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Citations for Thermo Fisher :
4651 - 4700 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... secondary antibodies conjugated with fluorescent dyes were directly visualised at 700 or 800 nm (anti-mouse 800, Invitrogen, SA5-10172; anti-rabbit 680, ThermoFisher, 35569). Western blot quantification was performed using LI-COR image studio ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were then washed with DPBS thrice and observed immediately under a fluorescent microscope (Evos FL Color Imaging System from Thermo Scientific). Five random microscopic fields were selected and the intensity of fluorescence was quantified using the Image J software ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were washed 3 times in 1x PBS and primary antibodies were detected using species- specific fluorescent secondary antibodies (Life Technologies). Samples were washed 3 more times in 1x PBS before DNA detection with 1 µg/ml Hoechst 33342 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Real-Time RT-PCR Diagnostic Panel (https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html) except that the probe quencher was modified to Non-Fluorescent Quencher-Minor Groove Binder (NFQ-MGB) (Thermo Fisher Scientific). A standard curve comprised of synthetic RNA containing the target sequence from SARS-CoV-2 isolate WA1 sequence (GenBank Accession Number MN985325.1 ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting suspension with all nuclei was stained with the fluorescent DNA intercalant marker DAPI (4’-6-diamidino-2-phenylindole dihydrochloride, Invitrogen, USA) diluted 1:20 from a stock solution of 20 mg/L and filled with PBS 0.1M to known volume ...
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bioRxiv - Biophysics 2019Quote: ... the plated hiPSC-CMs were loaded with a Ca2+-sensitive fluorescent dye (Fura-2 AM, cell permeant, ThermoFisher, Rockville, MD, USA), and the ratio of fluorescence intensities (excitation ratio of 340/380 nm ...
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Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplicationbioRxiv - Cell Biology 2019Quote: ... Color channels were carefully aligned using alignment parameter from control measurements with 0.5 µm diameter multi-spectral fluorescent beads (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2019Quote: ... the unstained control condition was verified as having equivalent fluorescent reads as a mouse IgG2b K Isotype Control APC antibody (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2019Quote: ... The lipophilic fluorescent probes DiD (excitation 644nm, emission 665nm) and DiO (excitation 484nm, emission 501nm) Vybrant cell labelling solutions were from Molecular Probes, Life Technologies (Grand Island ...
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bioRxiv - Cell Biology 2019Quote: ... or two independent shRNAs targeting CHCHD4 (CHCHD4 shRNA1 or CHCHD4 shRNA2) utilizing a green fluorescent protein (GFP)-SMARTvector™ pre-packaged lentivirus system from ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2020Quote: Fluorescent images of each well at 10x and 20x were obtained using an EVOS FL Cell Imaging System (Invitrogen, model AMF4300) using the RFP channel (excitation/emission ...
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bioRxiv - Cell Biology 2019Quote: ... Confocal channel shift alignment and STORM point spread function (PSF) calibration and channel shift alignment were performed using 0.1μ m TetraSpeck fluorescent beads (Invitrogen, cat#T7279).
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bioRxiv - Neuroscience 2019Quote: ... To preserve a more intact hair cell intracellular milieu we also performed perforated-patch recordings using an internal solution with gramicidin (40-50 μg/ml; Fig. 5A and 6) plus a fluorescent dye (Alexa Fluor 488 Hydrazide; ThermoFisher Scientific). Hair cells containing dye in their cytoplasm were excluded from analysis ...
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bioRxiv - Genetics 2020Quote: ... membranes were incubated with the corresponding fluorescent secondary antibody (either goat anti-rabbit IgG, Invitrogen #A32732 or goat anti-mouse IgG-Invitrogen #A28175). Membranes were then washed with TBST and imaged on (ODYSSEY CLx).
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bioRxiv - Molecular Biology 2020Quote: ... the dried peptides were re-dissolved in 100 mM TEAB (50 ml) and their concentration was measured using a fluorescent assay (CBQCA, Life Technologies). 100 mg of peptides from each cell line to be compared ...
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bioRxiv - Neuroscience 2020Quote: ... images were processed with Zen 2012 using the SIM reconstruction module with default settings and drift corrections between the channels were performed with respect to 100nm Tetraspec fluorescent microspheres (Molecular probes). To create kymographs image sequences were opened within ImageJ ...
