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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for 7 8 dimethylbenzo c acridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and finally at 72 °C for 7 min using AmpliTaq Gold PCR Master Mix (Applied Biosystems) with template cDNA equivalent to 15 ng of total RNA and a high concentration (0.75 mM each ...
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bioRxiv - Cell Biology 2023Quote: ... COS-7 cells were grown at 37 °C under 5% of CO2 in DMEM Glutamax (Gibco) with 10% bovine fetal serum ...
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bioRxiv - Neuroscience 2024Quote: ... for 7 min at 37°C and the digestion was blocked with 10% FBS (#10500064, Gibco) in DMEM (#41966-029 ...
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bioRxiv - Neuroscience 2022Quote: Differentiated cells on day 15 (8 days suspension culture plus 7 days adherent culture) were harvested and dissociated using TrypLE Select (Gibco). Cells were then passed through a 20 µm strainer and resuspended in 1 x PBS with 0.04% BSA buffer ...
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bioRxiv - Neuroscience 2021Quote: Astrocytes at 3 DIV or at 7-8 DIV after lentivirus infection were loaded with 1 μg/ml Fura-2-AM (#F1221, ThermoFisher) in astrocyte culture medium for 30 min at 37 °C ...
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bioRxiv - Immunology 2020Quote: PBMC were seeded at a concentration of 7-8 × 106 cells/mL in RPMI 1640 medium without phenol red and glutamine (Gibco) supplemented with 10% heat-inactivated FBS (Gibco ...
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bioRxiv - Cell Biology 2022Quote: Lipid overlay assay was performed using PIP strips (nitrocellulose membrane spotted with 8 phosphoinositides and 7 other biologically relevant lipids) according to the manufacturer’s instructions (Thermo Scientific). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Treg cells were stimulated for 7-8 days with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Genetics 2023Quote: ... Quantitative real time PCR (n = 7–8 per genotype) was performed on an ABI 7300 Sequence Detection system (Applied Biosystems) using the PowerUp SYBR Green Master Mix Kit (Applied Biosystems).
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bioRxiv - Biochemistry 2024Quote: HEK293 cells with Fzd1/2/4/5/7/8 knocked out were provided by Michael Boutros (Voloshanenko et al., 2017) and maintained in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gemini) ...
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bioRxiv - Microbiology 2021Quote: Parasitemia in daily growth assays was measured via flow cytometry by incubating 10 µL of parasite culture with 190 µL of 0.4 µg/mL acridine orange (Invitrogen) in PBS for 1 minute ...
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bioRxiv - Neuroscience 2023Quote: ... Zebrafish embryos were injured at 3 dpf and placed in 5 ug/mL acridine orange (ThermoFisher, Cat. No. A1301) diluted in egg water for 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... and 7-AAD (7-Aminoactinomycin D) (Thermofisher, # A1310) was used for DNA staining ...
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bioRxiv - Molecular Biology 2020Quote: ... at 4°C for 80 min using a F37L-8×100 rotor (Thermo Fisher Scientific). The pelleted EVs were resuspended in 150 μL ice-cold DPBS with 1x phosphatase inhibitor / protease inhibitor solution ...
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bioRxiv - Molecular Biology 2021Quote: ... at 75°C for 8 minutes and immediately quenched with 10x Stop Reagent (AM8740, Invitrogen) and placed on ice to generate fragments 30-150 nucleotides in length.
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bioRxiv - Cell Biology 2020Quote: ... Sequencing was performed using Ion Proton instrument with 7 or 8 samples per chip with Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher Scientific). Reads were aligned to the hg19 AmpliSeq Transcriptome ERCC v1 with Torrent Mapping Alignment Program (version 5.0.4 ...
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bioRxiv - Biophysics 2023Quote: ... the media was removed and the cells were incubated with 8 µM CellEventTM Caspase 3-7 green detection reagent (Thermo Fisher) in PBS containing 5% FBS for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... The temperature was maintained at 37 °C by a thermostatic water circulator (NESLAB RTE-7; Thermo Scientific). The samples were prepared in 20mM HEPES ...
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bioRxiv - Immunology 2019Quote: ... for 1h at 37°C or 2 μM CellEvant CASPASE-3/7 Green detection reagent (Thermo Fisher) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Cell viability of the last hepatocyte enriched fraction was determined by acridine orange (AO)/ propidium iodide (PI) dual staining (Thermofisher) using a LUNA cell counting system (LabTech) ...
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bioRxiv - Immunology 2022Quote: BMDMs were plated at a density of 0.1×106 cells per well in their regular medium into black- walled 96-well plates and stained for 15 min with acridine orange (Invitrogen) according to manufacturers instructions ...
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bioRxiv - Microbiology 2020Quote: ... Parasitemia was monitored daily by flow cytometry by diluting 10 μl of each parasite culture well from 2-3 biological replicates into 200μl of 1.0 μg/ml acridine orange (Invitrogen Life Technologies A3568) in phosphate buffered saline (PBS ...
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bioRxiv - Genomics 2023Quote: ... Cell counts and viability were determined using a manual (Neubauer, Trypan Blue) or automated (Luna-FL, Dual Fluorescence Cell Counter, Logos BioSystems, Acridine Orange/PI, ThermoFisher) cell counting.
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bioRxiv - Microbiology 2024Quote: ... Parasitemia was monitored daily by flow cytometry by diluting 10 µL of each resuspended parasite culture well from each replicate into 200 µL of 1.0 µg/mL acridine orange (Invitrogen A3568) in phosphate buffered saline (PBS ...
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bioRxiv - Systems Biology 2023Quote: ... 30 s at 72 °C and a final step of 7 min at 72 °C using a GeneAmp 2400 (Applied Biosystems, Foster City, CA, USA) thermal cycler ...
