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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for 6 Oxabicyclo 3.1.0 hexane 3 carboxylicacid ethylester 1α 3α 5α 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: The PPMI iPSC lines were thawed and grown on matrigel (Corning)-coated plates with Essential 8 Flex (E8, Batches 1, 2 and 3) or Essential 6 (E6, Batches 4 and 5) media (both Gibco) for about one month (5 passages) ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was extracted from brain punches as well as HBEC- 5i and bEnd.3 cells in 6-well plates using TRIzol (Invitrogen) homogenization and phase separation with chloroform ...
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bioRxiv - Biochemistry 2023Quote: ... 3 x 5 min) on a rocking platform and stained with 4′,6-diamidino-2- phenylindole dihydrochloride (DAPI) (Life Technologies) staining (2 μM ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 10 to 20 ovaries from 3-6 day-old flies using TRizol (Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... 20 ng/mL TPO and 40 ng/mL IL-3 from day 1 to day 6 with a media refresh every 2 days (all cytokines from Thermofisher). On day 8 ...
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bioRxiv - Cell Biology 2023Quote: ... siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’, 5’-GGGAUGAUGAGACCAUGUAtt-3’, 5’- AAAUCCUAAUGGAGACAAAtt-3’, Ambion)
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bioRxiv - Cancer Biology 2021Quote: ... Culture medium was refreshed every 2–3 days and organoids were passaged 1:2–1:6 every 7–21 days using TrypLE Express (Thermo Fisher). For co-culturing ...
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bioRxiv - Bioengineering 2022Quote: Primary neonatal cardiomyocytes were isolated from C57BL/6 mouse neonates on postnatal days 3-5 using the Pierce Primary Cardiomyocyte Isolation Kit (Thermo Fisher) as previously described (14) ...
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bioRxiv - Developmental Biology 2019Quote: ... blastocysts were collected from uterine horns and put on culture for 3-6 days in ES derivation medium composed of GlutaMAX/DMEM (Gibco, 31966), 15% FBS (Biowest) ...
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bioRxiv - Cell Biology 2019Quote: The staining of limiting membrane of the yeast vacuole was achieved with FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Invitrogen). Cells were grown to early-log phase in appropriate medium ...
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bioRxiv - Systems Biology 2019Quote: A mate-pair library with 3-6 kb fragments was prepared from pure genomic DNA using Ion TrueMate Library Reagents (Life Technologies). The Ion PGM™ template OT2 400 kit (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... were transiently transfected into 8 × 104 U2OS 2-6-3 cells in 4-well chamber slides using Lipofectamine 2000 reagent (Invitrogen, 11668019) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Embedded tissues were cut into 3-6 µm sections using a paraffin microtome (Leitz) and placed on SuperFrost® Plus slides (Menzel Gläser, Thermo Scientific). Deparaffination and antigen retrieval was done as described previously (Link et al. ...
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bioRxiv - Neuroscience 2020Quote: ... All 27 samples from 3 groups and 6 GIS were divided and labeled using two sets of 5 mg 11 plex TMT reagents (Thermo Scientific A34808 ...
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bioRxiv - Immunology 2022Quote: ... and the wells were washed five times with wash buffer before the addition of 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS, #37615, Thermo Fisher Scientific). Optical density was read 40 min later at 405 nm using a plate reader (SpectraMax i3 ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293t were replated every 3 days at a split ratio of 1 in 6 following dissociation with Trypsin 0.05% EDTA (Gibco, Life technologies).
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bioRxiv - Neuroscience 2021Quote: ... and immersed in reagent-2 (diluted 1:2 in PBS) for 6-24 h before incubated in reagent-2 containing TO-PRO-3 (1:5,000, Thermo Fisher Scientific) for additional 7-10 days ...
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bioRxiv - Cell Biology 2021Quote: ... The same amount of protein lysate was aliquoted into different PCR tubes and simultaneously subjected to 6 different temperatures (37, 41, 45, 49, 53, 57 °C) for 3 min in the Veriti Thermal Cycler (Applied Biosystems). After 3 min cooling on ice ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were expanded to 6 × 106/mL the day before transfection and were then diluted to 3 × 106/mL by ExpiCHO™ expression medium (Gibco). Plasmids were diluted to 20 μg/mL by cold OptiPRO medium (Gibco ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was added to cDNA and the DNA was denatured at 95°C for 3 min prior to loading in Novex 6% TBE-urea gel (Thermo Fisher). Samples were separated for 45 min at 180 V ...
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bioRxiv - Neuroscience 2022Quote: ... 3.5 cm) containing 30 larvae and a plastic-covered stirrer (magnetic stir bar micro PTFE 6 mm x 3 mm; Fisher Scientific) were placed on a magnetic stir plate (Variomag Poly 15 stirrer plate ...
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bioRxiv - Microbiology 2023Quote: ... Total DNA was stained using 4’,6-diamidino-2- phenylindole (DAPI) diluted 1:10000 in PBS containing 3% BSA (Molecular Probes) to illuminate host cell nuclei ...
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bioRxiv - Neuroscience 2023Quote: HEK-293T cells were seeded on 6-well culture plates at 3 x 106 cells/well and grown in Dulbecco modified Eagle medium (DMEM; Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 μM 6-JOE-conjugated reverse primer (5’-6-JOE-GATGATCTCCACCTTGCCGT-3’) was extended with 1 pmol of RNA as template using Superscript III (Thermo Fisher) as reverse transcriptase ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 µL of primers (final concentration 200 nM) and 6 µL of Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). The amplification conditions included an initial incubation for 2 min at 50°C ...
