Labshake search
Citations for Thermo Fisher :
4751 - 4800 of 10000+ citations for Mouse IL 17D ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... sections were incubated overnight at 4 °C with ~100ul primary antibodies diluted in 1% BSA+Triton-X (Thermo Fisher Scientific, Rockford, IL, USA) [1] ...
-
bioRxiv - Microbiology 2022Quote: Primary human CD4+ T cells were incubated with 20U/ml interleukin-2 (IL-2) alone or in combination with α-CD3/CD28 antibody-coated beads (100 μL/30 x 106 cells) (Gibco/Thermo Fisher) for 72 hours prior to infection with 100ng of p24 equivalent of pseudotyped pMorpheus-V5 ...
-
bioRxiv - Molecular Biology 2022Quote: ... total protein was measured from SEC fractions using a bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, IL) with an automated KoneLab instrument (Thermo Scientific, Vantaa, Finland). Based on total protein ...
-
bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitated protein was processed for western blotting as previously published (24) and developed with Clean-blot™ IP detection reagent (HRP) (Thermo Scientific, IL). Antibodies used for western blotting are listed in supplementary table S7.
-
bioRxiv - Neuroscience 2021Quote: ... 0.5% sodium deoxycholate and 0.1% SDS plus protease and phosphatase inhibitor cocktails (Cat. Nos. 78430 and 78428, respectively; Thermo Fisher Scientific, Rockford, IL). Tissue homogenates were sonicated and centrifuged as described in detail in our previous reports (13 ...
-
bioRxiv - Molecular Biology 2020Quote: ... were analyzed on a high resolution Q Exactive HF Hybrid Quadrupole - Orbitrap Mass Spectrometer equipped with a heated electrospray ionization (HESI)-II source (Thermo Scientific, Rockford, IL) and a Vanquish Horizon UHPLC system (Thermo Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... Ten- to eleven-week-old mice were anesthetized with a mixture of ketamine (Zoetis, Kalamazoo, MI) and xylazine (Akorn Animal Health, Lake Forest, IL) and inoculated intranasally with either PBS (Gibco, Grand Island, NY), 1×104 PFU ...
-
bioRxiv - Developmental Biology 2022Quote: ... Embryos were cultured at density of 10 embryos in 10 µl KSOM medium supplemented with 3 mg bovine serum albumin/mL (14) in 60-well plates (LUX 5260, Nunc, Naperville, IL, USA) overlaid with 2 mm of heavy paraffin oil (Sigma ...
-
bioRxiv - Immunology 2022Quote: ... The proteins were resolved by SDS-PAGE on 4-12% NuPAGE gels using NuPAGE MOPS buffer (NuPage gels, Thermo Fisher Scientific, Rockford, IL), and transferred to methanol-activated PVDF membrane (Perkin Elmer ...
-
bioRxiv - Cell Biology 2022Quote: ... the concentration of total soluble proteins of each sample was determined using BCA™ Protein Assay (Thermo Scientific™ Pierce™, Rockland, IL). To run a SDS gel ...
-
bioRxiv - Immunology 2022Quote: ... washed and added at 103 cells per well to various ratios of effector T cells (CTC or UTC population after overnight incubation with IL-15) in 96 well V-bottomed plates (NUNC, Thermo Fisher Scientific). After 4 hours of co-incubation ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells cultured in 24-well plates were washed with phosphate-buffered saline and treated with 0.2 ml of Mammalian Protein Extraction Reagent (Thermo Scientific, Rockford, IL, USA). An aliquot of the solubilized cell solution (4 μl ...
-
bioRxiv - Cancer Biology 2019Quote: ... Three 15 amino acid peptides (P1, P3 and P4) and one 14 amino acid peptide (P2) were synthesized (Thermo Fisher Scientific, Rockford, IL). The purity of the peptides was >95% and each peptide was modified by N-terminal acetylation and C-terminal amidation to enhance their stability.
-
bioRxiv - Physiology 2019Quote: ... The final RNA yield and purity were evaluated by the absorption ratio A260/A280 measured with a Nanodrop (Thermo Scientific, Rockford, IL, USA). The synthesis of cDNA for reverse transcription was carried out with a Protoscript II first strand cDNA synthesis kit (New England Biolabs ...
-
bioRxiv - Cell Biology 2019Quote: ... 1μg of RNA was reverse transcribed using oligo-DT and SuperScript III First-Strand Synthesis System (18080051, Thermo Fisher Scientific, Rockford, IL, USA). Relative qRT-PCR was performed in a total volume of 25 µl with 12.5 µl SYBER GREEN master mix (1725121 ...
-
bioRxiv - Neuroscience 2019Quote: ... the same protocol to collect the hemolymph was performed and measurements were done in the concavity made in the parafilm that contains the hemolymph drop with a microelectrode (Orion 9810BN, Thermo Scientific, IL, USA). We repeated the protocol in 20 different samples.
