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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for 5 M TOLYL FURAN 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... passed through Xylol (2 x 5 minutes) and immediately mounted with Eukitt® (ThermoFisher).
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bioRxiv - Developmental Biology 2020Quote: ... viride were incubated in 100 μM 5-ethynyl-2’-deoxyuridine (EdU; Thermo Fisher Scientific) diluted in ASW from 24 to 48 HPA ...
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bioRxiv - Microbiology 2021Quote: ... All medium were supplemented with 2-5 or 10% fetal bovine serum (FBS; Gibco) according to the experimental setting ...
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bioRxiv - Neuroscience 2021Quote: ... and 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride (INT, #I00671G, Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... a ClickiT ® EdU (5-ethynl-2’-deoxyuridine) cell proliferation assay (Invitrogen, Paisley, UK) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated with 5-ehtynyl-2’-deox-yuridine (EdU: Invitrogen, Thermo Fisher Scientific) at 10 µM ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated with 5-ehtynyl-2’-deox-yuridine (EdU: Invitrogen, Thermo Fisher Scientific) at 10 µM ...
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bioRxiv - Molecular Biology 2021Quote: ... 2×10^5 individualized hESCs were resuspended in 10 μL Resuspension Buffer R (Invitrogen) containing CRISPR ribonucleoproteins (Cas9 protein+sgRNA ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM DTT and 5 U/mL SUPERase.In RNase Inhibitor(Thermo Fisher Scientific #AM2696)) on ice and post-nuclear extracts placed on top of 10-50% weight/volume sucrose gradients containing the same buffer (apart from no Triton X-100 ...
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bioRxiv - Cancer Biology 2021Quote: ... The target cells were treated with 5 mg/ml MMC (#BP2531-2, Fisher Scientific) for 90 minutes at 37°C and then washed twice with RPMI medium before adding to the T cells at a 2:1 ratio (CAR T:MMC treated cells) ...
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bioRxiv - Developmental Biology 2021Quote: Fish were incubated with 0.1 mg/ml of 5-ethynl-2-deoxyuridine (Thermo Fisher) in embryo media for 2 hours prior to harvesting at 98 hpf ...
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bioRxiv - Cancer Biology 2022Quote: ... mice underwent intraperitoneal injection (IP) with 5-ethynyl-2`-deoxyuridine (EdU) (component A, Invitrogen™ Click-iT™ EdU Cell Proliferation Kit for Imaging ...
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bioRxiv - Developmental Biology 2022Quote: ... Nuclei were stained with 5 µg / µl 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) and slides were mounted with FluoromountG (SouthernBiotech).
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bioRxiv - Molecular Biology 2023Quote: ... 2 hours and 5 hours using a Floid Cell Imaging Station (Thermo Fisher Scientist), and then manually counted for quantification ...
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bioRxiv - Evolutionary Biology 2023Quote: We performed experimental evolution using SM/5 media [56] (2 g glucose (Fisher Scientific), 2 g BactoPeptone (Oxoid) ...
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bioRxiv - Cell Biology 2023Quote: ... Cy-2- or Cy-5-conjugated goat anti-mouse secondary antibodies (Thermo Fisher Scientific) were used at a 1:750 dilution in 5% HIGS/PBS ...
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bioRxiv - Biophysics 2023Quote: ... 2’(3’)-O-(2,4,6-trinitrophenyl)-adenosine 5’-triphosphate (TNP-ATP) was purchased from Invitrogen as a trisodium salt and stored in the dark at -20 °C at 10 mg/ml_ ...
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bioRxiv - Genetics 2023Quote: ... 2×10^5 individualized iPSCs were resuspended in 10 μL Resuspension Buffer R (Invitrogen) containing CRISPR ribonucleoproteins (Cas9 nuclease +sgRNA ...
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bioRxiv - Neuroscience 2023Quote: ... then they were incubated for 2 h in 5% normal donkey serum (NDS, Gibco), 0.2% Triton-X100 in PBS to block nonspecific binding ...
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bioRxiv - Developmental Biology 2023Quote: Cell proliferation assays were conducted employing 5-Ethynyl-2′-deoxyuridine (EdU; ThermoFisher Cat# C10337), a thymidine analog ...
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bioRxiv - Biochemistry 2021Quote: ... pH standard buffers (pH ~6.5 and ~7.5 (0.025 M HEPES, 0.105 M KCl, 0.001 MgCl2)) were prepared with 10 μM nigericin (Invitrogen, N1495) (a protonophore ...
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bioRxiv - Biochemistry 2021Quote: ... pH standard buffers (pH ~6.5 and ~7.5 (0.025 M HEPES, 0.105 M KCl, 0.001 MgCl2)) were prepared with 10 μM nigericin (Invitrogen, N1495) and added to cells to equilibrate pHe and pHi as previously described.28 Individual cell pHi was back-calculated using single-cell standard curves and reported in Figure S1C.
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bioRxiv - Bioengineering 2023Quote: ... Dimpled microparticles were obtained after FA release in phosphate buffered saline (PBS; 0.1 M, pH 7.4, 0.15 M NaCl, Gibco) at 37°C for 7 days.
