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Citations for Thermo Fisher :
4901 - 4950 of 6235 citations for Serpin B6 Human Trx His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Human islet and hESC-derived differentiated organoids (approximately 20 organoids or human islets per well) were respectively plated into a 96-well black plate (ThermoFisher) coated with 1% hESC-qualified Matrigel ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were loaded with 5 µg/µL of the Ca2+-sensitive dye Fluo-4 (1923626, Invitrogen, Thermo Fisher Scientific) for 60 min before being transferred to a recording chamber ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were loaded with 5 µg/µL of the Ca2+-sensitive dye Fluo-4 (1923626, Invitrogen, Thermo Fisher Scientific) for 60 min before being transferred to a recording chamber ...
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bioRxiv - Microbiology 2020Quote: ... human serum or oseltamivir were serially two-fold diluted in Dulbecco’s phosphate buffered saline with calcium and magnesium (ThermoFisher Scientific) containing 1% BSA (Sigma ...
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bioRxiv - Biophysics 2021Quote: ... ATCC) and HeLa (a human cervical cancer cell line, ATCC) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, USA) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin ...
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bioRxiv - Immunology 2021Quote: ... Primary antibodies were diluted 1:100 in PBS-T as follows: single labeling: CD14 (mouse anti-human, 14-149-82, Invitrogen), CD56 (mouse anti-human ...
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bioRxiv - Microbiology 2021Quote: ... the bound antibody was detected with goat anti-human Ig (γ chain specific)-HRP conjugated secondary antibody (Thermo Fisher Scientific) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were treated with 10 μg/mL mouse monoclonal anti-human IFNAR2 (ThermoFisher Scientific, MMHAR-2), or human IgG (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from human cells (1 x 106 cells) using the TRIZOL reagent according to the manufacturer’s instructions (ThermoFisher Scientific). RNA integrity for each sample was confirmed with by Nano Drop ...
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bioRxiv - Microbiology 2021Quote: ... if sequences diverged between strains) were codon-optimised for expression in human cells and synthesised by GeneArt (Thermo Fisher Scientific), with an optimal 5’ Kozak sequence and fused to an N-terminal FLAG epitope ...
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bioRxiv - Immunology 2020Quote: ... and human fibroblasts (HF) clone EF-1-F (sourced from Doorbar lab, University of Cambridge) were grown in MEM (Gibco) supplemented with 10 % FBS ...
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bioRxiv - Microbiology 2020Quote: ... and human hepatoma Huh7.5 cells83 were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (Invitrogen, Paisley, UK) supplemented with 10% heat inactivated fetal bovine serum (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with 500ng of a subset of ACE2 expression constructs (human, hamster, rabbit, pig, chicken, horseshoe bat) or mock transfected with an empty vector (pDISPLAY) in OptiMEM (Gibco) using TransIT-X2 transfection reagent (Mirus ...
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bioRxiv - Microbiology 2021Quote: ... Caco-2 (human epithelial colorectal adenocarcinoma, kindly provided by Prof. Holger Barth, Ulm University) cells were cultivated in DMEM (Gibco) containing 10% FBS (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... HEK-293T overexpressing the human ACE2 were kindly provided by Integral Molecular Company and maintained in DMEM with 1 μg/mL of puromycin (Invitrogen). TMPRSS2 human plasmid (Origene ...
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bioRxiv - Microbiology 2021Quote: ... on HEK-293T overexpressing the human ACE2 and maintained in the previously described media containing 1 mg/mL of geneticin (Invitrogen) to obtain TMPRSS2/ACE2 HEK-293T cells ...
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bioRxiv - Molecular Biology 2020Quote: Mammalian expression plasmids encoding SARS VHH72 and MERS VHH-55 (73) with a C-terminal a monomeric human Fc tag were transfected into ExpiCHO cells (Thermofisher) using ExpiFectamine as manufacterer’s recommendation ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... followed by internalization into target cells and detection by incubation with secondary anti-human-Alexa 555 fluorophore conjugates (Thermo Scientific). Cells were examined by immunofluorescence analysis performed on an Axio Observer Z1 widefield epifluorescence microscope (Zeiss Inc. ...
