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Citations for Thermo Fisher :
4751 - 4800 of 6235 citations for Serpin B6 Human Trx His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: WTC-mTagRFPT-LMNB1 human iPSCs were dissociated into single cells with 5 min incubation in Accutase (Life Technologies, #A1110501) at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Each pool was labeled and hybridized to Affymetrix® Human Gene 2.1 ST according to the manufacturer instructions (Affymetrix). Quality control and normalization of Affymetrix.CEL files using the Robust Multi-array Average (RMA ...
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bioRxiv - Immunology 2023Quote: ... and TNF-α (cat#BMS223-4) were measured in duplicate using human ELISA kits following the manufacturer’s instructions (ThermoFisher) and SpectraMax M3 (Molecular devices).
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bioRxiv - Genomics 2023Quote: ... falciparum clone II313 was maintained in human A+ erythrocytes at 37 °C in RPMI 1640 medium containing AlbumaxII (Invitrogen) supplemented with 2 mM L-glutamine ...
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bioRxiv - Developmental Biology 2023Quote: ... and/or ALK1 (SMARTpool ON-TARGETplus Human ACVRL1 siRNA, Dharmacon L-005302-02-0005) using Lipofectamine 3000 (ThermoFisher L3000015) according to manufacturer directions ...
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bioRxiv - Microbiology 2023Quote: Human embryonic kidney 293T cells (HEK293T) and Vero E6 cells were maintained in Dulbecco modified Eagle’s medium (DMEM; Gibco) with 8% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... after which they were washed with 1% human albumin in PBS and stained with propidium iodide (Molecular Probes #P3566) for assessing viability ...
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bioRxiv - Cell Biology 2023Quote: ... The digested tissue was mixed with an equal volume of 1% human albumin (CSL Behring) in DPBS −/− (Gibco #14190094) to stop the lysis ...
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bioRxiv - Neuroscience 2023Quote: ... engraftment was measured weekly by quantifying human CD45+ cells in each mouse’s peripheral blood through fluorescence-activated cell sorting (FACS) on Attune flow cytometer (ThermoFisher). On average ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... and seeded onto new 6-well plates at 2,500 cells/cm2 with 1.5 μg/cm2 of truncated recombinant human vitronectin (Vtn-N; ThermoFisher #A14700). After allowing cells to adhere overnight ...
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bioRxiv - Microbiology 2023Quote: ... Cat#40590-D001) and anti-human AF488 (1:1000 with final concentration of 2μg/ml; ThermoFisher Cat#A-11013) for secondary conjugation ...
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bioRxiv - Immunology 2023Quote: Human NK cells were seeded at a density of 2×106 cells/mL in a 24-well plate (NUNC) in the presence of IL-15 (10 ng/mL ...
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bioRxiv - Bioengineering 2023Quote: Human breast cancer cell lines MDA-MB-231 (ATCC) and MCF-7 (ATCC) were grown in DMEM/F12 (Gibco) with 15 mM HEPES supplemented with 10% v/v fetal bovine serum (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... Transferrin (loading control) was detected with mouse anti-human transferrin receptor antibody (1:500, Cat# 136800, Invitrogen, Waltham, MA). Protein samples (50 µg ...
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bioRxiv - Neuroscience 2023Quote: ... Amyloid Aβ40 and Aβ42 in the hippocampus and striatum and D2R in the striatum were quantified by ELISA with the following kits: Aβ40 human ELISA kit (Thermofisher); Aβ42 human ultrasensitive ELISA Kit (Thermofisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... with the human dystrophin 3′ UTR or mutant 3′ UTR and with 50 nM miR-146a mimic (Life Technologies) with Lipofectamine 2000 according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Genetics 2024Quote: ... 3% human serum (heat-inactivated from Sigma-Aldrich, St. Louis, MO, USA or Thermo Fisher Scientific, Waltham, MA, USA), 2% human plasma (isolated from umbilical cord blood provided by Stanford Binns Cord Blood Program) ...
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bioRxiv - Cell Biology 2023Quote: ... and separately two Silencer Select siRNAs targeting human Myosin ID (4392420; ID s9203 and s9204; Invitrogen, Thermo Fisher Scientific) at a final concentration of 0.01 uM using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Systems Biology 2024Quote: MS raw data were searched against the UniProtKB human sequence data base (92,954 entries) and TMT 10plex quantitation performed using Proteome Discoverer software (v 2.3; Thermo Scientific). Search parameters were set to include carbamidomethyl (C ...
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bioRxiv - Microbiology 2024Quote: Human cervix adenocarcinoma cells (HeLa 229, ATCC CCL-2.1TM) were cultured in RPMI-1640 medium (Thermo Fisher Scientific, 72400054) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then treated with human serum containing phospholipase D (Australian Red Cross Lifeblood) or 0.25% (w/v) Albumax II (Gibco) and incubated for 3 hours at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and separately two Silencer Select siRNAs targeting human Myosin ID (4392420; ID s9203 and s9204; Invitrogen, Thermo Fisher Scientific) at a final concentration of 0.01 uM using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: HeLa human cervical cancer cells and HepG2 human hepatocellular carcinoma cells were cultured in high-glucose Dulbecco’s modified Eagle medium (DMEM, Gibco) supplemented with 4.5 g/L glucose ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Tg tachyzoites were maintained in vitro by serial passage on confluent monolayer of human foreskin fibroblasts (HFF, ATCC SCRC-1041) in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 1% (vol/vol ...