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bioRxiv - Cell Biology 2022Quote: ... we used 7-AAD (7-Aminoactinomycin D) (Thermofisher, # A1310) or FxCycle™ PI/RNase Staining Solution (Thermofisher ...
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bioRxiv - Genomics 2020Quote: ... 7-Aminoactinomycin D (7-AAD) (1:200 dilution, ThermoFisher Scientific #A1310 ...
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bioRxiv - Neuroscience 2022Quote: ... 7-aminoactinomycin D (7-AAD, Thermo Fisher, A 1310) was added 1:50 as a cell death marker.
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bioRxiv - Immunology 2022Quote: ... for protein expression were maintained at 37°C and 8% CO2 in Expi293 Expression medium (GIBCO), transfected using Expi293 Expression System Kit (GIBCO ...
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bioRxiv - Immunology 2021Quote: ... were maintained at 37°C in 8% CO2 in ExpiCHO Expression Medium (Thermo Fisher Scientific, A2910002). Mycoplasma testing of cell lines was performed on monthly basis using a PCR-based mycoplasma detection kit (ATCC ...
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bioRxiv - Immunology 2020Quote: ... were maintained at 37°C in 8% CO2 in Expi293F expression medium (Thermo Fisher Scientific, A1435102).
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bioRxiv - Evolutionary Biology 2024Quote: ... BHK-21 cells and HCT-8 cells were cultured at 37 °C in DMEM (Gibco-Invitrogen) supplemented with 0.22% (w/v ...
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bioRxiv - Evolutionary Biology 2024Quote: ... BHK-21 cells and HCT-8 cells were cultured at 37 °C in DMEM (Gibco-Invitrogen) supplemented with 0.22% (w/v ...
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bioRxiv - Plant Biology 2019Quote: Total RNA was extracted from Arabidopsis leaf tissues grown under an 8-h photoperiod or cotyledons from etiolated seedlings grown in dark for 7 d by TRIzol (Thermo Fisher Scientific) followed by DNase treatment at 37 °C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... COS-7 cells (ATCC) were grown at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (Gibco) containing 10% fetal bovine serum and 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... prehybridization was carried out for 2 h at 42°C in 7 mL of prehybridization buffer ULTRAHyb (Ambion). Hybridization was performed overnight at 42°C in the same buffer in the presence of a [γ-32P]-labeled DNA oligonucleotide probe ...
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bioRxiv - Biochemistry 2023Quote: ... This solution was incubated for 1h at ~7°C with 0.25 ml of HisPur Cobalt resin (Thermo Scientific). The beads were allowed to settle to the bottom of a protein purification column and washed 5 times with 10 ml of washing buffer comprising 10 mM imidazole and 0.1% Triton X-100 in 2xPBS ...
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bioRxiv - Cancer Biology 2020Quote: Cells were washed 3 times with PBS and resuspended in RPMI 10% FBS containing 5ug/mL Acridine Orange (A3568, Invitrogen, Switzerland) to a concentration of 0.2 x 106 cells per mL ...
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bioRxiv - Microbiology 2021Quote: ... Parasitemia was monitored daily by flow cytometry by diluting 10 µl of each parasite culture well from each of two to three biological replicate samples into 200 µl of 1.0 µg/ml acridine orange (Invitrogen Life Technologies A3568) in phosphate buffered saline (PBS ...
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bioRxiv - Bioengineering 2021Quote: ... Cell counts and viability were assessed using acridine orange/propidium iodide (AO/PI) and a Countess Automated Cell Counter (Thermo Fisher). Media was added to cultures every 2 days to 3 days in a 3:1 ratio (new volume ...
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bioRxiv - Microbiology 2020Quote: ... Parasitemia was monitored daily by flow cytometry by diluting 10 μl of each parasite culture well from 2-3 biological replicates into 200μl of 1.0 μg/ml acridine orange (Invitrogen Life Technologies A3568) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... parasitemia was monitored daily by flow cytometry by diluting 10 μL of each parasite culture well from each of three biological replicates into 200 μL of 1.0 μg/mL acridine orange (Invitrogen Life Technologies A3568) in phosphate buffered saline (PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... 7-amino-actinomycin (7-AAD, ThermoFisher, for labeling nonviable cells) stained cells were sorted using a FACS Aria II sorter (BD BioSciences) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were stained with 7-amino-actinomycin (7-AAD, ThermoFisher) to label nonviable cells and then sorted using a FACS Aria II sorter (BD BioSciences ...
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bioRxiv - Immunology 2021Quote: ... 7-AAD (Invitrogen), Live/dead fixable viability dye (Invitrogen) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cover slips are mounted on top of a drop (7-8 uL) of ProLongTM Diamond Antifade Mountant (Thermo Fisher Scientific, Canada. Catalog# P36970).
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bioRxiv - Immunology 2022Quote: ... 2.7 μg envelope plasmid pMD2G and 8 μg target plasmid encoding shRNA or lentiCRISPRv2 encoding sgRNA using Lipofectamine® 2000 (Thermo Fisher Scientific). Lentivirus supernatants were collected at 36 h ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were washed then incubated in 8 μM of the ROS sensitive probe 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester (CM-H2DCFDA) (Molecular Probes, Eugene Oregon), for 45 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... HepG2 or MCF-7 cultures were grown for imaging on poly-D-Lysine coated 8-well LabTek II chambered coverglasses (Nunc, Rochester, NY, USA) for 1-2 days ...
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bioRxiv - Neuroscience 2021Quote: ... for 8 min at 37°C and later blocked using 0.5 mg/ml trypsin inhibitor (Gibco, R007100) and the suspension centrifuged for 5 min at 1000 rpm ...