Labshake search
Citations for Thermo Fisher :
101 - 150 of 10000+ citations for 6 Chloro 2H chromene 3 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2019Quote: ... and incubated with secondary antibodies (2h at room temperature, goat IgG A647, Molecular Probes/Invitrogen) and DAPI ...
-
bioRxiv - Neuroscience 2019Quote: ... and incubated with secondary antibodies (2h at room temperature, goat IgG A647, Molecular Probes/Invitrogen) and DAPI ...
-
bioRxiv - Genomics 2019Quote: ... the islets were hand-picked and incubated for 2h in RPMI 1640 GlutaMAX medium (Invitrogen) containing 11 mM glucose and 2 mM L-glutamine and supplemented with 10% fetal calf serum (Gibco) ...
-
bioRxiv - Developmental Biology 2020Quote: ... and precipitated after a 2h long incubation with magnetic beads (Dynabeads Protein A, Invitrogen 10001D). Washes were carried out in RIPA-LS ...
-
bioRxiv - Neuroscience 2021Quote: ... 2h) conjugated to AF-568 (1:500 goat-anti-rabbit, Life Technologies #A11011; RRID: AB_143157) or AF-647 (1:500 goat-anti-mouse ...
-
bioRxiv - Developmental Biology 2020Quote: ... After blocking for 2h at RT in CAS-Block (1:10 in PBST; ThermoFisher, #008120) blocking reagent was replaced by antibody solution (diluted in CAS-Block ...
-
bioRxiv - Plant Biology 2020Quote: ... on 4-16% (Figures 4A and 7) or 3-12% (Figures 4C and 6) NativePAGE gels (Life technologies). Cathode Running buffer (Life technologies ...
-
bioRxiv - Biophysics 2019Quote: ... 4-(2-(6-(dibutylamino)-2-naphthalenyl)ethenyl)-1-(3-sulfopropyl)-,hydroxide (di-4-ANEPPS) was purchased from Invitrogen. It was dissolved in ethanol and added to the dried lipid film at a 12:1 lipid:probe molar ratio ...
-
bioRxiv - Biochemistry 2021Quote: ... and then diluted into fresh YPD for 3-6 hours before inoculating into 96-well plates (Thermo Scientific) at a starting OD600 between 0.04 to 0.1 ...
-
BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Reverse 5′-CGA AGG TGT GAC TTC CAT G-3′) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
-
bioRxiv - Biochemistry 2021Quote: ... and then diluted into fresh YPD for 3-6 hours before inoculating into 96-well plates (Thermo Scientific) at a starting OD600 between 0.04 to 0.1 ...
-
bioRxiv - Neuroscience 2022Quote: ... the cerebral cortexes from 2-3 P3-P5 C57BL/6 mice were collected in ice cold HBSS (Invitrogen), the tissue was washed three times with HBSS and digested with 0.04% trypsin (Sigma ...
-
bioRxiv - Cell Biology 2022Quote: HCT116 cells were cultured in a 6-well plate at 300,000 cells per well in 3 ml McCoy’s 5A medium (Gibco) supplemented with 10% heat inactivated FBS and with 90 UI/mL Penicillin ...
-
bioRxiv - Neuroscience 2020Quote: Neocortex from C57Bl/6 pups (postnatal day 1-3) was dissected in Hibernate-A medium (#A1247501, Gibco, USA), digested with papain (#LS003124 ...
-
bioRxiv - Cell Biology 2022Quote: ... For the bulk endocytosis experiments using the 4-[6-[4-(diethylamino)phenyl]-1,3,5-hexatrien-1-yl]-1-[3-(triethylammonio)propyl]-pyridiniumbromide dye (FM 4-64, Invitrogen), the cells were prepared as previously described [41] ...
-
bioRxiv - Cell Biology 2023Quote: ... X-biotin-PE [N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine] was obtained from Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2023Quote: ... All nuclei were pooled and stained with DAPI (4′,6-diamidino-2-phenylindole, 3 uM final) (Invitrogen, D1306). Using a FACSAria III cell sorter (BD Biosciences) ...
-
bioRxiv - Biophysics 2023Quote: ... in 3% BSA with a 1:50 ratio and 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) were employed ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were marked with 2,3x10-3 µg/µL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 min at RT in the dark ...
-
bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 30 ovaries from 3-6 day-old flies using TRIzol (Thermo Fisher Scientific) in three replicates ...
-
bioRxiv - Cancer Biology 2019Quote: ... overnight at 4°C followed by appropriate secondary antibody incubation for 1-2h (Thermo Fisher Scientific). IF samples were imaged using Zeiss LSM 710 confocal microscope ...
-
bioRxiv - Cancer Biology 2019Quote: ... and digested for 2h at 37 at 110rpm in RPMI-1640 (Thermo Fisher, with L-glutamine) containing antibiotic-antimycotic solution ...
-
bioRxiv - Physiology 2020Quote: ... interleukin 6 (IL-6; Invitrogen, Carlsbad, CA), plasminogen activator inhibitor 1 (PAI-1 ...
-
bioRxiv - Biochemistry 2020Quote: ... HEK 293 cells were plated onto 6-well plates (2.5-4.5 × 105 cells per well) in 3 ml of MEM (Life Technologies) with 10% FBS (Life Technologies ...
