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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for 7 bromoquinolin 2 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... Library concentration was normalized to 2 nM and concentrations validated by qPCR on a ViiA-7 real-time thermocycler (Applied Biosystems), using qPCR primers recommended in Illumina’s qPCR protocol and Illumina’s PhiX control library used as a standard ...
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bioRxiv - Immunology 2023Quote: ... Serum starved PANC-1 and MiaPaCa-2 cells were incubated with human properdin (20 µg/ml) and CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) (5 μM ...
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bioRxiv - Microbiology 2023Quote: For detection of NO in treated mycobacteria DAF-FM diacetate (4-Amino-5-Methylamino-2’,7’-Difluorofluorescein),27 purchased from Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated with fresh DMEM containing DDAO galactoside (9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) β-D-Galactopyranoside) (Molecular Probes™) for 2 hours ...
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bioRxiv - Plant Biology 2024Quote: ... Ten-μg protein of each sample were separated by electrophoresis in 15% SDS-polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (Roth) for 7 min at 20V using the iBlot 2 Dry Blotting System (ThermoFisher Scientific), before blocking in TBS-T with 5% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... and TNFα (10 ng/ml) for 6 h and stained for cleaved Caspase 3/7 green (5 µM) and propidium iodide (2 µM) (Thermo Scientific) for an additional 30 min ...
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bioRxiv - Plant Biology 2024Quote: ... fluorescence was observed under a confocal microscope (LSM880NLO, Zeiss, Germany) using 2’,7’-dichlorodihydro fluorescein diacetate (H2DCFDA, 10 InvM, Invitrogen, USA) as described by Yu et al ...
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bioRxiv - Microbiology 2024Quote: ... 7 μl per injection was loaded onto a 2 mm by 0.3 mm Acclaim PepMap C18 trap column (Thermo Fisher Scientific) in 0.1% TFA at 15 μl/min before the trap being switched to elute at 0.25 μl/min through a 50 cm by 75 μm EASY-Spray C18 column ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cell Biology 2024Quote: ... The flow-through was then dried in a speed-vac and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cell Biology 2019Quote: ... or caspases 3/7 (Caspase-3/7 Green ReadyProbes™ reagent with a DEVD sequence, Molecular Probes).
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bioRxiv - Cell Biology 2019Quote: ... or caspases 3/7 (Caspase-3/7 Green ReadyProbes™ reagent with a DEVD sequence, Molecular Probes).
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bioRxiv - Immunology 2023Quote: ... The caspase-3/7 activity assay contained CellEvent Caspase- 3/7 Green Detection Reagent (Thermo Fisher, C10723) at a final ...
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bioRxiv - Neuroscience 2019Quote: Human embryonic kidney (HEK)-293T cells in log-phase growth were rapidly transfected by using the standard Lipofect Amine reagent (Invitrogen), with GFP-Vector plus helper plasmids to develop virions (Cenciarelli et al. ...
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bioRxiv - Biophysics 2021Quote: ... Oregon Green-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (OG-DHPE) and Texas Red-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanol-amine (TR-DHPE) was obtained from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2021Quote: Fluorescent polystyrene beads 0.2 μm in size (FluoSpheres sulfate microspheres F8848, carboxylate-modified microspheres F8811, amine-modified microsphere F8764; Thermo Fisher) were diluted 300 times to 0.02% (wt/vol ...
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bioRxiv - Bioengineering 2022Quote: ... 100 μL of amine bearing microbeads solution was firstly reacted with 10 mM bis(sulfosuccinimidyl)suberate crosslinker (BS3; Life Technologies) in phosphate buffered saline (PBS ...
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bioRxiv - Biophysics 2022Quote: Fluorescent labeling was performed by targeting histone amine groups of pre-assembled nucleosomes using Alexa Fluor 647 succinimidyl ester (ThermoFisher), following the previously reported strategy20 ...
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bioRxiv - Neuroscience 2020Quote: ... Each probe is 18-22nt long with a 3’ end amine-modified nucleotide that allows directly couple to an NHS-ester dye according to the manufacturer’s instructions (Life Technologies). Dye-labeled probes were separated from the excess free dyes using the Qiagen Nucleotide Removal Columns ...
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bioRxiv - Biophysics 2020Quote: Wild-type and the three LXXAA mutants of TIF2NRID were amine-labelled with a fluorescent probe (Alexa 488 NHS Ester, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The NS probe was purchased with a terminal amine group and manually conjugated to the Alexa Fluor 488 fluorophore using the Alexa Fluor 488 Oligonucleotide Amine Labeling Kit (Invitrogen). The NA and PB2 probes were labeled by the manufacturer with the Quasar 570 and Quasar 670 fluorophores ...
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bioRxiv - Neuroscience 2021Quote: ... the basal nucleus was pressure-injected at various dorsoventral and rostrocaudal sites with either 40 nl of the bidirectional tracers Lucifer yellow conjugated to dextran amine (LY; Invitrogen), Fluorescein conjugated to dextran amine (FS ...
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bioRxiv - Biochemistry 2021Quote: ... we used the same amount of chromatin proteins for different conditions and labeled them with different amine-reactive TMT 6-plex reagents (ThermoFisher). Then we mixed these samples together and carried out mass spectrometry analysis ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed with FACs buffer by centrifugation before staining with amine-reactive Live/Dead Fixable violet viability cell stain as per the manufacturer’s instructions (Life Technologies). Cells were then incubated for 30 minutes at room temperature with optimal dilutions of the following antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... 2mg/ml of proteins was used and conjugation was done with amine-reactive (NHS-ester) fluorophores as per the instructions given by Invitrogen. The unlabeled fluorophores were separated using PD-10 columns (GE Healthcare).
