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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for 7 bromoquinolin 2 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Total RNAs from 7-d-old seedlings grown on 1/2 MS with or without 1 μM ABA were extracted using TRIzol (Invitrogen, Carlsbad, CA, USA) or Universal Plant Total RNA Extraction Kits (Spin-column)-I (BioTeke ...
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bioRxiv - Cancer Biology 2021Quote: ... After 3 and 7 days of treatment cells were stained with 2% crystal violet (CV) (40583100, Acros Organics, Fisher Scientific, Thermo Fisher Scientific) and dried ...
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bioRxiv - Cancer Biology 2021Quote: ... After 3 and 7 days of treatment cells were stained with 2% crystal violet (CV) (40583100, Acros Organics, Fisher Scientific, Thermo Fisher Scientific) and dried ...
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bioRxiv - Molecular Biology 2022Quote: ... 4ºC) and incubated with 50 μL of 5 μM DAF-FM (4-amino-5methylamino-2’,7’-dichlorofluorescein diacetate, Life Technologies, Eugene, OR, USA) diluted in 1X PBS for 30 min at 34 °C ...
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bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescence-labelled 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE) was obtained from Molecular Probes (Eugene, Oregon, US). Sucrose was purchased from XiLong Chemical Co. ...
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bioRxiv - Biophysics 2020Quote: ... Subsequently, protein samples were washed once using Zeba™ Spin desalting columns (7 kDa MWCO, 2 mL) (Thermo Fisher Scientific, Waltham, MA, USA) equilibrated with degassed buffer A ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were washed then incubated in 8 μM of the ROS sensitive probe 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester (CM-H2DCFDA) (Molecular Probes, Eugene Oregon), for 45 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... After a clarifying spin for 15 min at 14,000 ×g and 4°C 2 μl (MICOS complex) or 7 μl (all other mitochondrial protein complexes) G-250 Sample Additive (Invitrogen, Waltham, Massachusetts, USA) containing 40% glycerol were added ...
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bioRxiv - Bioengineering 2023Quote: ... For each sample 2 μg of solubilized silk protein was loaded into a NuPAGE™ 7% Tris-Acetate gel (EA03585BOX, Invitrogen, Waltham, MA) under reducing conditions ...
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bioRxiv - Biochemistry 2023Quote: ... and transferred onto a 0.45 μm nitrocellulose membrane for 7 min at 20 V with the i-Blot 2 (Thermo Fisher, Waltham, MA, USA). Blocking ...
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bioRxiv - Biophysics 2021Quote: ... Unreacted amine groups on the silane molecules were capped using NHS-sulfo acetate (10 mg/ml for 30 mins; Thermo Fisher). PEG-functionalized AFM cantilevers were incubated in 1 mg/ml Bovine Serum Albumin 55 overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Covalent labeling of amine groups of the proteins with Alexa Fluor 488 or Alexa Fluor 647 succinimidyl ester dyes (Molecular probes/Invitrogen) was conducted in its polymer-assembled form as previously described (González et al ...
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bioRxiv - Immunology 2022Quote: ... The 3’-end of each probe was modified with an amine group and coupled to either tetramethylrhodamine (TMR; Thermo Fisher Scientific), Texas Red-X (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: TE proteins were fluorescently labeled with the amine-reactive dye Alexa Fluor 647 NHS-Ester (Thermo Fisher Scientific, Rockford, IL, USA) as follows ...
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bioRxiv - Biochemistry 2019Quote: ... peptide concentration and primary amine reactivity were quantified using the Pierce quantitative fluorometric peptide assay (Thermo Fisher Scientific; cat. no. 23290) and Pierce quantitative colorimetric peptide assay (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed with FACS buffer consisting of PBS + 1% FCS and incubated for 30 minutes at room temperature with optimal dilutions of the amine-reactive Live/Dead Fixable Red viability cell stain (Life Technologies) and the antibodies CD4 PerCP-Cy5.5 ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μL the corresponding amine-based tandem mass tag (TMT) 10-plex reagents (10 μg/μL) (Thermo Fisher Scientific, Cat # 90406) was added and incubated for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Cross-linking occured for 30 min in a rolling tube after which beads were washed with 0.2 M ethanol amine pH 8 (#149582500, Thermo Fisher Scientific) to quench the crosslinking reaction.
