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Citations for Thermo Fisher :
4851 - 4900 of 5181 citations for S100A12 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... while the human pancreatic cancer cell line AsPC-1 (passage 11) was cultured in RPMI-1640 medium (L-glutamine supplement; Gibco, Thermo Fisher Scientific), both supplemented with 10 % (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed five times with PBS and labeled with secondary antibodies (goat anti-rabbit/human conjugated to Alexa Fluor 647; 1:1000; Invitrogen, #A21246/A21445) and FluoTag-X4 anti-GFP conjugated to Atto488 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: ... were cultured in in K-SFM + Human recombinant Epidermal Growth Factor (rEGF) and Bovine Pituitary Extract (BPE) supplemented with 1% penicillin and streptomycin (all from Thermo Fisher Scientific). PCa cell line PC-3 (CRL-1435) ...
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bioRxiv - Genetics 2023Quote: ... cells were stimulated with Dynabeads Human T-Activator αCD3/αCD28 at a bead-to-cell ratio of 1:2 (11131D, Thermo Fisher Scientific). Cell counts were determined every 2-3 days ...
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bioRxiv - Cell Biology 2023Quote: ... seeding media was exchanged for fresh media containing 10% human serum from pregnant or non-pregnant donors together with EdU (Click-iT™ EdU Proliferation Assay for Microplates, ThermoFisher Scientific) and cultured for 1 week ...
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bioRxiv - Cancer Biology 2023Quote: ... the sections were stained with an anti-human CD4 Ab that was pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes, A20187), anti-human/mouse Foxp3 Ab pre-labeled with Alexa Fluor 555 (Molecular Probes ...
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bioRxiv - Cancer Biology 2023Quote: ... FLC1025 and FLCmet3 cells were generated directly from a human FLC tumors and maintained in DMEM/F12 supplemented with 10% FBS (Thermo Fisher Scientific), 0.04 µg/mL dexamethasone (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... ATCC TIB-202) or primary human monocytes (PHMs) were propagated in RPMI 1640 with L-glutamine and 25 mM HEPES buffer (Invitrogen, Carlsbad, CA), supplemented with 10% fetal bovine serum ...
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bioRxiv - Synthetic Biology 2024Quote: ... were washed three times in cold flow buffer and then the secondary antibody (Goat anti-Human IgG H+L Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488, ThermoFisher Scientific) at a dilution of 1:500 was added ...
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bioRxiv - Developmental Biology 2024Quote: The targeted transcriptome sequencing was performed on the Ion S5XL Next Generation Sequencing system with the Ion AmpliSeq Transcriptome Human Gene Expression Assay (ThermoFisher Scientifics Inc). This assay covers 20,802 human RefSeq genes (>95% of UCSC refGene ...
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bioRxiv - Microbiology 2023Quote: ... medium was changed to differentiation medium containing a 1:1 mixture of IntestiCult™ OGM Human Basal Medium (#100-0190) and Advanced Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12, ThermoFisher Scientific, 12634-028) supplemented with 1% Pen-Strep ...
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bioRxiv - Immunology 2023Quote: ... Results of the human samples were developed using a SARS-CoV-2 convalescent plasma as primary and a goat anti-human IgG Alexa Fluor 488 secondary antibody (ThermoFisher Scientific #A48276) (2) ...
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bioRxiv - Synthetic Biology 2023Quote: Mixed CD4+ and CD8+ primary T cells were isolated with the Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Fisher) from healthy donor whole blood obtained from the Stanford Blood Center (Stanford ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and a 1:1000 dilution of goat anti-human IgG (γ-chain specific) alkaline phosphate conjugate antibody (A3187, Thermo Fisher) was added and incubated for 1 h ...
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bioRxiv - Synthetic Biology 2023Quote: ... and envelope plasmids into human embryonic kidney (HEK 293T) cells using Lipofectamine 3000 according to the manufacturer’s instructions (ThermoFisher Scientific, MA, USA). Cell supernatant containing lentivirus was collected at 24 and 48 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Human brain tissue was homogenised with a Dounce homogeniser and placed in a low protein binding 15mL tube (Thermo Fisher Scientific, 30122216) containing 10mL 1X artificial CSF (pH 7.4 ...
