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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... and measured on an ABI PRISM 7500 or ABI QuantStudio 3 sequence detection system (Applied Biosystems). Results were quantified using the 2-ΔΔCt method and shown as arbitrary units relative to control groups ...
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bioRxiv - Physiology 2020Quote: ... and measured on an ABI PRISM 7500 or ABI QuantStudio 3 sequence detection system (Applied Biosystems). Results were quantified using the 2-ΔΔCt method and shown as arbitrary units relative to control groups ...
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bioRxiv - Microbiology 2022Quote: Quantitative real time PCR was performed using a QuantStudio 3 real-time detection system (Applied Biosystems). Fast SYBR Green master mix (Applied Biosystems / Thermo Fisher ...
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bioRxiv - Developmental Biology 2024Quote: ... follicles were incubated with 500 nM CellEvent™ Caspase-3/7 Green Detection Reagent (C10427, Invitrogen). Oocytes were stained with 150 nM Sir-Tubulin (CY-SC002 ...
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bioRxiv - Molecular Biology 2021Quote: 5-ethynyl-2’-deoxyuridine (EdU)-incorporation assay has been performed using a commercially available kit (Invitrogen, C10340). Animals were injected intraperitoneally with 60 µl of EdU (0.5 mg/ml in 50% DMSO and 50% PBS solution ...
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bioRxiv - Neuroscience 2019Quote: ... incubation for 3-5’ in 3,3’ diaminobenzidine (DAB, Acros Organics) staining solution (0.025% w/v DAB ...
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bioRxiv - Neuroscience 2020Quote: ... 5’-GCCTCCCATCCACAAGAATAGTG-3’ and cloned into pCRII-Blunt-TOPO (Invitrogen). This plasmid was then used to generate digoxigenin-labeled sense and antisense probes.
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bioRxiv - Immunology 2021Quote: ... ENV probe (5’-/VIC/CCTTGGGTTCTTGGGA-3’/MGB, Thermo Fisher Scientific), Gag forward (5’-ATGTTTTCAGCATTATCAGAAGGA-3’) ...
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bioRxiv - Immunology 2021Quote: ... Pol probe (5’-/NED/AAGCCAGGAATGGATGGCC-3’/MGB, Thermo Fisher Scientific). Thermostabe DNA polymerase was made in-house by transforming E ...
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bioRxiv - Molecular Biology 2020Quote: ... a control scrambled RNA (customed, Ambion, sense: 5’-UUCUCCGAACGUGUCACGUtt-3’) was used ...
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bioRxiv - Cell Biology 2020Quote: ... 3-5 minute incubation with Tryple Express Trypsin (Thermo Fisher), and dilution and gentle trituration in complete media ...
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bioRxiv - Immunology 2021Quote: ... tetramethylindocarbocyanine perchlorate;CILC18(3) (5 µM/mL DiI, ThermoFisher-Invitrogen) and adoptively transferred intravenously into non-myeloablated Lyve1-GFP+ mice ...
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bioRxiv - Immunology 2021Quote: ... tetramethylindocarbocyanine perchlorate;CILC18(3) (5 µM/mL DiI, ThermoFisher-Invitrogen) and adoptively transferred intravenously into non-myeloablated Lyve1-GFP+ mice ...
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bioRxiv - Cell Biology 2020Quote: ... TPD53: 5’-GUCUCCAGCAAUAGGAUGAUUUACUA-3’) with Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and removed by treatment with 3-5 mL trypsin (Gibco) then pelleted by centrifugation at 300 × g for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... 5’- UUUCCUUCCACUCGGAUAAGAUGCUGA-3’ were transfected with RNAiMaX (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Vero-CCL81 [for MuV and VSV_RUBV] or Vero-hSLAM [for MeV-IC323] target cells which were seeded at a density of 2 × 104 cells per well in flat-bottom 96-well plates (Fisher Scientific, #08-772-3). The cells were incubated at 37 °C/5% CO2 overnight (∼20 hours) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Cancer Biology 2019Quote: ... White MicroLiteTM 2+ 96-well plates (Thermo Scientific, #7572) were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 µg/well of purified BSA (NEB ...
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bioRxiv - Immunology 2021Quote: ... 96-well EU Immulon 2 HB plates (Thermo Scientific) were coated overnight at 4°C with the SARS-CoV-2 S1 subunit (amino acids 1–685 ...
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bioRxiv - Molecular Biology 2022Quote: ... YES plates were made by adding 2 % agar (Gibco) to YES media ...
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bioRxiv - Developmental Biology 2020Quote: mESC lines were cultured in 2-inhibitors based medium (Advanced DMEM/F12:Neurobasal (1:1) medium (Gibco), supplemented with 2.5% ESC-grade fetal bovine serum (vol/vol ...
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bioRxiv - Cancer Biology 2023Quote: ... Emulsion polymerase chain reaction (PCR) based target enrichment was performed using Ion OneTouch 2 system (ThermoFisher Scientific). Enriched libraries were sequenced using an Ion Torrent Personal Genome Machine (PGM ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was replaced every 2-3 days and cells passaged 1:2 or 1:3 weekly with 0.25% Trypsin/EDTA (Thermo Fisher Scientific, #25200-056) pre-warmed at 37°C.
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bioRxiv - Biochemistry 2021Quote: ... Detection of relative luciferase units was carried out using a Varioskan Lux plate reader (Thermo Fisher).
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bioRxiv - Biochemistry 2021Quote: ... Detection of relative luciferase units was carried out using a Varioskan Lux plate reader (Thermo Fisher). Percent neutralization was calculated relative to signals obtained in the presence of virus alone for each experiment.
