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Citations for Thermo Fisher :
4851 - 4900 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and were seeded at a density of 1 x 104 cells/well (200 µl culture medium per well) into Nunclon™ Sphera™ 96-well -low attachment plates (Thermo Fisher Scientific) respectively ...
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bioRxiv - Bioengineering 2023Quote: After diluting cells from culture or colonies (see above) 1:10 into PBS+0.1% Tween (total volume=200 μL) in 96-well round-bottom plates (Fisher Scientific cat #07-200-760), samples were measured for cellular composition using a ThermoFisher Attune NxT V6 Flow Cytometer at UW-Madison’s Carbone Cancer Center ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded at a density of 1×104 cells/well (200 µl of culture medium per well) into Nunclon™ Sphera™ 96-well low attachment plates (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of RNA were reverse transcribed and amplified using TaqMan™ RNA-to-C T ™ 1-Step Kit kit on the ViiA 7 Real-Time PCR System (Applied Biosystems). TaqMan gene expression assay probes (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... optical densities were determined in 200 µl culture in microtiter plates by spectrophotometry in a plate reader (Spectramax M5, Applied Biosystems, United States) and 20 µl of culture were transferred into 4 ml of fresh MMAB-medium ...
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bioRxiv - Cancer Biology 2020Quote: ... The specimen itself was floated on the water surface inside a standard multi well plate (Thermo Scientific Nunclon Delta Surface 96 well plates). Those are needed to be tape sealed in order to prevent water creep under ultrasound irradiation ...
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bioRxiv - Biochemistry 2022Quote: ... OD600 and fluorescence were measured on a Biotek Synergy H1 MF plate reader using clear-bottom 96-well plates (Thermo Scientific Nunc, #265301). Prior to plate reader measurements ...
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bioRxiv - Neuroscience 2020Quote: ... or NAD standard were combined with 50 μl of reagent in a 96-well plate (Nunc™ F96 MicroWell™ White Polystyrene plate, ThermoFisher Scientific). Luminescence was recorded at approx ...
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bioRxiv - Synthetic Biology 2019Quote: ... after which the lysates with the beads were transferred to a 96-well filter plate (Thermo Scientific, USA, Nunc 96-well filter plates), placed over a 96-well collection plate ...
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bioRxiv - Synthetic Biology 2019Quote: ... after which the lysates with the beads were transferred to a 96-well filter plate (Thermo Scientific, USA, Nunc 96-well filter plates), placed over a 96-well collection plate ...
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bioRxiv - Molecular Biology 2023Quote: ... The reactions were loaded on cellulose TLC plates (20L×L20Lcm, EMD Millipore™ Precoated TLC and PLC Glass Plates, Cat#: M1057160001, ThermoFisher Scientific) and developed in two solvent systems ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... The lysate was transferred to a clear flat-bottom 96-well plate to measure absorbance at 595 nm using a Varioskan plate reader (Thermo Scientific, Cat#: VL0000D0) and SkanIt Software for Microplate Readers ...
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bioRxiv - Microbiology 2023Quote: ... coli phenotypic morphology (pink, round) was picked from each plate and streaked for isolation onto blood agar plates (Thermo Fisher Scientific, Lenexa, KS) which were incubated at 35°C for 18-24h ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 μl forward primers (5 μM) and 0.2 μl reverse primers (5 μM) in 96-well plates (MicroAmp Fast 96-well Reaction Plate, Applied Biosystems for Life Technologies). The reactions were performed in triplicates on a QuantStudio 5 Real-Time PCR System (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2024Quote: ... MIC values were measured every 3 to 4 days in 96-well plates in plate readers (Thermo Scientific Multiskan FC with SkanIt software) according to previous description (48) ...
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bioRxiv - Biophysics 2021Quote: ... a stack of z-scan images over an axial range of 7 μm in steps of 100 nm of immobilized tetra-spectral fluorescent beads was recorded (TetraSpeck™ carboxylate micro-spheres, 0.1 μm, T7279, Thermo Fisher Scientific). Fig ...
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bioRxiv - Cell Biology 2020Quote: Intracellular pH (pHi) measurements were performed by monitoring intracellular free H+ concentration using the pH-sensitive fluorescent dye BCECF-AM (B1170; Thermo Fisher Scientific) as described previously.19,20 Details of the experimental setup and solutions used are provided in the Supplementary Materials.