-
bioRxiv - Immunology 2019Quote: ... Hemocytes nuclei were then stained by incubating with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Thermo Fisher Scientific, Rockford, IL, USA) dissolved in PBS and washed thrice with PBS ...
-
bioRxiv - Immunology 2019Quote: ... The selected combinations of hrproteins or peptide demonstrated the highest reduction in HIV-1 p24 release without inducing tissue toxicity as indicated with a LDH assay (Pierce LDH Cytotoxicity Assay, Thermo Scientific, Rockford, IL).
-
bioRxiv - Physiology 2021Quote: ... and the powder demineralized with a 0.5M EDTA solution at pH 8.0 containing protease inhibitors (Pierce Protease Inhibitor Tablets, EDTA-free, Thermo Fisher Scientific, Rockford, IL, USA) for 12 days under agitation at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Fibroblasts cultures were culture under standard conditions in MEM medium supplemented with 10% fetal bovine serum (Thermo Scientific, Rockford IL, USA, Ref. 12657) and 1% penicillin/streptomycin (Lonza ...
-
bioRxiv - Biochemistry 2021Quote: ... HCV nsp5b RdRp was purified using Ni-NTA affinity chromatography based on the C-terminal eight-histidine tag according to the manufacturer’s specifications (Thermo Scientific, Rockford, IL, USA). NiV RdRp P/L complex coding sequence (AEZ01385 and AEZ01390 ...
-
bioRxiv - Biochemistry 2021Quote: ... NiV RdRp P/L complex was purified using Ni-NTA affinity chromatography based on the P protein N-terminal eight-histidine tag according to the manufacturer’s specifications (Thermo Scientific, Rockford, IL, USA). The protein identities of the purified HCV nsp5b RdRp and NiV RdRp P/L complex were confirmed by mass spectrometry (MS ...
-
bioRxiv - Developmental Biology 2021Quote: ... Mandibular mesenchyme (i.e., cell number per mandibular primordium) was counted using a hemacytometer (1492, Hausser Scientific Partnership, Fisher Scientific, Hanover Park, IL, USA) in combination with MIPAR software (Tyler and Hall ...
-
bioRxiv - Developmental Biology 2021Quote: Full-length cDNA synthesis from RNA was performed using Maxima H Minus reverse transcriptase (K1651, Thermo Scientific, Fisher Scientific, Hanover Park, IL, USA) following the manufacturer’s directions with 2 µg of total RNA and 100 pmol of d(T)20 VN primer ...
-
bioRxiv - Microbiology 2020Quote: ... and cultured in Eagle’s medium (MEM, Fujifilm Woko Chemicals, Japan) supplemented with 5% heat-inactivated fetal bovine serum (FBS, Thermo Fisher Scientific, Rockford, IL), 1% (w/v ...
-
bioRxiv - Microbiology 2019Quote: ... Lysozyme hydrochloride, from egg white (Wako Pure Chemical Industries, Osaka, Japan) was labeled with DyLight 488 NHS Ester (Thermo Fisher Scientific, Rockford, IL), according to the instruction.
-
bioRxiv - Immunology 2021Quote: Expanded Tregs or Tconvs were activated with IL-2 (100 IU/mL) and 1:1 anti-CD3/anti-CD28-coated beads (Gibco Dynabeads T-Expander) for 4 d ...
-
bioRxiv - Immunology 2021Quote: ... Surface-labeled cells were then washed with 1x perm-wash buffer and labeled with anti-IL-2-PE-Cy7 (Clone MQ1-17H12, Invitrogen 25-7029-42), anti-TNFα-PE (Clone Mab11 ...
-
bioRxiv - Immunology 2020Quote: Cells were plated and allowed to adhere to the cover glass of a 4-well or a 1-well chamber (Nalge Nunc International, Naperville, IL) precoated with Fibronectin (Sigma Aldrich ...
-
bioRxiv - Immunology 2022Quote: ... CD4+CD25- T cells were stimulated with anti-CD3/anti-CD28 as described above in the presence of 20 ng/ml IL-2 (eBioscience, ThermoFisher Scientific, Langenselbold, Germany) and 5 ng/ml TGF-β1 (R&D Systems ...
-
bioRxiv - Cell Biology 2022Quote: ... and lung cryosections after blocking were stained with a 1:200 dilution of Alexa Fluor 488 Phalloidin (Thermo Fisher Scientific, Rockford, IL, USA) for 1 h ...
-
bioRxiv - Immunology 2022Quote: ... Pen/Strep (Biological Industries, Israel) and 3,000 IU/ml IL-2 (Chiron Novartis, New Jersey, USA) in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA). Cells were split or medium was added every 2 to 3 days ...
-
bioRxiv - Molecular Biology 2022Quote: ... the membrane was exposed to an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) (PierceTM ECL Western; Thermo Scientific. Rockford, IL, USA). Bands were visualized and quantitated using the Chemi DocTM MP imaging system (Bio-Rad ...
