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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for 7 Dimethylamino coumarine 4 Acetic Acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... using 7-AAD (ThermoFisher Scientific) as a dead stain.
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bioRxiv - Developmental Biology 2023Quote: ... using 7-AAD (ThermoFisher Scientific) as a dead cell stain ...
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bioRxiv - Bioengineering 2024Quote: ... 7 kDa MWCO (Thermo Scientific). The extent of biotinylation was measured using the QuantTag Biotin Quantification kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... buffered with 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technology, Thermo Fisher Scientific Inc., USA) and coated with 20 μg/mL laminin (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were decalcified in 10.6% buffered formic acid at 4°C overnight (Cal-Ex II, Thermo Fisher Scientific) and processed for histologic analysis ...
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bioRxiv - Bioengineering 2020Quote: ... Bodipy FL c16 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid, Thermofisher) was diluted in sterile RPMI-1640 (Corning ...
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bioRxiv - Immunology 2023Quote: ... at least 50 000 cells were seeded and incubated for 10min at 37°C with 2mM Bodipy-C11 (581/591) (4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid; Invitrogen) in PBS ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 10% Hi-FBS (Thermo Fisher Scientific). BSC-1 cells (ATTC ...
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bioRxiv - Biochemistry 2021Quote: ... Transfection of MCF-7 and MCF-7/ADR cells was performed with Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: Hematopoietic precursor cell-7 (HPC-7) cells were grown in IMDM medium (12440-053, Invitrogen) with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... and single cell suspensions stained with 7-aminoactinomycin D (7-AAD; ThermoFisher Scientific, Waltham, Massachusetts) were analyzed on an LSR II flow cytometer (Becton Dickinson).
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bioRxiv - Immunology 2020Quote: ... Cell death was assessed by 7-aminoactinomycin D (7-AAD, Affymetrix eBioscience, San Diego, CA) staining and flow-cytometry (FACSCantoII™ ...
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bioRxiv - Cancer Biology 2021Quote: Glucose uptake experiments were performed using 2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose) (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... mice were first anesthetized with a ketamine (100 mg/kg) and xylazine (7 mg/kg) mixture then perfused with 4% paraformaldehyde (Thermo Fisher Scientific), and the brains were extracted and cryoprotected in 30% sucrose in 1X PBS until they sank ...
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bioRxiv - Cancer Biology 2022Quote: ... culture medium was replaced with 100 µL full melanoma medium with 4 µM Caspase-3/7 activity dye (CellEvent, Thermo Fisher Scientific). Plates were then imaged on a Celigo Imaging Cytometer (Nexcelom ...
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bioRxiv - Microbiology 2021Quote: ... 7) and plunge-frozen at a condition of 95% humidity and 4°C by using a Vitrobot Mark IV (Thermo Fisher Scientific). The cryo-EM data were acquired with a Titan Krios at 300 kV (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... but the blastocysts were incubated in 10 μM 4-chloromethyl-6,8-difluoro-7-hydroxycoumarin (CMF2HC; Cell Tracker Blue, Life Technologies, Carlsbad, USA). Fluorescence intensity for GSH was measured under a Nikon scanning confocal microscope with a filter at 371 nm excitation and 464 nm emission ...
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bioRxiv - Immunology 2022Quote: The production of NO was evaluated using 4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate (DAF-FM DA)(D23844; Invitrogen, Waltham, MA). Following leukocyte isolation ...
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bioRxiv - Neuroscience 2021Quote: ... with the substrate tertiary-butyloxycarbonyl-leucine-methionine-7-amino-4-methylcoumarin (abbreviated to t-BOC-Leu-Met-CMAC, or in short: CMAC) (Invitrogen, Carlsbad, CA), at a concentration of 50 μM in the culture medium ...
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bioRxiv - Physiology 2021Quote: Total RNA from around 20 whole flies of both sexes at 4-7 days old was extracted using a PureLink RNA Mini Kit (Cat# 12183020, ThermoFisher Scientific, USA). Reverse transcription (RT ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Cell Biology 2020Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxy-d-glucose (2-NBDG) was purchased from Invitrogen (Carlsbad, CA, USA). Trypsin-EDTA solution was purchased from GIBCO BRL (Grand Island ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were separated by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in either homemade 7% or 4-12% gradient Bis-Tris polyacrylamide gel (Thermo Fisher Scientific) using 3-(N-morpholino)propanesulfonic acid (MOPS ...