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... in PBS at 200ng/ul was microinjected directly into the cytoplasm together with fluorescent reporter Dextran Texas Red (MW 70000 Da) (D1864, Invitrogen, Molecular Probes) that facilitates identification of successfully injected neurons ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were washed 3 times with PBS and were mounted on the slides using Fluorescent-G mounting medium (Invitrogen, Carlsbad, CA). Final concentrations of primary Abs were used as follows ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: ... from the pENTR clones to the BiFC vector pDEST-VYCE(R)GW carrying the C-terminal region of the Venus Fluorescent Protein (VFP) (Gehl et al., 2009) by Gateway® cloning (Invitrogen, California) from the pENTR clones to yield the constructs as listed in Supplementary table S2 ...
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bioRxiv - Cell Biology 2022Quote: ... fluorescent transgenic or mRNA injected embryos (injection protocols in [33]) were mounted in 2% low melting agarose gel (Thermo Fisher Scientific; 16520100) at 512-cell stage and imaged using SP8 LSCM or spinning disk confocal microscopes.
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bioRxiv - Genomics 2019Quote: ... The DNA purity was determined by 0.8% agarose gel 100 V electrophoresis for 40 min and DNA concentration was determined by Qubit® 2.0 fluorescent meter (Invitrogen, Carlsbad, USA). Following quality assessment ...
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bioRxiv - Cancer Biology 2020Quote: ... SALE-Cas9 and SALE-Cas9 RIT1M90I cells were transduced with YAP5SA lentivirus and 48 hours post-transduction GFP-expression was confirmed by direct fluorescent expression using the EVOS FL Color imaging system (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... HCLE cells were cultured to confluence on glass bottom dishes and pre-loaded with 5 μM Fluo-3AM fluorescent dye (Invitrogen, Carlsbad, CA) to allow for Ca2+ visualization ...
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bioRxiv - Biophysics 2019Quote: ... for biplane analysis were obtained by running a Z stack of 200 steps (with an axial step size 50 nm) of sparse sub-resolution (200 nm) dark-red fluorescent beads (ThermoFisher FluoSpheres F8807). A number of such beads are selected ...
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bioRxiv - Microbiology 2019Quote: ... of representative isolates from each dendrogram cluster was used as template in cycle sequencing reactions using both pA and pH primers with fluorescent dye-labelled terminators (Applied Biosystems, USA). The sequencing was performed in a 3130xl Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... patch pipettes were filled with the same cesium-base solution described above supplemented with 50 µM of the red fluorescent indicator Rhod2 (R14220, Invitrogen™, Thermofisher Scientific). The Ca2+ dye was excited with a 543 nm Helium Neon Laser system (Melles Griot 05-LGR-193-381 ...
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bioRxiv - Biophysics 2021Quote: ... Fluorescent actin was prepared by labelling monomers with Alexa Fluor 649 carboxylic acid succinimidyl esther (Molecular Probes, Life Technologies, Carlsbad, CA, USA). Before use ...
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bioRxiv - Biophysics 2021Quote: ... Fluorescent actin was prepared by labelling monomers with Alexa Fluor 649 carboxylic acid succinimidyl esther (Molecular Probes, Life Technologies, Carlsbad, CA, USA). Before use ...
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bioRxiv - Microbiology 2020Quote: ... 1.3 µL of 1 mg/mL stock of the lipophilic fluorescent dye BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene, 493/503, Molecular Probes, ThermoFisher Scientific, USA) per sample was added to stain for neutral lipids (TAG) ...
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bioRxiv - Cell Biology 2021Quote: ... The channels were then carefully aligned using alignment parameter from control measurements with 0.5 μm diameter multi-spectral fluorescent beads (Invitrogen, Thermo Fisher Scientific). For visualization of the Golgi surface ...
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bioRxiv - Biophysics 2020Quote: ... The biotinylated surface was then incubated with FluoSpheres™ NeutrAvidin™-marked microspheres (200nm in diameter, fluorescent in yellow-green, ThermoFisher, F8774) with 1-5uL in 150uL PBS per hydrogel ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane potential was determined by incubating 300’000 cells for 40 minutes with 100 nM of the fluorescent probe DiBAC4(3) (Thermofisher, ref. no. B438) in 6-well plates in 1 mL media ...