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bioRxiv - Cell Biology 2024Quote: ... hansenii cells were concentrated 10x in 200 μL YPD containing 40 μM FM4-64 dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) dye (Invitrogen™) to label endosomes for 8 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were subjected to 6 rounds of 10 sec sonication each at power 3 in a 550 Sonic Dismembrator (Fisher Scientific). Between each round of sonication ...
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bioRxiv - Neuroscience 2023Quote: ... differentiated OLs at 6 DIV (‘OL 3 days’) were transfected with plasmids using Lipofectamine LTX with Plus Reagent (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... and TNFα (10 ng/ml) for 6 h and stained for cleaved Caspase 3/7 green (5 µM) and propidium iodide (2 µM) (Thermo Scientific) for an additional 30 min ...
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bioRxiv - Neuroscience 2024Quote: Determination of sIL-6R was performed in the FN of NL and 21 dpi of aged WT and GFAP-IL6Tg (n=3-6/experimental group) using a precoated mouse ELISA kit (EMIL6RA, Fisher Scientific) and following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: 1.3 million HEK293T cells were seeded in a 6 cm dish in 5 ml of DMEM [high glucose DMEM (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Co-purification of 6His-tagged PGC-1α protein domains was detected by western blot using anti-6His antibody (clone His.H8, ThermoFisher Scientific MA1-21315).
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bioRxiv - Neuroscience 2024Quote: ... 20 ng/mL Interleukin-6 (IL-6) (Thermo Fisher Scientific), 20 ng/mL Interleukin-3 (IL-3 ...
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bioRxiv - Developmental Biology 2019Quote: ... pH=6 (ThermoFisher). Sections were then blocked for 1h at RT with 10% normal donkey serum (Chemicon International Inc ...
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bioRxiv - Biochemistry 2024Quote: ... or 6% (Thermofisher) tris-glycine SDS-PAGE gels then transferred onto PVDF membranes using the Transblot rapid transfer machine (BioRad) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Interleukin 6 (IL-6) was measured using an IL-6 ELISA kit following manufacturer’s instruction (Invitrogen, Catalog # CHC1263). Similarly ...
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bioRxiv - Biophysics 2021Quote: ... the soluble fraction was filtered and incubated for 3 h at 4 °C with ∼6 ml of HisPurTM Ni-NTA resin (Thermo Fisher Scientific). The beads were subsequently washed with ∼400 ml lysis buffer (without protease inhibitors or Arginine/Glutamic acid ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3 times in PBST and mounted in 4′,6-diamidino-2-phenylindole (DAPI)-containing ProLong Anti-fade Diamond mountant (Thermo Fisher Scientific).
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bioRxiv - Synthetic Biology 2020Quote: ... cells from 2-D or 3-D cultures were harvested from one well of a 6-well plate and dissociated with Accutase (Thermo Fisher #A1110501), washed three times with 1x PBS ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were washed again in PBS for 3×15 min and mounted in DAPI (4-,6-diamidino-2-phenylindole; Life Technologies, D1306) for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... total 2 μg was diluted into 6 μl of reagent (1:3 ratio) in the 100 μl of Opti-MEM I medium (31985062, Thermo Fisher Scientific) and incubated for 15 min at RT ...
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bioRxiv - Bioengineering 2022Quote: ... pools of 10 couples (n=8) were maintained up to 1 month in 6 well plates (9.6 cm2) using 3 mL of M199 (GIBCO, 41150-020, UK), DMEM (GIBCO ...
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bioRxiv - Immunology 2020Quote: ... were mixed by adding 3 μL of each (150 pmol) and 6 μL (180 pmol) of TruCut Cas9 Protein v2 (Thermo Fisher Scientific) and incubated for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 1ml of bone marrow was incubated in one well of 6-well plate with 3 ml StemPro™ MSC serum-free medium (Gibco, Thermo Scientific). Media was changed after every two days until it reached to confluency ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Following electroporation the cells were seeded across 3-wells of a 6 well plate coated with Matrigel in StemFlex supplemented with RevitaCell supplement (Thermo Fisher, A2644501). After 3 days from electroporation the cells were selected with StemFlex supplemented with G418 (25 µg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... and replated at a density of 3*106 cells/well in Matrigel-coated 6-well plates containing NB media supplemented with BDNF (Thermo Fisher PHC7074), GDNF (Thermo Fisher PHC7044) ...
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bioRxiv - Physiology 2020Quote: ... and PCR was performed on the QuantStudio 6 Flex system (3 technical replicates per mouse, Applied Biosystems, Iowa Institute of Human Genetics). Gene expression was calculated using the ΔΔct method (39 ...
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bioRxiv - Cell Biology 2021Quote: ... (Horizon Discovery Dharmacon, Lafayette, CO) in DharmaFECT 3 reagent (Horizon Discovery Dharmacon, T-2003) for 6-8 hours in OptiMEMm (Invitrogen, Waltham, MA)65 ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were rinsed with PBS (3×10min) and incubated for 6 hours with secondary donkey anti-rabbit 488 antibodies (1:1000, ThermoFisher Scientific, A11006) and streptavidin 594 (1:750 ...