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Citations for Thermo Fisher :
4951 - 5000 of 10000+ citations for Fatty acid binding protein FABP1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... and non-immunological binding of the specific immune sera were blocked by 3% bovine serum albumin (Thermo Fisher Scientific Inc.). The indirect method was introduced to immunohistological staining ...
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bioRxiv - Microbiology 2021Quote: ... and the phosphate concentration is determined from the fluorescence increase through binding of phosphate to the phosphate sensor (0.5 µM; Thermo Fisher). PdeL and c- di-GMP concentrations were used ...
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bioRxiv - Plant Biology 2020Quote: Regions screened for transcription factor binding via Yeast One-Hybrid were first inserted into pENTR 5’TOPO TA entry vector (Thermofisher) and subsequently placed into the pMW2 and pMW3 destination vectors containing HIS3 and LACZ marker genes respectively (Deplancke et al. ...
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bioRxiv - Neuroscience 2019Quote: ... sections were washed 3x in TBS then blocked for non-specific binding in TBS containing 10% normal goat serum (Invitrogen) supplemented with 0.1% Triton-X100 for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in 300 μl of Binding Buffer with 2 μg of biotin-labeled tubulin peptide (K40 only, K40-Ac and K40-me3) (ThermoFisher), and samples were rotated at 4 °C for 2 h ...
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bioRxiv - Biochemistry 2021Quote: Four rounds of panning were used to isolate scFvs binding both MERS-CoV S2 and SARS-CoV-2 spike using the following solutions coated on high binding plates: 2 μg/mL anti-c-myc tag antibody (Invitrogen) to eliminate phage expressing no or truncated scFv (Round 1) ...
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bioRxiv - Biochemistry 2020Quote: ... of interest were immobilized at defined concentrations (specified in each context) in pH 9.6 carbonate buffer overnight at 4°C onto high-binding plate (Nunc Maxisorp). Next day ...
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bioRxiv - Immunology 2020Quote: ... Each well was washed and binding of biotinylated monoclonal antibodies was determined with a 1:30000 dilution of HRP-conjugated streptavidin (ThermoFisher). HRP was detected with TMB and stopped with 1% HCl ...
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bioRxiv - Biochemistry 2022Quote: ... were used to generate an amplicon of the insert containing T7 RNA polymerase binding sites from which gene-specific dsRNAs were synthesised by in vitro transcription (T7 Megascript, Thermofisher). DNase1-digested dsRNAs were purified by phenol/chloroform extraction ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were added with a DNA-binding dye 4′,6-diamidino-2-phenylindole (DAPI, 1 µg mL-1 in PBS, Invitrogen) and phalloidin conjugated Alexa Fluor dye (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Binding of the primary antibody was detected by an enhanced chemiluminescence method (SuperSignal™ West Femto, 34094, Thermo Fisher Scientific) using horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit IgG ...
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bioRxiv - Cell Biology 2024Quote: ... Non-specific binding was inhibited by incubating for 2 h in blocking buffer (5% BSA or Goat Serum (Invitrogen, #500622) + Triton X-100 (VWR ...
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bioRxiv - Microbiology 2022Quote: ... The samples from each species were pooled and filter-sterilized through a 0.22 µm low-binding PES membrane using Rapid Flow Rapid-Flow Sterile Disposable Filter Units (Nalgene/Thermo Scientific). The samples were frozen at −80 °C and freeze dried ...
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bioRxiv - Microbiology 2022Quote: ... in PBS were added to the wells of streptavidin-coated high binding capacity 96-well plates (Thermo Fisher Scientific, USA). After incubation overnight at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... The beads were freed from storing solution by magnetic separation and then the antibody conjugation performed by adding 200µl of the Ab Binding Buffer (Thermo Fisher), and 5 µg of DCTN1 antibody ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions (5 × 105 - 2 × 106 total cells) were washed with PBS and resuspended in 1X Annexin binding buffer (Thermofisher) and washed once ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibody binding was detected by a goat-anti-rabbit antibody conjugated to horseradish peroxidase (HRP) (1:2000 dilution; ThermoFisher). Signals were detected using a chemiluminescence substrate and the Azure c600 Imaging System (Azure Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... Immediately prior to loading into the flow cytometer the supernatant was carefully aspirated and cells resuspended in 200 µL of binding buffer containing 500 nM Sytox Blue (ThermoFisher Scientific (Invitrogen)) and analyzed by BD Fortessa cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: Harvested supernatant was diluted 2× in Tris-Hcl 50 mM NaCl 150 mM CaCl2 2 mM pH 8.0 (binding buffer) and incubated with immobilized p-aminophenyl phosphoryl choline beads (ThermoFisher Scientific) at room temperature for one hour ...
