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Citations for Thermo Fisher :
4801 - 4850 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... Expression levels of genes was quantified using qRT-PCR and the QuantStudio™5 Real-Time PCR system (Thermo Fisher). qRT-PCR was performed using the indicated qPCR primers (Supplementary Table 11) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was used to perform RRT-PCR reactions in the ABI 7300 Real-Time PCR System (Applied Biosystems, Foster City, CA). Each reaction contained 12.5 µL of kit-supplied 2X RT-PCR Master mix ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were diluted 10x and qRT-PCR reactions were performed using a QuantStudio 3 Real-Time PCR System (Applied Biosystems). For each reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products derived from ChIP-DNA were quantified by real-time PCR using the Fast ABI 7500 instrument (Applied Biosystems). The reaction mixture contained 2 µl of ChIP or input DNA (diluted 1:10 to represent 1% of input DNA) ...
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bioRxiv - Molecular Biology 2024Quote: Quantitative PCR was performed with The StepOne™ Real-Time PCR System or QuantStudio™ 3 System (Applied Biosystems™) using 5 μl diluted cDNA ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR reaction was carried out in 96-well plates using the 7500 Fast Real-Time PCR System (Applied Biosystems). A melting curve analysis was performed for each sample to verify PCR specificity ...
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bioRxiv - Cancer Biology 2024Quote: ... and qRT-PCR was performed using SYBR Green Master Mix on a StepOnePlus Real-time PCR system (Thermo Fisher Scientific). Gene expression was normalized to 18s rRNA ...
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bioRxiv - Developmental Biology 2024Quote: ... The Q-PCR were performed using the StepOne real-time PCR system and following its standard amplification protocol (Thermo Scientific). Relative gene expression levels were quantified using the comparative CT method (2-ΔΔCT method or 2-ΔCT ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... cDNA samples were diluted 5-folds and PCR was performed with StepOne Plus Real-Time PCR System (PE Applied Biosystems). The FastStart Universal SYBR Green Master (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time quantitative PCR was performed on 1/10 dilution of cDNA using SYBR green PCR master mix (Fisher Scientific) and the primers listed in Appendix Table S1.
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bioRxiv - Immunology 2024Quote: ... qRT-PCR was conducted using the Applied Biosystems One-Step Real-time PCR protocol using SYBR Green fluorescence (Applied Biosystems) on an Applied Biosystems 7900HT real-time PCR machine in 96-well-plate format ...
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bioRxiv - Immunology 2024Quote: ... Quantitative real-time PCR (qPCR) was performed using specific primers (0.2µM concentration and SYBR Green PCR Master Mix (Applied Biosystems, #4309155) on the Mastercycler RealPlex2 (Eppendorf) ...
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bioRxiv - Microbiology 2023Quote: ... The resulting cDNA was utilized as input for quantitative PCR analysis on a StepOnePlus Real Time PCR System (Applied Biosystems) using the PowerUp SYBR Green Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR was conducted with a QuantStudio real-time PCR system or an ABI Prism system (both from Applied Biosystems), with the following experimental settings ...
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bioRxiv - Neuroscience 2023Quote: ... All qRT-PCR analyses were performed on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific). Target mRNA expression was normalised for RNA loading using Gapdh (Integrated DNA Technologies) ...
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bioRxiv - Physiology 2023Quote: qRT-PCR was conducted using the Applied Biosystems One-Step Real-time PCR protocol using SYBR Green fluorescence (Applied Biosystems) on an Applied Biosystems 7900HT real-time PCR machine in 96-well-plate format ...
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bioRxiv - Neuroscience 2023Quote: ... All qRT-PCR analyses were performed on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific). Target mRNA expression was normalised for RNA loading using Gapdh (Integrated DNA Technologies) ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using the SYBR Green SuperMix and StepOne Software v.2.3 (Applied Biosystems) with 40 cycles of amplification (including three biological replicates) ...
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bioRxiv - Genomics 2023Quote: ... 10-fold diluted cDNA was used for quantitative Real-time PCR with Power SYBR Green PCR Master Mix (4367659, Invitrogen) master mix with 500nM forward and reverse primer pairs (Supplementary table 1 ...
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bioRxiv - Biochemistry 2023Quote: ... Quantitative PCR (qPCR) was performed on a StepOne™ Real-Time PCR System (Applied Biosystems, Themo Fisher Scientific, CAT# 4376598) using Maxima SYBR Green qPCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Quantitative PCR (qPCR) was performed on a StepOne™ Real-Time PCR System (Applied Biosystems, Themo Fisher Scientific, CAT# 4376598) using Maxima SYBR Green qPCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and used for q-PCR in a Viia7 Real-time PCR thermal cycler using both Fast SYBR Green Master Mix (ThermoFisher), after standard curve method optimization to reach the 100% primer efficiency for each couple of primers listed in Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... and quantitative PCR (qPCR) was performed with Thunderbird Next SYBR qPCR mix (Toyobo #QPX-201) and StepOnePlus real-time PCR system (ThermoFisher) following manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was carried out on the Applied Biosystems 7500 Real Time PCR system using Power SYBRgreen PCR master mix (Invitrogen), 2 ng of 1:10 diluted cDNAs per reaction and the following cycling conditions ...
