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Citations for Thermo Fisher :
4901 - 4950 of 10000+ citations for 6 AMINO 4 METHYL INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... were obtained from the Rett Syndrome Research Trust and cultured with DMEM supplemented with 15% FBS and 1x non-essential amino acids (Gibco Cat. #: 11140050). All cells were incubated in a 37°C incubator with 5% CO2.
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bioRxiv - Neuroscience 2021Quote: ... Sodium pyruvate (2 mM), MEM amino acids solution (50X, without L-Glutamine) and MEM vitamins solution (100X) were added from concentrates (Fisher Scientific, UK). The pH was adjusted to 7.5 (osmolality ~308 mmol kg-1).
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bioRxiv - Molecular Biology 2021Quote: ... and plated onto Geltrex-coated dishes with MEF conditioned medium (CM) (DMEM/F12 supplemented with 15% KSR, 100μM β-mercaptoethanol, 1% nonessential amino acid stock (all from Thermo Fisher Scientific), 10ng/mL bFGF and 10 mM Y-27632 (both from the Institute for Technical Chemistry ...
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bioRxiv - Biochemistry 2020Quote: ... medium supplemented with 10% (v/v) foetal bovine serum (FBS, Thermo Fischer Scientific) and 1% (v/v) non-essential amino acids (NEAA, Thermo Fisher Scientific). Growth medium was refreshed on average every 48 h and cells were generally split 1/10 when at 60-70% confluence.
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bioRxiv - Neuroscience 2019Quote: ... Site-directed mutagenesis of the linker sequences and residues in cpEGFP was performed using primers containing randomized NNB codons (48 codons in total, encoding all 20 amino acids; Thermo Fisher Scientific). For AAV package ...
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bioRxiv - Neuroscience 2019Quote: ... essential medium non-essential amino acids solution (1/100) and 5% (v/v) heat inactivated fetal bovine serum (Invitrogen, Paisley, Scotland, UK) and were maintained at 37°C in an atmosphere with 5% CO2 ...
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bioRxiv - Bioengineering 2021Quote: ... 15240062), non-essential amino acids (NEAA, Cat. No. 11140050), and trypsin-EDTA (0.5%, Cat. No. 2530054) were from Life Technologies (The Netherlands). FBS types were from Bovogen (Cat ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were sequenced by Edman Degradation using a Perkin Elmer 494 Procise Protein/Peptide Sequencer with an on-line 140C PTH Amino Acid Analyzer (Applied Biosystems, Inc).
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bioRxiv - Neuroscience 2020Quote: The C-terminal region of Spg11 (amino acid residues 1468-1815) was amplified using Phusion Flash High-Fidelity polymerase (Thermo Fisher F548L) and extracted from electrophoresis gel using QIAquick Gel Extraction Kit (Qiagen 28704 ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 U/ml of penicillin and 0.1 mg/ml of streptomycin (PAN-Biotech), 1x non-essential amino acid solution (from 100x stock, PAA) and 1 mM sodium pyruvate (Thermo Fisher Scientific). A549 cells (human ...
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bioRxiv - Plant Biology 2021Quote: ... The OmAGAL2 coding sequence without the expected signal peptide (SP) sequence (ΔSP-OmAGAL, amino acids [AA] 47–412) was cloned into the pENTR/D-TOPO vector (Thermo Fisher Scientific) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... were cultivated in minimum essential medium supplemented with 1x non-essential amino acid solution (from 100x stock, PAA) and 1 mM sodium pyruvate (Thermo Fisher Scientific). 293T cells that stably express ACE2 were generated by retroviral (murine leukemia virus ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (PAN-Biotech), 1x non-essential amino acid solution (from 100x stock, PAA) and 10mM sodium pyruvate (Thermo Fisher Scientific). Cell lines were incubated at 37 °C in a humidified atmosphere containing 5 % CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10% FBS, 1% pen/strep, 1x non-essential amino acid solution (from 100x stock, PAA) and 1 mM sodium pyruvate (Thermo Fisher Scientific). Cell lines were validated by STR-typing ...
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bioRxiv - Pathology 2022Quote: Plasma levels of Aspartate transaminase (AST) and alanine amino transferase (ALT) were determined spectrophotometrically using standard kits (Thermo Fisher Scientific, Waltham, MA), as described previously (13) ...
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bioRxiv - Immunology 2020Quote: ... FcMBL was biotinylated at the N terminus of the Fc protein using an N-terminal amino-oxy reaction and then coupled to streptavidin superparamagnetic beads (1µM MyOne Dynabead (Thermo Fisher Scientific, USA). Samples were screened using 5 µg of the FcMBL beads ...
