Labshake search
Citations for Thermo Fisher :
551 - 600 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... The epidermis was peeled off and discarded and the dermis was minced into small pieces and digested at 37°C for 1 h following 4°C for overnight using collagenase IV (17104019, Gibco). The resulting cell suspension was filtered through a 70 μm cell strainer (BD Falcon) ...
-
bioRxiv - Biophysics 2024Quote: ... The supernatant was incubated rotating for 1 h at 4 ºC on 1.25 mL bed of HisPur Ni-NTA agarose beads (ThermoFisher Scientific), pre-equilibrated in lysis buffer ...
-
bioRxiv - Cell Biology 2021Quote: ... washed 3 times and incubated with 4’,6-Diamidino-2-phenylindole (DAPI; 2mg/ml) (Invitrogen) in PBS for 5 minutes ...
-
bioRxiv - Cell Biology 2020Quote: ... After washing and nuclear counterstaining with 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher, 3 µM), sections were mounted on microscopic slides using Aqua Poly/Mount (Polysciences) ...
-
bioRxiv - Bioengineering 2023Quote: ... in PBS pH 7.4 or pH 6), HA (Sigma-Aldrich, 9067-32-7, 0.5%–1% w/v in Hanks’ Balanced Salt Solution (Gibco, 14025-050) or Dulbecco’s modified Eagle’s medium (DMEM ...
-
bioRxiv - Molecular Biology 2022Quote: ... medium (1:1) supplemented with N-2 (Gibco), B-27 (Gibco) ...
-
bioRxiv - Biochemistry 2023Quote: ... NaHCO3 and 150 μL acetone containing 4 mg/mL 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide (L-FDAA, Thermo Scientific) was added ...
-
bioRxiv - Neuroscience 2020Quote: ... The sections were then incubated with the following secondary antibodies for 3 h at room temperature: anti-rabbit IgG-Alexa488 and anti-rat IgG-Alexa488 (1:1000 for both; Invitrogen Co). Sections were mounted on microscope slides and embedded with Vectashield that includes DAPI for nuclear staining (Vector Laboratories ...
-
bioRxiv - Immunology 2021Quote: ... or CA-074me for 1 h before challenge with bacteria were washed 3 times with pre-warmed Hanks’ Balanced Salt Solution (HBSS, Thermo-Fisher Scientific) and mROS were stained using 2.0 µM MitoSOX Red (Life Technologies ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were incubated at room temperature for 3 h in TBS containing AlexaFluor-546-conjugated donkey anti-goat IgG secondary antibody (1:400, A-11056, Fisher Scientific), 0.5% Triton X-100 ...
-
bioRxiv - Cell Biology 2023Quote: ... Secondary antibody incubation was for 3 h with Alexa Fluor 647 goat anti-mouse IgG (1:1000, Thermo Fisher Scientific, A21236) and Alexa Fluor 488 goat anti-rabbit IgG (1:1000 ...
-
bioRxiv - Microbiology 2020Quote: ... Chromatin was reverse crosslinked by incubation at 68°C for 2 h and purified by phenol:chloroform:isoamyl alcohol 25:24:1 (Life Technologies) extraction and ethanol precipitation ...
-
bioRxiv - Neuroscience 2021Quote: ... or Cy3-labeled species-specific secondary antibodies for 2 h at a dilution of 1:200 (Invitrogen; Jackson ImmunoResearch). To avoid cross talk between multiple fluorophores ...
-
bioRxiv - Neuroscience 2020Quote: ... before 2-h incubation at room temperature with a secondary antibody Alexa 555 made in rabbit (Invitrogen, 1:500). After several washes ...
-
bioRxiv - Microbiology 2021Quote: ... 1 μg of linearised plasmid was incubated for 2 h at 37°C with 1U T7 RNA polymerase (ThermoFisher), 5 mM DTT ...