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bioRxiv - Microbiology 2021Quote: ... and HCT 116 p53- /- (human, kind gift from Dr. Wilson) were grown in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplied with 10% FBS (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... HUVECs were cultured in a mixture of 199 medium and SFM (Human Endothelium Serum-Free Medium, GIBCO, cat. No. 11111044), with 10% of FBS (Biowest ...
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bioRxiv - Cancer Biology 2021Quote: Two biological replicates of RNA samples were isolated from A549 cells transduced with pMSCV-dnCRTC or pMSCV-GFP retroviruses at 72 hours post-infection and were then subjected to microarray experiment using GeneChip® Human Transcriptome Array 2.0 (Affymetrix) at the Genomics Core at Sanford Burnham Research Institute ...
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bioRxiv - Cancer Biology 2021Quote: ... Gene expression data was collected using the Gene Chip® Human Genome U133 Plus 2.0 Array (Affymetrix, Santa Clara, CA) protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... the slides were incubated with anti-human IgG1-AlexaFluor 594 or streptavidin Alexa Fluor 594 (Life Technologies-Thermo Fisher Scientific) diluted in PBS + 1% BSA buffer for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... the slides were incubated with anti-human IgG1-AlexaFluor 594 or streptavidin Alexa Fluor 594 (Life Technologies-Thermo Fisher Scientific) diluted in PBS + 1% BSA buffer for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were from A Human Glioblastoma Cell Culture Resource (HGCC) and were cultured as described (Xie et al., 2015) in Neurobasal medium (GIBCO)/DMEM/F12 with Glutamax (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... RWPE-1 (human normal prostate epithelial cells, CRL-11609, ATCC) cells were cultured in Keratinocyte Serum Free Medium (17005042, Gibco) supplemented with 0.05 mg/ml bovine pituitary extract ...
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bioRxiv - Cell Biology 2021Quote: ... ON-TARGET plus human MGEA5 (10724) siRNA-smartpool) or nontargeting siRNA (Dharmacon, ON-TARGETplus nontargeting pool) using Lipofectamine RNAiMAX (Invitrogen) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... hiPSC-CMs were washed 3 times with PBS 1X and incubated with the secondary antibodies diluted in blocking solution: goat anti-Human Alexa Fluor 647 (1:400; A-21445 - Invitrogen) and goat anti-Mouse Alexa Fluor 488 (1:400 ...
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bioRxiv - Cell Biology 2020Quote: ... and ESRG_cn2 (Hs06675423_cn) detected the ESRG locus and TaqMan Copy Number Reference Assay human RNase P (4403326, Thermo Fisher Scientific) was used as an endogenous control ...
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bioRxiv - Cell Biology 2020Quote: ... and target genes were amplified for 12 cycles of PCR using the Ion AmpliSeq Human Gene Expression Core Panel (Thermofisher). This panel contains a pool of 20,802 amplicons (41,604 primers ...
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bioRxiv - Biophysics 2020Quote: Expression was conducted according to the manual of 1-Step Human High-Yield Mini in vitro translation (IVT) kit (Product No. 88891X) distributed by ThermoFisher Scientific (Pierce Biotechnology ...
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bioRxiv - Cancer Biology 2021Quote: ... and Non – metastasis human colon cancer cell line (HT-29) were cultured in Dulbecco’s modified Eagle’s medium (DMEM: Gibco, Thermo Fisher, USA) and metastasis human colon cancer cell line (SW-620 ...