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bioRxiv - Cell Biology 2021Quote: ... cells were pulsed with 10µM 5-Ethynyl-2’deoxyuridine (EdU) for 2 hours following manufacturer’s instructions (Click-iT® EdU Alexa Fluor® Imaging Kit, Life Technologies). Cells were subsequently fixed and stained with Hoechst 33342 ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Neuroscience 2022Quote: ... and 12 old (21 months) male C57BL/6 animals (combined over 2 independent experiments) were intraperitoneally injected with 5-ethynyl-2’- deoxyuridine (EdU) (Fisher Scientific, A10044) (resuspended in PBS at 5 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were loaded onto a 2 cm pre-column (C18, 5 μm, 100 A°, 100 μ, 2 cm Nano-viper column # 164564, Thermo Scientific) at 5 μl/minute flow rate using loading pump for about 5 minutes and then resolved the peptides on a 50 cm analytical column (C18 ...
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bioRxiv - Cell Biology 2022Quote: ... 2’,7’-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Cell Biology 2023Quote: ... Treatments were incubated 2 hours before addition of the MTS (3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) reagent (Thermo-Fisher Scientific, Waltham, MA) and incubation was continued another 1.5 hours ...
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bioRxiv - Biophysics 2024Quote: ... Cell detachment for cryopreservation or experiments was achieved through 30 minutes of incubation with 5 mM EDTA in PBS without Ca+2 and Mg+2 (PBS-/-) followed by TrypLE Express dissociation agent (Gibco, 12605-010) for five minutes at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... x 5 mm 5 μm 100 Å and an Acclaim PepMap RSLC 75 μm x 50 cm 2 μm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Dionex) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µL of 5 mM aa-dUTP/dNTP mix (5 mM each dATP, dGTP and dCTP, 3 mM dTTP and 2 mM 5-(3-aminoallyl)-dUTP (Thermo Fisher Scientific, AM8439)) and 1.25 µL of 40 µM oligo 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated in 2 μM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, cat no: T3168) at 37°C for 15 min ...
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bioRxiv - Developmental Biology 2021Quote: Preparation of lipid/cholesterol depleted fetal bovine serum (FBS): 500mL FBS were stirred overnight at 4°C with 10g Cab-osil M-5 (ACROS Organics 7631-86-9). The mix was then centrifuged for 10min at 3000rpm and the supernatant filtrated under sterile conditions ...
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bioRxiv - Biochemistry 2020Quote: ... with a flow rate of 5 μ l/min and separated on an EASY-Spray HPLC Column (75 μ m × 150 mm, Thermo Fisher Scientific). HPLC gradient was conducted using 5-55% of the mobile phase (75% acetonitrile and 0.1% formic acid ...
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bioRxiv - Microbiology 2023Quote: ... and 8 mM MgSO4) for 1.5 minutes at maximum speed (5 m/s) using a Fisherbrand™ Bead Mill 4 Homogenizer (Fisher Scientific, Waltham, MA, USA). Tissue homogenates were centrifuged at 6,000 g for 6 minutes at room temperature and syringe-filtered through 0.45-μm Sterivex filters (Millipore ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized from 2 µg total RNA by reverse transcription using random primers with M-MLV reverse transcriptase (Thermo Fisher Scientific, 28025013). Real-time PCR was performed on QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... 5 μm, 100 Å) precolumn and separated on an Acclaim PepMap RSLC 75 μm × 50 cm (C18, 2 μm, 100 Å) column (Thermo Scientific). The flow rate was 220 nL·min−1 with a total analysis time of 90 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... individual blastomeres were washed thoroughly in Research Wash Medium and either subjected to imaging using the hyperspectral microscope or reaggregated in 2% phytohaemagglutinin M (ThermoFisher Scientific, Waltham, Massachusetts) in Research Cleave Medium to generate chimeric blastocysts ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins on the beads were eluted with 300 μl DLB containing 2 mM biotin and precipitated by adding 1/10 volume of 8.74 M Trichloroacetic acid solution (TCA, Fisher Scientific, Cat# BP555-500). Precipitated proteins were washed once with 500 μl of 0.874 M TCA and four times with ice-cold acetone ...
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bioRxiv - Biophysics 2021Quote: ... 1 μM AF568-labeled actin without or with 2 μM C0-C2 in M-ABB was dispensed by a Multidrop Combi Reagent Dispenser (ThermoFisher Scientific, Waltham, MA) into the 1,536-well assay plates containing the compounds ...
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bioRxiv - Neuroscience 2023Quote: ... An Acclaim PepMap (75μm x 2 cm) trap column together with a EasySpray ES803 column (75 µm x 50 mm, 100 Ȧ) (both Thermo Fisher Scientific) were employed for nanoLC with 3 µL peptide extract being injected.
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bioRxiv - Biophysics 2020Quote: ... The concentrations of cdM2-1 and RNA were determined by absorbance at 280 and 260 nm using extinction coefficients of 5,960 M−1·cm−1 (Expasy-ProtParam) (14) and 8,050 M−1·cm−1·base−1 (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... Dynabeads M-280 Protein G (ThermoFisher, 10003D) were resuspended by vortexing for 30 s ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 µl Streptavidin M-280 Dynabeads (Invitrogen). α-H3K27me3 ChIP - 0.8 µg chromatin ...
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bioRxiv - Developmental Biology 2021Quote: ... 4 μL 0.1 M DTT (Thermo Fisher) and 2 μL SuperScript III Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... -M and -NS) using Lipofectamine 2000 (Invitrogen). At 12 h p.t. ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μL of 0.1 M DTT (Invitrogen), 1 μL murine RNase inhibitor (NEB) ...
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bioRxiv - Genomics 2021Quote: ... 0.23 μL magnesium chloride (1 M, Ambion), 0.48 μL Recombinant RNAse Inhibitor (40 U/μL ...
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bioRxiv - Genomics 2021Quote: ... solution including 0.002 M EDTA (Thermo Scientific), 1% BSA (Serva ...