote: ... to detect monomeric biotinylated antigens and anti-FITC antibody (Invitrogen) to detect Avidin-FITC conjugated tetrameric antigens ...
-
bioRxiv - Cell Biology 2023Quote: ... Antigen retrieval was performed using Citrate Buffer (pH:6.0, ThermoFisher) for paraffin embedded tissues ...
-
bioRxiv - Microbiology 2021Quote: ... Antigens were visualized using anti-species antibodies (Alexa Fluor, Thermo Scientific). Images were obtained using a 100× 1.4 NA objective 90 (Olympus ...
-
bioRxiv - Immunology 2021Quote: ... Antigens were then mixed with yellow-green fluorescent NeutrAvidin microspheres (ThermoFisher) at a ratio of 10 μl of beads per 10 μg of biotinylated protein and incubated at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: Staining of cell surface antigens was performed with antibodies from Invitrogen-ThermoFisher except for PE-Cy5.5-streptavidin conjugate ...
-
bioRxiv - Immunology 2021Quote: ... Antigen-specific IgG were isolated on NHS agarose resin (ThermoFisher; 26196) coupled to the protein of interest ...
-
bioRxiv - Immunology 2019Quote: ... Antigen-specific IgGs were isolated on NHS agarose resin (ThermoFisher; 26196) coupled to the protein of interest ...
-
bioRxiv - Cell Biology 2019Quote: ... Antigen-antibody complexes were visualized using chemilluminescence (Thermo Scientific, Pittsburgh, PA). Data was analyzed on an Azure C500 machine (Azure Biosystems ...