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bioRxiv - Developmental Biology 2024Quote: Ovaries from 5-7 females were dissected in 1% PBS-Tween and fixed in a 1:1 mixture of 4% formaldehyde (Thermo Scientific, 28908) and heptane (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS pH 7.4 or pH 6), HA (Sigma-Aldrich, 9067-32-7, 0.5%–1% w/v in Hanks’ Balanced Salt Solution (Gibco, 14025-050) or Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cell Biology 2024Quote: ... The flow-through was then dried in a speed-vac and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Plant Biology 2022Quote: ... 0.5 mM aminocaproic acid and 30% sucrose) and loaded onto a 4-16% precast BN-PAGE gel (Life Technologies). Second dimension analysis was performed by solubilizing BN-PAGE gel slices in 2x Laemmli buffer (Laemmli ...
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bioRxiv - Microbiology 2021Quote: ... Previously pelleted spheroplasts were resuspended in 1 mL 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer solution (Gibco) and 100 μl were collected as whole spheroplasts ...
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bioRxiv - Microbiology 2020Quote: ... were quantified using an avidin and 4’-hydroxyazobenzene-2-carbocylic acid assay according to the manufacturer’s instructions (Fisher Scientific). Briefly ...
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bioRxiv - Bioengineering 2019Quote: ... C12 (4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid;D3823, Thermo Fisher Scientific), was added to the culture medium for a duration of 60 min and pumped through the media systems at 80 µl/h via positive pressure provided by a syringe pump ...
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bioRxiv - Molecular Biology 2020Quote: ... 1,1’-bi(4-anilino) naphthalenesulfonic acid (Bis-ANS) and Alexa Fluor® 488 phalloidin were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Immunology 2021Quote: ... Uptake of fatty acids was quantified after incubation with 1uM 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic acid (Bodipy-FL C16, Thermo Fisher) at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were acidified with 4 μL of 50 % formic acid and dried in a SpeedVac centrifugal evaporator (ThermoFisher) at 65°C for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... 4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (BODIPY-C12, Invitrogen D3835), was dissolved in 100% ethanol and conjugated to 10% bovine serum albumin ...
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bioRxiv - Bioengineering 2023Quote: PEGαMA hydrogels were made by dissolving the PEGαMA in pH 8.4 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Life Technologies) at 12.5-15.5 weight percent (wt%) ...
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bioRxiv - Cancer Biology 2023Quote: BODIPY FL C16 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid, Invitrogen D3821) was used to assess the ability of 3T3s to transport fatty acids into the cell from their surroundings ...
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bioRxiv - Cancer Biology 2019Quote: ... essential amino acids and non-essential amino acids (MEM Non-Essential Amino Acids Solution; Gibco, 11140050) (amino acid-only medium) ...
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bioRxiv - Immunology 2023Quote: ... Dead cells were excluded using 7-AAD (7-Aminoactinomycin D, Thermo Fisher Scientific, 00-6993-50). Cells were analyzed using a FACSCanto II flow cytometer (BD ...
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bioRxiv - Molecular Biology 2021Quote: ... in QuantStudio 7 Flex (Applied Biosystems), in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 7 μL of RNaseI (Invitrogen AM2249) was added to the clarified lysates and digestion was done for 1 h at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... on QuantStudio 7 Flex (Applied Biosystems). For quantification of gene expression ...
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bioRxiv - Bioengineering 2019Quote: ... 7′-dichlorodihydrofluorescein diacetate (cm-H2DCFDA, Invitrogen) for 60 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... or QuantStudio 7 Flex (Applied Biosystems). Refer to Supplemental Table 5 for primer sequences used.
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bioRxiv - Cell Biology 2020Quote: ... or QuantStudio 7 Flex (Applied Biosystems). All mRNA expression levels were calculated relative to the housekeeping gene ...
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bioRxiv - Synthetic Biology 2021Quote: ... 7% KnockOut SR (vol/vol) (Gibco), 2 mM L-glutamine ...
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bioRxiv - Developmental Biology 2020Quote: ... 7% KnockOut SR (vol/vol) (Gibco), 2mM L-glutamine ...
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bioRxiv - Immunology 2021Quote: ... with 7% heat-inactivated FBS (Invitrogen) and 1X penicillin-streptomycin (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... program #3 for 7 min (ThermoFisher). The membrane was saturated by incubation in PBS (without EDTA ...