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bioRxiv - Microbiology 2022Quote: SpRecAA488 was made by covalently modifying primary amines (lysines or N-ter) of the protein with Alexa 488-succinidimyl ester (Molecular Probes, ThermoFisher), in presence of an excess of ssDNA (M13 mp18 ...
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bioRxiv - Neuroscience 2023Quote: ... injections into the spinal cord rostral to the lesion as a retrograde tracer or injections of biotinylated dextran amines (BDA, D1956; 10,000 kD, 10.0% w/v, ThermoFisher Scientific, Waltham, MA) into the motor cortex as an anterograde tracer to visualize the CST ...
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bioRxiv - Neuroscience 2023Quote: ... then reverse transcribed using random primers with a N-terminal amine group and Revert-aid H-minus reverse transcriptase (Thermo Fisher). On the second day ...
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bioRxiv - Biochemistry 2024Quote: Fluorescent labelling of Tau and 14-3-3ζ protein for microscopy assays was done using amine-reactive DyLight405 or DyLight488-NHS ester (Thermo Scientific) following the manufacturer instructions and the established protocol 34.
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bioRxiv - Cell Biology 2023Quote: ... The carboxyl groups on the bead surfaces were functionalized with amine-reactive groups via N- ethyl-N’-(3-(dimethylamino)propyl)carbodiimide (EDC) and sulfo-N-hydroxysuccinimide (sulfo-NHS, Thermo Fisher) crosslinking for 20 minutes at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... were used at 50-100 nM to transfect HUVEC (P3-P6) that were seeded at 50% confluency onto gelatin (1 mg/ml) coated flasks with siPORT Amine (Ambion, MA) per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The LC-MS data analysis of the amine- and the glycolysis- and TCA-compounds was performed using the TraceFinder software (Version 5.1, Thermo Fisher Scientific). The identity of each compound was validated by authentic reference compounds ...
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bioRxiv - Biochemistry 2024Quote: The synthesized glycated amines were acquired in parallel reaction monitoring (PRM) using a UHPLC-High-Resolution Accurate mass spectrometer (LC-HRAM, Orbitrap, ThermoFisher Scientific) equipped with a heated electrospray ionization (HESI ...
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bioRxiv - Immunology 2020Quote: ... Detection of active caspase 3/7 was accomplished using CellEvent™ Caspase-3/7 Red Detection Reagent (ThermoFisher Scientific). Sorted CD4SP Rag1GFP+ thymocytes were stained with CD5-PerCP-Cy5.5 (53-7.3 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were cοtransfected with 7 µg CRISPR plasmid and 7 µg non-linearized HDR template using Lipofectamine 3000 according to the instructions (ThermoFisher). 48 hours after transfection ...
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bioRxiv - Microbiology 2022Quote: ... and the vesicular stomatitis virus G (VSV-G)-expressing plasmid at a ratio of 7:7:1 (5 μg total amount of DNA) using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 5 mM EDTA and 1% BSA in HBSS) and 2μL of 7-aminoactinomycin D (7-AAD, Thermo Fisher Scientific, A1310) at a concentration of 0.1 mg/mL were added ...
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TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring genebioRxiv - Genetics 2022Quote: ... To exclude cells with compromised membranes cell suspension was incubated with 7-aminoactinomycin D (7-AAD) (Thermo Fisher Scientific, A1310) in a ratio 1:1000 for at least 20 min ...
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bioRxiv - Cancer Biology 2023Quote: Caspase 3/7 activity was assessed using the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Scientific, #C10427) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... samples were incubated with 5 µg/mL of the DyLight488-conjugated anti-FLAG M1 antibody and the viability dye 7-Aminoactinomycin D (7-AAD, Invitrogen) for 45 min on ice and were then analyzed on a Guava easyCyte cytometer (Millipore) ...
