Labshake search
Citations for Thermo Fisher :
4951 - 5000 of 10000+ citations for 6 AMINO 4 METHYL INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... Protein samples were separated on a 4-12% NuPAGE 4-12% Bis-Tris protein gels (Thermo Scientific) for 1 h at 170 V in 1× NuPAGE MOPS SDS running buffer (Thermo Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were loaded into 4-20% Tris-Glycine gel (Invitrogen Novex WedgeWell 4 to 20%, Tris-Glycine) and run at 150 V for 60 min ...
-
bioRxiv - Microbiology 2023Quote: ... Supernatant was mixed in a 4:1 ratio with 4-methylvaleric acid (Thermo Fisher catalogue number AAA1540506) in 6% v/v phosphoric acid and copper sulfate pentahydrate ...
-
bioRxiv - Neuroscience 2024Quote: ... the heads were dissected and fixed overnight at 4°C using 4% paraformaldehyde (Thermofisher, Cat#: J61899-AP). After several washes the heads were incubated in 20% Sucrose (Invitrogen ...
-
bioRxiv - Cancer Biology 2024Quote: ... Stroma was thoroughly minced and incubated in 4 mg/ml collagenase type 4 (Thermo Fisher Scientific; 17104019) in DMEM with 1 U/ml RNAse inhibitor for 1 h at 37°C in a rotator ...
-
bioRxiv - Neuroscience 2021Quote: ... grown to confluence and incubated with the calcium indicator Fluo 4-AM (Fluo-4 Direct assay kit, Invitrogen) for 60 min at 37 °C ...
-
bioRxiv - Microbiology 2021Quote: ... external cysteine residues were blocked with 107 μM 4-acetamino-4’-maleimidylstilbene-2,2’-disulfonic acid (AMS, Thermo Scientific) for 20 min on ice ...
-
bioRxiv - Cell Biology 2021Quote: ... pH 7.4) for 10mins and later incubated with 4 uM fluo-4/AM (1 mM, Molecular probes, #F14201) and 0.002% pluronic F-127 (Sigma ...
-
bioRxiv - Molecular Biology 2020Quote: ... or separating the pooled samples on a 4% agarose gel (E-Gel® EX Agarose Gels, 4%, Invitrogen), and gel purification of fragments larger than adapter dimers (>150bp) ...
-
bioRxiv - Neuroscience 2021Quote: ... Brain halves were either drop-fixed in phosphate buffered 4% paraformaldehyde 4°C (Thermo Fisher Scientific, Waltham, MA) for 24 hours at for immunohistochemical staining or micro-dissected (cortex ...
-
bioRxiv - Immunology 2024Quote: ... The tissues were fixed overnight at 4 °C in 4% methanol-free paraformaldehyde (ThermoFisher Scientific, cat no. U01H501), and then washed three times with 1xPBS for 30 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... 4 μl of 100 μl of sample was loaded onto 4-12% Novex Tris-glycine gel (Invitrogen, XP04120BOX). Western blotting was done using an anti-FLAG antibody ...
-
bioRxiv - Bioengineering 2023Quote: ... Fluo-4 reagent solution (prepared according to manufacturer’s instructions; Fluo-4 Direct Calcium-Assay-Kit, Thermo Fisher Scientific) was added to culture medium (50% of culture medium volume ...
-
bioRxiv - Neuroscience 2023Quote: ... heated for 2min at 85C and loaded on a TrisGlycine 4-20% SDS-PAGE gel (4-20%, Invitrogen) along with a PrecisionPlus Kaleidoscope protein ladder (BioRad 1610375) ...
-
bioRxiv - Cell Biology 2024Quote: ... After fixing for 1h at 4°C in 4% PFA (prepared in PBS; Thermo Fisher Scientific, catalog 043368.9M), embryos were rinsed with PBS and then cryoprotected in 30% sucrose in 0.1M phosphate buffer overnight at 4°C ...
-
bioRxiv - Genetics 2024Quote: ... and BSA (3%) for 4 hours at 4°C after which streptavidin-agarose UltraLink Resin (Thermo Fisher Scientific) for 1 hour at 4°C ...