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bioRxiv - Microbiology 2024Quote: ... Adjusted amounts of iRBCs were resuspended in binding medium (RPMI 1640 powder (Gibco 51800: W/L-Glutamine W/O NaHCO3) with HEPES pH 7 ...
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bioRxiv - Biophysics 2023Quote: ... the samples were added with a DNA-binding dye 4’,6-diamidino-2-phenylindole (DAPI, 1 μg mL-1 in PBS, Invitrogen) along with the secondary antibody solution ...
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bioRxiv - Immunology 2023Quote: ... Recombinant S309 Fab used for SPR binding studies was produced in ExpiCHO-S cells and purified using a Capture Select CH1-XL MiniChrom Column (ThermoFisher), followed by buffer exchange into PBS using a HiPrep 26/10 Desalting Column (Cytiva).
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bioRxiv - Biochemistry 2023Quote: ... Binding capacity Ni-beads 40 mg/ml medium) was mixed with Alexa Fluor 488-C5-maleimide (AF488, Thermo Fisher Scientific) (final concentration 10 µM ...
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bioRxiv - Cell Biology 2022Quote: ... KATNAL1 and KATNB1 and their binding partners were then co-immunoprecipitated using the Pierce Co-immunoprecipitation Kit (Thermo Scientific, 26149) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Cells were collected following transfection incubations and resuspended in Annexin V binding buffer and Annexin V AlexaFluore 350 conjugate (Thermofisher) or Propridium Iodide (PI ...
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bioRxiv - Immunology 2023Quote: Non-specific Fc binding was blocked for 10 minutes on ice with unconjugated anti-CD16/CD32 (93; Thermo-Fisher Scientific). Cells were subsequently labeled for 30 minutes on ice with the following antibodies and reagents ...
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bioRxiv - Physiology 2023Quote: ... nearly 106 cells were first incubated on ice for 30 minutes in a solution consisting of a 1:10 Fc Receptor Binding Inhibitor (eBiosciences) and 1:500 Live/Dead Aqua stain (Invitrogen) diluted in PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Cross-linked samples were divided into 12 eppendorf tubes with each tube containing 200 µl of the cross-linked sucrose gradient sample mixed with 300 µl of the lysis/binding buffer from the mirVana kit (Invitrogen), 600 µl of acid-phenol ...
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bioRxiv - Pathology 2023Quote: ... Binding of the primary antibody was detected by an enhanced chemiluminescence method (SuperSignal™ West Femto, 34094, Thermo Fisher Scientific) using horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit IgG ...
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bioRxiv - Pathology 2023Quote: ... Binding of the primary antibody was detected by an enhanced chemiluminescence method (SuperSignal™ West Femto, 34094, Thermo Fisher Scientific) using horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit IgG ...
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bioRxiv - Plant Biology 2024Quote: All fragments including the CENPC-k and CENPC motifs with and without DNA-binding regions (Supplementary Table 1-4) were amplified from the αKNL2 and CENP-C cDNA clones in pDONR221 (Invitrogen) vector using primers listed in Supplementary Table 5 ...
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bioRxiv - Microbiology 2024Quote: ... 1% hematocrit) were resuspended in binding medium (RPMI 1640 medium, 2% glucose, pH 7.2; Gibco, Thermo Fisher Scientific, Bremen, Germany) and co-incubated with HBEC-5i cells (70-90% confluence ...
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bioRxiv - Microbiology 2024Quote: ... hybridomas were screened by enzyme-linked immunosorbent assay (ELISA) using Maxisorp high binding 96 well plates (442404, Thermo Fisher Scientific) coated with 100 ng of FimH-DSG WT protein ...