-
bioRxiv - Microbiology 2021Quote: ... RNA was treated 1 h at 37 °C with 2 units of Turbo DNAse (ThermoFisher Scientific, Waltham, MA, USA), purified using NucleoSpin RNA Clean-up (Macherey-Nagel ...
-
bioRxiv - Microbiology 2021Quote: ... followed by 2 h staining with secondary donkey anti-mouse Alexa Fluor 546 antibody (Invitrogen, A10036, 1:500 dilution) and 1μg/ml DAPI.
-
Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... sections were washed and incubated for 2 h with secondary antibodies conjugated to Alexa-488 (1:2000; Molecular Probes, Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by 2 h incubation with fluorescent secondary antibodies (Fluor 568 labeled donkey anti-mouse (1:500; Life Technologies), Fluor 488 labeled donkey anti-rabbit (1:500 ...
-
bioRxiv - Neuroscience 2021Quote: ... Membranes were subsequently incubated in blocking buffer containing HRP-conjugated anti-mouse IgG (H+L) (1:2000, Fisher 62-6520) or HRP-conjugated anti-rabbit IgG (H+L) (1:2000, Life Technologies 31462) for one hour ...
-
bioRxiv - Immunology 2020Quote: ... They were then exposed at room temperature first for 1 h to primary antibodies in blocking buffer and then for 1 h to Alexa Fluor–conjugated secondary antibodies (Thermo Fisher Scientific) and Hoechst 33342 in blocking buffer ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by a 1 h incubation with goat anti-rabbit IgG (H+L) conjugated with Alexa fluor 488 (A32731; 1:1000; Invitrogen, Waltham, MA). The sections were then incubated with GFAP antibody (mouse anti-GFAP ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were washed in PBS and then incubated for 1 h in 1:500 diluted goat anti-chicken IgG (H+L) cross-adsorbed secondary antibody (Alexa fluor 488; Fisher Scientific A11039). The mounted slices were imaged using a Leica SP8 X confocal microscope equipped with a 63x 1.3 NA glycerol immersion objective.
-
bioRxiv - Cell Biology 2020Quote: ... The pellet was resuspended in 1xPBS supplemented with 2 % fetal bovine serum (FBS, Hybclone) and 1 μg/mL 7-AAD (Life Technologies), filtered through a 40 μm strainer ...
-
bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 isolate Wuhan-Hu-1 (GenBank accession #: MN908947) at a 4:1 ratio by lipofectamine 3000 (Thermo Fisher Scientific). After 48 to 72 hours ...
-
bioRxiv - Zoology 2023Quote: ... stained with 4′,6-diamidino-2-phenylindole (DAPI) (1:2000 dilution) and Alexa Fluor-conjugated donkey-anti-mouse (Molecular Probes, 1:1000), in PBST at room temperature for 2 hours ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were passaged 1:4 into new six-well plates with complete DMEM containing 2 μg mL-1 puromycin (Thermo Fisher Scientific). After selection for two weeks ...
-
bioRxiv - Zoology 2024Quote: ... Tissue samples were subsequently rinsed three times with DPBS again and then transferred onto microscope slides in mounting buffer containing 1:1 DPBS: glycerol and 1µg/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Molecular Probes, Eugene, OR) to stain cell nuclei in prepared tissues and examined under a Lumen Dynamics XCite™ 120Q Nikon fluorescence microscope (Nikon ...
-
bioRxiv - Neuroscience 2020Quote: ... precoated for 1 h with geltrex (Life Technologies A1413302), and fed twice-weekly for two weeks with 150 µL microglial medium ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 µl Maxima H Minus RT (Thermo Scientific. EP0742) and nuclease-free water up to 20 µl ...
-
bioRxiv - Genetics 2020Quote: ... and 1 µL RNase H (Thermo Fisher, #18021-071).
-
bioRxiv - Microbiology 2022Quote: ... for 1 h and 10 μg/ml DAPI (Invitrogen) for 1 min ...
-
bioRxiv - Cell Biology 2023Quote: ... Anti-rabbit IgG (H+L) 1:5000 (Invitrogen, G21234), Anti-mouse IgG 1:5000 (Invitrogen ...