-
bioRxiv - Biophysics 2021Quote: ... were grown in proliferation medium (DMEM-F12 GlutaMAX™ + 10% heat inactivated FBS + 1% non-essential amino acids +10 mM HEPES buffer + 1% Penicillin/Streptomycin; Gibco®, Invitrogen, USA). For microinjection the cells were seeded on glass bottom µ-Dishes with a cell location grid (grid-50 ...
-
bioRxiv - Biochemistry 2020Quote: ... Data Evaluation: Peaks corresponding to the calculated amino acid masses (MIM +/− H+ ± 2 mMU) were integrated using TraceFinder software (Thermo Scientific, Bremen, Germany). Alternatively ...
-
bioRxiv - Molecular Biology 2022Quote: HEK393T cells were maintained in HEK293T media (DMEM [Thermo Fisher Scientific], 15% FB Essence [Avantor], 2mM glutamine [Thermo Fisher Scientific], 1mM nonessential amino acids [Thermo Fisher Scientific] ...
-
bioRxiv - Neuroscience 2021Quote: ... syntaxin molecules were labeled at the N-terminal primary amino group (-NH2) of the proteins using fluorescent dye Alexa 488 N-hydroxysuccinimide (NHS) (ThermoFisher Scientific, Waltham, MA) in PBS at pH 6.5 overnight at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Biotin molecules were labeled at the N-terminal primary amino group (-NH2) of the bait proteins using N-hydroxysuccinimide (NHS)-biotin (ThermoFisher Scientific, Waltham, MA) in PBS at pH 6.5 ...
-
bioRxiv - Neuroscience 2021Quote: ... Dead cells were excluded from analysis by staining with LIVE/DEAD Fixable Dead Cell Stain (various amino reactive dyes; Molecular Probes, Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2021Quote: ... Media was also supplemented with 10% fetal bovine serum (FBS, HiMedia, Cat # RM9952) and with non-essential amino acids (MEM-NEAA, Gibco, Ref# 11140-050) for MCF-7 cells ...
-
bioRxiv - Immunology 2021Quote: ... L-glutamine and penicillin/streptomycin (10 μg/ml) and supplemented with MEM Non-Essential Amino Acids Solution (NEAA) (Gibco Life Technologies, Gaithersburg, Md.). To create a monolayer of polarized cells ...
-
bioRxiv - Immunology 2021Quote: ... L-glutamine and penicillin/streptomycin (10 μg/ml) and supplemented with MEM Non-Essential Amino Acids Solution (NEAA) (Gibco Life Technologies, Gaithersburg, Md.). To create a monolayer of polarized cells ...
-
bioRxiv - Cell Biology 2022Quote: ... Rat2 fibroblasts were cultured in the same medium with an added 1 % (v/v) of 100X MEM Non-Essential Amino Acids Solution (Thermo Fisher Scientific, # 11140068). Cells were incubated at 37 °C and 5 % CO2 ...
-
bioRxiv - Cell Biology 2022Quote: ... were grown in high glucose DMEM with non-essential amino acids supplemented with 10% (v/v) foetal bovine serum (Gibco, ThermoFisher Scientific, Waltham, MA), 1% (v/v ...
-
bioRxiv - Cell Biology 2024Quote: ... All tumor cell line was cultured in DMEM (30-2002, ATCC, Manassas, VA) supplemented with 100X non-essential amino acid (11140050, Gibco, ThermoFisher Scientific, Waltham, MA), 10% fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2023Quote: ... human gastric carcinoma cell line) were routinely cultured in RPMI 1640 medium buffered with 20 mM Hepes and GlutaMAX stable amino acids (Gibco, Thermo Fisher Scientific, USA). Medium was supplemented with 10% FCS (PromoCell ...
-
bioRxiv - Biophysics 2023Quote: ... was fused as its C-terminus to a 14 amino acid GS linker sequence followed by a β-thymosin–6xHIS tag and inserted into pFastBac (Thermo Fisher Scientific) behind the p10 promoter for expression in Sf9 cells ...
-
bioRxiv - Neuroscience 2023Quote: ... Each well was fed with 4 mL of Forebrain Third Media (DMEM/F12 Thermo Fisher 11320033, N2 Life Technologies 17502048, Glutamax Life Technologies 35050-061, MEM-Non Essential Amino Acids Gibco 11140050 ...
-
bioRxiv - Neuroscience 2023Quote: ... organoids were fed with Forebrain Forth Media (Neurobasal Life Technologies 21103-049, Ascorbic Acid Sigma-Aldrich A4544, Glutamax Life Technologies 35050-061, MEM-Non Essential Amino Acids Gibco 11140050 ...