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bioRxiv - Biochemistry 2022Quote: ... At culture day 8 cytosolic Ca2+ levels in primary neurons were determined using the Ca2+-sensitive fluorescent indicator Fura-2-AM (Molecular Probes/Invitrogen). Cells were loaded with Fura-2 in sterile-filtered HEPES-buffered saline (HBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed x3 in PBS or TBS and incubated with fluorescent secondary antibodies and dyes (DAPI, Cell Signaling Technology; Phalloidin, ThermoFisher) in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated for one hour at room temperature with secondary antibodies conjugated with Alexa Fluor fluorescent probes (Thermo Fisher Scientific) diluted in 10 % horse serum ...
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bioRxiv - Microbiology 2020Quote: ... followed by qPCR using HIV-1 primers and probes (specific for transcription of the first nucleosome nuc1a or gag/u1a) and the housekeeping genes 18S and GAPDH (both containing VIC&trade/TAMRA&trade fluorescent probe; Applied Biosystems, USA) as controls (see list of primers).
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... cells were incubated with 0.5 μM tetramethylrhodamine (TMRM; a cell permeant fluorescent red-orange dye as mitochondrial membrane potential indicator) (Thermo Fisher Scientific,T668) and Hoechst dye (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... 500 μL of exponentially growing cells in TY were centrifuged and resuspended in 100 μL of PBS supplemented with the fluorescent membrane dye FM 4-64 (Molecular Probes, Invitrogen) at 1 μg/mL ...
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bioRxiv - Neuroscience 2020Quote: Each section of the mOFC was collected directly onto a slide and was stained with NeuroTrace fluorescent nissl (Invitrogen, Eugene, OR, USA) and left to dry in darkness for at least 30 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell invasion distance was measured from the bead surface using ImageJ from images acquired via fluorescent microscopy after fixing and staining cells with DAPI (Invitrogen, Carlsbad, CA) (10 µg/mL in 1X PBS) ...
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bioRxiv - Plant Biology 2019Quote: Membrane permeabilization of fungal cells was analyzed using confocal microscopy by visualizing the influx of the fluorescent dye SYTOX Green (SG) (Thermo Fisher Scientific). Fresh conidia or germlings mixed with 2 x MIC (3 µM for B ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Genotyping was performed with four-color fluorescent capillary electrophoresis using an Applied Biosystem Prism 3100 sequencer (Applied Biosystems, Foster City, CA, USA), and alleles were scored using GENEMAPPER v4.0 ...
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bioRxiv - Cell Biology 2019Quote: ... Processed SIM images were aligned via an affine transformation matrix of predefined values obtained using 100 nm multicolor Tetraspeck fluorescent microspheres (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... Gene-edited U2OS cells expressing Monocarboxylate Transporter 1 fluorescently labelled with Enhanced Green Fluorescent Protein (MCT1-EGFP) in both alleles were grown in DMEM/F12/GlutaMAX (Cat. No. 10565042, Gibco, Waltham, MA) supplemented with 10% fetal bovine serum (Cat ...
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bioRxiv - Genetics 2019Quote: ... Fragment length was detected by fluorescent capillary fragment analysis on an ABI 3730s Genetic Analyzer and all data analyzed using the GeneMapper software (all Thermo Fisher Scientific). Data was quality controlled for null allele overrepresentation and deviation from Hardy-Weinberg Equilibrium using Micro-Checker(45 ...
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bioRxiv - Zoology 2020Quote: We examined the PVL for sperm and germinal disc samples for embryonic cells by staining them with the fluorescent DNA marker Hoechst 33342 (Thermo Fisher Scientific), and inspecting them under a fluorescence microscope with a BP 340-380 excitation filter and LP 425 suppression filter (Birkhead et al ...
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bioRxiv - Genomics 2020Quote: ... diffraction- limited images of spatially separated broad-range emitting fluorescent beads were acquired across all three colors (Tetraspecks, 100 nm, Life Technologies, T7279). The precise localizations of the beads were obtained by independently fitting their diffraction-limited Point Spread Functions (PSF ...
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bioRxiv - Microbiology 2020Quote: Treated and untreated biofilm-containing pegs after a 24-hour incubation in MMBC-3 were subjected to microscopic imaging after staining with 5 μM of Syto®9 green-fluorescent nucleic acid stain (Invitrogen, ThermoFisher Scientific) diluted in PBS ...
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bioRxiv - Microbiology 2021Quote: ... surface lysines on MS2 capsids were modified with fluorescent dyes (NHS-Atto488 or NHS-Atto633) and the heterobifunctional maleimide-PEG24-N-hydroxysuccinimide ester crosslinker (Thermo Fisher Scientific). Second ...