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Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic developmentbioRxiv - Microbiology 2020Quote: ... The nucleoids were subsequently stained for 5 min at room temperature with 7-amino-actinomycin D (1 mg/ml 7-AAD in DMSO, Thermo Fisher Scientific) diluted 1:400 in PBS buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 U/ml of penicillin and 0.1 mg/ml of streptomycin (PAN-Biotech), 1x non-essential amino acid solution (from 100x stock, PAA) and 1 mM sodium pyruvate (Thermo Fisher Scientific). Calu-3 (ACE2 ...
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bioRxiv - Systems Biology 2019Quote: ... with 5’-amino modification and were coupled with NHS (N-hydroxysuccinimide)-ester labelled fluorescent dyes (Alexa 488, 594, 647 (Thermo Fisher Scientific) and Cy3B (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... EBV-LCLs were cultured in customized RPMI with the same formula but lacking the two amino acids tyrosine and phenylalanine (Thermo Fisher Scientific) and with dialysed FBS (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... PigMel cells were cultured at 37°C with 10% CO2 in MEM medium supplemented with 1X MEM non essential amino acids (Thermo Fisher Scientific), 10mM Na pyruvate ...
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bioRxiv - Biochemistry 2022Quote: N-terminal amino acid sequencing for the eOD-GT8 60mer was performed using the Procise Protein Sequencing System (Applied Biosystems, CA, USA). The protein band corresponding to the eOD-GT8 60mer (~50 kDa ...
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bioRxiv - Synthetic Biology 2022Quote: ... we used CF-TXTL reaction solutions with 20 amino acid mixtures containing stable isotope-labeled (heavy) L-arginine (13C6, 15N4) and L-lysine (13C6, 15N2) (Thermo Fisher Scientific) instead of unlabeled (light ...
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bioRxiv - Neuroscience 2022Quote: ... 6 well plate for 3 days with neuronal induction media (NIM: Knockout DMEM/F12, 1X N2 Supplement, 1X Non-Essential Amino Acids, 1X Glutamax (all from Thermo Fisher Scientific), 10 µM Rock inhibitor Y-27632 (SelleckChem ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... and 100 µg/ml streptomycin supplemented with 1X Non-Essential Amino Acid solution and 1 mM sodium pyruvate (Life Technologies, Gibco™). HEK293 cells were purchased from ATCC and maintained in DMEM containing 10% (v/v ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... and 100 µg/ml streptomycin supplemented with 1X Non-Essential Amino Acid solution and 1 mM sodium pyruvate (Life Technologies, Gibco™). HEK293 cells were purchased from ATCC and maintained in DMEM containing 10% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: To quantify changes in protein levels upon RPS26 silencing we applied SILAC in HEK293T cells grown in light and heavy amino acids (13C6 15N2 L-lysine-2HCl and 13C6 15N4 L-arginine-HCl) containing media (Thermo Fisher Scientific). Cells were cultured in light or heavy media for more than 14 days to ensure maximum incorporation of labeled amino acids ...
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bioRxiv - Neuroscience 2024Quote: ... Incorporated AHA was detected using Fluorescent Non-canonical Amino Acid Tagging (FUNCAT) via cycloaddition of alkyne-conjugated Alexa Fluor 647 (Thermo Scientific A10278) (Dieterich et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Cytiva HyClone™ HEPES Solution (SH3023701) and Cytiva HyClone™ Non-Essential Amino Acids 100X Solution (SH3023801) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Codon-optimized gene encoding the full-length SARS-CoV-2 PLpro (1-315 amino acids) was custom synthesized (Invitrogen, Thermo Fisher Scientific) and was cloned using XhoI and NdeI restriction sites in pET28c vector containing an N-terminal 6X histidine tag ...
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bioRxiv - Molecular Biology 2023Quote: ... Codon-optimized gene encoding the full-length SARS-CoV-2 PLpro (1-315 amino acids) was custom synthesized (Invitrogen, Thermo Fisher Scientific) and was cloned using XhoI and NdeI restriction sites in pET28c vector containing an N-terminal 6X histidine tag ...
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bioRxiv - Immunology 2024Quote: ... A polyclonal antibody against the ROTR-1 protein was raised using the peptide LDRSPPSDDGTQKV (ROTR-1 amino acids 288 to 301) in rabbit (Thermo Fisher Scientific). Anti-ROTR-1 sera was used at a dilution of 1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Nissui Pharmaceutical, Tokyo, Japan) containing 10% fetal bovine serum (FBS) and non-essential amino acids (NEAA, Thermo Fisher Scientific). Human hepatocellular carcinoma-derived HepaRG cells ...
