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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for 6 Chloro 2H chromene 3 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The samples were subjected to 6 rounds of 10 sec sonication each at power 3 in a 550 Sonic Dismembrator (Fisher Scientific). Between each round of sonication ...
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bioRxiv - Neuroscience 2023Quote: ... differentiated OLs at 6 DIV (‘OL 3 days’) were transfected with plasmids using Lipofectamine LTX with Plus Reagent (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... and TNFα (10 ng/ml) for 6 h and stained for cleaved Caspase 3/7 green (5 µM) and propidium iodide (2 µM) (Thermo Scientific) for an additional 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 µL of primers (final concentration 200 nM) and 6 µL of Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). The amplification conditions included an initial incubation for 2 min at 50°C ...
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bioRxiv - Neuroscience 2024Quote: Determination of sIL-6R was performed in the FN of NL and 21 dpi of aged WT and GFAP-IL6Tg (n=3-6/experimental group) using a precoated mouse ELISA kit (EMIL6RA, Fisher Scientific) and following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: 1.3 million HEK293T cells were seeded in a 6 cm dish in 5 ml of DMEM [high glucose DMEM (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Cell Biology 2024Quote: ... hansenii cells were concentrated 10x in 200 μL YPD containing 40 μM FM4-64 dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) dye (Invitrogen™) to label endosomes for 8 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cell Biology 2024Quote: ... The flow-through was then dried in a speed-vac and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Neuroscience 2024Quote: ... 20 ng/mL Interleukin-6 (IL-6) (Thermo Fisher Scientific), 20 ng/mL Interleukin-3 (IL-3 ...
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bioRxiv - Cell Biology 2022Quote: Isolated colonic crypts or organoids were loaded at RT with Fura-2/AM (5 μm, 2h; Thermo Fisher Scientific) in HBS ...
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bioRxiv - Cell Biology 2021Quote: ... and RNAse A (10 μg/ml) for 2h at 50℃ in the presence of SYTOX Green (0.5 μM, Invitrogen). Flow cytometry was performed on a LSRII (BD ...
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bioRxiv - Immunology 2019Quote: ... Hybridization was performed for 2h at 40°C using the following species-specific probes: (Mouse: Cd14 (ThermoFisher, #VB1-3028230), Cd3e (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... potential was examined using 5,6-dichloro-2-[3-(5,6-dichloro-1,3-diethyl-1,3-dihydro-2H-benzimidazol-2-ylidene)-1propenyl]-1,3-diethyl-,iodide (JC-1 dye) (Life Technologies, USA) as a probe ...
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bioRxiv - Pathology 2023Quote: Fixed FDB fibers were blocked for 2h at room temperature in Superblock™ Blocking Buffer in PBS (Thermo Scientific) with 0.04% saponin ...
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bioRxiv - Molecular Biology 2023Quote: ... Gels were run at 150 V for 2h in 1× NuPAGE Tris-Acetate SDS Running Buffer (Thermo Fisher Scientific). Protein was transferred onto a PVDF membrane (Merck ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated for 2h at RT with secondary antibody mix of Alexa Fluor 647 anti-rat (Invitrogen, A-21247) and Alexa Fluor 488 anti-mouse (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... for 6h at 4°C and an additional 2h at 4°C with 20μl Dynabeads Protein G (#10003D, ThermoFisher) blocked with 0.1% BSA ...
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bioRxiv - Developmental Biology 2019Quote: ... pH=6 (ThermoFisher). Sections were then blocked for 1h at RT with 10% normal donkey serum (Chemicon International Inc ...
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bioRxiv - Biochemistry 2024Quote: ... or 6% (Thermofisher) tris-glycine SDS-PAGE gels then transferred onto PVDF membranes using the Transblot rapid transfer machine (BioRad) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Interleukin 6 (IL-6) was measured using an IL-6 ELISA kit following manufacturer’s instruction (Invitrogen, Catalog # CHC1263). Similarly ...
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bioRxiv - Biophysics 2021Quote: ... the soluble fraction was filtered and incubated for 3 h at 4 °C with ∼6 ml of HisPurTM Ni-NTA resin (Thermo Fisher Scientific). The beads were subsequently washed with ∼400 ml lysis buffer (without protease inhibitors or Arginine/Glutamic acid ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3 times in PBST and mounted in 4′,6-diamidino-2-phenylindole (DAPI)-containing ProLong Anti-fade Diamond mountant (Thermo Fisher Scientific).
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bioRxiv - Synthetic Biology 2020Quote: ... cells from 2-D or 3-D cultures were harvested from one well of a 6-well plate and dissociated with Accutase (Thermo Fisher #A1110501), washed three times with 1x PBS ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were washed again in PBS for 3×15 min and mounted in DAPI (4-,6-diamidino-2-phenylindole; Life Technologies, D1306) for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... total 2 μg was diluted into 6 μl of reagent (1:3 ratio) in the 100 μl of Opti-MEM I medium (31985062, Thermo Fisher Scientific) and incubated for 15 min at RT ...
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bioRxiv - Bioengineering 2022Quote: ... pools of 10 couples (n=8) were maintained up to 1 month in 6 well plates (9.6 cm2) using 3 mL of M199 (GIBCO, 41150-020, UK), DMEM (GIBCO ...