-
bioRxiv - Microbiology 2023Quote: ... 1:2,000 (Invitrogen; Rabbit anti-Mouse IgG (H+L) Secondary Antibody ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... the coverslips were incubated 1 h with mouse anti–V5 antibody (Invitrogen, 1:500) and 1 h with the secondary antibody which was donkey anti–mouse coupled to Alexa Fluor 488 (Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: ... mouse anticlaudin 7 (Invitrogen Cat#37-4800, 1:100 for IF); rabbit anti-ARPC2 (Abcam Cat#ab133315 ...
-
bioRxiv - Genomics 2023Quote: ... cells were resuspended at 1 × 10^7 in 90% FBS (ThermoFisher) 10% DMSO (Sigma Aldrich) ...
-
bioRxiv - Immunology 2023Quote: ... Buffy coats were diluted 1:7 with Dulbecco’s PBS (Life Technologies). PBMC were obtained by density gradient centrifugation at 2000 rpm for 20 min at room temperature with 35 mL cell suspension stacked on 15 mL Biocoll separation solution (Biochrom) ...
-
bioRxiv - Neuroscience 2024Quote: ... AlexaFluor 568 goat anti mouse (Invitrogen, A11004, 7 RRID:AB_2534072, 1:2000), AlexaFluor 568 anti rabbit (Invitrogen ...
-
bioRxiv - Neuroscience 2020Quote: ... and incubated overnight in mouse anti-Hu primary antibody at 4°C (1:200 in 3% block; Invitrogen). After three 5-min PB rinses at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... 4) counterstained with the nuclear dye TO-PRO-3 (diluted 1:10’000; Life Technologies, Inc., Gaithersburg, MD, USA). Finally ...
-
bioRxiv - Microbiology 2021Quote: ... Paris) and pCMV-VSV-G at a ratio of 4:3:1 with Lipofectamine 3,000 (Thermo Fisher Scientific). Supernatants were collected 48 h after transfection ...
-
bioRxiv - Physiology 2022Quote: ... The samples were combined with 1 ml of 3:13 dilution of Erlich’s reagent [1.5 g of 4- (dimethylamino) benzaldehyde (ThermoFisher); 5 ml ethanol ...
-
bioRxiv - Bioengineering 2023Quote: ... pRSV-Rev = 5: 3: 2) were co-transfected into HEK-293T cells at a ratio of 1:1 using lipofectamine 3000 (Invitrogen, USA, Cat: #L3000015). After 48 h ...
-
bioRxiv - Neuroscience 2023Quote: ... An imaging window was constructed from three layers of microscope cover glass (1 x 5 mm, 2 x 3 mm diameter, Fisher Scientific, no. 1) joined with a UV-curable optical glue (NOR-61 ...
-
bioRxiv - Cancer Biology 2022Quote: ... culture medium was replaced with 100 µL full melanoma medium with 4 µM Caspase-3/7 activity dye (CellEvent, Thermo Fisher Scientific). Plates were then imaged on a Celigo Imaging Cytometer (Nexcelom ...
-
bioRxiv - Developmental Biology 2019Quote: ... and the rest was immunoprecipitated at 4°C for 2 h using Dynabeads Protein A (Life Technologies, # 10008D)-conjugated anti-m6A antibody (Synaptic systems ...
-
bioRxiv - Biochemistry 2022Quote: ... and incubated for 2 h at 4°C with 50 µl of IgG magnetic PanMouse Dynabeads (Thermo Fisher) equilibrated with IP buffer ...
-
bioRxiv - Neuroscience 2021Quote: ... slices were incubated with 4’,6-diaminodino-2-phenylindole (DAPI, Life Technologies D-21490, 1:2000) for 15 min ...
-
bioRxiv - Neuroscience 2020Quote: ... The nuclear dye 4’,6-diamidino-2-phenylindole (DAPI) at 1 l ng/ml (Molecular Probes) was added to visualise all cells ...