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bioRxiv - Microbiology 2021Quote: All pools were sent to University of Texas Medical Branch for library preparation where total RNA was quantified using a Qubit fluorescent assay (Thermo Scientific) and RNA quality was assessed using an RNA 6000 chip on an Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... Viral copy number quantification by RT-qPCR was performed using Detection Kit for 2019 Novel Coronavirus (2019-nCoV) RNA (PCR-Fluorescent Probing) (Cat. #DA-930) (China) in a QuantStudio 3 real-time PCR system (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Expression vectors containing the promoter-gene-fluorescent tag cassette or 3’UTR were obtained by using LR clonase-based three-fragment recombination system (Invitrogen®), the pB7m34GW/pH7m34GW/pK7m34GW/pS7m43GW/ pLOK180_pR7m34g (gift from L ...
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bioRxiv - Biochemistry 2021Quote: ... Cell surface display DNA constructs for the SARS-CoV-2 spike variants together with a plasmid expressing blue fluorescent protein (BFP) were transiently transfected into Expi293F cells using ExpiFectamine 293 reagent (ThermoFisher Scientific) per manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: Cells were prewashed with PBS and incubated with 500 mM SYTO RNASelect Green Fluorescent Cell Stain (S32703, Thermo Fisher Scientific, USA) for 20 min at 37°C ...
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bioRxiv - Systems Biology 2021Quote: Chromatic aberration shifts between different fluorescent channels were corrected by applying the calculated offsets using the fiducial markers or 0.2 μm TetraSpeck Microspheres (Thermo Scientific T7280) appeared in each fluorescent channel.
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bioRxiv - Microbiology 2021Quote: ... The vesicles were formed encapsulating the fluorescent membrane-impermeable dye 8-hydroxypyrene-1,3,6-trisulfonic acid from Thermo Fisher (HPTS, 50 μM). The microfluidic platform was operated by two positive pressure-driven pump modules (MFCS-4C ...
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bioRxiv - Cancer Biology 2020Quote: ... the RNA of MDA-MB-231 was stained by SYTO RNASelect green fluorescent cell stain (Thermo Fisher Scientific, Cat No. S32703) at 1:10,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then incubated for 5 min with a filtered (220 nm, Millex® - GP) and sonicated solution of 200-nm-diameter red fluorescent carboxylate-modified beads (FluoSpheres, Invitrogen) in sodium tetraborate (3.8 mg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... The channels were then carefully aligned using alignment parameter from control measurements with 0.5 μm diameter multi-spectral fluorescent beads (Invitrogen, Thermo Fisher Scientific). For visualization of the Golgi surface ...
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bioRxiv - Cell Biology 2021Quote: ... cells were re-suspended once in DPBS and the expressed GFPs fluorescent intensity was measured by flow cytometry (Attune Nxt, Thermo Fisher).
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bioRxiv - Biochemistry 2022Quote: ... At culture day 8 cytosolic Ca2+ levels in primary neurons were determined using the Ca2+-sensitive fluorescent indicator Fura-2-AM (Molecular Probes/Invitrogen). Cells were loaded with Fura-2 in sterile-filtered HEPES-buffered saline (HBS ...
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bioRxiv - Developmental Biology 2022Quote: ... For epifluorescence microscopy, embryos were imaged with an Echo Revolve fluorescent microscope (Echo, USA) or an EVOS M5000 Imaging System (ThermoFisher, USA) employing a LPanFL PH2 20X/0.40 lens ...
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bioRxiv - Neuroscience 2022Quote: ... The shanks of the probes were marked with a fluorescent dye (DiI: Cat# V22885, or DiD: Cat# V22887, Thermo-Fisher Scientific) before penetration of the brain to assist in the identification of shank location postmortem ...
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bioRxiv - Bioengineering 2022Quote: ... Cellular two-phase ADCs seeded with fluorescent expressing IRM90 (mCherry) and MDA-MB-231 (GFP) were observed at 2x using EVOS M5000 (ThermoFisher, Canada) through the GFP and Texas Red channels ...
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bioRxiv - Cancer Biology 2022Quote: ... BM adipocytes were quantified by intracellular staining of the FBM lipid with LipidTox (fluorescent dye that stains neutral lipids; Life technologies) and analyzed using ImageStream X Mark II ...
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bioRxiv - Cancer Biology 2022Quote: ... cocultured mCherry+/CD133high cells and Wasabi+/SCCs were processed to single cells and labeled with fixable live/dead near-infrared fluorescent reactive dye (Invitrogen, L34975). The percentages of dead cells (live/dead dye+ ...