-
bioRxiv - Neuroscience 2022Quote: ... the tissue was dissected and homogenized using a modified Pierce RIPA lysis and extraction buffer (RIPA+) (catalog#89900, Thermo Scientific, Rockford, IL, USA) that contains a Halt Protease Inhibitor Cocktail (catalog#1861281 ...
-
bioRxiv - Microbiology 2024Quote: ... Hyphae that were analyzed with the Lionheart FX automated microscope were inoculated in 96-well plates (#167008; Thermo Fisher Scientific; Rockford, IL, US) in 200 µl medium per well ...
-
bioRxiv - Molecular Biology 2024Quote: ... Total protein content was determined using the bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, IL) with an automated KoneLab instrument (Thermo Scientific, Vantaa, Finland).
-
bioRxiv - Genomics 2024Quote: ... Thin sections were cut with a Leica UC7 ultramicrotome (Buffalo Grove, IL) prior to viewing at 120 kV on a FEI BT Tecnai transmission electron microscope (ThermoFisher/FEI, Hillsboro, OR). Digital images were acquired with a Gatan Rio camera (Gatan ...
-
bioRxiv - Pathology 2023Quote: ... OCT embedded tissues were sectioned at 10 μm thickness and glycol methacrylate embedded at 3 μm and transferred to positively charged glass slides (Superfrost glass slides, Thermo Fisher, IL, USA). Hematoxylin/eosin (H&E ...
-
bioRxiv - Cancer Biology 2022Quote: ... spun in a microfuge at 13,000 rpm for 20 minutes at 4°C and supernatant protein concentration determined using the BCA (Thermo Fisher Scientific, Rockford, IL) or Bradford (Bio-Rad Laboratories ...
-
bioRxiv - Neuroscience 2023Quote: ... 20,000 single iPSCs were seeded onto each well of a U-botton 96-well plate (NucleonTM SpheraTM, Thermo Fisher Scientific, Rockford, IL, USA) to form embroid bodies with mTesR plus medium and 10μM ROCK inhibitor Y-27632 (Tocris Bioscience ...
-
bioRxiv - Immunology 2023Quote: ... suspended in 100 mL of sodium carbonate buffer (pH 9.6) was used to coat each well of a 96-well plate (Nalge Nunc Inc., Naperville, IL, USA) that was incubated overnight at 4 °C ...
-
bioRxiv - Physiology 2023Quote: ... Protein concentration was determined by the bicinchoninic acid protein assay (Smith et al., 1985) using bovine serum albumin as the standard (Pierce, ThermoFisher, Rockford, IL, USA).
-
bioRxiv - Cancer Biology 2023Quote: MDA-PCa-2a was cultured in BRFF-HPC1 media (AthenaES, Baltimore, MD) with 20% fetal bovine serum (FBS, Hyclone Labs, Thermo Scientific, Rockford, IL) and 0.1% penicillin-streptomycin (Hyclone Labs ...
-
bioRxiv - Genetics 2023Quote: ... Separated proteins were transferred onto a nitrocellulose membrane using an iBlot™2 Gel transfer device (cat. IB21001, Thermo Fisher Scientific, IL, USA). Non-specific protein binding to the membranes was blocked with 5% non-fat dry milk (cat ...
-
bioRxiv - Microbiology 2023Quote: ... After lysis in breaking buffer (20 mM potassium phosphate, 5 mM EDTA, 1 mg mL−1 lysozyme, 1 Pierce Protease Inhibitor Tablet (Thermo Scientific, Rockford, IL), 1 mM DTT ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with PBS followed by incubation with goat anti-rabbit IgG (H+L) Alexa fluor-594 secondary antibody (A-11012 from Thermo Scientific (Rockford, IL) was used for EphA2 ...
-
bioRxiv - Immunology 2023Quote: ... fresh media was supplemented with 30 U/mL of IL-2 and a 1:1 ratio of anti-CD3 and -CD28 Dynabeads (Thermo Fisher Cat# 11131D) and similarly treated with aphidicolin ...
-
bioRxiv - Immunology 2023Quote: Ex vivo isolated and rested CD8+ T cells or transduced T cells from splenocytes were incubated with R10 (RPMI 1640 + 10% FBS + 1% penicillin/streptomycin + L-glutamine + 20 U/ml of IL-2; Gibco, Thermo Fisher Scientific) alone or R10 containing PMA/ionomycin for two hours ...
-
bioRxiv - Biochemistry 2024Quote: ... The resulting peptides obtained from soluble or membrane protein fractions were purified by C18 solid phase extraction using the C18 Spin column (Pierce Biotechnology, Thermo Scientific, Rockford, IL) and per the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2024Quote: ... samples were clarified by centrifugation at 13.000 rpm for 15 min at 4 °C and protein concentration was determined using the BCA assay (Thermo Scientific, Rockford, IL, USA). Equal amounts of protein (40 – 50 μg ...