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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for 7 8 dimethylbenzo c acridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... HEK293S GnTI- cells were grown at 37°C with 8% carbon dioxide in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... 400 μl was incubated (15 min, 37 °C) with 8 μl MNase solution (>100 U/μl) (ThermoFisher). Reactions were stopped by adding EGTA (22.5 mM final ...
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bioRxiv - Genetics 2023Quote: ... The blocks were dissolved by treatment with 8 U Agarase (Thermo Fisher Scientific; at 42°C overnight). The DNA solutions were placed at RT and transferred to disposable reservoirs without pipetting ...
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bioRxiv - Biochemistry 2023Quote: ... total mRNA was randomly fragmented at 70°C for 8 min in fragmentation reagent (Thermo Fisher; AM8740) and size-selected for 50-90 nt fragments for constructing a sequencing library using Universal miRNA Cloning Linker (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... blotted at 8°C for 10 s at force 0 using a Mark IV Vitrobot (Thermo Fisher) and plunge-frozen in liquid ethane ...
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bioRxiv - Developmental Biology 2019Quote: ... 7% CO2 in a humidified incubator at 37°C and passaged by dissociation with Accutase (Thermo Fisher Scientific, A1110501) every 3-5 days ...
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bioRxiv - Microbiology 2021Quote: ... Parasitemia was monitored daily by flow cytometry by diluting 10 µl of each parasite culture well from each of two to three biological replicate samples into 200 µl of 1.0 µg/ml acridine orange (Invitrogen Life Technologies A3568) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... parasitemia was monitored daily by flow cytometry by diluting 10 μL of each parasite culture well from each of three biological replicates into 200 μL of 1.0 μg/mL acridine orange (Invitrogen Life Technologies A3568) in phosphate buffered saline (PBS ...
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bioRxiv - Physiology 2023Quote: ... For cell viability assays 7-AAD (7-Aminoactinomycin D, Invitrogen, A1310) and Hoechst 33342 (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... mice were sacrificed 24 h post exposure to Cl2 or air and lavaged as described previously(7, 8) Total protein concentrations in cell-free lavage were measured with the BCA Protein Assay Kit (#23225; Thermo Fisher Scientific, Rockford, IL). Cells were re-suspended in 100μl of phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... saline (N = 7) or muscimol (N = 8, conjugate of muscimol and the Bodipy TMR-X fluorophore, InvitrogenTM; purchased from Thermo Fisher Scientific, reference M23400) disolved in PBS (ph 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... were maintained at 37 °C in a humidified atmosphere of 8% CO2 and cultured in high-glucose Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... The injected individuals were kept at 18°C for 5 to 8 days in 6-well-plates (Nunc multidish no ...
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bioRxiv - Genetics 2023Quote: ... and72°C for 8 min for the final extension using a thermal cycler (GeneAmp 9700, Applied Biosystems, USA). The final PCR product was separated on 3% agarose gel containing 0.5μgethidium bromide in 1X TBE buffer and gel images were documented in the gel documentation system (Gene Genius ...
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bioRxiv - Microbiology 2023Quote: ... were cultured at 37°C with 8% CO2 while shaking at 130 rpm in ExpiCHO expression media (GIBCO). Vero E6-TMPRSS2 cells that stably express human TMPRSS2 (NIBIOHN ...
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bioRxiv - Cell Biology 2023Quote: ... after which the temperature was lowered to 16 °C and expression induced for 8 hrs using ITPG (Invitrogen). Cells were then collected by centrifugation at 4000 x g for 20 minutes and purified using Ni–NTA–agarose (Qiagen ...
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bioRxiv - Microbiology 2023Quote: Human 293F cells were maintained at 37°C with 5-8% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Molecular Biology 2019Quote: Huh-7 (human hepatoma) cells were maintained at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplemented with 10% (vol/vol ...
