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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for 8 Bromo 3 iodo quinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... CaVβ3-stable cells were grown in suspension at 37°C supplied with 8% CO2 in FreeStyle 293 Expression Medium (Gibco) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen) and transferred to 0.2 µm PVDF membranes via wet tank or semi-dry method (specified in the supplementary information) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were resolved on NuPAGE 3-8% (wt/vol) Tris-acetate or 4-12% (wt/vol) Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2023Quote: ... 10-15ug total protein was loaded into wells of 3-8% Tris-Acetate gels and separated in tris acetate buffer (Invitrogen) ran at 65v for 30 minutes and then 125v until the dye front reached the bottom of the gel ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... were incubated at 37°C for 2 h and analyzed by SDS-PAGE on a 3-8% tris-acete NuPAGE gel (Invitrogen) and stained with Pierce Silver Stain Kit (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Perfusion was subsequently changed to bicarbonate-buffered extracellular solution supplemented with the voltage- sensitive dye Di-8-ANEPPS at 3 µM (D3167, Invitrogen). Following dye loading for 4 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Samples were drawn after 0.5, 1, 3, 5, 8, and 24 h incubation and centrifuged (21500 xg, 4 °C, 20 min) (Thermo Scientific SL 8R Centrifuge ...
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bioRxiv - Microbiology 2023Quote: ... then 5 μg total protein in this buffer was separated by SDS-PAGE on a NuPAGE 3-8% Tris-Acetate Midi Gel (Invitrogen). Protein was transferred to a 0.45 μm PVDF Membrane (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: ... and the lysate was subjected to Western blot on NuPAGE Novex 3–8% Tris-acetate gradient gels (Invitrogen™ # EA0375BOX), transferred to a nitrocellulose membrane and blotted against OPA1 (Anti-Mouse ...
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bioRxiv - Developmental Biology 2024Quote: Monolayers and EBs from day 3 to day 8 were dissociated to single cells with TypLE 1X (Thermo Fisher, 12563029) for 5 minutes at 37°C ...
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bioRxiv - Immunology 2020Quote: ... pH 8 (Ambion) for qPCR ...
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bioRxiv - Neuroscience 2022Quote: ... 8% FBS (GIBCO), 100 mM Taurine (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2023Quote: ... pH 8 (Invitrogen, 1 M Tris pH 8.0 ...
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bioRxiv - Systems Biology 2023Quote: ... pH 8 (Invitrogen, 1 M Tris pH 8.0 ...
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bioRxiv - Systems Biology 2023Quote: ... pH 8 (Invitrogen, 1 M Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... pH=8 (ThermoFisher, MA ...
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bioRxiv - Developmental Biology 2023Quote: Essential 8 medium consisted of essential 8 basal medium (Gibco, A1517001) supplemented with 50x essential 8 supplement.
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bioRxiv - Bioengineering 2023Quote: ... at pH 8 containing 8 M urea (Thermo Fisher Scientific, USA). To reduce the sample ...
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bioRxiv - Immunology 2021Quote: ... was added to a concentration of 1X and 12.5-30 μg of total protein from each sample was resolved on NuPAGETM 4-12% BisTris (Phospho-PMK-1 and Total-PMK-1) or NuPAGETM 3-8% TrisAcetate (TIR-1::3xFLAG) gels (ThermoFisher Scientific), transferred to nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples of SMG6-3xFLAG in vitro translation reactions were run on 3-8% Tris-Acetate NuPAGE gel (Invitrogen, Cat. No EA03752BOX). Transfer onto nitrocellulose membranes was performed using the iBlot 2 Dry Blotting System (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10-20 µg of protein loaded on a 3%–8% NuPAGE Tris-Acetate Gel in tris-acetate buffer (Novex Life Technologies) or a 4-12% NuPAGE TrisBis NuPAGE gels in MES buffer (Novex Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... and checked for purity and quality on a silver-stained SDS-PAGE gel (3-8% Tris-Acetate Novex, Thermo Fisher Scientific). The anti-NTD neutralizing antibody NTD-18 and the pan-coronavirus anti-S2 non-neutralizing antibody Ab-10 were previously described 9,10.
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bioRxiv - Cancer Biology 2019Quote: ... LDHA proteins in tumor extract were separated on 4% to 20% Tris-Glycine gel and CD31 was separated on NuPAGE 3 to 8 % Tris-Acetate gel (Life Technologies) by SDS-PAGE and were transferred to nitrocellulose membrane ...
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bioRxiv - Genomics 2020Quote: ... 1% Triton X-100 and 10% glycerol and resolved on 3–8% pre-cast gradient Tris-Acetate gels (Invitrogen, Waltham, MA). For mouse samples ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μg protein/lane was then separated under denaturing conditions on reducing 3~8% Tris-Acetate gels (NuPAGE, Thermo Fisher Scientific), and HiMark protein standard (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were denatured by adding 4xLDS buffer and heating at 70°C for 10 min before being separated in 3-8% Tris-acetate gels (Thermo Fisher) run at 150 V for 3 hours ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were then resolved on 4-12% Bis-Tris gels (for lower molecular weight proteins) or 3-8% Tris-Acetate gels (for higher molecular weight proteins) (NuPage, Thermo-Fisher Scientific) and run in MOPS or Tris-Acetate running buffer respectively ...
