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Citations for Thermo Fisher :
4851 - 4900 of 10000+ citations for Mouse IL 17D ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... donkey anti-mouse IgG, 1:500, 715-165-150, Jackson ImmunoResearch; AF488: donkey anti-mouse IgG, 1:500, A-21202, Life Technologies).
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bioRxiv - Molecular Biology 2024Quote: ... AlexaFluor 488 goat anti-rabbit IgG and AlexaFluor 546 goat anti-mouse IgG or Goat Anti-Mouse IgG (H + L) DyLight 680 Conjugated (Invitrogen REF 35518), Goat anti-Rabbit IgG (H&L ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers were fixed and stained at 24 hpi with mouse monoclonal antibody 1E12 followed by anti-mouse Alexa Fluor 488 (Thermo Fisher Scientific) and Hoechst nuclear stain ...
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bioRxiv - Immunology 2023Quote: ... and stained with a mouse serum that recognizes A27 (1:200 dilution) followed by adding anti-mouse AF488 (Invitrogen, A55058, 1:1000) as secondary antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... The secondary antibodies used were goat anti-mouse IgG2b 594 and goat anti-mouse IgG1 647 (all from Molecular Probes, 1:500). After several washes in PBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... and then incubated with the indicated primary antibodies (mouse anti- MIC60 [110329; Abcam], rabbit anti-MIC27 [PA5-51427; Thermo Fisher Scientific], mouse anti- TIMM23 [BDB611222; Thermo Fisher Scientific] ...
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bioRxiv - Immunology 2024Quote: Mouse lung homogenates were collected for Luminex analysis using the Cytokine & Chemokine 36-Plex Mouse ProcartaPlexTM Panel 1A (ThermoFisher, # EPX360-26092-901). Samples were incubated with fluorescent-coded magnetic beads pre-coated with respective antibodies in a black 96-well clear-bottom plate overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... abdominal carcasses were incubated in AlexaFluor conjugated secondary antibodies (goat anti-mouse 488, goat anti-mouse 568, or goat anti-rat 488, ThermoFisher; 1:250) for two hours at room temperature and protected from light.
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bioRxiv - Genetics 2024Quote: ... followed by transfected with plasmids expressing mCherry-tagged mouse DNAH12 and GFP-tagged mouse DNALI1 using Lipofectamine 3000 (Thermo Fisher, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... gout anti-mouse IgG1 Alexa Fluor 568, A-21124 and goat anti-mouse IgG2a Alexa Fluor 488, A-21131, Thermo Fisher Sci) at room temperature (23 °C ...
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bioRxiv - Neuroscience 2021Quote: ... anti-rabbit HRP antibody (1:5000) or with anti-goat HRP antibody (1:5000) was performed for 1 h and ECL (Pierce, Thermo Scientific, Rockford, IL, USA) visualized detection ...
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bioRxiv - Bioengineering 2019Quote: ... for 30 minutes non-shaking to allow for infection and then grown for 3 hours at 37°C and 225 rpm for amplification (MaxQ 4000, ThermoFisher Scientific, Hannover Park, IL). After ...
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bioRxiv - Pathology 2020Quote: ... Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis of interleukin (IL)-6 was performed using TaqMan Gene Expression Assays (Rn01410330_m1; Applied Biosystems, Foster City, CA, USA) and 7900HT Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2019Quote: 3 × 104 A4 and untransfected SH-SY5Y cells were seeded into 8-well Lab-Tek®II Chamber Slides (Nalge Nunc, Naperville, IL, U.S.A) and left for 48 h to strongly adhere ...
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bioRxiv - Molecular Biology 2021Quote: ... The bound antibodies were detected by incubation with Alexa Fluor 488-conjugated donkey anti-rabbit IgG (Thermo Fisher Scientific, Rockford, IL, USA; 1:300) or Alexa Fluor 546-conjugated donkey anti-goat IgG (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... A total of 100 μg protein from each condition was subjected to in-solution trypsin digestion before labelling the resultant tryptic peptides using the TMT-6plex Isobaric Label Reagent Set (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... The SKBR3 cell-membrane proteins were isolated by either the biotinylation of amino (EZ-Link Sulfo-NHS-SS-Biotin, Thermo Fisher Scientific, Rockford, IL) or oxidized glycan (EZ-Link Alkoxamine-PEG4-Biotin ...
