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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for 7 Dimethylamino coumarine 4 Acetic Acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 500 mM 6-aminohexanoic acid) was added to lysates right before loading samples into a NativePAGE Bis-Tris gel 4-16% (Life Technologies). The proteins were separated according to Wittig et al.32 and transferred onto a PVDF membrane using a standard Tris-glycine transfer buffer with 0.05% SDS ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were incubated overnight in a humidified chamber at 4 °C in TBS-T with 1% Fatty acid free-BSA (Fisher Scientific) with anti-mouse/human antibodies ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were incubated for 20 min at 37°C in PBS containing 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16; 1 μM; Thermo Fisher Scientific). To determine neutral lipid content ...
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bioRxiv - Immunology 2022Quote: ... for 20 min before incubation overnight at 4°C in PBS-BR containing any of the following mAbs or reagents: DAPI nucleic acid (Molecular Probes), phalloidin Alexa fluor 488 (Molecular Probes) ...
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bioRxiv - Cancer Biology 2022Quote: ... The stock solutions of the agonists were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific) and the enzyme inhibitors were reconstituted according to manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were cleared by centrifugation at 21,000 x g for 10 minutes at 4°C and protein concentration was determined by Bicinchoninic acid assay (Pierce, ThermoFisher Scientific). Proteins were reduced with 5 mM dithiothreitol for 30 minutes at 55°C ...
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bioRxiv - Cell Biology 2023Quote: Peptides were dissolved in 4 μL 0.2% (v/v) formic acid (FA) and injected into an Easy-nLC 1200 (Thermo Fisher Scientific). Peptides were separated on a 15-cm in-house packed column (360 μm OD × 75 μm ID ...
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bioRxiv - Immunology 2023Quote: ... The WIV was then dialyzed against 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-buffered saline (Thermo Fisher Scientific, Bleiswijk, the Netherlands) at 4°C overnight to remove β-propiolactone ...
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bioRxiv - Immunology 2023Quote: ... supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4 mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific) cultured for 4-7 days at 8% CO2 saturation and 37°C with shaking ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were dissolved in 10 µL of reconstitution buffer (96:4 water: acetonitrile, 1% formic acid and analyzed by LC-MS/MS using QExactive (Thermo Fisher) in the proteomics core facility at EMBL Heidelberg (https://www.embl.org/groups/proteomics/).
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bioRxiv - Immunology 2023Quote: ... supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4 mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... 20% volume of 1M 2-morpholin-4-ylethanesulfonic acid (MES) pH 5 and Immobilized Protein G resin (Thermo Scientific Prod#20397) were added to supernatant and sample was incubated overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lot 134874), and 4-(2-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, purity ≥ 99.%, CAT #BP310-1, Lot 052975) were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were dissolved in 10 µL of reconstitution buffer (96:4 water: acetonitrile, 1% formic acid and analyzed by LC-MS/MS using QExactive (Thermo Fisher) in the proteomics core facility at EMBL Heidelberg (https://www.embl.org/groups/proteomics/) ...
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bioRxiv - Cell Biology 2024Quote: ... as described above and separated using a linear 12.5 min gradient of 4-32% aqueous acetonitrile versus 0.1% formic acid on a nanoflow chromatography system (Ultimate nanoRSLC, Thermo Fisher Scientific) using a reversed phase-C18 column (PepSep Fifteen ...
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bioRxiv - Cell Biology 2020Quote: ... using the ViiAtm 7 platform (Applied Biosystems). Relative quantification was conducted by either SYBRgreen fluorescent dye (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... 7 kDa cut-off (Thermo Scientific, #89890). The protein concentration was adjusted to 0.5 mg/ml in CD buffer (250 μL) ...
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bioRxiv - Genomics 2020Quote: ... 7 mM MgCl2 (Ambion, Cat. No. AM9530G), 10% N,N-dimethylformamide (Sigma ...
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bioRxiv - Immunology 2022Quote: ... on the ViiA 7 system (Applied Biosystems). PCR of CD19 was used for normalization ...
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bioRxiv - Immunology 2022Quote: ... on the ViiA 7 system (Applied Biosystems) as described (62 ...
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bioRxiv - Molecular Biology 2021Quote: ... 7 µL HinFI (10U/µL, Thermo Scientific)) per 70 µL of cDNA and digested at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and a QuantStudio 7 Flex (Life Technologies) instrument ...