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bioRxiv - Immunology 2022Quote: ... Detection of relative luciferase units (RLUs) was measured using a Varioskan Lux plate reader (Thermo Fisher).
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 3’ end RNA was synthesized by in vitro transcription using an mMESSAGE mMACHINE T7 kit (Invitrogen) with a DNA template (5’- TAATACGACTCACTATAGCTATCCCCATGTGATTTTAATAGCTTCTTAGGAGAAUGACGTAGCATGCTACGCG -3’ ...
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bioRxiv - Physiology 2022Quote: ... Density-gradient-based isolation was then performed using the Total Exosome Isolation kit (Invitrogen) 68 ...
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bioRxiv - Cell Biology 2020Quote: ... or BODIPY™ 558/568 C12 (4,4-Difluoro-5-(2-Thienyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Developmental Biology 2021Quote: Embryos and larvae from 9 hpf to 4 wpf were incubated in 3 µM 5-Ethynyl-2′-deoxyuridine (EdU; ThermoFisher Scientific, cat#: C10639) in FSW for 15-30 min ...
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bioRxiv - Systems Biology 2019Quote: The 72 fractions obtained from the OG fractionation (3 TMT x 24 fractions) were loaded on a trap nanocolumn (0.01 x 2 cm, 5 μm; Thermo Fisher Scientific, Massachusetts, USA) and separated with a C-18 reversed-phase (RP ...
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bioRxiv - Cell Biology 2022Quote: ... siRNA oligonucleotides were obtained as pre-designed siRNAs as follows: MFF-sense strand: 5’-CGCUGACCUGGAACAAGGAdTdT-3’ for exon 2 30 (Ambion, Austin, TX, USA); DLP1-sense strand ...
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bioRxiv - Neuroscience 2022Quote: ... free floating NAc sections were first washed (3 × 5 min) in 1x PBS containing 2% Triton X-100 (PBST) (Thermo Fisher, Waltham, MA). Sections were then blocked in 5% normal goat serum (NGS ...
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bioRxiv - Neuroscience 2019Quote: ... 3 PBS washes were performed and then 2 hours of incubation in CY-5 goat anti-rabbit (1:1000, Invitrogen, ThermoFisher Scientific, A10523) diluted in 2%NGS-PBS ...
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bioRxiv - Bioengineering 2023Quote: ... pRSV-Rev = 5: 3: 2) were co-transfected into HEK-293T cells at a ratio of 1:1 using lipofectamine 3000 (Invitrogen, USA, Cat: #L3000015). After 48 h ...
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bioRxiv - Neuroscience 2023Quote: ... An imaging window was constructed from three layers of microscope cover glass (1 x 5 mm, 2 x 3 mm diameter, Fisher Scientific, no. 1) joined with a UV-curable optical glue (NOR-61 ...
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bioRxiv - Molecular Biology 2019Quote: Three specific 5’ RACE primers and two 3’ RACE primers were designed according to the Race kit instructions (Invitrogen & Clontech) (Table S1) ...
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bioRxiv - Microbiology 2021Quote: ... using a HA-specific primer (5’-GTCCTTGCGACTG-3’) and a RevertAid first-strand cDNA synthesis kit (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... using a HA-specific primer (5’-GTCCTTGCGACTG-3’) and a RevertAid first-strand cDNA synthesis kit (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: 1 μg total RNA from healthy and OS samples was used for 5’ and 3’ RACE reactions with the FirstChoice RLM-RACE kit (Ambion) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... 5’L1-3’L2 orientation were inserted via recombination into a pCS2+ Gateway-converted vector (Custom vector conversion kit; Invitrogen). Details of the Gateway plasmid are available upon request ...
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bioRxiv - Microbiology 2020Quote: ... one or two guanines were added to the 5’ end of the guide sequence within the primer to ensure the format “5’-GG(N18-20)-3’” in order to facilitate in vitro transcription with MEGAscript T7 in vitro transcription kit (Ambion). Transcribed sgRNAs were purified with MEGAclear kit (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: RNA from individual E10.5 hearts (N=3 or 5 per genotype) was isolated using the RNAqueous-Micro Total RNA Isolation Kit following manufacturer recommended protocols (Invitrogen). cDNA synthesis was performed using random hexamers and SuperScript IV reverse transcriptase (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... was synthesized on templates that contain the T7 promoter sequence (5’-TAATACGACTCACTATAGGGTACT-3’) at each end using a MEGAscript T7 kit (Ambion); templates were amplified from 0–4 h embryonic cDNA using specific primers (5’-AGCAGATGACGACGACAACA-3 ...
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Mechanisms of transcriptional regulation in Anopheles gambiae revealed by allele specific expressionbioRxiv - Genetics 2023Quote: RNA was extracted from pools of 10 female F1 progeny from each of the six crosses 3-5 days after eclosion using RNAqueous4PCR total RNA isolation kit (Invitrogen). RNA quality and quantity was checked to be adequate for library preparation using the Agilent Bioanalyser profile and Qubit® 2.0 Fluorometer ...
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bioRxiv - Immunology 2019Quote: ... from RNA extracted from peripheral blood mononuclear cells (PBMCs) utilizing the following primers: forward 5’-GAGAATTCACCATGACTATGGAGACCCAAATG-3’ and reverse 5’-CGTACGCCCCATTGGTGAAGAGCTGCC-3’ (Thermo Fisher Scientific, Waltham, MA, USA). PCR product was fused by restriction enzyme-compatible ends with the CD8α-CD28-CD3ζ domain contained in the pcDNA3.1/V5-His TOPO TA (Invitrogen ...