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bioRxiv - Microbiology 2022Quote: ... Thin sections were cut with a Leica UC7 ultramicrotome (Buffalo Grove,IL) prior to viewing at 120 kV on a FEI BT Tecnai transmission electron microscope (Thermo fisher/FEI, Hillsboro, OR). Digital images were acquired with a Gatan Rio camera (Gatan ...
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bioRxiv - Immunology 2022Quote: ... Macrophages were cultured in complete RPMI media (cRMPI; RPMI 1650, L-glutamine, 10% heat-inactivated fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Rockford, IL), 1% penicillin/streptomycin ...
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bioRxiv - Developmental Biology 2019Quote: ... The silicon device surfaces were functionalized with a previously described protocol using 3-mercaptopropyltrimethoxysilane (MPTMS) and N-gamma-maleimidobutyryloxysuccinimide ester (GMBS) NeutrAvidin-biotin chemistry (Thermo Fisher Scientific, Rockford, IL)[32 ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with rabbit antibodies against SFTSV-N as primary antibodies and HRP-conjugated recombinant protein A/G (Cat. No. 32490, Thermo Scientific, Rockford, IL, USA) as secondary antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated at 37 °C for 7 days with secondary Alexa Fluor 647 donkey anti-rabbit IgG (1:450; Thermo Fisher Scientific, Rockford, IL) in 3% donkey serum/PTwH and then washed in PTwH for 5 times.
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bioRxiv - Immunology 2020Quote: ... Monocytes were differentiated with GM-CSF (800U/ml) and IL-4 (500U/ml) in enriched RPMI (Gibco, 10% bovine serum, P/S, 2mM Glutamine) in a 5% CO2 humidified 37°C incubator as previously described (Geijtenbeek et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Myofibrillar proteins were pelleted by centrifugation at 700g for 5min and the protein concentration of the sarcoplasmic fraction (supernatant) was determined via a bicinchoninic acid assay (Thermo Fisher Scientific, Rockford, IL). Samples were then diluted to equal concentrations in 1X Laemmli sample buffer and denatured at 95℃ for 5min ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were incubated for 12 to 15 hours and then washed with 2 mL of a 0.25 % trypsin solution (25200056, Gibco, Fisher Scientific, Hanover Park, IL, USA) in 1X EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... The slides were incubated in a humidity controlled (50%) enclosure for 45 minutes and blocked by incubation with Superblock(tm) blocking buffer (Thermo Fisher Scientific, Rockford, IL) for 45 minutes at room temperature with shaking at 60rpm.
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bioRxiv - Developmental Biology 2021Quote: ... and centrifuged at 13000×g for 20 min at 4°C.The protein concentrate on of collected supernatant was determined using bicinchoninic acid (BCA) assay (23225; Thermo Fisher Scientific, Rockford, IL, USA). An equal amount of each protein sample was electrophoresed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Immunology 2021Quote: ... IL-1β and TNF-α – levels in cell culture supernatants were used (#88-7066, #88-7106, #88-7261, #88-7346, Thermo Fisher Scientific, USA). ELISA kits are based on the sandwich immunoassay technique ...
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bioRxiv - Immunology 2022Quote: ... Pen/Strep (Biological Industries, Israel) and 3,000 IU/ml IL-2 (Chiron Novartis, New Jersey, USA) in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA). Cells were split or medium was added every 2 to 3 days ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... The membranes were washed with TBST (thrice for 5 minutes each) and developed using Supersignal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, IL, USA). The bands were visualized through ImageQuant LAS500 series (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h RT and incubated o/n with a primary antibody cocktail containing anti-GFP antibody (polyclonal chicken anti-GFP, 1:2000; #A10262, AB_2534023; Thermo Fisher Scientific, Rockford, IL, USA), anti-TH antibody (TH polyclonal rabbit anti-mouse ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed once with PBS containing both calcium and magnesium and labeled with 0.5 mg of membrane-impermeable EZ-linkTM Sulfo-NHS-Biotin (Thermo Fisher Scientific, Rockford, IL, USA) per plate for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The carbohydrate and protein levels in the food waste were quantified using the anthrone method [32] and the modified Lowry method (Thermo Fisher Scientific, IL, USA), respectively ...
