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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for 8 HYDROXY 1 3 DIOXOLO 4 5 G QUINOLINE 7 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Cultures were passaged every 5 to 7 days with collagenase type IV (Invitrogen; 1 mg/mL). The identities of all parental hESC and hiPSC lines were confirmed by DNA fingerprinting and all cell lines were regularly tested to exclude mycoplasma contaminations using a PCR-based assay ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 minute at 60°C)] on a QuantStudio 5 or QuantStudio 7 qPCR machine (Applied Biosystems). For qPCR assessments ...
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bioRxiv - Bioengineering 2021Quote: ... and Heparin scaffold conditioned media groups (n=5) across 21 days (Days 0, 3, 7, 14, 21) using a RNAqueous™-Micro Total RNA Isolation Kit (ThermoFisher Scientific). RNA was then reverse transcribed to cDNA using a QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: 10 uL of either Caspase-3 or −7 library glycerol stocks was used to inoculate 5 mL of auto-induction media (Invitrogen Magic Media) and allowed to incubate and express for 18 hours at 30 °C ...
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bioRxiv - Cell Biology 2023Quote: ... siCT (5’- CGUACGCGGAAUACUUCGAtt-3’, Ambion), siPALS1 (5’-UUCCUUAUGAUGAACUGGCtt-3’ ...
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bioRxiv - Cancer Biology 2023Quote: ... 3-5 μL RNAiMAX (Invitrogen) were added to 500 uL serum-free RPMI-1640 and incubated at room temperature for 5 min ...
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bioRxiv - Developmental Biology 2022Quote: ... All other lines were passaged at approximately 80% confluency (every 4-5 days on average) as tiny clusters (3-5 cells on average) using 0.5 mM EDTA (15575020, Gibco, concentration 10 mM).
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bioRxiv - Molecular Biology 2023Quote: ... Primary GR antibody (G-5, Santacruz sc-393232, 1:100) or MAP2 antibody (Invitrogen, 1:2000) incubations lasted for 1 h at RT followed by 3x washes and 1 h incubation in secondary antibody at RT (Cy3 Goat anti-mouse against GR ...
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bioRxiv - Immunology 2019Quote: ... the CellEvent Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) was added to the cells with complete media for 30 min in 37°C and the caspase-3/7 positive cells were measured on an Attune NXT flow cytometer (Life Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (Invitrogen) was used according to the manufacturer’s protocol with modest modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... caspase-3 and caspase-7 green apoptosis-assay reagent (Life Technologies) was added to the culture medium following manufacturer’s instructions ...
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bioRxiv - Zoology 2021Quote: ... and CellEvent Caspase-3/7 Green ReadyProbes Reagent (ThermoFisher Scientific, R37111) (CellEvent ...
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bioRxiv - Immunology 2024Quote: ... CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher, C10423) was added to cultures at 20 μM and incubated for 30 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... 3 μM pHrodo™ Red, succinimidyl ester (pHrodo™ Red, SE) (ThermoFisher Scientific; Cat. No. P36600) was added to the synaptosome fraction and incubated for 45 min at room temperature in the dark ...
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bioRxiv - Physiology 2021Quote: ... Crosslinking of Y-3 antibody and Protein A+G was done using sulfosuccinimidyl (4-iodoacetyl)aminobenzoate (Sulfo-SIAB, ThermoFisher 22327). For 1 mL of protein A+G agarose (ThermoFisher/Pierce #20421) ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were then centrifuged at 300 g for 3 min and fixed with 4 mM 3,3’– dithiobis(sulfosuccinimidyl propionate) (DTSSP, Life Technologies) in PBS at 37°C for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: ... were crossed for 2-3 days and then transferred to fly food made from 0.6 g of Carolina Formula 4-24 (Fisher Scientific) and 2 mL of ddH2O ...
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bioRxiv - Cell Biology 2023Quote: ... and the cell bodies were removed by centrifugation (1,150 g, 3 min, 4°C; Sorvall Legend XTR, Thermo Fisher Scientific). A sucrose cushion (10 ML of 25% sucrose in HMS ...
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bioRxiv - Molecular Biology 2024Quote: ... The enteroids were then pelleted at 300 x g for 3 min at 4 °C and washed once with advanced DMEM/F-12 basal media (Gibco). The enteroid pellet was resuspended in the desired volume of IntestiCult complete medium (Stemcell Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... BODIPY TR methyl ester (1:300, Life Technologies, Darmstadt, Germany) or Vybrant DiO (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... 1:250 for NHS ester-Alexa 405 (ThermoFisher Scientific #A7573) and 1:1000 for SytoxTM Deep red (ThermoFisher Scientific #S11380 ...
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bioRxiv - Bioengineering 2019Quote: ... The fixed cells were stained with 1 µM 2’-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5’-bi-1H-benzimidazole trihydrochloride trihydrate (Hoechst 33342, Invitrogen) for 5 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... An internal standard was prepared from the isotope-labeled standards in 3% acetonitrile with 0.1% formic acid with 5 fmol/µL of Peptide Retention Time Calibration (PRTC) mixture (ThermoFisher Scientific) and 10 µg/mL E ...