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bioRxiv - Microbiology 2024Quote: ... falciparum B11 line (Perrin et al., 2018) was maintained at 37°C in human RBCs in RPMI 1640 containing Albumax II (Thermo Fisher Scientific) supplemented with 2mM L-glutamine ...
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bioRxiv - Molecular Biology 2024Quote: ... The diluted pre-amplified products were then utilized directly for real-time PCR reactions according to the manufacturer’s protocol (Applied Biosystems™ TaqMan™ Array Human MicroRNA A+B Cards Set v3.0, ThermoFisher Scientific). For the data analysis ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed three times with PBS and incubated with CD15 (C3D-1) mouse anti-human monoclonal antibody (Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells stably expressing the human D1R (D1-HEK) (up to pass 35) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco/Fisher Scientific, Hampton, NH) supplemented with 10% fetal bovine serum (Omega Scientific ...
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bioRxiv - Immunology 2024Quote: ... The concentration of hIL-1β and hIL-8 were measured using a human Invitrogen ELISA Kit (Thermo Fisher Scientific, Waltham, MA, USA). All the protocols were used according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... DD5A)29 and three controls (Ctl1B, Ctl7C, Ctl8F)47 were cultured on human recombinant laminin LN521 (Biolamina) in Essential-8TM medium (Thermo Fisher Scientific) and passaged using Trypl Express (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: Cryopreserved primary human hepatocytes were genotyped for PNPLA3 rs738409(G) using TaqMan® SNP Genotyping Assays (Life Technologies, Assay ID C_7241_10, 4351379) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were transferred to gelatinized slides and immunofluorescence staining was performed using a human CD45 antibody (Rat. YAML501.4; Thermo Fisher Scientific, USA), GFP Polyclonal Antibody (A-11122 ...
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bioRxiv - Immunology 2024Quote: ... highly purified (93-98%) naïve human CD4 T cells were activated using anti-CD3+anti-CD28 Dynabeads (1:1, Thermofisher scientific, cat # 11161D) for 8-24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... one million human iPSCs were thawed and resuspended with 1 mL of Dulbecco’s Modified Eagle Medium F12 (DMEM F12) media (Thermofisher, Catalog No. 12634010) then diluted in 2 mL of warm DMEM F12 in a 15 mL Eppendorf conical tube (Eppendorf ...
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bioRxiv - Cell Biology 2024Quote: The pTP6-3xFlag-KDM6A was obtained through EcoRI digestion and subcloning into the pTP6 vector59 of the 3xFlag-KDM6A sequence optimized for human expression (purchased from Thermo Fisher Scientific). The cDNA encoding for human ZFX was purchased from Genescript ...
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bioRxiv - Cell Biology 2024Quote: Plasmids encoding human ZFP36 proteins TTP and Brf1: human ZFP36 and ZFP36L1 open reading frames were amplified from the ORFeome cDNA clone library (Invitrogen, Life Technologies) by Phusion PCR (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... human MSCs were isolated from the UC (Wharton’s Jelly) and expanded with proliferation medium (Minimum essential Medium-α (MEM-α) (Gibco™) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... Phosphorylation was induced by incubating 1 µg of recombinant human polo-like kinase 3 (PLK3) protein (Thermo Fisher Scientific, MA, USA; #PR7316B) with 50 µL of mouse WT α-synuclein in kinase reaction buffer for 8 hrs at 30°C ...
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bioRxiv - Neuroscience 2024Quote: ... The respective mouse and human brain sections were subsequently incubated with Invitrogen donkey anti-rabbit Alexa Fluor 488 (Catalog No. A-21206; Invitrogen, Carlsbad, CA) for VGLUT2 ...
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bioRxiv - Biochemistry 2024Quote: Human embryonic kidney cells (HEK293T, ATCC, #CRL-3216) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher Scientific, Invitrogen, Cat#11965092) supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2024Quote: Human embryonic kidney cells (HEK293T, ATCC, #CRL-3216) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher Scientific, Invitrogen, Cat#11965092) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... The constructs were used for in vitro transcription and translation using the 1-Step Human High-Yield Mini IVT Kit (Thermo Fisher; 88891) according to the manufacturer’s instruction ...