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bioRxiv - Cell Biology 2019Quote: ... COS-7 and U2OS cells were grown at 37°C with 5% CO2 in complete DMEM (Thermo Fisher Scientific, USA) containing 10% FBS and 1% L-glutamine ...
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bioRxiv - Biochemistry 2021Quote: ... cell pellets were incubated with 300 µL of 10 mg/mL lysozyme (prepared fresh in sterile H2O) and incubated for 7 minutes at 30°C/250 rpm to allow for efficient lysis by TRIzol reagent (Ambion). 10-15 glass beads (4mm diameter ...
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bioRxiv - Neuroscience 2020Quote: ... Eyes were first incubated in 1 mL of high glucose Hanks balanced salt solution/0.5 mg/ml proteinase K (37°C, 7 min) and then in Neurobasal medium (Life Technologies) plus 10% fetal calf serum to stop enzymatic activity ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial cultures were grown at 37°C either in M9 media [M9 salts (248510, Difco)] supplemented with 0.4% glucose (6363-53-7, Fisher Scientific), 1 mM MgSO4 (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... to final concentration 0.5 mg/ml protein and 6x dye and heated from 10 to 70 °C with fluorescence reading every 0.1 °C (4 to 5 sec) in a QuantStudioTM 7 Flex Real-Time PCR System (Life Technologies). Protein thermal melting curve were generated using the Protein Thermal Shift TM software ...
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bioRxiv - Physiology 2020Quote: ... and incubated in cell* dishes at 37° C for 4 – 7 hours before adding cell culture medium (RPMI 1640, -glucose +glutamine, Gibco), supplemented with penstrep (10,000 U/ml penicillin / 10,000 μg/ml streptomycin ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed and stained for 20 min at 4 °C with the following antibodies: anti-mouse CD8α APC-efluo780 (clone 53-6-7, ebioscience/ Thermofisher), anti-mouse CD8β AF700 or BUV495 (clone YTS156 ...
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bioRxiv - Neuroscience 2024Quote: ... The reaction was cycled at 58°C annealing temperature along with a melt curve analysis using a QuantStudio 7 Flex (ThermoFisher). For the telomere primers ...
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bioRxiv - Neuroscience 2024Quote: ... The reaction was cycled at 56°C annealing temperature along with a melt curve analysis using a QuantStudio 7 Flex (ThermoFisher). Each sample was run in duplicate ...
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bioRxiv - Biophysics 2021Quote: ... and DRAQ-7 (Thermofisher) was administered at 1:100 ratio to every sample droplet and the Petri dish was stored at room temperature for 10 min ...
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bioRxiv - Immunology 2022Quote: ... KLRG1 (PeCyanine 7, Invitrogen, FITC ...
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bioRxiv - Bioengineering 2020Quote: ... 7-AAD stain (Invitrogen) was added to each sample (2.5 μL per well in the 96-well plate ...
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bioRxiv - Synthetic Biology 2020Quote: ... pH = 7 (Invitrogen™) and at -30°C ...
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bioRxiv - Immunology 2020Quote: ... 7-Aminoactinomycin D (7-AAD) and SYTOX green were purchased from Thermo Fisher Scientific (Burlington ...
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bioRxiv - Cancer Biology 2023Quote: ... 7-AAD viability assays were carried out suing 7-AAD (Thermo Fisher, #A1310) where cells were harvested and washed twice in 1XPBS supplemented with 2% FBS (Gibco) ...
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bioRxiv - Molecular Biology 2021Quote: ... and the C-terminal foldon trimerization motif followed by an 8×His-tag was cloned into the pcDNA3.1(+) expression vector (Invitrogen). Furthermore ...
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bioRxiv - Microbiology 2020Quote: ... BS-C-1 cells were seeded into Nunc™ 8-well Lab-Tek II chambered coverglass slides (Thermo Scientific) to obtain 50 to 70% confluence on the day of tracking ...