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bioRxiv - Plant Biology 2022Quote: ... and Syto 13 Green Fluorescent Nucleic Acid Stain (5 μM mL-1, ThermoFisher Scientific) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Leukocytes were washed with cold PBS followed by another centrifugation step (300 x g 5 minutes) and resuspended in 5 mL of 5 μg mL-1 of Hoechst 33342 (Thermo Fisher Scientific) diluted in warm PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescence-labelled 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE) was obtained from Molecular Probes (Eugene, Oregon, US). Sucrose was purchased from XiLong Chemical Co. ...
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bioRxiv - Microbiology 2019Quote: ... and 5 g/L Albumax I (Invitrogen). Unless otherwise indicated ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5 g/L ammonium sulfate (Fisher Scientific), 20 g/L dextrose ...
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bioRxiv - Genetics 2020Quote: ... 5 g/L ammonium sulfate (Fisher Scientific), 20 g/L dextrose (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... 5 g/L Albumax II (Life Technologies) and 25 μg/mL gentamicin ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 g/L Albumax II (Life Technologies) and 25 µg/mL gentamicin ...
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bioRxiv - Microbiology 2021Quote: ... and 40 µg/mL X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside; Thermo Scientific). Plates were incubated at room temperature for 48-72 h ...
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bioRxiv - Bioengineering 2020Quote: Before staining with either 5-bromo-4-chloro-3’-indolyphosphate and nitro-blue tetrazolium (BCIP/NBT, ThermoFisher) or Alizarin Red S (ARS ...
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bioRxiv - Microbiology 2024Quote: ... containing 100 mg/ml of X-gal (5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Thermofisher) to confirm CFU/ml counts.
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bioRxiv - Molecular Biology 2023Quote: ... 10μg of total protein were heat-denatured for 5 minutes at 100°C before loading onto NuPAGE 3– 8% TRIS Acetate Midi Gel (Novex, Life Technologies) and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Biophysics 2023Quote: ... against PIEZO1 that targets the 5’-AGCATTGAAGCGTAACAGGG-PAM-3’ at Chr.8: 122513787 - 122513809 on GRCm38 was purchased from Invitrogen (A35533). The cells were transfected with the Cas9 enzyme (TrueCut Cas9 Protein v2 ...
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bioRxiv - Plant Biology 2023Quote: ... 4 or 8 μM of zeocin (Thermo Fisher Scientific) and grown for further 8 days in LD before fresh weight measurement ...
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bioRxiv - Immunology 2023Quote: ... a liver burn injury of approximately 1 mm3 was made on which a droplet of pHrodo Red succinimidyl ester (4 μM; Thermo Fisher Scientific) was administered ...
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bioRxiv - Developmental Biology 2020Quote: ... sections were rinsed in 3% acetic acid (Fisher Scientific) for 3 minutes and incubated at room temperature for 30 minutes in 1% Alcian Blue 8GX (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... and 3-mercaptopropionic acid were purchased from ACROS ORGANICS. Hydrogen tetrachloroaurate(III ...
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bioRxiv - Developmental Biology 2023Quote: ... acidified to pH2-3 by trifluoroacetic acid (Thermofisher, 85183), and desalted with a Pierce C18 spin column (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... were washed twice with 300 μL 1X TBS-T (2.4 g Tris-Base, 8 g NaCl, 0.1 % Tween20) using a magnetic stand (ThermoFisher) before coupling them with 200 μl of B domains in TBS at 0.125 ng/ml ...
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bioRxiv - Genomics 2021Quote: The metabolism of 4-hydroxyphenylacetic acid (p-hydroxyphenylacetic acid; Acros Organics, Product code: 121710250) and gentisic acid (2,5-dihydroxybenzoic acid ...
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bioRxiv - Cancer Biology 2021Quote: ... Culture medium was refreshed every 2–3 days and organoids were passaged 1:2–1:6 every 7–21 days using TrypLE Express (Thermo Fisher). For co-culturing ...
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Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: ... A pre-warmed buffer containing DPBS supplemented with 10% FBS and CellEvent Caspase 3/7 Green (1 μL/1 mL of buffer; ThermoFisher C10427) were added to the cells ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were incubated for 30 min in standard media with CellEvent Caspase 3/7 (1:400, C10423, Invitrogen) prior to imaging to observe apoptosis.
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bioRxiv - Developmental Biology 2023Quote: ... 20% Knockout Serum Replacement, 1% Glutamax, 1% Non-Essential Amino Acids, 0.1% ß-Mercaptoethanol, and 8 ng/mL bFGF, Thermo Fisher Scientific), and passaged weekly by manual disruption of 6-10 representative colonies ...
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bioRxiv - Immunology 2023Quote: ... and cellular debris spun down at 12,000 g for 5 minutes before being subjected to electrophoresis on Bolt 4- 12% Bis-Tris gels from Invitrogen/Thermo Fisher (Cat ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were subsequently centrifuged for 5 minutes at 200 x g and resuspended in 4% paraformaldehyde (Thermo Scientific Chemicals) for 30 minutes at 22°C ...
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bioRxiv - Neuroscience 2021Quote: ... 7 dpf larvae were exposed to 5 mM BAPTA (Invitrogen) in E3 for 10 minutes ...