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Citations for Thermo Fisher :
4901 - 4950 of 10000+ citations for Fatty acid binding protein FABP1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The coding sequences of a five glycine linker and intracellular GFP and biotin tags followed and were inserted in a pcDNA3.1(-)/myc-His (Invitrogen) vector backbone ...
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bioRxiv - Genetics 2024Quote: ... and 15 µL of each eluate was mixed with the same volume of Hi-Di formamide (Thermo Fisher Scientific). Samples were finally sequenced either in one or in both directions on a 3500 Genetic Analyzer device (Applied Biosystems/Hitachi ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant AtGNL-6XHIS was detected using an anti-HIS (C-term)/AP antibody at a 1:2,000 v/v dilution (Invitrogen) and CDP-start ...
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bioRxiv - Biophysics 2023Quote: ... and from the 3’-terminus by the 10×His-tag nucleotide sequence and inserted into a pFastBac1 vector (Invitrogen) via the BamHI(5’ ...
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... Sequencing was done using the Ion PI™ Hi-Q™ Sequencing 200 Kit (Thermo Fisher Scientific, Catalog # A26772) on Ion Proton sequencer with sequencing data processing using the Torrent Suite TM Software (Ver ...
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bioRxiv - Biophysics 2023Quote: ... and from the 3’-terminus by 10×His-tag nucleotide sequence and inserted into the pFastBac1 vector (Invitrogen, USA) via the BamHI(5’ ...
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bioRxiv - Developmental Biology 2022Quote: ... mCherry-Emp-V5His construct was cloned using Hifi DNA assembly kit (NEB E5520S into the pAc5.1/V5-His A vector (Invitrogen) using the following enzymes Acc65I and XhoI ...
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bioRxiv - Immunology 2023Quote: HCMV US18 and US20 were amplified from the HCMV TB40/E BAC (accession #EF999921) and subcloned into pcDNA3.1-V5/His (Invitrogen) via the KpnI/NotI sites to generate pcDNA3.1 US18-V5/His and pcDNA3.1 US20-V5/His ...
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bioRxiv - Molecular Biology 2023Quote: ... ceSMSγ and ceSMSr cDNAs were PCR amplified and ligated into the copper-inducible pMT/V5-His B vector (Invitrogen).
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bioRxiv - Genetics 2023Quote: ... standard fragment analysis conditions) with 0.8 ul PCR product is loaded in 9.4 ul Hi-Di Formamide (Applied Biosystems), with 0.1 ul GeneScan 500 LIZ (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... and gB[H527P]-His EVs was performed at 300 keV on a Titan Krios electron microscope (Thermo Fisher scientific) equipped with a Gatan K3 (5 μm/pixel ...
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bioRxiv - Microbiology 2023Quote: ... we initially digested the pNL4-3 plasmid with EcoRI and XhoI and subcloned this fragment into pcDNA3.1/myc-His A (Invitrogen). To generate pcDNA3.1 NL4-3 Env TMTR ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged TpPyShell together with TEV protease was dialyzed overnight at 4°C in SnakeSkin dialysis tube (Thermo Scientific) against dialysis buffer (50 mM Tris-HCl pH8.0 ...
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bioRxiv - Immunology 2023Quote: ... Amplification and cloning of the truncated versions of TGM4 (D1-3 and D4-5) was performed by PCR amplification using proofreading Taq polymerase Phusion Hi as per the manufacturer’s instructions (Invitrogen), full-length codon-optimised TGM4 as template ...
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bioRxiv - Biochemistry 2024Quote: ... The membrane was incubated overnight at 4°C with anti-His-Tag antibodies (Thermo Fisher Scientific, USA, #MA1-21315) diluted 1:1000 in the blocking solution ...
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bioRxiv - Biochemistry 2024Quote: ... followed by a 6× His-tag was cloned into the BamHI and XbaI sites of the pcDNA 3.1 vector (#V79020, Invitrogen) containing the vascular endothelial growth factor receptor 1 (VEGFR ...