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bioRxiv - Physiology 2023Quote: ... The qRT-PCR was carried out using the Applied Biosystems QuantStudio3 real-time PCR machine (Thermo Fisher, Waltham, MA, USA). For data analysis ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was analyzed for gene expression with quantitative PCR using a Quant Studio 3 real-time PCR system (Applied Biosystems). Forward and reverse primers for each gene analyzed are listed in Supplementary Table 4 ...
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bioRxiv - Immunology 2024Quote: ... and real-time PCR was performed with PowerUp SYBR Green PCR Master Mix in QuantStudio 5 system (Thermo Fisher Scientific). Relative fold change was calculated with 2−ΔΔCt methods ...
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bioRxiv - Neuroscience 2024Quote: ... The quantitative real-time polymerase chain reaction (PCR) was conducted using a Fast SYBR Green PCR Master Mix (Applied Biosystems) along with specific primers for Oxtr and GAPDH genes (HyLabs Israel Ltd.) ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR products were amplified from extracted RNA using a SuperScript™ III One-Step RT-PCR kit (Invitrogen, Carlsbad, CA, USA). A 20 μl reaction was assembled in PCR 8-tube strips through the addition of 10 μl 2× reaction mix ...
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bioRxiv - Microbiology 2024Quote: ... the extracted vRNA was converted to double-stranded cDNA and amplified using a one-step RT-PCR using SuperScript III One-Step RT-PCR kit (Thermo Fisher Scientific). The primers used were as follows ...
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bioRxiv - Microbiology 2024Quote: The viral genome was amplified from RNA using a multi-segment one-step RT-PCR with Superscript III high-fidelity RT-PCR kit (Invitrogen, Carlsbad CA) according to manufacturer’s instructions using the Opti1 primer set (Opti1-F1 ...
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bioRxiv - Developmental Biology 2020Quote: ... and cDNA was produced using RT-PCR kit (K1612, Thermo Scientific). For qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized using an RT-PCR kit (Thermo Scientific, USA).
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bioRxiv - Molecular Biology 2019Quote: ... RT-PCR was performed using FAST SYBR kit (4385616, Applied Biosystems) with three technical replicates and analyzed on an Applied Biosystems 7500 FAST and Real-Time PCR system ...
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bioRxiv - Microbiology 2022Quote: ... RT-PCR was carried out using a reverse transcriptase kit (Invitrogen) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... the SuperScriptIII First-Strand Synthesis System for RT-PCR kit (Invitrogen) was used ...
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bioRxiv - Microbiology 2019Quote: ... One step RT-PCR kit (Thermo Fisher Scientific, Waltham, MA, USA) on the Applied Biosystems 7500 FAST following the previously described protocols.
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bioRxiv - Microbiology 2022Quote: ... using a SuperScript III One-Step RT-PCR Kit (Invitrogen, Germany). Reverse transcription of the viral RNA and amplification of the cDNA was conducted in a LightCycler 480 System (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... was retro-transcribed to cDNA using RT-for-PCR kit (Invitrogen, Life technologies ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was measured by quantitative real real-time polymerase chain reaction (qRT-PCR) using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with primers and probe specific for the CCHFV L gene.
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bioRxiv - Genomics 2021Quote: ... was used to perform qRT-PCR analysis of miR-29 and miR-26 using TaqMan Fast Advanced miRNA Assays (ThermoFisher, 4444557) following guidelines provided by the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... quantitative RT-PCR analysis was performed using a sequence detection system (ABI ViiA 7 system; Thermal Fisher Scientific). The following primers sets were used for analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... SYBR green-based RT-PCR was performed using the 7900HT Sequence Detection System (Applied Biosystems, Foster City, California) and respective primers ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: Quantitative RT-PCR was performed in duplo on an ABI Prism 7900HT Sequence Detection System (PE Applied Biosystems) or StepOnePlus Real-Time PCR System (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... TaqMan quantitative RT-PCR analysis was performed using the ABI prism 7500 Sequence Detection System (Applied Biosystems,CA) with specific FAM-labeled probes ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA samples went through Reverse Transcription-PCR (RT-PCR) using the cDNA kit (Applied Biosystems). cDNA samples for quantitative Real Time PCR were prepared using a mix of 1X Fast-SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed using the SuperScript III One-Step RT-PCR kit (ThermoFisher Scientific) on a QuantStudio 7 Flex System (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... qRT-PCR reactions were then set up using RT-PCR kit (Applied Biosystems, ThermoFisher Scientific). Primers used are listed in Table S3.
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bioRxiv - Bioengineering 2021Quote: ... qRT-PCR reactions were then set up using RT-PCR kit (Applied Biosystems, ThermoFisher Scientific). Primers used are listed in Table S3.
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bioRxiv - Cancer Biology 2023Quote: ... followed by RT-PCR reaction using PowerUp SYBR Green PCR Master Kit (Applied Biosystems #A25742) in QuantStudio 5 Systems (Applied Biosystems #4309155) ...