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bioRxiv - Cell Biology 2021Quote: ... were transiently transfected into 8 × 104 U2OS 2-6-3 cells in 4-well chamber slides using Lipofectamine 2000 reagent (Invitrogen, 11668019) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... were loaded on to NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate 1.0 mm Mini Protein Gels (Invitrogen, NP0321, EA0375PK2) buffer alongside PageRuler Protein Ladder (5 µL/lane ...
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bioRxiv - Molecular Biology 2022Quote: Protein extracts were resolved using either home-made SDS-PAGE gels or 3-8% NuPAGE Tris-Acetate gels (Thermo Fisher Scientific), when analysing proteins of a molecular weight >180 kDa ...
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bioRxiv - Pathology 2022Quote: ... Protein concentration was determined with Bio-Rad Protein Assay Kit II and 15 μg sample protein was loaded in a NuPAGE 3-8 % Tris-Acetate gel (Thermo Fisher). The gel was run at a constant voltage of 150 V for 4 h ...
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bioRxiv - Biophysics 2022Quote: ... Purified surface proteins or total cell lysates were separated on a NuPage 3-8 % Tris-acetate protein gel (Thermo Fisher Scientific) at 200 V for 40 min and transferred to a PVDF membrane ...
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bioRxiv - Immunology 2022Quote: ... The quality/purity of the different purified fractions was evaluated by SDS-PAGE using 3–8% Tris– Acetate gels (Life Technologies) under non-reducing conditions followed by silver staining (Silver Stain kit ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated at 95°C for 5 min and the supernatant loaded onto a either 3-8% Tris-acetate or 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Genetics 2019Quote: ... 40-50μg of protein was analysed by SDS–polyacrylamide gel electrophoresis (SDS–PAGE) using 3-8% NuPAGE Tris-Acetate protein gels (Life Technologies) and transferred onto nitrocellulose membrane using Xcell Sure Lock Mini Cell electrophoresis tanks (Novex ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were passed through a 25G syringe five times to reduce viscosity and separated on a 3%-8% NuPAGE Tris-Acetate gel (Thermo Fisher). Proteins were transferred onto a polyvinylidene fluoride membrane (Thermo Scientific ...
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bioRxiv - Genomics 2020Quote: ... An estimated 60 µg of protein was loaded per well on a NuPAGE Novex 3-8 % Tris-Acetate Protein gel (Life Technologies), separated by electrophoresis and blotted on polyvinylidene fluoride membranes ...
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bioRxiv - Cancer Biology 2021Quote: ... for 10 min at 70°C and separated on 4-12% Bis/Tris-gels or 3-8% Tris/Acetat-Gels (Thermo Fisher). After separation ...
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bioRxiv - Developmental Biology 2021Quote: ... FAPs were plated at a starting density of 3 X 104 cells/cm2 and grown for 8 days in DMEM (Life Technologies) containing 5% FBS plus 1% Pen/Strep ...
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bioRxiv - Developmental Biology 2020Quote: ... For the detection of Gli3 10μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
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bioRxiv - Microbiology 2022Quote: Proteins (8 μM final) were incubated with 0.16 mM of (2'-(or-3')-O-(trinitrophenyl) adenosine triphosphate (TNP-ATP) (Thermo Fisher Scientific), in 50 μl of Binding Buffer (10 mM HEPES-KOH (pH 8) ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Molecular Biology 2022Quote: ... 2-3 mg/mL proteins were applied to glow-discharged M026-Au300-R20/20 Copper grid (CryoMatrix) and blotted for 3 s at 8 ℃ with 100% humidity and plunge-frozen in VitroBot Mark IV (Thermo Fisher).
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bioRxiv - Neuroscience 2023Quote: ... To revive hiPSCs from cryopreservation cells were thawed by incubation for 2 minutes at 37°C and retrieved by centrifugation (200 x g for 3 minutes) prior to resuspension in Essential 8 (E8, (A1517001, Life Technologies) medium with 1’RevitaCell ...
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bioRxiv - Molecular Biology 2023Quote: ... 10μg of total protein were heat-denatured for 5 minutes at 100°C before loading onto NuPAGE 3– 8% TRIS Acetate Midi Gel (Novex, Life Technologies) and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Genomics 2023Quote: ... Immunoblotting was performed according to standard procedures with the following modifications: a 3-8% tris acetate gel (Thermo Fisher Scientific, EA03752) was used and samples were run for 2 hours 15min at 80V at 4 °C in 1X tris-acetate running buffer (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... against PIEZO1 that targets the 5’-AGCATTGAAGCGTAACAGGG-PAM-3’ at Chr.8: 122513787 - 122513809 on GRCm38 was purchased from Invitrogen (A35533). The cells were transfected with the Cas9 enzyme (TrueCut Cas9 Protein v2 ...
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bioRxiv - Biophysics 2023Quote: ... the media was removed and the cells were incubated with 8 µM CellEventTM Caspase 3-7 green detection reagent (Thermo Fisher) in PBS containing 5% FBS for 30 min at 37 °C ...
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bioRxiv - Genetics 2023Quote: ... the denatured nuclear extract was subjected to electrophoresis on a precast 3-8% Tris-Acetate polyacrylamide gel (Thermo Fisher Scientific, EA0378BOX) in 1× Tris-Acetate SDS running buffer (Thermo Fisher Scientific ...