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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for 5 M TOLYL FURAN 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The plate was incubated for approximately 5 min before the reaction was stopped with the addition of 2 M H2SO4 and the A450 was measured (Multiskan FC, ThermoFisher). A standard curve was established using serial dilutions of lysate from PA11 ...
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bioRxiv - Biochemistry 2022Quote: ... Eluates were diluted to 2 M urea in 50 mM ammonium bicarbonate and digested with 1 μg of trypsin (ThermoFisher) overnight at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysates were incubated for 2 h at 4°C with IgG-coupled Dynabeads (Dynabeads M-270 Epoxy; Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... 4 µM Cas9 protein (IDT) and 2 µM of each annealed cr/tracr RNA were combined in Opti-MEM media (Gibco 11058-021 ...
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bioRxiv - Neuroscience 2023Quote: ... and the nuclei pellet was resuspended in 2 mL of ice-cold resuspension buffer (1 M HEPES [#J16924- AE, ThermoFisher Scientific] ...
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bioRxiv - Biophysics 2023Quote: ... and 2 M NaCl) were incubated in a dialysis chamber (Slide-A-Lyzer MINI Dialysis Devices, 3.5K MWCO, Thermo Scientific) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The reactions were quenched with 2 μl of 2.7 M ammonium bicarbonate before loading on Bolt™ 4–12% Bis-Tris Plus gels (Invitrogen) for separation ...
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bioRxiv - Cancer Biology 2023Quote: ... 4 times with 6 m/s and 2 times with 6.5 m/s intermitted by cooling on ice using MP Biomedicals™ Lysematrix D (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... iPSCs were transfected with PB-TO-hNGN2 vector (gift from M. Ward, NIH, Maryland) in a 1:2 ratio (transposase:vector) using Lipofectamine Stem (Thermo Fisher). After 72 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Purifications were carried out from 150 μl of the whole-cell extract (2 mg protein) per experiment using 37 µl of Dynabeads M-280 sheep anti-rabbit IgG (Invitrogen) and 2 µl of the anti-LytA antibody (Biomedal) ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed (3x for 5 minutes at room temperature while shaking) with 0.1 M PBS and 0.1% Triton-X-100 (Acros Organics, AC21568-2500), then nutated in blocking solution containing normal goat serum (Millipore Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were washed five times with lysis buffer containing 1 M NaCl and incubated with 25 µl of biotin elution buffer (lysis buffer with 5 mM D-biotin [Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were sampled on a 300 μm x 5 mm PepMap C18 precolumn and separated on a 75 μm x 250 mm C18 column (PepMap, Thermo Scientific) using a 120-min gradient ...
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bioRxiv - Immunology 2022Quote: 90μL of paraformaldehyde fixed cells was incubated with 5 x 10-7 M DAPI and 1:100 Vybrant™ DiD Cell-Labeling Solution (Invitrogen) at 37C for 20 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... Serial dilutions of gene fragments were performed in sheared salmon sperm DNA at 5 µg mL−1 (m/v) (Thermofisher, LT) diluted in Tris-EDTA (TE ...
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bioRxiv - Cancer Biology 2022Quote: Aliquots (125 μL) of sEV- or m/lEV-fractions in PBS were stained with 5 μM CellTrace Violet (CTV; C34571, Thermo Fisher) for 10 min at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... and 100 ng of purified DNA was subsequently incubated with 0.2 M dATP and 5 U DreamTaq Polymerase (Thermo Fisher Scientific) in DreamTaq buffer (10 μL total volume ...
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bioRxiv - Molecular Biology 2023Quote: ... Serial 10-fold dilutions of gene fragments were performed in sheared salmon sperm DNA 5 µg mL-1 (m/v) (Thermofisher, LT) diluted in Tris-EDTA (TE ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were pelleted by centrifugation at 1,000 g for 5 min and lysed with M-PER™ mammalian protein extraction reagent (Thermo Scientific) supplemented with Halt™ protease and phosphatase inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed with DPBS and were pelleted by centrifugation at 1,000 g for 5 min and lysed with M-PER™ mammalian protein extraction reagent (Thermo Scientific) supplemented with the Halt™ protease and phosphatase inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed for 5 minutes in 0.1 M Tris and mounted with coverglass in ProLong gold with DAPI (Invitrogen, Cat#P36931). Fluorescent slides were imaged at 20x magnification on a Zeiss AxioScan 7 microscope.
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bioRxiv - Microbiology 2023Quote: ... 5% CO2 for 24 h before the cells were lysed with 300 µl M-PER Mammalian Protein Extraction Reagent (Thermo Scientific). A volume of 10 µl was resolved on a 3-8% Tris Acetate gel ...
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bioRxiv - Molecular Biology 2024Quote: ... the supernatant was collected and 200 µg were incubated with 5 μl of buffer equilibrated DynabeadsTM M-280 Streptavidin (Invitrogen, 11205D) for 15 min at 4 °C in rotation to perform a pre-clearing step ...