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Dynamic Effects of Ventral Hippocampal NRG3/ERBB4 Signaling on Nicotine Withdrawal-Induced ResponsesbioRxiv - Neuroscience 2023Quote: ... frozen VH tissue was weighed and gently homogenized with a glass dounce tissue grinder in 10 vol (1:10, wt/vol) of Syn-PER synaptic protein extraction reagent (Thermo Fisher Scientific, Rockford, IL) supplemented with a protease and phosphatase inhibitor cocktail ...
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bioRxiv - Cancer Biology 2023Quote: ... The total proteins were in the supernatant and normalized to 1mg/mL based on the Micro BCA assay according to manufacturer’s recommendations (Thermo Fisher Scientific Inc, Rockford, IL). 25 μL of each sample was diluted in the Diluent Buffer to 100 μL and added in the EGFR capture plate ...
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bioRxiv - Microbiology 2023Quote: ... 60 L of seawater were collected from 5 m depth in the center of the Gulf of Trieste and aged in acid-washed and Milli-Q water-rinsed 20 L Nalgene carboys (ThermoFisher Scientific, Rockford, IL, USA) for about one month at room temperature in the dark ...
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bioRxiv - Immunology 2023Quote: 1 × 106 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 or PBS for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were spun down and washed once with PBS before being flash frozen in liquid nitrogen ...
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bioRxiv - Immunology 2023Quote: 5×105 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were spun down and washed once with PBS before total RNA was isolated from cells by Purelink™ RNA Mini Kit (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were stimulated with IL-4 (20ng/mL) for 24 hours before lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). 75,000 DiO (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were stimulated with IL-4 (20ng/mL) for 24 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were diluted to a concentration of 1 million cells/mL and 50uL of cell solution was plated on a precoated 4ug/mL fibrinogen and 0.5% polyvinyl alcohol (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were treated with 20ng/mL IL-4 for 5 hours before cells were detached using PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were then stained with a combination of Mitotracker Green FM ...
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bioRxiv - Plant Biology 2023Quote: ... The quantity and quality of DNA were confirmed by electrophoresis on agarose gels stained with GelRed and visualized under UV light using a NanoDrop 1000 spectrophotometer (Thermo Scientific, Rockford, IL, USA). Nine samples (three from each treatment ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with Alexa Fluor-conjugated secondary antibodies (fluorescence at 488, 594, 555, or 645 nm; Thermo Fisher Scientific, Rockford, IL, USA). Nuclei were counterstained with DAPI (1:10,000 in PBS ...
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bioRxiv - Immunology 2023Quote: ... or IL-2 combined with spike protein for 16 hrs and used for RNA extraction using TRIzol™ reagent (Thermo Fisher Scientific, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The RdRp P/L complexes were purified using Ni-NTA affinity chromatography based on their respective eight-histidine tag as shown in Table 1 (Thermo Scientific, Rockford, IL, USA). The pET-15b (Novagen ...
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bioRxiv - Cell Biology 2024Quote: Organoids were treated from day 4 through day 11 for EN1 and day 7 through day 11 for EPC2 with or without 10 ng/mL recombinant IL-13 (200-13, Thermo Fisher Scientific, Waltham, MA), reconstituted in phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse monoclonal V5 (1:5000, Thermo Fisher Scientific, R960CUS), mouse monoclonal HDAC1 (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... and Alexa 598 goat anti-mouse/rabbit (Molecular Probes) were used as secondary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... AlexaFluor 488 conjugated to donkey anti-mouse (Life Technologies), and AlexaFluor 568 conjugated to donkey anti-mouse (Life Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-ERK 1:100 (Invitrogen, 14-9108-80), rabbit anti-pERK 1:500 (Invitrogen ...