-
bioRxiv - Neuroscience 2023Quote: ... half of the media was replaced with Forebrain Second Media (DMEM/F12 Thermo Fisher 11320033, N2 Life Technologies 17502048, Glutamax Life Technologies 35050-061, MEM-Non Essential Amino Acids Gibco 11140050 ...
-
bioRxiv - Microbiology 2023Quote: The wild-type ISG20 full coding sequence (amino acids 1–181) was cloned into the pPROEX HTa vector (Thermo Fisher Scientific, cat. 10711018) by standard molecular biology techniques ...
-
bioRxiv - Biochemistry 2023Quote: ... DNA fragment encoding TwCel5CBM was cloned (30 - 412 amino acid residues) into the directional champion pET151/D-TOPO™ expression vector (Invitrogen, Carlsbad, CA), which adds a cleavable N-terminal V5-6xHis-tag to the protein as per manufactures instructions.
-
bioRxiv - Bioengineering 2023Quote: ... 5 μM of both sensor domains were mixed with different concentrations of Gln or other amino acids in the Nunc Microwell 96-well Microplate (Thermo fisher, MA, US), and the mixture was incubated in the dark for 1–5 h at 22 °C ...
-
bioRxiv - Developmental Biology 2023Quote: ... YPH erg2Δ::Trp1 yeast was grown on a specific medium composed of 6.7g/liter BD Difco™ Yeast Nitrogen Base without Amino Acids (ThermoFisher Scientific, #BD 291940), 5g/liter Bacto™ Casamino Acids (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... We prepared matched amino acid-replete and amino acid-free media in the following way: Amino acid-free DMEM (US Biologicals, D9800-13) containing 10% dialyzed FBS (Life Technologies, A33820-01) served as our amino acid deprivation media and as our base media for making complete media ...
-
bioRxiv - Molecular Biology 2024Quote: ... MEM Non-Essential Amino Acids Solution (1/100) and 5% (v/v) heat inactivated Fetal Bovine Serum (FBS) (all supplements were from Invitrogen, (Paisley, Scotland, UK)) ...
-
bioRxiv - Cell Biology 2020Quote: ... 0.08 ml acetonitrile (Thermo Scientific, #A998-4), and 0.02 ml 2,4-dinitrophenylhydrazone (Beijingshiji ...
-
bioRxiv - Immunology 2021Quote: ... or 4-12% Tris Bis gel (Invitrogen), transferred onto PVDF membrane ...
-
bioRxiv - Developmental Biology 2021Quote: ... with a CytoSpin 4 (Thermo Fisher Scientific) at 1,000 rpm for 5 minutes followed by permeabilization with ice-cold methanol for 5 minutes ...
-
bioRxiv - Microbiology 2021Quote: ... 4% heat-treated foetal bovine serum (Gibco), 1x non-essential amino acids (Gibco) ...
-
bioRxiv - Cell Biology 2020Quote: Fluo-4 Calcium Imaging Kit (Invitrogen F10489) was used for cytosolic Ca2+ measurement ...
-
bioRxiv - Biochemistry 2020Quote: ... NativePAGE™ 4-16 % Bis-Tris (Invitrogen), according to the manufacturer’s instruction using Native PAGE™ 1X running buffer as anode buffer and NativePAGE™ 1X running buffer supplemented with NativePAGE™ 1X cathode buffer additive as cathode buffer (Invitrogen) ...
-
bioRxiv - Molecular Biology 2021Quote: ... supplemented with 4 mM glutamine (ThermoFisher Scientific), 1 mM pyruvate (ThermoFisher Scientific) ...
-
bioRxiv - Developmental Biology 2021Quote: ... 4 μL 0.1 M DTT (Thermo Fisher) and 2 μL SuperScript III Reverse Transcriptase (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2021Quote: ... and quantified by Qubit 4 (Thermo scientific) for validating a concentration higher than 5 μg/μl.
-
bioRxiv - Microbiology 2021Quote: ... 4 mM ATP (Thermo Fisher Scientific #R1441), and either 4 mM CaCl2 (to activate the kinase ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... 4 uL of 10X DTT (Invitrogen something), 2 uL of SUPERase-In ...