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bioRxiv - Microbiology 2022Quote: ... fluorescent labeling of the OPP was made with the Click-ITTM Plus Alexa FluorTM 488 Picolyl Azide (Thermo Fisher Scientific, C10641), according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... Cell surface display DNA constructs for the SARS-CoV-2 spike variants together with a plasmid expressing blue fluorescent protein (BFP) were transiently transfected into Expi293F cells using ExpiFectamine 293 reagent (ThermoFisher Scientific) per manufacturer’s instruction ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and a lab-made cytomegalovirus promoter-driven enhanced green fluorescent protein (GFP) reporter construct using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). On day three ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then loaded at room temperature for 30 min with fluorescent indicator-linked substrates for activated caspase-8 (10 µM Z-IETD-R110; Molecular Probes), caspase-9 (10 µM ZLEHD-R110 ...
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bioRxiv - Microbiology 2020Quote: ... 500 μL of exponentially growing cells in TY were centrifuged and resuspended in 100 μL of PBS supplemented with the fluorescent membrane dye FM 4-64 (Molecular Probes, Invitrogen) at 1 μg/mL ...
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bioRxiv - Immunology 2020Quote: The following fluorescent reporters were added to the cell cultures 15 mn before ATP stimulation: Hoechst 33342 (10 μg/mL) (Invitrogen, H3570), LysoTracker Green DND-26 (1 μM ...
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bioRxiv - Cancer Biology 2021Quote: MDA-MB-231 were transfected with a lentiviral vector encoding the red fluorescent protein palmitoylated-tdTomato (tdTomato) using lipofectamine transfection reagent (Life Technologies). Supernatant recovered after 48h from 293T transfected cells was filtered by a 0.45□μm pore membrane and added to MDA-MB-231 plated cells supplemented with 4□μg/mL polybrene (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... The bacteria were then washed once to remove the primary antibody and then stained with an anti-mouse fluorescent secondary conjugated with AlexaFluor 647 (Thermo Fisher). Fibronectin binding was assessed by flow cytometry (CytoFlex ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then loaded at room temperature for 30 min with fluorescent indicator-linked substrates for activated caspase-8 (10 μM Z-IETD-R110; Molecular Probes), caspase-9 (10 μM Z-LEHD-R110 ...
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bioRxiv - Microbiology 2019Quote: ... The fixed cells were observed for fluorescent-positive syncytia using an EVOS FL Auto 2 Cell Imaging System (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... the cells were incubated with 1 μM cell membrane permeable Fluozin-3 acetoxy-methyl ester (AM) fluorescent dye (MP-FZ3; Kd =15 nM; ex = 494 nm, em = 516 nm; Thermo Scientific). Fluozin-3 is a well-established tool for evaluating changes in relative zinc levels due to its specificity ...
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bioRxiv - Cell Biology 2019Quote: ... Intracellular parasite mitochondrial staining was carried out using the protocol previously described (Salunke et al., 2018) with the fluorescent dye MitoTracker® red CMXRos (300 nM) from Invitrogen™.
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bioRxiv - Bioengineering 2020Quote: Carboxylate modified red fluorescent polystyrene spheres with 100 nm and 500 nm diameter (Life Technologies; Excitation 530 nm & Emission 590 nm) were coated with a high surface density of polyethylene glycol (PEG ...
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bioRxiv - Bioengineering 2020Quote: ... De-skewed volumes were then deconvolved with Slidebook using the constrained iterative deconvolution algorithm and an experimental point spread function generated with 200 nm fluorescent beads (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... Fluorescence emission spectrum for these samples were acquired at 510-520nm by using a fluorescent microplate reader (Multiskan O Microplate Spectrophotometer, ThermoFisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... Next cells were harvested from each experimental condition and the luciferase activity was measured using a fluorescent microplate reader (Multiskan O Microplate Spectrophotometer, ThermoFisher Scientific). Final activity was calculated as the ratio of firefly luciferase activity versus renilla luciferase activity units.
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bioRxiv - Cell Biology 2020Quote: ... GH+SB431542 and GH+AG490 for 48 hr and cleavage-dependent release of APH-1A measured at 450 nm by using a fluorescent microplate reader (Multiskan O Microplate Spectrophotometer, ThermoFisher Scientific). For TGF-β1 detection in condition media from HPC treated with different experimental conditions and in urine samples from mice and human were analyzed by TGF-β1 ELISA commercial kit (R&D Systems ...