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bioRxiv - Biophysics 2022Quote: ... at 37°C and 8% CO2 to a density of 3.5×106 cells/mL in FreeStyle suspension media (Gibco) supplemented with 2% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... Gels were polymerized for 2 h at 37°C and then digested with 8 U/ml Proteinase K (Invitrogen) in 50 mM Tris-HCl pH 8 ...
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bioRxiv - Immunology 2020Quote: ... fraction was separated by centrifugation at 10,000 x g for 30 minutes at 2-8 °C (Sorvall Lynx 6000, ThermoFisher). The IB pellet was washed with 20 mM HEPES (pH 8.0) ...
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bioRxiv - Biophysics 2021Quote: ... and incubated (95 °C, 20 min) before SDS-PAGE using 3–8 % Tris-Acetate protein gels (Thermo Fisher Scientific). After transfer onto PVDF membranes (iBlot 2 Dry Blotting System ...
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bioRxiv - Microbiology 2021Quote: Bone marrow derived macrophages (BMDMs) were generated from 6-8 week old BALB/c mice in RPMI-1640 (Gibco) containing 10% foetal calf serum ...
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bioRxiv - Immunology 2020Quote: ... cells were quickly lysed in 8% Lithium dodecyl sulfate LDS loading buffer preheated at 85°C (Invitrogen Cat#NP0007) and incubated with stirring at 500 rpm for 5 min at 85°C (Eppendorf ...
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bioRxiv - Biophysics 2023Quote: ... were grown at 37 °C and 8% CO2 to a density of 3.5 × 106 cells/mL in FreeStyle suspension media (Gibco) supplemented with 2% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... boiled at 95°C for 5 min and loaded into a NuPAGE 3-8% Tris-Acetate Gel (Thermo Scientific) along with HiMark™ Pre-stained Protein Standard (Thermo Scientific LC5699) ...
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bioRxiv - Immunology 2023Quote: ... Epithelial layer was removed by incubation (20 min, 37°C) in 10 ml HBSS (Ca-Mg-) containing EDTA (0.5M, pH 8, Invitrogen) and HEPES (1M ...
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bioRxiv - Physiology 2024Quote: ... maintained at 8°C and coupled to an Orbitrap ELITE™ Hybrid Ion Trap-Orbitrap Mass Spectrometer (ThermoFisher Scientific). 5 µL of extract were injected into an Agilent Poroshell 120 EC-C18 column (2.1 x 100 mm ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR was performed for 55-60 cycles for maximal saturation of signal with final extension at 72 °C for 7 min in a 7500 Fast Real-Time PCR System (Applied Biosystems). The primer sequences used were ...
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bioRxiv - Biophysics 2019Quote: ... The grids were blotted for 4 s with blot force 7 at 85% humidity and 15°C prior to plunge freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). Datasets were collected using a Titan Krios operated at an acceleration voltage of 300 keV and the GATAN K2 Summit direct electron detector coupled with the GIF quantum energy filter (Gatan Inc. ...
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bioRxiv - Immunology 2019Quote: ... and frozen down in 7% DMSO in fetal bovine serum and cooled at −1°C/min in a freezing container (Nalgene, ThermoFisher Scientific). Frozen PBMCs were stored at −80°C before shipping on dry ice to Benaroya Research Institute for further processing and stimulation as described above.
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bioRxiv - Immunology 2019Quote: ... one half being used immediately and the other half frozen in 7% DMSO in fetal bovine serum and cooled at −1°C/min in a freezing container (Nalgene, ThermoFisher Scientific). Freshly isolated PBMCs were plated in 96 deep-well plates (Greiner Bio-One ...
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bioRxiv - Immunology 2021Quote: ... BirA enzyme was added at a ratio of 1:200 w/w and incubated for 1 h at 37 °C before being buffer exchanged into PBS [pH 7.4] with a Zeba 7 kDa MWCO spin column (Thermo Fisher Scientific).35