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bioRxiv - Pathology 2024Quote: For routine qPCR analyses 100 mg of grey matter from the STR and the CTX (Brodmann’s area 9/10) of human brains (Table 1) was dissected and extracted in 1.5 ml Trizol reagent (ThermoFisher/Life Technologies, 15596026) using a hand-held homogenizer (6 x15 s bursts)(Pro-Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Human slices were routinely stained with anti-NeuN (diluted 1:300 from stock; Rabbit anti-NeuN, Thermo Fisher Scientific PA5-37407, RRID:AB_2554049), visualized with AF488-conjugated anti-rabbit secondary antibody (1:300 from stock ...
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bioRxiv - Developmental Biology 2024Quote: ... in 2 mL complete human medium (DMEM/F12 [Gibco, Massachusetts, US], 10% FBS [PAA Cell Culture Company, UK], 1% GSP [Gibco, Massachusetts, US]). All cultures were maintained at 37 °C/5% CO2 in a humidified incubator ...
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bioRxiv - Biochemistry 2024Quote: MS/MS searches were carried out using SEQUEST search algorithms against a Uniprot database for humans (supplemented with exogenous sequence for anticipated protein constructs) using Proteome Discoverer (Version 3.0, Thermofisher Scientific, Bremen, Germany). Oxidation of methionine and N-terminal protein acetylation were included as dynamic modifications and carbamidomethylation of cysteine was set as a static modification ...
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bioRxiv - Microbiology 2024Quote: ... Upon stabilisation of baseline readings for human plasma experiments plasma was diluted 1:40 in media (RPMI [Thermo Fisher Scientific, U.S.A.] with B27 Supplement [Thermo Fisher Scientific, U.S.A.]), then transferred to the hiPSC-derived cardiomyocytes for 48 hours of treatment either in the presence or absence of 100ng/mL of dexamethasone (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: Human monocyte-derived macrophages (hMDM) were cultured in Nunc™ MicroWell™ 96-well optical-bottom plates (Thermo Fisher Scientific, Waltham, MA) and left untreated or treated with vehicle (sterile 1X PBS) ...
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bioRxiv - Bioengineering 2024Quote: ... flow throughs and washes from StEVAC method were suspended in 20 µl of Exosome-human CD81 flow detection beads (Thermo Scientific, 10622D) and incubated at 4°C overnight while rotating ...
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bioRxiv - Immunology 2024Quote: ... we co-transfected 3×106 3-day activated CD8+T cells with 150 pmol/106 cells of human TSP-4-specific siRNAs (#s14100, Thermo Fisher Scientific) and 1.5 μg/106 of the pMax-TSP-1-GFPSpark construct ...
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bioRxiv - Cancer Biology 2024Quote: ... One to four thousand AT2 cells were resuspended in 50 µl complete human alveolar organoid media [Advanced DMEM (Thermo Fisher Scientific, #12491015), 1% GlutaMax (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: SNU1967 cells were transfected with ON-TARGETplus Human siRNA SMARTpool targeting SOX9 (L-021507-00-0005) using Lipofectamine RNAiMAX (Thermo Fisher Scientific). Post-transfection ...
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bioRxiv - Synthetic Biology 2024Quote: Total secreted HSA concentrations of 72 hr yeast HSA expression cultures were quantified via commercially available Albumin Human ELISA Kit (ThermoFisher Scientific #EHALB), following the included protocol ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with Fixable Viability Dye eFluor 780 and the following monoclonal antibodies: TexasRed-PE conjugated anti-human CD3 (clone 7D6, Invitrogen, cat. MHCD0317); PerCP/Cyanine5.5 conjugated anti-human CD4 (clone OKT4 ...
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bioRxiv - Microbiology 2024Quote: Supernatant collected from human tissues at 48 or 72 hpi after infection with RVFV ZH501 or LAVs (ΔNSs, ΔNSm, MP-12, ΔNSs/ΔNSm) was used to detect total human interferon alpha (IFNα; Invitrogen catalog #BMS216) and human interleukin 29 (IL-29/IFN-λ1 ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... CR3022 antibody (10 μg/mL) and 10 μg/mL of goat anti-human IgG (H+L) secondary antibody Alexa Fluor 647 (Invitrogen #A-21445) was pre-incubated for 30 min at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... and further subcloned into pAAV2 under the control of the human synapsin promoter and flanked by lox71 and lox66 sites using EcoRI and BamHI restriction sites (FastDigest, Thermo Fisher Scientific). The constructs were soma-targeted using the Kv2.1 targeting domain.