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bioRxiv - Microbiology 2021Quote: ... QuantStudio 6/7 Pro systems (Applied Biosystems). The following primers were used ...
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bioRxiv - Neuroscience 2020Quote: ... with a ViiA 7 System (Applied BioSystems). The UBE2D2 gene transcript was used as the internal reference ...
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bioRxiv - Systems Biology 2021Quote: ... and 1:1000 7-AAD (ThermoFisher Scientific). Mouse IgG2A-488 (R&D systems ...
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bioRxiv - Microbiology 2020Quote: ... and a QuantStudio 7 Flex (Life Technologies) instrument for Il17a (96) ...
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bioRxiv - Immunology 2022Quote: ... and 7-AAD (Invitrogen, 00-6993-50). CD19/CD20+ ...
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bioRxiv - Neuroscience 2022Quote: ... Potassium Chloride (Fisher Scientific- 7447-40-7) and Calcium chloride (Fisher Scientific- 10043-52-4).
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bioRxiv - Neuroscience 2022Quote: ... for 7 days in Neurobasal medium (Invitrogen) with 2% B-27 supplement (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... 7 mL of DMEM (Gibco, #11995-073) with 630 U/mL Type 1A collagenase from clostridium histolyticum (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR (Applied Biosystems, #ViiA 7) was performed using the KAPA SYBR FAST qPCR master Mix (KAPA Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... CellEvent Caspase-3/7 Detection Reagent (Invitrogen) and Hoescht were added to the media at concentrations listed in S4 Table and incubated for 30min at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2019Quote: ... IL-7 (5 ng ml−1; ThermoFisher), and IL-15 (5 ng ml−1 ...
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bioRxiv - Cell Biology 2020Quote: ... CellEvent Caspase-3/7 (Life Technologies C10723), Fis1 polyclonal antibody (Proteintech 109561-AP) ...
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bioRxiv - Neuroscience 2021Quote: ... HT-7 (1:200, #MN1000, Thermo Scientific), GPC-4 (1:200 ...
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bioRxiv - Biochemistry 2020Quote: ... 7-hydroxycoumarin was obtained from Acros Organics, a part of Thermo Fisher Scientific (New Jersey ...
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bioRxiv - Bioengineering 2019Quote: ... IL-7 at 5 ng/mL (ThermoFisher), and IL-15 at 5 ng/mL (ThermoFischer) ...
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bioRxiv - Cell Biology 2020Quote: ... on QuantStudio 7 Flex system (Life Technologies). To quantify viral genomes ...
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bioRxiv - Cell Biology 2020Quote: ... Claudin 7 (Fisher Scientific 10537403, 1:200), Large T (Santa Cruz sc-20800 ...
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bioRxiv - Neuroscience 2021Quote: ... or QuantStudio 7 Pro SmartStart (Applied Biosystems) real-time PCR system ...
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bioRxiv - Neuroscience 2021Quote: ... on ABI7900RH or QuantStudio 7 (Applied Biosystems) or LightCycler® 480 (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... and 7-Amino-Actinomycin D (7AAD, Invitrogen) for live-dead staining were incubated in 100-200µL FACS Buffer for 30min on ice in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... in a QuantStudio 7 Flex (Applied Biosystems). Gene expression was quantified in Microsoft Excel 365 as relative expression ratio using primer efficiencies calculated by a relative standard curve ...
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bioRxiv - Molecular Biology 2023Quote: ... 7 μL of Dynabeads Protein G (Invitrogen) was added to the mixture and incubated at 4ºC for 40 min ...
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bioRxiv - Neuroscience 2022Quote: ... in QuantStudio 7 Flex (Applied Biosystems, ABI) machine ...
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bioRxiv - Physiology 2023Quote: ... 7-AAD (Thermo Fisher Scientific, Massachusetts, USA) was added into the medium before sorting on MoFlo Astrios (Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... anti-Thy1.1 (1:500, OX-7; Invitrogen) anti-CD4 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... The QuantStudio 7 Flex cycler (Thermo Fisher) was used for amplification ...
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bioRxiv - Microbiology 2023Quote: ... with the Quantstudio 7 Flex (Life Technologies) to quantitate relative levels of transcriptional activation or viral RNA compared to the house keeping gene HPRT1 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were stained with 7-AAD (Thermofisher), and live cells were collected using BD FACSMelody™ Cell Sorter (BD Biosciences) ...