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bioRxiv - Pathology 2023Quote: Plasma levels of Aspartate transaminase (AST) and alanine amino transferase (ALT) were determined spectrophotometrically using standard kits (Thermo Fisher Scientific, Waltham, MA), as described previously.32 Formalin-fixed ...
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bioRxiv - Developmental Biology 2023Quote: ... 20% Knockout Serum Replacement, 1% Glutamax, 1% Non-Essential Amino Acids, 0.1% ß-Mercaptoethanol, and 8 ng/mL bFGF, Thermo Fisher Scientific), and passaged weekly by manual disruption of 6-10 representative colonies ...
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bioRxiv - Microbiology 2023Quote: ... 13C-labeled positions and their 13C abundance were estimated by fragment patterns based on 13C-labeled standard of amino acids and an MS spectra library (mzCloud) (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... The H2-derived scFv was designed based on the amino acid sequences of the variable regions and synthesized by GeneArt (Thermo Fisher Scientific). The plasmid encoding H2 IgG is available from Addgene ...
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bioRxiv - Genomics 2023Quote: ... A 5’ universal sequence and a 5’ amino modifier C6 (5AmMC6, to prevent unbarcoded amplicons from being sequenced) were added to each primer (Supplementary Table S1, Thermo Fisher Scientific) for the first-round L-PCR.
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bioRxiv - Cell Biology 2024Quote: ... we used CEP135 immunofluorescent staining using a rabbit polyclonal antibody raised against the first 233 amino acids of CEP135 (24428-1-AP, Thermo Fisher Scientific). Sperm samples were visualized using a Leica SP8 confocal microscope in BrightR mode using an HC PL APO CS2 63x/1.40 OIL lens ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... mammalian expression vector with an amino-terminal protein C tag followed with a 3C protease cleavage site and transfected into Expi293 cells (Thermo Fisher Scientific) using FectoPRO (Polyplus-transfection ...
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bioRxiv - Immunology 2024Quote: ... resuspended in NK cell medium (RPMI 1640 medium containing GlutaMAX, 10% FBS, 1% P-S, 1% MEM Non-Essential Amino Acids Solution and 1% Sodium Pyruvate, both from Thermo Fisher Scientific), filtered using 70µm cell strainers and counted just before further use in flow cytometry experiments or in vitro experiments.
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bioRxiv - Cell Biology 2024Quote: ... and mGFP–STX17TM (amino acids 229–302) proteins were prepared using the Bac-to-Bac baculovirus expression system (10359016; Thermo Fisher Scientific). Bacmid DNAs were prepared using DH10Bac E ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 U/ml of penicillin and 0.1 mg/ml of streptomycin (PAN-Biotech), 1x non-essential amino acid solution (from 100x stock, PAA) and 10 mM sodium pyruvate (Thermo Fisher Scientific). Calu-3 cells (human ...
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bioRxiv - Immunology 2021Quote: ... mixed (1:1 in volume) with Fluo 4 dye (Fluo 4 Direct Calcium Assay Kit, ThermoFisher Scientific) and incubated at 37°C for 30 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Then day 4 aggregates containing hPGCLCs were transferred onto Nunc™ 4-Well Dishes (Thermo Fisher Scientific) containing 1× Protein Label solution ...
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bioRxiv - Neuroscience 2024Quote: ... the heads were dissected and fixed overnight at 4°C using 4% paraformaldehyde (Thermofisher, Cat#: J61899-AP). After several washes the heads were incubated in 20% Sucrose (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... the cells were loaded with the calcium indicator Fluo-4 (Fluo-4 NW Calcium Assay Kit, Invitrogen) and 500 nM SiR-tubulin Kit (Cytoskeleton ...
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bioRxiv - Developmental Biology 2024Quote: 4×10^4 hESCs were seeded on 0.5 μg/cm2 Vitronectin-coated 12-well plate (Gibco, A14700) in Nutristem hPSC XF medium (Biological Industries ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were fixed for 30 minutes at 4°C with 4% paraformaldehyde in PBS (Thermo Fisher Scientific), briefly washed three times ...
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bioRxiv - Genomics 2022Quote: Total RNA was extracted separately from testes (n = 4) and ovary (n = 4) tissues using TRIzol (Invitrogen). For each sample ...