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bioRxiv - Genetics 2024Quote: ... together with internal size standard ladder where 0.8 µl PCR product was loaded in 9.4 µl Hi-Di Formamide (Applied Biosystems), with 0.1 µl GeneScan 500 LIZ (Applied Biosystems) ...
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bioRxiv - Biochemistry 2022Quote: ... The antibody-protein complexes were isolated by incubation with magnetic beads (Invitrogen Dynabeads protein A and protein G) for 6 hours at 4℃ ...
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bioRxiv - Physiology 2023Quote: ... supernatant protein concentration was determined by BCA protein assay (Pierce BCA Protein Assay Kit, Cat#23225, Thermo Scientific). Twenty μg/lane was run (200V ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant containing nuclear proteins was measured for protein concentration using Pierce BCA Protein Assay Kit (Thermo Scientific) and stored at -80°C until use ...
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bioRxiv - Bioengineering 2023Quote: ... Total protein concentration was determined using the Qubit™ Protein and Protein Broad Range (BR) Assay Kit (Invitrogen).
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bioRxiv - Bioengineering 2023Quote: Protein yield: MSC-EV preparation total protein concentration was determined using the Micro BCA Protein Assay Kit (ThermoFisher) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration of each sample was determined using the BCA protein assay (Pierce™ BCA Protein Assay, Thermofisher). 40 micrograms of protein per lane were prepared in SDS sample buffer (50 mM Tris pH 6.8 ...
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bioRxiv - Neuroscience 2024Quote: Protein concentration in the protein sample was determined by using a BCA protein assay kit (Pierce, Thermo Scientific). Samples in aliquots of 100 μL each were stored at -80°C.
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bioRxiv - Plant Biology 2019Quote: The GAL4 DNA-binding domain vector pGBDKT7-GWRFC was constructed by insertion of the Gateway reading frame cassette RfcC (Invitrogen) into the Klenow-Fragment-treated NdeI-SalI sites of pGBKT7 (pGBDKT7 ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... The antibodies were diluted in Milk/TBST (5% non-fat dry milk in TBS with 0,1% Tween20) and antibody binding was evaluated using the SuperSignal West Dura Extended Duration Substrate (ThermoFisher Scientific) or SuperSignal West Femto Maximum Sensitivity Substrate (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... A TALEN pair selected for in vivo Arhgef3 editing (see Figure 1A for TALEN binding sites) was linearized with ApaI and transcribed into mRNA using the mMESSAGE mMACHINE SP6 transcription Kit (Invitrogen). The RNA was purified with the MEGAclear Transcription Clean-Up Kit (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA binding reactions were performed according to LightShift EMSA Optimization and Control Kit protocol (Pierce Biotechnology, ThermoFisher Scientific; Cat# 20148). Binding reactions—using 2μl of GST (4.6 μg/μl) ...
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... Cells were then plated into a well of a low-cell-binding V-bottom 96-well plate (Thermo Fisher Scientific) at 3500 cells per well in GK15 medium supplemented with 200 ng/ml BMP4 (R&D Systems ...
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bioRxiv - Developmental Biology 2021Quote: ... in low binding Eppendorf tubes pre-filled with 100µL of Extraction Buffer from Arcturus PicoPure RNA isolation Kit (Applied Biosystems). Samples were frozen and stored at -80°C for later use ...
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bioRxiv - Microbiology 2022Quote: Unspecific Fc binding was blocked for 15 minutes on ice with unconjugated anti-CD16/CD32 (clone 93; Thermo-Fisher Scientific). Cells were subsequently fluorescently labeled for 30 minutes on ice with the following antibodies and reagents ...
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bioRxiv - Cell Biology 2022Quote: ... or 1 μg of Super8-FOPflash (8 mutant TCF-binding sites) reporter plasmids (kindly provided by Randall Moon, University of Washington, Seattle) using Lipofectamine 2000 (Invitrogen); a thymidine kinase-Renilla plasmid (0.1 μg ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were excluded using ThermoFisher LIVE/DEAD Fixable Aqua Dead Cell Stain and binding to Fc receptors was blocked using CD16/CD32 (clone 93, eBioscience/ThermoFisher). Absolute cell numbers were calculated using counting beads (123count eBeads ...