-
bioRxiv - Cell Biology 2021Quote: ... embryos at the 3-6 somite stage were embedded oriented laterally in 1% low-melt agarose (Invitrogen, Carlsbad, CA) and imaged under bright field (to determine yolk elongation by taking major and minor axis measurements ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with lentiviral supernatant and 3 days later selection begun with 6 μg/mL puromycin (Thermo Fisher), which was maintained during all subsequent culturing.
-
bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...
-
bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...
-
bioRxiv - Molecular Biology 2020Quote: ... flushed with argon prior to adding hydrochloric acid (3 mL of 6 M sequencing grade solution; Thermo Scientific #PI24308). Sealed tubes were kept at 125° C for 48h (oil bath ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were marked with 2,3×10−3 μg/μL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 minutes at room temperature in the dark ...
-
bioRxiv - Developmental Biology 2022Quote: ... and passaged very 3-4 days with a 1:4-6 passage ratio using Versene solution (Life Technologies 15040066).
-
bioRxiv - Neuroscience 2019Quote: ... harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number, NM_000289.1) using Lipofectamine LTX-PLUS Reagent (Life Technologies) according with manufacturer’s protocol ...
-
bioRxiv - Physiology 2019Quote: ... Each 6 μL assay master mix (AMM) consisted of 3 μL of TaqMan® Gene Expression assay 20X (ThermoFisher) and 3 μL of 2X Assay Loading Reagent (Fluidigm) ...
-
bioRxiv - Evolutionary Biology 2020Quote: 3-6-day old female ovaries were dissected from each experimental genotype and placed directly in Trizol reagent (Invitrogen), and homogenized ...
-
bioRxiv - Cell Biology 2023Quote: ... No mycoplasma were detected in cultures by 4′,6-diamidino-2-phenylindole or TO-PRO-3 (Thermo Fisher Scientific) staining.
-
bioRxiv - Microbiology 2023Quote: ... coverslips were washed once with 0.1M MOPS buffer (pH 3) and stained with 50 μg / ml 5,(6)-Carboxyfluorescein Diacetate (CFDA) (Invitrogen) in MOPS buffer for 1 h at 37°C in the dark ...
-
bioRxiv - Neuroscience 2023Quote: ... The parental hiPS cell line (8858-3) was maintained in 6-well plates using StemFlex medium (Life Technologies, A3349401). Cas9 ...
-
bioRxiv - Pathology 2024Quote: ... Cells were subcultured at a 1:3 to 1:6 ratio using Gibco TrypLETM Express Enzyme (12605010, Fisher Scientific) for cell dissociation ...
-
bioRxiv - Neuroscience 2024Quote: ... n=6) before being perfused intravenously with fluorescent Texas Red 40 kDa Dextran (3 mg/kg; D1829, Fisher Scientific) as described in previous studies (25,26 ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by 2h with a first secondary antibody (rabbit Alexa Fluor-488–conjugated, 1:500, Invitrogen, Canada). A blocking step of antigenic sites from the first primary and secondary antibody combination was performed thereafter by a 3h incubation with normal serum from the same species as the primary antibody ...
-
bioRxiv - Molecular Biology 2020Quote: ... stained for 2h at RT with secondary antibody Donkey Anti-Rabbit IgG – Alexa Fluor 555 (Life Technologies) 1/500 in PBST ...
-
bioRxiv - Immunology 2022Quote: ... conjugated to horseradish peroxidase (HRP) were incubated 2h at room temperature and detected using Pierce ECL (Thermofisher) and signal was quantified by ChemiDoc™ Touch Gel Imaging System (BIO RAD).
-
bioRxiv - Neuroscience 2020Quote: ... followed by 2h incubation in secondary antibody (goat anti-mouse Alexa 647, 1:200, Invitrogen A-21236). Slices were then mounted and scanned on a Leica TCS SP8 STED 3X confocal with white light laser and imaged with a 40X/1,3 NA oil immersion objective ...
-
bioRxiv - Plant Biology 2020Quote: ... slides were incubated 2h with a goat anti-mouse antibody coupled to Alexa fluor 568 (Invitrogen, A11004) at 1:1000 in 3% BSA ...
-
bioRxiv - Cell Biology 2022Quote: ... they were concentrated from 40 μl to 5 μl during 2h at low temperature using speedvac (Thermofisher). Small RNA libraries were then built with Illumina TruSeq Small RNA Sample kit following manufacturer’s recommendations ...
-
bioRxiv - Biophysics 2023Quote: Cryo-EM data of BmrCD_IF-2H/ATP were collected by Titan Krios G4 microscope (Thermo Fisher Scientific) at 300 keV ...
-
bioRxiv - Biochemistry 2023Quote: ... Co-IP samples were incubated for 2h with 1.5mg PBST-washed Protein G Dynabeads® (Life Technologies) at 4°C before centrifugation and bead removal using a DynaMag™-2 Magnet (Invitrogen) ...
-
bioRxiv - Cell Biology 2024Quote: ... then incubated for 2h at room temp with Alexa Fluor-conjugated secondary antibodies (1:400; Molecular Probes) and counterstained with phalloidin for F-actin (Invitrogen ...
-
bioRxiv - Neuroscience 2024Quote: ... cells were washed three times and incubated for 2h with secondary antibodies AlexaFluor488 or AlexaFluor647 (Life Technologies) and chromatin stained with DAPI (1μg/mL ...
-
bioRxiv - Immunology 2021Quote: ... mouse interleukin 6 (IL-6; ThermoFisher; 1:500). Sections were then stained for DAPI (Sigma ...