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bioRxiv - Molecular Biology 2023Quote: The human RPE cell line ARPE-19 was cultured in DMEM/F-12 (Cat #C11330500BT, Gibco, Waltham, MA, USA) media with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... DRG expression levels were determined from total RNA extracts measured using Affymetrix’ Human Transcriptome Array 2.0 (Affymetrix, Santa Clara), scanned with Affymetrix’ GeneChip 3000 G7 Instrument System ...
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bioRxiv - Neuroscience 2023Quote: ... Aβ concentrations in three separate fractions of brain homogenates were determined by Invitrogen™ Amyloid β 42 and 40 Human ELISA kits (Cat.-No.: KHB3441 and KHB3481, respectively; both from Thermo Fisher Scientific). Results were normalized on the basis of the sample’s protein concentration.
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bioRxiv - Microbiology 2023Quote: The adult human dermal fibroblast cell line (HDFα, FH1092, FuHeng Biology) was cultured in RPMI 1640 medium (11875093, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... were propagated in 5% of human O+ erythrocytes and 10 mL of RPMI-1640 medium containing 0.5% Albumax II (Invitrogen), 2 mM L-glutamine ...
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bioRxiv - Microbiology 2023Quote: All parasite lines were maintained in human foreskin fibroblasts (HFFs; ATCC) in Minimal Essential Media-α (MEM-α; Gibco) supplemented with 7% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: The gene sequences of human MCHR1(residues 70-405) and MCHR2(residues 1-331, with L2566.40Y mutation) was subcloned into pFastBac vector (Invitrogen) with an N-terminal haemagglutinin signal peptide (HA ...
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bioRxiv - Biochemistry 2023Quote: ... The level of IL-8 in cell culture supernatants was determined using a human IL-8 ELISA kit (Invitrogen).
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bioRxiv - Biochemistry 2023Quote: Human embryonic kidney (HEK293) and A549 cell lines were maintained in Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific, Lithuania) containing 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA coding for the protein sequences was synthesized using codon-optimization for human host cells (Thermo Fisher Scientific). If the codon optimization for the lysine-stretches resulted in six or more consecutive adenines in the DNA ...
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bioRxiv - Microbiology 2023Quote: Mouse fecal pellet and human stool sample data analysis was performed using FreeStyle software (version 1.8 SP2, Thermo Scientific). Metabolite identification was established by matching accurate mass ...
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bioRxiv - Biophysics 2023Quote: ... The plate was then washed and incubated with horseradish peroxidase-conjugated anti- human IgG (InvitrogenTM, ThermoFisher Scientific, Waltham, MA) at a dilution of 1:2000 for 2h at room temperature ...
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bioRxiv - Microbiology 2023Quote: The human-induced pluripotent cells (hiPSCs) used in this study were reprogrammed from adult dermal fibroblasts (HDFa, Gibco, C0135C) using the CytoTune-iPS 2.0 Sendai Reprogramming Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... murine or human (Panc-1) PDAC cells were stained in suspension with CPD670 (#65-0840-85, Thermo Fisher Scientific). After labeling ...
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bioRxiv - Bioengineering 2023Quote: ... Protein expression was achieved by transient transfection of FreeStyle human embryonic kidney 293 (HEK293-F) cells (Thermo Fisher Scientific). Cells were grown in non-baffled shake flasks (Corning ...
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bioRxiv - Microbiology 2023Quote: ... The human Rab6a coding sequence (DNASU Plasmid Repository, #HsCD00296778, NCBI Nucleotide reference BC096818) was inserted by Gateway recombination (Invitrogen) to make an in-frame EmGFP-Rab6a fusion.
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bioRxiv - Immunology 2023Quote: ... CFSE-stained T cells were resuspended at 1e6/ml and DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) were added at a ratio of 1:10 beads:cells ...
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bioRxiv - Biochemistry 2024Quote: The full-length wild-type human NTCP gene (UniProt ID: Q14973) was generated synthetically by GeneArt (Thermo Fisher Scientific) after codon optimization for expression in HEK293 cells ...
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bioRxiv - Neuroscience 2023Quote: Human dermal fibroblasts were obtained from skin biopsies and cultivated in Dulbecco’s modified eagle medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... ∼100,000 human bladder epithelial cells (RRID: CVCL_0126) were seeded in 24-well plates overnight in RPMI (Gibco; ThermoFisher Scientific) supplemented with 10% heat inactivated FBS ...
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bioRxiv - Microbiology 2024Quote: ... ∼100,000 human bladder epithelial cells (RRID: CVCL_0126) were seeded in 24-well plates overnight in RPMI (Gibco; ThermoFisher Scientific) supplemented with 10% heat inactivated FBS ...
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bioRxiv - Immunology 2024Quote: IFNγ in supernatants from the whole blood RSA was determined by ELISA according to the manufacturer’s instructions (Human IFNγ Uncoated ELISA kit; Invitrogen). 50μl of supernatant was diluted with 50μl of assay diluent for use in the IFNγ ELISA ...
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bioRxiv - Immunology 2024Quote: Primary human PBMCs and CAR T derivatives thereof were cultured in RPMI 1640 (Gibco, Life Technologies, Carlsbad, CA, USA) with 10% FCS ...
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bioRxiv - Immunology 2024Quote: Primary human PBMCs and CAR T derivatives thereof were cultured in RPMI 1640 (Gibco, Life Technologies, Carlsbad, CA, USA) with 10% FCS ...
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bioRxiv - Physiology 2024Quote: ... The PCR amplification of human Piezo1 was performed using a Veriti Thermal Cycler (Thermo Fisher Scientific, Waltham, MA, USA) and ExTaq (Takara ...