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bioRxiv - Synthetic Biology 2022Quote: ... Conjugation of the dye to the polymer was achieved by adding 5 mg of the amine-reactive Pacific Blue succinimidyl ester (ThermoFisher) to a solution of 20 mg AD (Fina Biosolutions ...
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bioRxiv - Neuroscience 2023Quote: ... at varying depths was made targeting the deep layers of the primary motor cortex using 10% biotinylated dextran amine (BDA; 10,000 MW) (Life Technologies) (n=4 ...
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bioRxiv - Neuroscience 2023Quote: ... brains were washed trice with PBS for 5 min and free amines were anchored with 0.1mg/mL succinimidyl ester of 6-((Acryloyl)amino)hexanoic acid (Acryloyl-X, SE, Life Technologies) in PBS at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: JCPyV-infected and mock-infected astrocytes were detached using TrypLE (GibcoTM) and labeled with aqua blue amine dye (Life Technologies) for 25 min at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... LIG1 proteins were then fluorescently labeled using amine reactive NHS ester conjugated Alexa Fluor (AF)488 according to the manufacturer’s instructions (Thermo Fisher). The protein concentrations and labeling efficiency were finally determined by calculating the molar ratio of dye to protein within the solution by measuring absorbance at 280 nm and 488 nm.
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bioRxiv - Immunology 2021Quote: ... 1:100 human IgG (1 mg/ml) as FcR block and 2 % FCS using the following staining reagents: 7-AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Microbiology 2021Quote: ... ATPase activity was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside–purine nucleoside phosphorylase reaction to detect released inorganic phosphate (EnzChek kit; Thermo Fisher Scientific) (62) ...
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bioRxiv - Molecular Biology 2022Quote: ... HCA-7 and MDCK cells were allowed to differentiate for 7 days after plating at 100% confluency in the presence of 2 µg/mL doxycycline (Fisher Scientific AAJ6042203) when inducing transgene and/or reporter transcript expression ...
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bioRxiv - Microbiology 2021Quote: PBMCs were plated into a 10 cm dish at 7×107 cells/dish and adhered for 2 hours in AIM-V (Gibco-BRL, CA). The nonadherent cells were removed gently and frozen as the source of lymphocytes for co-culture use ...
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bioRxiv - Immunology 2022Quote: The production of NO was evaluated using 4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate (DAF-FM DA)(D23844; Invitrogen, Waltham, MA). Following leukocyte isolation ...
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bioRxiv - Systems Biology 2019Quote: Determination of intracellular levels of total ROS was carried out by flow cytometry using 2’,7’-dichlorodihydrofluorescein diacetate (DCH2FDA, Thermo Fisher Scientific), as previously described (Amara et al ...
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bioRxiv - Microbiology 2020Quote: Amount of ROS in dissected guts (from 10 animals) was estimated using 2’,7’-Dichlorodihydrofluorescein diacetate (H2DCFDA, C6827, ThermoFisher Scientific, MA, USA), following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with mouse mAb SARS-CoV-2 nucleocapsid antibody (SinoBiological, 1:100) and rabbit Claudin 7 polyclonal antibody (ThermoFisher, 1:200) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Staining was performed on ice for 25 minutes in PBS with 2 % FCS using the following antibodies: 7- AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Bioengineering 2022Quote: ... Cultures were washed three times with washing solution and then incubated for 30 min at room temperature on the OrganoFlow (7°, 2-min intervals) with Alexa Fluor 488 (#a32731; Invitrogen; 1:250) and Alexa Fluor 647 (#a31571 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed three times with unsupplemented neurobasal media and loaded with 200μM 2’,7’-dichlorofluorescein diacetate (DCFH-DA) fluorescent dye (Thermo Fisher Scientific, MA) for 30 minutes at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... Mock-treated and infected roots of WT and HvMPK3 KO plants were incubated in 30 μM 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA; Cat no. C6827, Invitrogen™, USA) and PI (1 mg.ml-1 ...
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bioRxiv - Neuroscience 2023Quote: Larval zebrafish at 4–7 days post-fertilization were anesthetized (0.025% MS-222, Syndel) and mounted in 2% low melting point agarose (Thermo Fisher Scientific 16520), dorsal-up ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were washed and re-suspended in PBS buffer free of KCl and incubated in the dark with 25-μM of 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) suspended in dimethylsulfoxide (DMSO) for 60 min at room temperature (Molecular Probes, Invitrogen). After incubation ...
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bioRxiv - Bioengineering 2022Quote: The level of reactive oxygen species was assessed in the homogenate supernatant of RPE and retina tissue with using 2′–7′-dichlorofluorescein diacetate (DCFH-DA; ThermoFisher Scientific Inc.) molecular probes ...
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bioRxiv - Molecular Biology 2023Quote: ... The iPSCs were then cultured for 7 days in Neurobasal/DMEM-F12 medium (1:1 v/v) containing 2% B27 (Gibco, 17054–044), 1% N2 (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cell Biology 2024Quote: ... The flow-through was then dried in a speed-vac and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to nitrocellulose membrane at 20 V for 7 minutes using the iBlot™ 2 Transfer Stack (Thermo Fisher Scientific). Membranes were blocked in 5% non-fat milk powder in TBS+0.1% Tween (TBST ...