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bioRxiv - Immunology 2021Quote: ... Induced yeast libraries were stained for antigen binding (RBD-Fc APC tetramers) and scFv expression (chicken anti-c-myc IgY; Invitrogen). Following two washes in PBSF (1x PBS ...
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bioRxiv - Genetics 2020Quote: ... Intracellular staining (for experiments in which the NF-κB binding site containing rs6927172 was deleted) was performed using the eBioscience Foxp3 / Transcription Factor Staining kit (ThermoFisher) and a Phospho-IkB alpha (Ser32 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Samples were diluted with 400 μL Annexin V binding buffer and acquired on an Attune NxT flow cytometer (ThermoFisher Scientific). A minimum of 10,000 events were acquired in each sample ...
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bioRxiv - Biophysics 2019Quote: ... Cells were then washed three times in binding buffer by centrifugation and subsequently incubated with Alexa Fluor 647 streptavidin conjugate (Invitrogen) for 15 minutes at 4° C ...
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bioRxiv - Bioengineering 2020Quote: ... Epitope confirmation was done by a competitive binding assay where saturating concentration of ICR-10 PE antibody (Thermo Fisher Scientific) was coincubated with a concentration gradient of unlabeled E1-1DD or E4-1DD ...
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bioRxiv - Microbiology 2021Quote: ... The secondary antibody binding was detected by adding 50 μL of tetramethylbenzidine (TMB) peroxidase substrate (cat# 34022, Thermo Fisher Scientific). The peroxidase reaction was terminated by adding 50 μL of H2SO4 (1 M ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then incubated with 1% BSA in PBS for 30 min to block non-specific binding prior to staining F-actin with rhodamine-conjugated phalloidin (Invitrogen) for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... were added to 200-400 μl lysis/binding solution in the RNAqueous micro-scale phenol-free total RNA isolation kit (Ambion). Trace contaminating DNA was removed with TURBO DNA-free (Ambion) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cell pellets were re-suspended in binding buffer (500 μL) and stained with Annexin V-FITC and PI using a Dead Cell Apoptosis Kit (Invitrogen) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2019Quote: ... Non-specific binding sites were blocked for 2 h at room temperature with 5% normal SVF (Gibco, ThermoFisher Scientific, France) in 0.1% Triton X-100-PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Non-specific binding sites were blocked for 2 h at room temperature with 5% normal SVF (Gibco, ThermoFisher Scientific, France) in 0.1% Triton X-100-PBS ...
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bioRxiv - Cell Biology 2021Quote: ... neuro-retinal vesicles (NRVs) were manually excised using 21G needles/scalpel blades and grown in low-binding 96 well plates (96 well Nunc Sphera Round Bottom Plates ...
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bioRxiv - Cancer Biology 2021Quote: ... The antibodies were diluted in Milk/TBST (5% non-fat dry milk in TBS with 0,1% Tween20) and antibody binding was evaluated using the SuperSignal West Dura Extended Duration Substrate (ThermoFisher Scientific) or SuperSignal West Femto Maximum Sensitivity Substrate (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2022Quote: ... and cloned into the pDEST32 vector containing the GAL4 DNA binding domain (DBD) through Gateway cloning (ThermoFisher, 11789020 and 12538120) and used as the Y2H bait construct ...
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bioRxiv - Neuroscience 2019Quote: ... The non-specific binding of antibodies was avoided by incubating the cells with 5% normal goat serum(Thermo Fisher Scientific)/ 0.4% Triton X-100(Sigma)/1% Glycine(Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... Antibody binding was visualized on CL-XPosure film using ECL SuperSignal West Extended Duration Substrate (both from Thermo Fisher Scientific) or using the Odyssey CLx Imaging System (LI-COR Biosciences) ...
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bioRxiv - Neuroscience 2021Quote: Messenger RNA was isolated from isolated cells by cell lysis in the lysis/binding buffer and by using oligo(dT)25-linked Dynabeads (Invitrogen). The beads with adherent mRNA were suspended in DEPC-treated water (20 µl) ...