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bioRxiv - Synthetic Biology 2021Quote: ... The T cells were isolated using the EasySep human T cell isolation kit (Stemcell) and activated with human T-activator CD3/CD28 Dynabeads (Gibco) at a bead:cell ratio of 1:1 ...
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bioRxiv - Microbiology 2021Quote: ... which is composed of two ACE2 ectodomains linked to the Fc portion of the human IgG (Anand et al., 2020).Alexa Fluor-647-conjugated goat anti-human Abs (Invitrogen) were used as secondary antibodies to detect ACE2-Fc and plasma binding in flow cytometry experiments.
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bioRxiv - Immunology 2022Quote: ... For Cytokine Multiplex of Human Inflammation Panel (Invitrogen™ Inflammation 20-Plex Human ProcartaPlex™ Panel) (Catalog # EXP20012185901, eBioscience/Invitrogen) procedures were performed following manufacturer protocol and the plate was processed on BioRad Bio-Plex 200 system with Bio-Plex-HTF attachment (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from human NSCLC cells and human clinical NSCLC tumor and normal samples by homogenization in Trizol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... supernatants were diluted 4-fold and assayed with the Amyloid beta 40 Human ELISA Kit and either the Amyloid beta 42 Human ELISA Kit or the Amyloid beta 42 Human ELISA Kit Ultrasensitive (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme-linked immunosorbent assays (ELISAs) for human IL-8 were performed using the Human IL-8 ELISA Kit (ThermoFisher SCIENTIFIC) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: HMF and MRC5 fibroblasts were transfected with a pool of 3 siRNAs targeting each of the 710 human kinases (Silencer Human Kinase siRNA Library, #A30079 Thermofisher). Fibroblasts (1250 cells/well ...
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bioRxiv - Immunology 2019Quote: ... human PBMC derived T cells were purified by Human T cell isolation kit from Invitrogen (Invitrogen Dynal AS, Oslo, Norway) according to manufacturer’s instruction ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were rinsed and re-suspended in flow buffer containing 25 μL mL−1 of each of the following monoclonal anti-human antibodies per sample: anti-human CD3-AF700 (56-0037-42, ThermoFisher), - CD40L/CD154-FITC (11-1548-42 ...
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bioRxiv - Immunology 2021Quote: ... and total T cells were purified from PBMC suspensions by negative immunomagnetic selection (purity >90%) using the Human Myeloid DC Enrichment Kit (STEMCELL) and the Untouched total human T cell (Invitrogen) kits ...
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bioRxiv - Cancer Biology 2022Quote: ... primary human breast CAFs were transfected with 75 nm human periostin-targeting stealth siRNA (si-POSTN) (ThermoFisher siRNA ID: HSS116400) or 75 nm non-targeting control siRNA (si-Control ...
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bioRxiv - Cell Biology 2022Quote: ... were prepared from fresh human umbilical veins as previously described (Barbieri et al., 1981) and maintained in Human Endothelial SFM (Gibco™ ...
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bioRxiv - Microbiology 2022Quote: ... Surface PCDH1 was stained using human anti-EC7 mAb-3677 (5 µg/mL) followed by anti-human Alexa FluorTM 555 (ThermoFisher) for 1 h at 4°C each ...
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bioRxiv - Microbiology 2022Quote: ... encoding full-length human MCT1 (uniprot ID: P53985) and human basigin (uniprot ID: P35613-2) were cloned individually into pFastBac vector (Invitrogen) for recombinant expression in Sf9 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... human ETAR (residues 21-406) and human ETBR (residues 27-424) DNA was cloned into a modified pFastBac vector (Invitrogen), which contains an N-terminal FLAG tag (DYKDDDD ...
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bioRxiv - Neuroscience 2023Quote: ... and secreted signaling proteins were measured in culture supernatants by the Human Immune Monitoring Center (HIMC) at Stanford University using a Human 48-plex Luminex Procarta Immuno-assay (ThermoFisher).
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bioRxiv - Neuroscience 2023Quote: ... The human FUS or human TARDBP cDNA was subcloned into the pENTR™/D-TOPO® vector (Thermo Fisher Scientific). To generate the Gateway destination vector pUAST-DEST ...