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bioRxiv - Biochemistry 2024Quote: ... 0.15 pmol DNA of 3 kb PCR substrates with a single biotin (GC227 and GC228) were coupled with 5 μl of Dynabeads M-280 streptavidin (Invitrogen 11205D) in 10 μl of binding buffer (5 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... The βarr1/2 siRNA (5’-ACCUGCGCCUUCCGCUAUG-3’) and a scrambled siRNA (control, 5’-UGGUUUACAUGUCGACUAA-3’) (Dharmacon) were transfected by RNAimax (Invitrogen) according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... After the fourth EDTA incubation the pieces were cut into 2 mm2 pieces and placed in 5 mL digestion solution containing 5% fetal bovine serum (Gibco), 10 mM HEPES (Gibco) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples (5 μl) were injected on a C18 PepMap trap column (5 μm, 100 μm I.D. x 2 cm, Thermo Scientific) at 10 μl/min ...
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bioRxiv - Biochemistry 2022Quote: ... Samples (5 μl) were injected on a C18 PepMap trap column (5 μm, 100 μm I.D. x 2 cm, Thermo Scientific) at 20 μl/min ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated for 2-24hrs at 37°C 5% CO2 +/- myristoylated aPKC pseudosubstrate inhibitor (5 μM; Invitrogen; Product number 77749) in IMDM+0.1% BSA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... supplemented with 15% FBS and 10% Gibco Phytohemagglutinin M form (PHA-M) (Thermo Fisher, #10576015) stimulated leukocyte conditioned medium ...
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bioRxiv - Plant Biology 2023Quote: ... 6 uL of 0.5 M EGTA (bioWORLD) and 6 uL of 0.5 M EDTA (Invitrogen) were added to quench the reaction at 65 °C for 10min ...
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bioRxiv - Bioengineering 2023Quote: ... The concentrated virus was diluted 1:5 in hepatocyte medium containing N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (HEPES; 20 mM; Gibco) and 4 μm/mL polybrene (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... with IVT mix (0.5 µL Ribolock inhibitor, Invitrogen, 2 µL T7 polymerase buffer, NEB, 8 µL rNTP mix, 2 µL T7 polymerase, Invitrogen). DNA was removed by addition of 1 µL turbo DNase (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: Mice were injected with 100 μg of 5-ethynyl-2’deoxyuridine (EdU, Invitrogen) 2 hours prior to splenocyte harvest ...
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bioRxiv - Developmental Biology 2021Quote: ... with 50-100 nl of 1 mM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen) at stage 41 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were counterstained with 2 μM CellTracker Green (5-chloromethylfluorescein diacetate dye) (ThermoFisher) followed by NucBlue (ThermoFisher ...
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bioRxiv - Biophysics 2022Quote: ... NPE-caged ATP (adenosine 5’-triphosphate, P3- (1-(2-nitrophenyl ethyl) ester) (Invitrogen) dissolved in attachment buffer was photolyzed in the AFM chamber using an UV light source at 340 nm ...
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bioRxiv - Cell Biology 2022Quote: ... (B-5-1-2 coupled to Alexa-488, Thermo Fisher or DM1A, Sigma), rat anti-tyrosinated tubulin (YL1/2 ...
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bioRxiv - Microbiology 2020Quote: ... 5 mM tris(2-carboxyethyl)phosphine (TCEP, Bond-Breaker, neutral pH, Thermo Scientific) was added and samples were incubated at room temperature for 20 min ...
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bioRxiv - Immunology 2019Quote: ... cells were loaded with 5 mM Fura-2-AM/neurobasal media (Thermo Fisher) at 37°C for 30 min ...
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bioRxiv - Developmental Biology 2019Quote: To detect cellular proliferation Click-it EdU (5-ethynyl-2’-deoxyuridine) kits (Invitrogen # C10337 or # C10339 ...
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bioRxiv - Microbiology 2021Quote: ... mice were injected with 100 μg of 5-ethynyl-2’deoxyuridine (EdU, Invitrogen) 3.5 hours prior to splenocyte harvest ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl) phosphine hydrochloride (Thermo Fisher) for 30 min and alkylated with 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 mM succinimidyl 3-(2-pyridyldithio)propionate (SPDP) (Thermo Fisher Scientific, USA) in PBS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... monensin (5 μM) and retro-2 (100 μM) were obtained from Molecular Probes [B7450] ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were blocked for 2 hr with 5% goat serum (Thermo Fisher Scientific) and 5% bovine serum albumin (BSA ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Proteins were reduced with 5 mM Tris (2-carboxyethyl) phosphine hydrochloride (Thermo Fisher) for 30 min and alkylated with 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-(and-6)-chloromethyl-2′,7′ dicholorodihydrofluorescein diacetate (CM-H2DCFDA; Molecular Probes C6827), was used to visualize ROS accumulation (excitation ...
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bioRxiv - Immunology 2022Quote: ... or 2.5μM of 5-(and-6)-Carboxy-2’,7’-Dichlorofluorescein Diacetate (DCFDA) (Invitrogen) was then added and incubated with the cells 20 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... followed by blocking for 2 hours in 5% goat serum (Invitrogen, Waltham, MA) in PBS ...