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bioRxiv - Cell Biology 2020Quote: Parent CHO-K1 and CHO-K1 cells stably expressing extracellular ACP-tagged human insulin receptors were maintained at 37°C with 5% CO2 in HAM’s F-12 medium (Thermo Fisher) supplemented 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2021Quote: Human ProtoArray® Microarray (v5.0) containing 9483 unique human proteins expressed as GST-fusion in insect cells were purchased from Invitrogen. Arrays were blocked in 4°C cold blocking buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Developmental Biology 2022Quote: ... These zona-free eggs were incubated with 200 nM Fc-fusion proteins in Biggers-Whitten-Whittingham (BWW) medium for 1 h and then stained with goat anti-human IgG Fc antibody DyLight 488 (Invitrogen) at a dilution of 1:50 for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were washed and stained for flow cytometry using appropriate seconday antibodies: goat anti-human fluorescein isothiocyanate (FITC; ThermoFisher A18812), anti-mouse Alexa Fluor 488 (Cell Signaling Technology 4408S) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells and NK cells were isolated from cryopreserved PBMCs using commercial kits (MagniSort Human CD4+ T Cell Enrichment; Affymetrix, and MagniSort™ Human NK cell Enrichment ...
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bioRxiv - Biochemistry 2022Quote: ... After 3hrs from release of the nocodazole block cells were double transfected with pEGFP-human LA (EGFP-wt LA and mutants) and RFP-HP1α using Lipofectamine 2000 (Invitrogen) in accordance with the manufacturer’s protocol at early passages having 60-70% confluency and were kept in culture for 24 hrs.
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bioRxiv - Cell Biology 2022Quote: The human cDNA encoding zDHHC2 was codon optimised for protein expression in insect cells and synthesized by GeneArt technology (Invitrogen). It was inserted with N and C-terminal tags (His6 and StrepII ...
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bioRxiv - Bioengineering 2022Quote: ... immortalized human dermal fibroblast cell line (Stephens et al., 2004) were cultured in Dulbecco’s modified Eagle medium (DMEM, Gibco™) supplemented with 10% fetal bovine serum (FBS ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... from the RUES2 line (RUESe002-A; WiCell) were maintained on recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) in Essential 8 (E8) media (ThermoFisher) at a density of 0.5 µg/cm2 ...
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bioRxiv - Microbiology 2022Quote: ... Antibody binding was detected using goat anti-Human IgG Fc Secondary Antibody conjugated to horse radish peroxidase (Thermo Fisher Scientific), diluted 1:5000 in blocking solution and incubated for 1 hr at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... Paraffin-embedded human tissues and hind paws were cut into 5 μm sections with the Microtome HM355S (Thermo Fisher Scientific) and transferred onto microscope slides.
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bioRxiv - Immunology 2022Quote: For RNA extraction from human lung resection material 50∼100 mg of RNAlater-stabilized lung tissue pieces were added to 1ml of Trizol (Invitrogen) and homogenized ...
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bioRxiv - Neuroscience 2022Quote: DNA coding for full length HA-tagged human NEGR1 (NM_173808.2) was synthesized using the GeneArt® Gene Synthesis service (Life Technologies) and subcloned into the pcDNA3 vector ...
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bioRxiv - Microbiology 2022Quote: J774.1 mouse macrophages (ATCC) or primary neonatal normal human dermal fibroblasts (Neo-NHDF) (ATCC) were cultured in 10 mL DMEM (Gibco, ThermoFisher) in T75 flasks (VWR ...
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bioRxiv - Microbiology 2022Quote: To evaluate expression levels of A24Rwt and A24Rcd, human 293T cells (105 cells, 12-well plates, triplicates) were transiently transfected using Lipofectamine 2000 (LPF2000, Invitrogen) with 1 µg of pCAGGS expression plasmids encoding C-terminal HA-tagged A24Rwt or A24Rcd ...
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bioRxiv - Bioengineering 2022Quote: ... zsGreen/Oatp1b3) into human embryonic kidney (HEK 293T) cells using Lipofectamine 3000 according to the manufacturer’s lentiviral production protocol (Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2022Quote: ... and then incubated with a 1:10000 dilution (in PBST with 1% BSA) of goat anti-human Fc HRP-conjugated antibody (Invitrogen) at room temperature for 1 hour ...
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bioRxiv - Genomics 2022Quote: Cardiac differentiation of human iPS cells was performed using a stage-based protocol in RPMI-1640 (ThermoFisher Scientific, Waltham, MA) supplemented with 0.5 mg/mL recombinant human albumin (Sigma-Aldrich ...