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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells were all pulsed for 1 h and chased for 1 h with either with 100 µg/mL Alexa488-conjugated dextran (ThermoFisher) or 50 µg/mL Alexa546-dextran (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were subsequently incubated in blocking buffer containing horseradish peroxidase (HRP)-conjugated anti-mouse IgG (H+L) (1:5,000, Fisher 62-6520) or HRP-conjugated anti-rabbit IgG (H+L) (1:5,000, Life Technologies 31462) for 1 h ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco #25300-054). The HEK293FT cell line tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).
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bioRxiv - Cell Biology 2022Quote: ... HCEnCs were rinsed in DPBS and passaged at a ratio of 1:2 or 1:3 with TrypLE (Thermo Fisher Scientific) for 10-15 min at 37°C in 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: FOs from 2 or 3 independent differentiations were fixed using 4% paraformaldehyde (Thermo Scientific) in PBS (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... The samples were then rinsed with PBS (5 × 3 min) and incubated for 1 h at 37°C with the following secondary antibodies: anti-human Alexa 488 (Thermo Fisher, Waltham, MA, USA; 1:750) or anti-mouse Alexa 488 (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: Apoptosis was measured by caspase-3/7 staining using CellEvent® Caspase-3/7 Green ReadyProbes® Reagent (ThermoFisher Scientific Inc., cat# R37111). CHLA20 and NGP cells were grown until confluence on 6-well plates with six days of treatment with DMSO or GSK591 ...
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bioRxiv - Cancer Biology 2024Quote: ... 48hrs after transfection the cells were fixed and stained for activated Caspase-3/7 using Apoptosis CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) according to manufacturers’ instructions ...
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bioRxiv - Microbiology 2023Quote: Cultured viruses were collected by centrifugation at 9,000 rpm for 1 h at 4°C (Sorvall ST8FR, Thermo Scientific) and resuspended in phosphate-buffered saline ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were incubated in shake tubes over 1 h at 4°C with recombinant protein G–Sepharose (Invitrogen). The Sepharose was washed with RIPA lysis buffer and eluates were obtained through an addition of Laemmli Buffer.
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bioRxiv - Biochemistry 2024Quote: ... The lysate was centrifuged (158,000 g, 4 °C, 1 h) and the cleared lysate incubated with Ni-NTA agarose (Invitrogen) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Hoescht (1:1000-2000) (Molecular Probes, #H-3570).
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bioRxiv - Microbiology 2023Quote: ... for 1 h and washed with PBS (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... 1 μL of RNase H (Invitrogen, Cat. # 18021071) was added and the reaction was incubated at 16°C for 30 mins ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR on the mouse Zdhhc17 gene using primers spanning exons 1 and 2 (5’-ACCCGGAGGAAATCAAACCACAGA-3’ and 5’-TACATCGTAACCCGCTTCCACCAA-3’) and Sso/Advanced Universal SYBR green supermix (Fisher Scientific) was performed on CFX96 Real Time System (C1000 Touch Thermal Cycler ...
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bioRxiv - Cell Biology 2023Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Peptides were incubated for 1 h at 22°C in the presence of a 2:1 mass ratio of TMT10plex reagent (Life Technologies cat. no. 90111) to peptides ...
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bioRxiv - Biochemistry 2022Quote: ... and probed for 2 h with an FITC-conjugated goat anti-rat antibody (1:300, Thermo Fisher Scientific). VE-cadherin in the superficial vascular plexus and deep capillary plexus was visualized in the retinas by confocal microscopy as illustrated in Fig ...
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bioRxiv - Immunology 2020Quote: ... and mouse anti-human HLA-DR antibody (Thermo Fisher Scientific, USA, 1:100, 2 h at 37°C). Also ...
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bioRxiv - Cell Biology 2021Quote: ... for 1–2 h at 37°C followed by a 10 min incubation with Accutase (Thermo Fisher Scientific) at 37°C to obtain a single cell suspension ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h with donkey anti-rabbit Alexa Fluor488 (1:200, ThermoFisher Scientific, A-21206) in 1% FBS PBS-Tx ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 1 h in anti-HA 2-2.2.14 IgG1 mouse mAb (ThermoFisher Catalog# 26183; RRID:AB_10978021) diluted 1:2000 and following 3 x 10 min washes in 0.1 M PB ...