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bioRxiv - Immunology 2020Quote: ... were mixed by adding 3 μL of each (150 pmol) and 6 μL (180 pmol) of TruCut Cas9 Protein v2 (Thermo Fisher Scientific) and incubated for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 1ml of bone marrow was incubated in one well of 6-well plate with 3 ml StemPro™ MSC serum-free medium (Gibco, Thermo Scientific). Media was changed after every two days until it reached to confluency ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Following electroporation the cells were seeded across 3-wells of a 6 well plate coated with Matrigel in StemFlex supplemented with RevitaCell supplement (Thermo Fisher, A2644501). After 3 days from electroporation the cells were selected with StemFlex supplemented with G418 (25 µg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... and replated at a density of 3*106 cells/well in Matrigel-coated 6-well plates containing NB media supplemented with BDNF (Thermo Fisher PHC7074), GDNF (Thermo Fisher PHC7044) ...
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bioRxiv - Physiology 2020Quote: ... and PCR was performed on the QuantStudio 6 Flex system (3 technical replicates per mouse, Applied Biosystems, Iowa Institute of Human Genetics). Gene expression was calculated using the ΔΔct method (39 ...
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bioRxiv - Cell Biology 2021Quote: ... (Horizon Discovery Dharmacon, Lafayette, CO) in DharmaFECT 3 reagent (Horizon Discovery Dharmacon, T-2003) for 6-8 hours in OptiMEMm (Invitrogen, Waltham, MA)65 ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were rinsed with PBS (3×10min) and incubated for 6 hours with secondary donkey anti-rabbit 488 antibodies (1:1000, ThermoFisher Scientific, A11006) and streptavidin 594 (1:750 ...
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bioRxiv - Neuroscience 2022Quote: ... were loaded at 3 μl/min for 6 min onto a 2 cm × 75 μm C18 trap column (Acclaim Pepmap 100, 3 μm, 300 Å, Thermo Scientific) in loading buffer (0.5% v/v formic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylation of Htz1(V126C) was carried out using N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)propionamide (HPDP-Biotin) (Thermo Fisher cat. # 21341), which has a pyridyl disulfide moiety ...
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bioRxiv - Biophysics 2023Quote: ... These grids were blotted with filter paper for 3∼6 s (100% humidity at 4 °C) in a Vitrobot Mark IV (Thermo Fisher Scientific) and vitrified in liquid ethane at liquid nitrogen temperature ...
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bioRxiv - Cancer Biology 2022Quote: We purchased 6-mercaptopurine monohydrate (6-MP, #AC226520050) from Thermo Fisher.
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bioRxiv - Physiology 2023Quote: ... with or without 0.1uM 6-Hydroxyhexanoic acid (6-HHA, ThermoFisher, B24857.03).
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bioRxiv - Evolutionary Biology 2020Quote: ... Samples were then washed in PBS and incubated at room temperature for 2h in mouse anti-rabbit IgG coupled to alexa fluor 488 (green) or 594 (red; Invitrogen) at a concentration of 1:300 in 1xPBS at room temperature in the dark ...
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bioRxiv - Microbiology 2020Quote: ... and 1 mM PMSF and pre-cleared for 2h at 4°C by adding magnetic Protein G Dynabeads (Life Technologies). Beads were discarded and 1 µg of anti-CTCF antibody (Diagenode #C15410210 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in Alexa-Fluor conjugated secondary antibodies diluted in 5% normal serum in PBS for 2h at room temperature and mounted onto glass slides (Superfrost Plus) using Prolong gold (Thermofisher).
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with fluorescence conjugated secondary antibodies for 2h at RT [Alexa Fluor 488 donkey (1:1000, A21206, A21202, A11055, A21208, Invitrogen), Alexa Fluor 555 donkey (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated with the following secondary antibodies and streptavidin reagent for 2h at room temperature: goat anti-mouse IgG Alexa Fluor 488 (1:1000, ThermoFisher), goat anti-rabbit CF568 (1:1000 ...
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bioRxiv - Bioengineering 2021Quote: ... samples were cut into pieces of 4mm2 and incubated 2h at 37°C in dispase 1X (Invitrogen, Cergy Pontoise France). Epidermis and dermis were dissociated with forceps ...
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bioRxiv - Neuroscience 2021Quote: Sections were then washed with PBS and incubated for 2h at 22-24 °C with Alexa Fluor 488– conjugated secondary antibody (cat. no A32731, Invitrogen), which was added at a dilution of 1:500 in the same solution as the primary antibody.
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bioRxiv - Neuroscience 2020Quote: ... and 2mM PMSF) and heated to 98°C for 30 min and 90°C for 2h on a heat block (ThermoFisher). Samples were mixed well and centrifuged at 22,000 × g for 30 min at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were washed the following morning and incubated for a minimum of 2h at RT with secondary antibody (rabbit Alexa Fluor-488 and rabbit Alexa Fluor-647, ThermoFisher). Finally ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were washed the following morning and incubated for a minimum of 2h at RT with secondary antibody (1:1000, ant-rabbit Alexa Fluor-647, Invitrogen). Finally ...
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bioRxiv - Bioengineering 2022Quote: ... was incubated for 2h at room temperature at 1:500 together with alexa-coupled wheat germ agglutinin (WGA, 1:500, Thermofisher). Sections were mounted in DAPI Fluoromount-G (SouthernBiotech ...