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bioRxiv - Cell Biology 2021Quote: Intracellular levels of reactive oxygen species (ROS) were measured using the cell-permeant redox-sensitive probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Molecular Probes, Thermo Fisher Scientific, USA). HEK 293 cells were grown in a 96-well plate for fluorescent-based assays until ∼80% confluence ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Fecal samples were immediately transferred to an anaerobic chamber within 2 h after defecation and suspended at 20% (w/v) in reduced peptone water (0.1%, pH 7; Thermo Fisher Diagnostics AG, Pratteln, Switzerland). Immobilization of 10 ml fecal slurry in polymer gel beads (gellan gum (2.5% ...
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bioRxiv - Cancer Biology 2023Quote: ... Tris-Glycine 10-20% or Bis-Tris 4-12% SDS-PAGE gels were run at 4℃ and proteins were transferred onto nitrocellulose membranes using 15 Volts for 7 minutes via the iBlot-2 system (Thermo Fisher Scientific, Waltham, MA). The membrane was then blocked for 1 hour in LICOR Odyssey blocking buffer and incubated at 4℃ with the appropriate antibody overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with 2.5 µM DCFH-DA (2’,7’-dichloro-dihydro-fluorescein-diacetate; excitation at 495 nm, emission at 520 nm; Life Technologies, Europe BV, Stockholm, Sweden) in Hank’s buffered saline solution without phenol red for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... The pHrodo-TDM1 was obtained by conjugating the free lysine residues of TDM1 with the amine-reactive pH-sensitive pHrodo iFL Red STP ester dye (ThermoFisher Scientific, P36014) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... Covalent labeling of amine groups of the proteins with Alexa Fluor 488 or Alexa Fluor 647 succinimidyl ester dyes (Molecular probes/Invitrogen) was conducted in its polymer-assembled form as previously described (González et al ...
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bioRxiv - Immunology 2022Quote: ... and amine-reactive viability stained for 30 minutes at 4° C with Live/Dead Fixable Cell Stain Kit (#L34960, Thermo Fisher Scientific). Next ...
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bioRxiv - Immunology 2020Quote: ... Approximately 5 mg of purified ACE2 PD was covalently attached per 1 mL of 4% beaded agarose by amine coupling using AminoLink Plus resin (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2020Quote: ... All contrast agents were activated for bioconjugation with purified L-PGDS using by addition of 100mM EDC and 250mM sulfo-NHS dissolved in amine- and carboxyl-free MES buffer (Thermo Scientific™) with pH adjusted to 6.0 to a final ratio of 1:5 [50] ...
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bioRxiv - Microbiology 2022Quote: SpRecAA488 was made by covalently modifying primary amines (lysines or N-ter) of the protein with Alexa 488-succinidimyl ester (Molecular Probes, ThermoFisher), in presence of an excess of ssDNA (M13 mp18 ...
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bioRxiv - Bioengineering 2022Quote: ... The rat tail collagen I protein and GFOGER peptide peptide were subsequently blocked using methyl-PEG24-amine (MA-PEG24) (Thermo Fisher Scientific). Post functionalization ...
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bioRxiv - Cell Biology 2019Quote: ... on a QuantStudio 7 Flex instrument (Applied Biosystems). Relative gene expression was calculated using the ΔΔCq method and normalized to GAPDH expression ...
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bioRxiv - Molecular Biology 2020Quote: ... and QuantStudio 7 Flex thermal cycler (ThermoFisher; 4485701). Each qRT-PCR was repeated at least three times ...
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bioRxiv - Molecular Biology 2019Quote: ... MCF-7 cells were grown in DMEM (GIBCO) with phenol red ...
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bioRxiv - Neuroscience 2019Quote: ... and CellEvent caspase-3/7 reagent (ThermoFisher Scientific) were used according to manufacturer protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by incubation with 7-AAD (Thermo Fisher) for viability assessment ...