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bioRxiv - Physiology 2023Quote: ... sections were incubated 2 h at RT with A568 donkey anti-rabbit secondary antibody (1:500; Invitrogen; A10042) in [PB 0.2% Tx + 1% donkey serum] ...
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bioRxiv - Microbiology 2021Quote: ... The plate was washed 3 times before adding 50 μl of 1:1,000 diluted Goat anti-Mouse IgG H+L-HRP antibody (Invitrogen, # 31430) to the plate and incubated for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... for 48 h then transfected for 24 h with 1 ug His-G3BP1 3’UTR constructs using Lipofectamine LTX and Plus reagent (Invitrogen). To determine mRNA stability ...
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bioRxiv - Neuroscience 2021Quote: Astrocytes at 3 DIV or at 7-8 DIV after lentivirus infection were loaded with 1 μg/ml Fura-2-AM (#F1221, ThermoFisher) in astrocyte culture medium for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids developed within 7 - 21 days after initial seeding and were first passaged (1:2) by dissociation using TrypLE Express Enzyme (ThermoFisher) and resuspension in OcellO primary organoid medium ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were treated with aptamers at reported concentrations or with vehicle (DPBS and Mg2+) for 48 h and then stained according to the CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (ThermoFisher Scientific, cat: #C10427). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... stained with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Grand Island, NY, USA) for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... washed in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Thermo Fisher Scientific, Waltham, MA, USA) two times ...
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bioRxiv - Bioengineering 2020Quote: ... then incubated in 4’,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Carlsbad, CA, USA) for 10 minutes and washed with PBS again ...
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bioRxiv - Bioengineering 2022Quote: ... cell nuclei were stained with 1:1000 DAPI (4′,6-Diamidino-2-Phenylindole, Dihydrochloride, ThermoFisher) in PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000, Thermo Fisher Scientific). Alexa Fluor 568 phalloidin was used to stain filamentous actin (F-actin ...
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were stained with 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes). Antibodies are listed in the Key Resources Table ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were stained with 4’,6-diamidino-2-phenylindole (DAPI, 1:10K, Thermo Fisher Scientific) and mounted onto Superfrost slides (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and all sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher, 1:1000).
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bioRxiv - Immunology 2020Quote: ... the sections were stained with DAPI (4′, 6-diamidino-2-phenylindole; 1 μg/ml; Invitrogen). In the immunofluorescence study ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclear staining was done with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000; Thermo Fisher) for 5 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000; Invitrogen, Eugene, OR). The sections were mounted onto gelatinized slides and left to dry (at least 2 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... 1/4 concentration of both inhibitors and is supplemented with 2% ESC-qualified FBS (Gibco). After day 2 ...
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bioRxiv - Bioengineering 2023Quote: ... followed by incubation with 2 μM calcein-AM and 4 μM ethidium homodimer-1 (Invitrogen) for 30 min to examine viability ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then counterstained with 4’,6-diamidino-2-phenylindole (DAPI) (D1306, 1:10,000, Invitrogen) diluted in 1X PBST (1:10,000) ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated in 4′,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Carlsbad, CA, USA) for 10 minutes and washed with PBS again ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissues were then stained with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, D1306, 1:5000) for five minutes to visualize DNA ...
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bioRxiv - Genetics 2024Quote: ... The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (1 μg/mL, Invitrogen) for 5 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... secondary antibody incubation was performed for 1 h with 1× streptavidin-HRP (Invitrogen) and goat anti-rabbit Alexa Fluor 488 (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... for 1 h at 10 V in 1×transfer buffer (Thermo Scientific BT00061) and probed with a primary mouse anti-HA antibody (Sigma Aldrich H9658 ...
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bioRxiv - Microbiology 2020Quote: ... The cleared lysate was incubated for 2 h at 4 °C with Ni-NTA agarose resin (Invitrogen) under slight agitation ...
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bioRxiv - Immunology 2023Quote: ... 1 uM APG-4 (pre-incubated 1:1 with Pluronic-127, Invitrogen) and K+-permeable ionophores valinomycin ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-rabbit Alexa Fluor 488 IgG H+L (1:400, A11008) and goat anti-mouse Alexa Fluor 568 IgG H+L (1:250, A11004) (Invitrogen, ThermoFisher).